FN ISI Export Format VR 1.0 PT J AU Colombat, P Salles, G Brousse, N Eftekhari, P Soubeyran, P Delwail, V Deconinck, E Haioun, C Foussard, C Sebban, C Stamatoullas, A Milpied, N Boue, F Taillan, B Lederlin, P Najman, A Thieblemont, C Montestruc, F Mathieu-Boue, A Benzohra, A Solal-Celigny, P TI Rituximab (anti-CD20 monoclonal antibody) as single first-line therapy for patients with follicular lymphoma with a low tumor burden: clinical and molecular evaluation SO BLOOD ID NON-HODGKINS-LYMPHOMA; B-CELL LYMPHOMA; A CHAIN IMMUNOTOXIN; PHASE-II TRIAL; IDEC-C2B8; CHEMOTHERAPY; COMBINATION; INTERFERON; INFUSION; BLOOD AB The clinical activity of rituximab, a chimeric monoclonal antibody which binds to the CD20 antigen, was evaluated as a single first-line therapy for patients with follicular non-Hodgkin lymphoma (NHL). Fifty patients with follicular CD20(+) NHL and a low tumor burden were analyzed for clinical and molecular responses, They received 4 weekly infusions of rituximab at a dose of 375 mg/m(2). The response rate a month after treatment (day 50) was 36 of 49 (73%), with 10 patients in complete remission, 3 patients in complete remission/unconfirmed, and 23 patients in partial remission. Ten patients had stable disease, and the disease progressed in 3 patients, One of 13 (8%) patients in complete remission, 9 of 23 (39%) patients in partial remission, and 5 of 10 (50%) patients with stable disease exhibited disease progression during the first year. Within the study population, 32 patients were initially informative for polymerase chain reaction (PCR) data on bcl-2-J(H) rearrangement. On day 50, 17 of 30 patients (57%) were negative for bcl-2-J(H) rearrangement in peripheral blood, and 9 of 29 (31%) were negative in bone marrow; a significant association was observed between molecular and clinical responses (P < .0001), At month 12, 16 of 26 patients (62%) were PCR negative in peripheral blood. These results indicate that early molecular responses can be sustained for up to 12 months and that this response is highly correlated with progression-free survival. Rituximab has a high clinical activity and a low toxicity and induces a high complete molecular response rate in patients with follicular lymphoma and a low tumor burden. (C) 2001 by The American Society of Hematology. C1 Ctr Jean Bernard, F-72000 Le Mans, France. Ctr Hosp Lyon Sud, F-69310 Pierre Benite, France. CHU Bretonneau, F-37044 Tours, France. Hop Necker Enfants Malad, Paris, France. Hop St Louis, Paris, France. Hop St Antoine, F-75571 Paris, France. Inst Bergonie, Bordeaux, France. CHU La Miletrie, Poitiers, France. Hop Jean Minjoz, F-25030 Besancon, France. Hop Henri Mondor, F-94010 Creteil, France. CHU Angers, Angers, France. Ctr Leon Berard, F-69373 Lyon, France. Ctr Henri Becquerel, F-76038 Rouen, France. CHU Hotel Dieu, Nantes, France. Hop Antoine Beclere, Clamart, France. Hop Larchet, Nice, France. CHU Nancy Brabois, Vandoeuvre Nancy, France. Prod Roche, Neuilly Sur Seine, France. RP Solal-Celigny, P, Ctr Jean Bernard, 9 Rue Beauverger, F-72000 Le Mans, France. TC 186 PD JAN 1 PY 2001 VL 97 IS 1 BP 101 EP 106 UT ISI:000166177300012 ER PT J AU Major, P Lortholary, A Hon, J Abdi, E Mills, G Menssen, HD Yunus, F Bell, R Body, J Quebe-Fehling, E Seaman, J TI Zoledronic acid is superior to pamidronate in the treatment of hypercalcemia of malignancy: A pooled analysis of two randomized, controlled clinical trials SO JOURNAL OF CLINICAL ONCOLOGY ID CANCER-ASSOCIATED HYPERCALCEMIA; TUMOR-ASSOCIATED HYPERCALCEMIA; AMINOHYDROXYPROPYLIDENE DIPHOSPHONATE; DOSE-RESPONSE; BISPHOSPHONATES; MECHANISMS; DISODIUM; TOLERABILITY; PHARMACOLOGY; INFUSIONS AB Purpose: Two identical, concurrent, parallel, multicenter, randomized, double-blind, double-dummy trials were conducted to compare the efficacy and safety of zoledronic acid and pamidronate for treating hypercalcemia of malignancy (HCM). Patients and Methods: Patients with moderate to severe HCM (corrected serum calcium [CSC] greater than or equal to 3.00 mmol/L [12.0 mg/dL]) were treated with a single dose of zoledronic acid (4 or 8 mg) via 5-minute infusion or pamidronate (90 mg) via 2-hour infusion. A protocol-specified pooled analysis of the two parallel trials was performed. Clinical end points included rate of complete response by day 10, response duration, and time to relapse. Results: Two hundred eighty-seven patients were randomized and evaluated for safety,. 275 were evaluated for efficacy. Both doses of zoledronic acid were superior to pamidronate in the treatment of HCM. The complete response rates by day 10 were 88.4% (P =.002), 86.7% (P =.015), and 69.7% for zoledronic acid 4 mg and 8 mg and pamidronate 90 mg, respectively. Normalization of CSC occurred by day 4 in approximately 50% of patients treated with zoledronic acid and in only 33.3% of the pamidronate-treated patients. The median duration of complete response favored zoledronic acid 4 and 8 mg over pamidronate 90 mg with response durations of 32, 43, and 18 days, respectively. Conclusion: Zoledronic acid is superior to pamidronate; 4 mg is the dose recommended for initial treatment of HCM and 8 mg for relapsed or refractory hypercalcemia. J Clin Oncol 19:558-567. (C) 2001 by American Society of Clinical Oncology. C1 Hamilton Reg Canc Ctr, Hamilton, ON L8V 5C2, Canada. Ctr Paul Papin, Angers, France. Comprehens Canc Inst, Huntsville, AL 35801 USA. Bendigo Hosp, Bendigo, Australia. Andrew Love Canc Ctr, Geelong, Vic, Australia. Feist Weiller Canc Ctr, Shreveport, LA 71101 USA. Free Univ Berlin, Klinikum Benjamin Franklin, D-12200 Berlin, Germany. Boston Canc Grp, Memphis, TN 38146 USA. Inst Jules Bordet, B-1000 Brussels, Belgium. Novartis Pharma, Basel, Switzerland. RP Major, P, Hamilton Reg Canc Ctr, 699 Concess St, Hamilton, ON L8V 5C2, Canada. TC 146 PD JAN 15 PY 2001 VL 19 IS 2 BP 558 EP 567 UT ISI:000166534000036 ER PT J AU Facon, T Avet-Loiseau, H Guillerm, G Moreau, P Genevieve, F Zandecki, M Lai, JL Leleu, X Jouet, JP Bauters, F Harousseau, JL Bataille, R Mary, JY CA Intergroupe Francophone Myelome TI Chromosome 13 abnormalities identified by FISH analysis and serum beta(2)-microglobulin produce a powerful myeloma staging system for patients receiving high-dose therapy SO BLOOD ID IN-SITU HYBRIDIZATION; BONE-MARROW TRANSPLANTATION; C-REACTIVE PROTEIN; MULTIPLE-MYELOMA; PROGNOSTIC VALUE; UNDETERMINED SIGNIFICANCE; MONOCLONAL GAMMOPATHY; CYTOGENETICS; SURVIVAL; BETA-2-MICROGLOBULIN AB A careful prognostic evaluation of patients referred for high-dose therapy (HDT) is warranted to identify those who maximally benefit from HDT as well as those who clearly fail current HDT and are candidates for more innovative treatments. In a series of 110 patients with myeloma who received HDT as first-line therapy, times to event (disease progression and death) were studied through proportional hazard models, in relation to different prognostic factors, including a chromosome 13 fluorescence in situ hybridization (FISH) analysis using a D13S319 probe. Delta 13 was detected in 42 patients (38%). Follow-up time among surviving patients and survival time were 48 +/- 3 and 51 +/- 7 months, respectively (median +/- SE). In the univariate analysis, Delta 13 was the most powerful adverse prognostic factor for all times to event, especially for the survival time (P < .0001) and was followed by (2)-microglobulin (beta (2)m) levels 2.5 mg/L or higher (P = .0001). The comparison of survival prognostic models including beta (2)m 2.5 mg/L or greater and another factor favored the Delta 13/beta (2)m combination. In 22 patients (20%) with no unfavorable factor, the median survival time was not reached at 111 months. In contrast, among 55 patients (50%) with one unfavorable factor and 33 patients (30%) with 2 unfavorable factors, median survival times were 47.3 +/- 4.6 months and 25.3 +/- 3.2 months, respectively (P < .0001). We conclude that 13, adequately detected by FISH analysis, is a very strong factor related to poor survival, especially when associated with a beta (2)m level of 2.5 mg/L or higher. Routine FISH Delta 13 assessment is strongly recommended for patients considered for HDT. (Blood, 2001;97:1566-1571) (C) 2001 by The American Society of Hematology. C1 Serv Hematol, Lille, France. Med Genet Lab, Lille, France. Serv Hematol, Nantes, France. Hematol Lab, Nantes, France. Hematol Lab, Angers, France. Univ Paris 07, Equipe Biostat Biomath, Paris, France. RP Facon, T, Hop Claude Huriez, Serv Malad Sang, F-59037 Lille, France. TC 122 PD MAR 15 PY 2001 VL 97 IS 6 BP 1566 EP 1571 UT ISI:000167406800010 ER PT J AU Hoekstra, FA Golovina, EA Buitink, J TI Mechanisms of plant desiccation tolerance SO TRENDS IN PLANT SCIENCE ID HEAT-SHOCK PROTEINS; CRATEROSTIGMA-PLANTAGINEUM; SACCHAROMYCES-CEREVISIAE; MOLECULAR MOBILITY; LEA PROTEINS; SEEDS; DEHYDRATION; EXPRESSION; STRESS; STABILITY AB Anhydrobiosis ('life without water') is the remarkable ability of certain organisms to survive almost total dehydration. It requires a coordinated series of events during dehydration that are associated with preventing oxidative damage and maintaining the native structure of macromolecules and membranes. The preferential hydration of macromolecules is essential when there is still bulk water present, but replacement by sugars becomes important upon further drying. Recent advances in our understanding of the mechanism of anhydrobiosis include the downregulation of metabolism, dehydration-induced partitioning of amphiphilic compounds into membranes and immobilization of the cytoplasm in a stable multicomponent glassy matrix. C1 Univ Wageningen & Res Ctr, Grad Sch Expt Plant Sci, Lab Plant Physiol, NL-6703 BD Wageningen, Netherlands. Russian Acad Sci, KA Timiryazev Plant Physiol Inst, Moscow 127276, Russia. Univ Angers, INH, UMR Physiol Mol Semences, INRA,Inst Natl Hort, F-49045 Angers, France. RP Hoekstra, FA, Univ Wageningen & Res Ctr, Grad Sch Expt Plant Sci, Lab Plant Physiol, Arboretumlaan 4, NL-6703 BD Wageningen, Netherlands. TC 113 PD SEP PY 2001 VL 6 IS 9 BP 431 EP 438 UT ISI:000170973900012 ER PT J AU Siepmann, J Peppas, NA TI Modeling of drug release from delivery systems based on hydroxypropyl methylcellulose (HPMC) SO ADVANCED DRUG DELIVERY REVIEWS DE controlled drug delivery; HPMC; hydrophilic matrices; hydroxypropyl methylcellulose; modeling; release mechanism ID DIFFERENTIAL SCANNING CALORIMETRY; SWELLING-CONTROLLED RELEASE; POLYMER DISENTANGLEMENT CONCENTRATION; MATRIX TABLETS; HYDROPHILIC MATRIX; DOSAGE FORMS; CELLULOSE ETHERS; HYDROGEL MATRICES; DIFFUSION LAYER; FORMULATION VARIABLES AB The objective of this article is to review the spectrum of mathematical models that have been developed to describe drug release from hydroxypropyl methylcellulose (HPMC)-based pharmaceutical devices. The major advantages of these models are: (i) the elucidation of the underlying mass transport mechanisms: and (ii) the possibility to predict the effect of the device design parameters (e.g., shape, size and composition of HPMC-based matrix tablets) on the resulting drug release rate, thus facilitating the development of new pharmaceutical products. Simple empirical or semi-empirical models such as the classical Higuchi equation and the so-called power law, as well as more complex mechanistic theories that consider diffusion, swelling and dissolution processes simultaneously are presented, and their advantages and limitations are discussed. Various examples of practical applications to experimental drug release data are given. The choice of the appropriate mathematical model when developing new pharmaceutical products or elucidating drug release mechanisms strongly depends on the desired or required predictive ability and accuracy of the model. In many cases, the use of a simple empirical or semi-empirical model is fully sufficient. However, when reliable, detailed information are required, more complex, mechanistic theories must be applied. The present article is a comprehensive review of the current state of the art of mathematical modeling drug release from HPMC-based delivery systems and discusses the crucial points of the most important theories. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, Coll Pharm, F-49100 Angers, France. Purdue Univ, Sch Chem Engn, W Lafayette, IN 47907 USA. RP Siepmann, J, Univ Angers, Coll Pharm, 16 Blvd Daviers, F-49100 Angers, France. TC 77 PD JUN 11 PY 2001 VL 48 IS 2-3 BP 139 EP 157 UT ISI:000169091200002 ER PT J AU Cales, P Masliah, C Bernard, B Garnier, P Silvain, C Szostak-Talbodec, N Bronowicki, J Ribard, D Botta-Fridlund, D Hillon, P Besseghir, K Lebrec, D CA French Club Study Portal Hypertens TI Early administration of vapreotide for variceal bleeding in patients with cirrhosis SO NEW ENGLAND JOURNAL OF MEDICINE ID ESOPHAGEAL-VARICES; DOUBLE-BLIND; ENDOSCOPIC SCLEROTHERAPY; PORTAL-HYPERTENSION; CONTROLLED TRIAL; SOMATOSTATIN; OCTREOTIDE; HEMORRHAGE; PREVENTION; METAANALYSIS AB Background: In patients with cirrhosis, pharmacologic or endoscopic treatment may control variceal bleeding. However, the effects of early administration of a somatostatin analogue followed by endoscopic treatment are unknown. Methods: We studied the effects of treatment with vapreotide, a somatostatin analogue, begun before endoscopic treatment in 227 patients with cirrhosis who were hospitalized for acute upper gastrointestinal bleeding. The patients were randomly assigned to receive vapreotide (a 50-mug intravenous bolus followed by an infusion at a rate of 50 mug per hour for five days) or placebo within a mean (+/-SD) of 2.3+/-1.5 hours after admission. The patients received endoscopic treatment a mean of 2.6+/-3.3 hours after the infusion was begun. After the exclusion of 31 patients whose bleeding was not caused by portal hypertension, there were 98 patients in each group. Results: At the time of endoscopy, active bleeding was evident in 28 of 91 patients in the vapreotide group (31 percent), as compared with 43 of 93 patients in the placebo group (46 percent, P = 0.03); in 12 patients endoscopy was either impossible or showed portal hypertensive gastropathy. During the five-day infusion, the primary objective - survival and control of bleeding - was achieved in 65 of 98 patients in the vapreotide group (66 percent) as compared with 49 of 98 patients in the placebo group (50 percent) (P = 0.02). The patients in the vapreotide group received significantly fewer blood transfusions (2.0+/-2.2 vs. 2.8+/-2.8 units, P = 0.04). Overall mortality rates at 42 days were not significantly different in the two groups. Conclusions: In patients with cirrhosis and variceal bleeding, the combination of vapreotide and endoscopic treatment is more effective than endoscopic treatment alone as a method of controlling acute bleeding. However, the use of combination therapy does not affect mortality rates at 42 days. (N Engl J Med 2001;344:23-8.) Copyright (C) 2001 Massachusetts Medical Society. C1 CHU Angers, Serv Hepatogastroenterol, F-49033 Angers 01, France. CHU Nantes, Serv Hepatogastroenterol, F-44035 Nantes, France. CHU Pitie Salpetriere, Serv Hepatogastroenterol, Paris, France. CHU La Reunion, Serv Hepatogastroenterol, La Reunion, France. CHU Poitiers, Serv Hepatogastroenterol, Poitiers, France. CHU Lille, Serv Hepatogastroenterol, F-59037 Lille, France. CHU Nancy, Serv Hepatogastroenterol, Nancy, France. CHU Nimes, Serv Hepatogastroenterol, Nimes, France. CHU Marseilles, Serv Hepatogastroenterol, Marseille, France. CHU Dijon, Serv Hepatogastroenterol, Dijon, France. Univ Angers, Lab Hemodynam Splanchn, Angers, France. Debiopharm, Lausanne, Switzerland. Hop Beaujon, INSERM, U481, Clichy, France. Hop Beaujon, Serv Hepatol, Clichy, France. RP Cales, P, CHU Angers, Serv Hepatogastroenterol, F-49033 Angers 01, France. TC 55 PD JAN 4 PY 2001 VL 344 IS 1 BP 23 EP 28 UT ISI:000166160400004 ER PT J AU Murad, AMA d'Enfert, C Gaillardin, C Tournu, H Tekaia, F Talibi, D Marechal, D Marchais, V Cottin, J Brown, AJP TI Transcript profiling in Candida albicans reveals new cellular functions for the transcriptional repressors CaTup1, CaMig1 and CaNrg1 SO MOLECULAR MICROBIOLOGY ID SACCHAROMYCES-CEREVISIAE; GENE-EXPRESSION; CATABOLITE REPRESSION; YEAST GENOME; VIRULENCE; SSN6-TUP1; DISRUPTION; PROTEIN; TUP1; MIG1 AB The pathogenic fungus, Candida albicans contains homologues of the transcriptional repressors ScTup1, ScMig1 and ScNrg1 found in budding yeast. In Saccharomyces cerevisiae, ScMig1 targets the ScTup1/ScSsn6 complex to the promoters of glucose repressed genes to repress their transcription. ScNrg1 is thought to act in a similar manner at other promoters. We have examined the roles of their homologues in C. albicans by transcript profiling with an array containing 2002 genes, representing about one quarter of the predicted number of open reading frames (ORFs) in C. albicans. The data revealed that CaNrg1 and CaTup1 regulate a different set of C. albicans genes from CaMig1 and CaTup1. This is consistent with the idea that CaMig1 and CaNrg1 target the CaTup1 repressor to specific subsets of C. albicans genes. However, CaMig1 and CaNrg1 repress other C. albicans genes in a CaTup1-independent fashion. The targets of CaMig1 and CaNrg1 repression, and phenotypic analyses of nrg1/nrg1 and mig1/mig1 mutants, indicate that these factors play differential roles in the regulation of metabolism, cellular morphogenesis and stress responses. Hence, the data provide important information both about the modes of action of these transcriptional regulators and their cellular roles. The transcript profiling data are available at http://www.pasteur.fr/recherche/ unites/RIF/transcriptdata/. C1 Univ Aberdeen, Inst Med Sci, Aberdeen AB25 2ZD, Scotland. Inst Pasteur, Unite Microbiol & Environm, CNRS, URA 2172, F-75724 Paris 15, France. INAPG, INRA, UMR 216, CNRS,URA 1925, F-788850 Thiverval Grignon, France. Inst Pasteur, Unite Genet Mol Levures, CNRS, URA 2171, F-75724 Paris 15, France. Eurgentec, B-4102 Seraing, Belgium. Univ Angers, Fac Pharm, GEIHP, F-49045 Angers 01, France. RP Brown, AJP, Univ Aberdeen, Inst Med Sci, Aberdeen AB25 2ZD, Scotland. TC 53 PD NOV PY 2001 VL 42 IS 4 BP 981 EP 993 UT ISI:000172341800009 ER PT J AU Hadjadj, S Belloum, R Bouhanick, B Gallois, Y Guilloteau, G Chatellier, G Alhenc-Gelas, F Marre, M TI Prognostic value of angiotensin-1 converting enzyme I/D polymorphism for nephropathy in type 1 diabetes mellitus: A prospective study SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY ID URINARY ALBUMIN EXCRETION; ACE GENE POLYMORPHISM; INSERTION/DELETION POLYMORPHISM; DELETION POLYMORPHISM; CARDIOVASCULAR-DISEASE; RENAL COMPLICATIONS; IGA NEPHROPATHY; BLOOD-PRESSURE; RISK-FACTORS; MICROALBUMINURIA AB Angiotensin-I converting enzyme (ACE) regulates renal hemodynamics. Its insertion/deletion (I/D) polymorphism, which determines most of ACE interindividual variance, was proposed as a genetic marker for diabetic nephropathy. A substitution (M235T) polymorphism in angiotensinogen (AGT) may interact with ACE I/D polymorphism for the risk of diabetic nephropathy, but their prognostic values have to be established by follow-up studies. A total of 310 type I diabetes mellitus patients who attended the diabetic clinic in Angers (France) took part in a prospective, observational, follow-up study. Glycohemoglobin, BP, plasma creatinine, and urinary albumin excretion were determined periodically. Nephropathy was classified as absent, incipient (microalbuminuria), established (proteinuria), advanced (plasma creatinine greater than or equal to 150 mu mol/L), and terminal (renal replacement therapy). The main end point was the occurrence of a renal event defined as the progression to a higher stage of diabetic nephropathy. At baseline, 251 (81%) patients had no nephropathy, 35 (11%) had incipient nephropathy, 18 (6%) had established nephropathy, and 6 (2%) had advanced nephropathy. The ACE I/D and M235T AGT polymorphisms were in Hardy-Weinberg equilibrium in the patients. The median duration of follow-up was 6 yr (range, 2 to 9 yr). The occurrence of renal events was significantly influenced by ACE genotype (log-rank II versus ID versus DD, P < 0.03) with a dominant deleterious effect of the D allele: ID or DD versus II (adjusted hazard ratio, 5.0; 95% confidence interval, 1.5 to 16.6). Other contributors were high glycohemoglobin and systolic BP. In the patients who initially were free of nephropathy, baseline plasma ACE concentration was higher in patients who progressed to microalbuminuria (571 +/- 231 versus 466 +/- 181 g/L; P = 0.0032); the D allele independently favored the occurrence of incipient nephropathy (adjusted hazard ratio, 4.5; 95% confidence interval, 1.1 to 19.4); other contributors were male gender, baseline systolic BP, and urinary albumin excretion. The AGT M235T polymorphism was not associated with renal events. The D allele of the ACE VD polymorphism is an independent risk factor for both the onset and the progression of diabetic nephropathy in type 1 diabetes mellitus patients. C1 Hop Bichat, F-75877 Paris 18, France. CHU Angers, Lab Biochim B, Angers, France. Hop Broussais, Serv Informat Med, F-75674 Paris, France. INSERM, U367, Paris, France. RP Marre, M, Hop Bichat, 46 Rue Henri Huchard, F-75877 Paris 18, France. TC 52 PD MAR PY 2001 VL 12 IS 3 BP 541 EP 549 UT ISI:000167221400015 ER PT J AU Guillausseau, PJ Massin, P Dubois-LaForgue, D Timsit, J Virally, M Gin, H Bertin, E Blickle, JF Bouhanick, B Cahen, J Caillat-Zucman, S Charpentier, G Chedin, P Derrien, C Ducluzeau, PH Grimaldi, A Guerci, B Kaloustian, E Murat, A Olivier, F Paques, M Paquis-Flucklinger, V Porokhov, B Samuel-Lajeunesse, J Vialettes, B TI Maternally inherited diabetes and deafness: A multicenter study SO ANNALS OF INTERNAL MEDICINE ID MITOCHONDRIAL TRNA(LEU(UUR)) GENE; MACULAR PATTERN DYSTROPHY; POINT MUTATION; INSULIN SENSITIVITY; RETINAL DYSTROPHY; TRANSFER-RNA; A-MUTATION; MELLITUS; PREVALENCE; DNA AB Background: Maternally inherited diabetes and deafness (MIDD), which is seen in 0.5% to 2.8% of patients with type 2 diabetes mellitus, is related to a point mutation at position 3243 of mitochondrial (mt) DNA. Its clinical description is incomplete. Objective: To study the clinical presentation and complications of diabetes in patients with MIDD and to identify clinical characteristics that may help select diabetic patients for mtDNA mutation screening. Design: Multicenter prospective descriptive study. Setting: 16 French departments of internal medicine, diabetes and metabolic diseases, or both, Patients: 54 patients with type 2 diabetes mellitus and the mtDNA 3243 mutation. Measurements: Characteristics of diabetes, metabolic control (glycosylated hemoglobin level), complications of diabetes, and Involvement of other organs. Results: On average, patients with MIDD were young at diabetes onset and presented with a normal or low body mass index. None were obese. Seventy-three percent of probands had a maternal family history of diabetes. Diabetes was non-insulin-dependent at onset in 87% of patients; however, 46% of patients had noninsulin-dependent disease at onset but progressed to insulin therapy after a mean duration of approximately 10 years. Neurosensory hearing loss was present in almost all patients. Eighty-six percent of patients who received an ophthalmologic examination had macular pattern dystrophy (a specific retinal lesion), Forty-three percent of patients had myopathy, 15% had cardiomyopathy, and 18% (9 of 51) had neuropsychiatric symptoms. Although the prevalence of diabetic retinopathy was 8% among patients who received an ophthalmologic examination, lower than expected after a mean 12-year duration of diabetes, prevalence of kidney disease was 28%. This suggests that a specific renal involvement was the result of mitochondrial disease. Conclusions: Maternally inherited diabetes and deafness has a specific clinical profile that may help identify diabetic patients for mtDNA testing. C1 Hop Lariboisiere, F-75010 Paris, France. Necker Hosp, Paris, France. Hop La Pitie Salpetriere, Paris, France. Univ Hosp, Pessac, France. Reims Univ Hosp, Hop Robert Debre, Strasbourg, France. Univ Hosp, Angers, France. Victor Dupouy Hosp, Argenteuil, France. Gilles de Corbeil Hosp, Corbeil, France. Univ Hosp, Rennes, France. Hop Edouard Herriot, Lyon, France. Jeanne dArc Hosp, Dommartin Les Toul, France. Hosp Compiegne, Compiegne, France. Hop Hotel Dieu, Nantes, France. Hosp Cahors, Cahors, France. CNRS, UMR 6549, F-06034 Nice, France. Hop St Marguerite, Marseille, France. RP Guillausseau, PJ, Hop Lariboisiere, 2 Rue Ambroise Pare, F-75010 Paris, France. TC 48 PD MAY 1 PY 2001 VL 134 IS 9 BP 721 EP 728 UT ISI:000168470700001 ER PT J AU Le Derf, F Levillain, E Trippe, G Gorgues, A Salle, M Sebastian, RM Caminade, AM Majoral, JP TI Immobilization of redox-active ligands on an electrode: The dendrimer route SO ANGEWANDTE CHEMIE-INTERNATIONAL EDITION ID TETRATHIAFULVALENE DERIVATIVES; FERROCENYL DENDRIMERS; CATION RECOGNITION; ELECTROCHEMISTRY; FILMS C1 Univ Angers, CNRS UMR 6501, Lab Ingn Mol & Mat Organ, F-49045 Angers, France. CNRS, UPR 8241, Chim Coordinat Lab, F-31077 Toulouse 04, France. RP Salle, M, Univ Angers, CNRS UMR 6501, Lab Ingn Mol & Mat Organ, 2 Bd Lavoisier, F-49045 Angers, France. TC 48 PY 2001 VL 40 IS 1 BP 224 EP 227 UT ISI:000166361100030 ER PT J AU Moutquin, JM Cabrol, D Fisk, NM MacLennan, AH Marsal, K Rabinovici, J CA Worldwide Atosiban versus Beta-a TI Effectiveness and safety of the oxytocin antagonist atosiban versus beta-adrenergic agonists in the treatment of preterm labour SO BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY ID CONTROLLED TRIALS; BIRTH-WEIGHT; MORTALITY; MORBIDITY; PARTURITION; RECEPTORS; PREGNANCY; DELIVERY; INFANTS; CARE AB Objective To compare the effectiveness and safety of the oxytocin antagonist atosiban with conventional beta-adrenergic agonist (beta-agonist) therapy in the treatment of preterm labour. Design Three multinational, multicentre, double-blind, randomised, controlled trials. Setting Hospitals in Australia, Canada, Czech Republic, Denmark, France, Israel, Sweden, and the UK. Population Women diagnosed with preterm labour at 23-33 completed weeks of gestation. Methods Seven hundred and forty-two women were randomised; 733 received atosiban (n = 363; intravenous (iv) bolus dose of 6.75 mg, then 300 mug/minute iv. for 3h and 100 mug/min iv thereafter) or beta-agonist (n 379; ritodrine, salbutamol or terbutaline iv; dose titrated) for at least 18h and rip to 48 hours. Uterine contraction rate, cervical dilatation and effacement were used to assess progression of labour. An all patients treated analysis, using the Cochran-Mantel-Haenszel test, was performed. Main outcome measures Tocolytic effectiveness was assessed in terms of the number of women undelivered after 48 hours and seven days. Safety was assessed in terms of maternal side effects and neonatal morbidity. Results There were no significant differences between atosiban and beta -agonists in delaying delivery for 48h (88.1% vs 88.9%; P = 0.99) or seven days (79.7% versus 77.6%; P = 0.28). Tocolytic effectiveness was also similar in terms of mean [SD] gestational age at delivery (35.8 [3.9] weeks vs 35.5 [4.1] weeks) and mean [SD] birthweight (2491 [813] g versus 2461 [831] g). Maternal side effects, particularly cardiovascular adverse events (8.3% vs 81.2%, P < 0.001) were reported more frequently in women given beta -agonists, resulting in more treatment discontinuations due to side effects (1.1% vs 15.4%, P = 0.0001). No statistical differences in neonatal/infant outcomes were observed with either study medication. Conclusions In the largest study of tocolytic therapy to date, atosiban was comparable in clinical effectiveness to conventional beta-agonist therapy, but was associated with fewer maternal cardiovascular side effects. We conclude that atosiban has clinical advantages over current tocolytic therapy. C1 St Francois Asisise Hosp, Quebec City, PQ, Canada. BC Womens Hosp, Vancouver, BC, Canada. Grace Matern Hosp, Halifax, NS, Canada. McMaster Univ, Med Ctr, Hamilton, ON, Canada. St Justine Hosp, Montreal, PQ, Canada. Womens Coll Hosp, Toronto, ON M5S 1B2, Canada. Chaim Sheba Med Ctr, IL-52621 Tel Hashomer, Israel. Assaf Harofeh Med Ctr, Beer Yaakov, Israel. Hadassah Med Org, Jerusalem, Israel. Haemek Med Ctr, Afula, Israel. Meir Med Ctr, Kfar Saba, Israel. Rambam Med Ctr, Haifa, Israel. Soroka Univ Hosp, Beer Sheva, Israel. Univ Hosp, Malmo, Sweden. Akad Hosp, Uppsala, Sweden. Danderyd Hosp, S-18288 Danderyd, Sweden. Karolinska Hosp, S-10401 Stockholm, Sweden. Sahlgrenska Hosp, Gothenburg, Sweden. Sodersjukhuset Hosp, Stockholm, Sweden. Univ Hosp, Lund, Sweden. Ostra Hosp, S-41685 Gothenburg, Sweden. Ferring Pharmaceut, Malmo, Sweden. Univ Copenhagen, Hvidovre Hosp, DK-2650 Hvidovre, Denmark. Rigshosp, DK-2100 Copenhagen, Denmark. Charles Univ, Clin Obstet & Gynecol 1, Prague, Czech Republic. Charles Univ, Clin Obstet & Gynecol 2, Prague, Czech Republic. Clin Obstet & Gynecol, Olomouc, Czech Republic. Clin Obstet & Gynecol, Plzen, Czech Republic. Masaryk Univ, Clin Obstet & Gynecol 1, Brno, Czech Republic. Masaryk Univ, Clin Obstet & Gynecol 2, Brno, Czech Republic. Queen Charlottes & Chelsea Hosp, London W6 0XG, England. Bradford Royal Infirm, Matern Hosp, Bradford BD9 6RJ, W Yorkshire, England. Chelsea & Westminster Hosp, London, England. Ealing Hosp, Southall, Middx, England. Hammersmith Hosp, London, England. Leeds Gen Infirmary, Leeds, W Yorkshire, England. Northwick Pk Hosp, Harrow HA1 3UJ, Middx, England. Royal Infirm, Glasgow G31 2ER, Lanark, Scotland. St James Univ Hosp, Leeds LS9 7TF, W Yorkshire, England. Queen Mothers Hosp, Glasgow, Lanark, Scotland. Univ Edinburgh, Edinburgh, Midlothian, Scotland. W Middlesex Univ Hosp, Isleworth, England. Matern Port Royal, Paris, France. Archet II Hosp, Nice, France. Bichat Hosp, Paris, France. Bocage Hosp, Dijon, France. Bocage Hosp, Tours, France. Ctr Hosp Intercommunal, Creteil, France. Ctr Med Chirurg Schiltigheim, Strasbourg, France. Ctr Med Chirurg Le Parc, Colmar, France. Hop Charles Nicolle, Rouen, France. CHG, Longjumeau, France. CHR Pellegrin Tripode Hosp, Bordeaux, France. CHR Sud Hosp, Rennes, France. CHRU, Caremeau Hosp, Nimes, France. CHRU, Matern Arnaud Villeneuve, Montpellier, France. Cite Hosp Miletrie, Poitiers, France. Civil Hosp, Strasbourg, France. Hasenrein Hosp, Mulhouse, France. Hop Hotel Dieu, Angers, France. Hotel Dieu Matern Hosp, Clermont Ferrand, France. Hotel Dieu Polyclin Hosp, Clermont Ferrand, France. Jean Rostnad Hosp, Sevres, France. Jeanne Flandre Hosp, Lille, France. Hop Louis Mourier, F-92701 Colombes, France. Matern Reg Adolphe Pinard, Nancy, France. Mere Enfant Hosp, Nantes, France. Nord Hosp, Marseille, France. Hop Notre Dame de Bon Secours, Metz, France. Orleans Hosp, Orleans, France. Hop Robert Debre, F-75019 Paris, France. Hop St Antoine, F-75571 Paris, France. Univ Hosp Dupuytren, Limoges, France. Univ Adelaide, Adelaide, SA, Australia. Womens & Childrens Hosp, Adelaide, SA, Australia. King Edward Mem Hosp Women, Subiaco, WA 6008, Australia. King George V Mem Hosp, Camperdown, NSW 2050, Australia. Mater Mothers Hosp, S Brisbane, Qld, Australia. Nepean Hosp, Kingswood, NSW, Australia. Royal Hosp Women, Carlton, Vic, Australia. RP Moutquin, JM, CUSE, Dept Obstet Gynecol, Porte 3143,Site Fleurimont,3001,12e Ave Nord, Sherbrooke, PQ J1H 5N4, Canada. TC 45 PD FEB PY 2001 VL 108 IS 2 BP 133 EP 142 UT ISI:000168649500001 ER PT J AU Lefevre, C Jobard, F Caux, F Bouadjar, B Karaduman, A Heilig, R Lakhdar, H Wollenberg, A Verret, JL Weissenbach, J Ozguc, M Lathrop, M Prud'homme, JF Fischer, J TI Mutations in CGI-58, the gene encoding a new protein of the esterase/lipase/thioesterase subfamily, in Chanarin-Dorfman syndrome SO AMERICAN JOURNAL OF HUMAN GENETICS ID LIPID STORAGE DISEASE; LAMELLAR ICHTHYOSIS; CHILD SYNDROME; FIBROBLASTS; METABOLISM; TRIACYLGLYCEROL; DEHYDROGENASE; DISORDER; LIPASES; LINKAGE AB Chanarin-Dorfman syndrome (CDS) is a rare autosomal recessive form of nonbullous congenital ichthyosiform erythroderma (NCIE) that is characterized by the presence of intracellular lipid droplets in most tissues. We previously localized a gene for a subset of NCIE to chromosome 3 (designated "the NCIE2 locus"), in six families. Lipid droplets were found in five of these six families, suggesting a diagnosis of CDS. Four additional families selected on the basis of a confirmed diagnosis of CDS also showed linkage to the NCIE2 locus. Linkage-disequilibrium analysis of these families, all from the Mediterranean basin, allowed us to refine the NCIE2 locus to an similar to1.3-Mb region. Candidate genes from the interval were screened, and eight distinct mutations in the recently identified CGI-58 gene were found in 13 patients from these nine families. The spectrum of gene variants included insertion, deletion, splice-site, and point mutations. The CGI-58 protein belongs to a large family of proteins characterized by an alpha/beta hydrolase fold. CGI-58 contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. Interestingly, CGI-58 differs from other members of the esterase/lipase/thioesterase subfamily in that its putative catalytic triad contains an asparagine in place of the usual serine residue. C1 Ctr Natl Genotypage, F-91057 Evry, France. CNRS, UMR 8030, F-91057 Evry, France. Univ Paris 13, Unite Propre Rech Enseignement Superieur Equipe A, Immunol Lab, Bobigny, France. Univ Paris 13, Dept Dermatol, Bobigny, France. Bab El Oued Hosp, Dept Dermatol, Algiers, Algeria. Hacettepe Univ, DNA Cell Bank Tubiak, Ankara, Turkey. Hacettepe Univ, Dept Dermatol, Ankara, Turkey. Ibnou Rochd, Dept Dermatol, Casablanca, Morocco. Univ Munich, Dept Dermatol, D-8000 Munich, Germany. CHU Angers, Dept Dermatol, Angers, France. Genethon, Evry, France. RP Fischer, J, Ctr Natl Genotypage, 2 Rue Gaston Cremieux, F-91057 Evry, France. TC 44 PD NOV PY 2001 VL 69 IS 5 BP 1002 EP 1012 UT ISI:000171413400008 ER PT J AU Siepmann, J Gopferich, A TI Mathematical modeling of bioerodible, polymeric drug delivery systems SO ADVANCED DRUG DELIVERY REVIEWS DE bioerosion; modeling; controlled drug delivery; release mechanism; biodegradation; Monte Carlo simulation ID ACID) MICROSPHERES; RELEASE CHARACTERISTICS; INVIVO DEGRADATION; PLGA MICROSPHERES; MONOMER RELEASE; AQUEOUS-MEDIA; EROSION; MATRICES; DEVICES; POLY(ORTHO-ESTER)S AB The aim of this article is to give an introduction into mathematical modeling approaches of bioerodible controlled drug delivery systems and to present the most important erosion theories reported in the literature. First, important parameters such as degradation and erosion are defined and physicochemical methods for their investigation are briefly presented. Then, phenomenological empirical models as well as models based on diffusion and chemical reaction theory are discussed, Due to the significant chemical and physicochemical differences among individual bioerodible polymers used for controlled drug delivery systems, various mathematical models have been developed to describe the chemical reactions and physical mass transport processes involved in erosion-controlled drug release, Various examples of practical applications of these models to experimental drug release data are given, For those involved in the design and development of biodegradable drug delivery systems this will help to choose the appropriate mathematical model for a specific drug release problem, Important selection criteria such as the desired predictive power and precision, but also the effort required to apply a model to a particular system will be discussed, Furthermore, before models can be used for drug release predictions certain parameters such as drug dissolution or polymer degradation rate constants, have to be known. The number of parameters to be determined significantly differs between the models. The practical benefit of carefully choosing the right model is that effects of composition and device geometry on the drug release kinetics can be predicted which can reduce laborious formulation studies to a minimum, (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Regensburg, Dept Pharmaceut Technol, D-93053 Regensburg, Germany. Univ Angers, Coll Pharm, F-49100 Angers, France. RP Gopferich, A, Univ Regensburg, Dept Pharmaceut Technol, Univ Str 31, D-93053 Regensburg, Germany. TC 44 PD JUN 11 PY 2001 VL 48 IS 2-3 BP 229 EP 247 UT ISI:000169091200007 ER PT J AU Cottier, JP Pasco, A Gallas, S Gabrillargues, J Cognard, C Drouineau, J Brunereau, L Herbreteau, D TI Utility of balloon-assisted Guglielmi detachable coiling in the treatment of 49 cerebral aneurysms: A retrospective, multicenter study SO AMERICAN JOURNAL OF NEURORADIOLOGY ID WIDE-NECKED ANEURYSMS; ENDOVASCULAR TREATMENT; INTRACRANIAL ANEURYSMS; TECHNICAL NOTE; SACCULAR ANEURYSMS; OCCLUSION; EMBOLIZATION; ELECTROTHROMBOSIS AB BACKGROUND AND PURPOSE: The management of wide-necked aneurysms or aneurysms with a neck-to-body ratio close to 1 is a difficult challenge for the interventional radiologist because of the risk of coil migration or coil protrusion into the parent vessel. Our objective was to evaluate the efficacy and safety of balloon-assisted coiling as well as the follow-up results of occlusion for those difficult aneurysms in which conventional treatment with Guglielmi detachable coils (GDCs) had failed. METHODS: A nondetachable balloon was used in 49 procedures performed in 44 patients (35 women and nine men) who underwent GDC coiling of aneurysms. Every aneurysm had either a wide neck or a sac diameter/neck size ratio (SNR) of 1.5 or less. RESULTS: In four (8%) of the procedures, balloon placement failed, leaving a total of 45 aneurysms treated with balloon-assisted coiling. Final results consisted of total occlusion in 30 cases (67%), subtotal occlusion in 11 cases (24%), and incomplete occlusion in four cases (9%), We found a correlation between the diameter of the sac and the occlusion rate, but not between the size of the neck or the SNR and the occlusion rate. Two thromboembolic complications occurred, but neither had clinical consequences. No aneurysmal rupture was observed during treatment, Final angiographic follow-up time ranged from 3 months to 5 years (mean, 16 months). CONCLUSION: Balloon-assisted coiling is an important adjunct in the treatment of aneurysms with a wide neck or low SNR, In our experience, this technique allowed safe and efficient treatment of aneurysms when conventional GDC treatment had failed. C1 CHU Bretonneau, Serv Neuroradiol, Dept Neuroradiol, F-37044 Tours, France. CHU Angers, Dept Neuroradiol, Angers, France. Hop Fontmaure, Dept Neuroradiol, Clermont Ferrand, France. CHU Purpan, Dept Neuroradiol, Toulouse, France. CHU Jean Bernard, Dept Neuroradiol, Poitiers, France. RP Cottier, JP, CHU Bretonneau, Serv Neuroradiol, Dept Neuroradiol, 2 Bd Tonnelle, F-37044 Tours, France. TC 44 PD FEB PY 2001 VL 22 IS 2 BP 345 EP 351 UT ISI:000167049300024 ER PT J AU Bienvenu, F Gascan, H Coqueret, O TI Cyclin D1 represses STAT3 activation through a Cdk4-independent mechanism SO JOURNAL OF BIOLOGICAL CHEMISTRY ID CELL-CYCLE; ESTROGEN-RECEPTOR; TRANSCRIPTIONAL ACTIVATION; GENE-EXPRESSION; V-SRC; INHIBITION; KINASE; G1; PHOSPHORYLATION; TRANSFORMATION AB STAT3 transcription factors are cytoplasmic proteins that induce gene activation in response to cytokine receptor stimulation. Following tyrosine phosphorylation, STAT3 proteins dimerize, translocate into the nucleus, and activate specific target genes. Activation is transient, and down-regulation of STAT3 signaling occurs within a few hours. In this study, we show that cyclin D1 inhibits STAT3 activation. In co-immunoprecipitation and pull-down assays, cyclin D1 was found to associate with the activation domain of STAT3 upon interleukin-6 stimulation. Overexpression of cyclin D1 inhibited transcriptional activation by STAT3 proteins. This effect was not shared by cyclin E, was independent of association with Cdk4, and was unaffected by inhibitors of Cdk4, Whereas cyclin D1 had no effect on the steady-state level of STAT3 proteins in the cytoplasm, it was found to reduce the STAT3 nuclear level in HepG2 cells. These results suggest a model by which cyclin D1 is part of a feedback network controlling the down-regulation of STAT3 activity and highlight a new activity for this cell cycle regulatory protein. C1 CHU Angers, INSERM, EMI U 9928, F-49033 Angers, France. RP Coqueret, O, CHU Angers, INSERM, EMI U 9928, 4 Rue Larrey, F-49033 Angers, France. TC 43 PD MAY 18 PY 2001 VL 276 IS 20 BP 16840 EP 16847 UT ISI:000168730400034 ER PT J AU Lelievre, E Plun-Favreau, H Chevalier, S Froger, J Guillet, C Elson, GCA Gauchat, JF Gascan, H TI Signaling pathways recruited by the cardiotrophin-like cytokine/cytokine-like factor-1 composite cytokine - Specific requirement of the membrane-bound form of ciliary neurotrophic factor receptor alpha component SO JOURNAL OF BIOLOGICAL CHEMISTRY ID LEUKEMIA INHIBITORY FACTOR; TYROSINE-PHOSPHATASE SHP-2; ACTIVATED PROTEIN-KINASE; ONCOSTATIN-M; CNTF RECEPTOR; MICE LACKING; TRANSCRIPTION FACTOR; TARGETED DISRUPTION; MOLECULAR-CLONING; TRANSDUCER GP130 AB Ciliary neurotrophic factor (CNTF) is a cytokine sup porting the differentiation and survival of a number of neural cell types. Its receptor complex consists of a ligand-binding component, CNTF receptor (CNTFR), associated with two signaling receptor components, gp130 and leukemia inhibitory factor receptor (LIFR), Striking phenotypic differences between CNTF- and CNTFR-deficient mice suggest that CNTFR serves as a receptor for a second developmentally important ligand, We recently demonstrated that cardiotrophin-like cytokine (CLC) associates with the soluble orphan receptor cytokine-like factor-1 (CLF) to form a heterodimeric cytokine that displayed activities only on cells expressing the tripartite CNTF receptor on their surface. In this present study we examined the membrane binding of the CLC/CLF composite cytokine and observed a preferential interaction of the cytokine with the CNTFR subunit. Signaling pathways recruited by the CLC/CLF complex in human neuroblastoma cell lines were also analyzed in detail. The results obtained showed an activation of Janus kinases (JAK1, JAK2, and TYK2) leading to a tyrosine phosphorylation of the gp130 and LIFR, The phosphorylated signaling receptors served in turn as docking proteins for signal transducing molecules such as STAT3 and SHP-2, In vitro analysis revealed that the gp130-LIFR pathway could also stimulate the phosphatidylinositol 3-kinase and the mitogen-activated protein kinase pathways. In contrast to that reported before for CNTF, soluble CNTFR failed to promote the action CLC/CLF, and an absolute requirement of the membrane form of CNTFR was required to generate a functional response to the composite cytokine. This study reinforces the functional similarity between CNTF and the CLC/CLF composite cytokine defining the second ligand for CNTFR. C1 CHU Angers, INSERM EMI 9928, F-49003 Angers, France. Ctr Immunol Pierre Fabre, F-74164 St Julien En Genevois, France. RP Gascan, H, CHU Angers, INSERM E 9928, 4 Rue Larrey, F-49033 Angers, France. TC 41 PD JUN 22 PY 2001 VL 276 IS 25 BP 22476 EP 22484 UT ISI:000169412700068 ER PT J AU Dahl, SG Allain, P Marie, PJ Mauras, Y Boivin, G Ammann, P Tsouderos, Y Delmas, PD Christiansen, C TI Incorporation and distribution of strontium in bone SO BONE DE strontium; bone formation; mineralization; osteoporosis; gender difference; elimination rate ID POSTMENOPAUSAL OSTEOPOROSIS; CALCIUM-METABOLISM; MINERAL DENSITY; ILIAC BONE; RATS; FLUORIDE; RESORPTION; ARTHRITIS; TURNOVER; SKELETON AB The distribution and incorporation of strontium into bone has been examined in rats, monkeys, and humans after oral administration of strontium (either strontium chloride or strontium ranelate), After repeated administration for a sufficient period of time (at least 4 weeks in rats), strontium incorporation into bone reaches a plateau level, This plateau appears to be lower in females than in males due to a difference in the absorption process. Steady-state plasma strontium levels are reached more rapidly than in bones, and within 10 days in the rat. The strontium levels in bone vary according to the anatomical site. However, strontium Levels at different skeletal sites are strongly correlated, and the strontium content of the lumbar vertebra may be estimated from iliac crest bone biopsies in monkeys. The strontium levels in bone also vary according to the bone structure and higher amounts of strontium are found in cancellous bone than in cortical bone. Furthermore, at the crystal level, higher concentrations of strontium are observed in newly formed bone than in old bone. After withdrawal of treatment, the bone strontium content rapidly decreases in monkeys. The relatively high clearance rate of strontium from bone can be explained by the mechanisms of its incorporation. Strontium is mainly incorporated by exchange onto the crystal surface, In new bone, only a few strontium atoms may be incorporated into the crystal by ionic substitution of calcium. After treatment withdrawal, strontium exchanged onto the crystal is rapidly eliminated, which leads to a rapid decrease in total bone strontium levels, In summary, incorporation of strontium into bone, mainly by exchange onto the crystal surface, is dependent on the duration of treatment, dose, gender, and skeletal site. Nevertheless, bone strontium content is highly correlated with plasma strontium levels and, in bone, between the different skeletal sites. (Bone 28: 446-453; 2001) (C) 2001 by Elsevier Science Inc. All rights reserved. C1 Univ Tromso, Fac Med, Dept Pharmacol, N-9037 Tromso, Norway. CHU Angers, Lab Pharmacol & Toxicol, Angers, France. CNRS, Lariboisiere Hosp, INSERM, U349, Paris, France. Fac Med R Laennec, INSERM, U403, Lyon, France. Univ Geneva, Hop Cantonal, Div Malad Osseuses, Dept Med Interne, CH-1211 Geneva, Switzerland. Inst Rech Int Servier, F-92415 Courbevoie, France. Ctr Clin & Basic Res, Ballerup, Denmark. RP Dahl, SG, Univ Tromso, Fac Med, Dept Pharmacol, N-9037 Tromso, Norway. TC 40 PD APR PY 2001 VL 28 IS 4 BP 446 EP 453 UT ISI:000168496400014 ER PT J AU Niccoli-Sire, P Murat, A Rohmer, V Franc, S Chabrier, G Baldet, L Maes, B Savagner, F Giraud, S Bezieau, S Kottler, ML Morange, S Conte-Devolx, B CA French Calcitonin Tumors Study Grp TI Familial medullary thyroid carcinoma with noncysteine RET mutations: Phenotype-genotype relationship in a large series of patients SO JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM ID MULTIPLE-ENDOCRINE-NEOPLASIA; PROTOONCOGENE POINT MUTATIONS; DE-NOVO MUTATIONS; PROPHYLACTIC THYROIDECTOMY; MEN 2A; CODON-918 MUTATION; DISEASE PHENOTYPE; KINASE DOMAIN; TYPE-2; 2B AB Familial medullary thyroid carcinoma only is related to germ. line mutations in the protooncogene RET, mainly in exons 10, whereas noncysteine mutations (exons 13-15) are considered infrequent. We analyzed 148 patients from 47 familial medullary thyroid carcinoma only families, and we found noncysteine RET mutations in 59.5% of these families. Of the index cases with noncysteine mutations, 43.4% presented with a multinodular goiter and high basal calcitonin; they were older at diagnosis than those with mutation in exon 10 and had more multifocal medullary thyroid carcinoma, but no difference in size, bilaterality, presence of C cell hyperplasia, or nodal metastases was found. Gene carriers with noncysteine RET mutations had a lower incidence of medullary thyroid carcinoma (78.2% vs. 94.1%) than those with mutation in exon 10; 20.2% had C cell hyperplasia only, although thyroidectomized at an older age. In conclusion, familial medullary thyroid carcinoma with noncysteine RET mutations are not infrequent and are overrepresented in presumed sporadic medullary thyroid carcinoma, suggesting that RET analysis should routinely be extended to exons 13,14, and 15. The phenotype is characterized by a late onset of the disease, suggesting a delayed appearance of C cell disease rather than a less aggressive form. In familial medullary thyroid carcinoma gene carriers, the optimal timing for thyroidectomy remains controversial. Based on these data, we propose that surgery should be performed before elevation of the basal calcitonin level, potentially as soon as the pentagastrin test becomes abnormal. C1 CHU Timone, Serv Endocrinol, F-13385 Marseille 05, France. CHU Timone, Dept Informat Med, F-13385 Marseille, France. Serv Endocrinol, F-44035 Nantes, France. Genet Mol Lab, F-44035 Nantes, France. CHU Angers, Serv Endocrinol, F-49033 Angers, France. CHU Angers, Biol Mol Lab, F-49033 Angers, France. CHU Avicenne, Serv Endocrinol, F-93009 Bobigny, France. CHU Strasbourg, Serv Med Interne, F-67098 Strasbourg, France. CHU Montpellier, Serv Endocrinol, F-34295 Montpellier, France. Inst Jean Godinot, F-51056 Reims, France. Hop Edouard Herriot, Genet Lab, F-69437 Lyon, France. Hop La Pitie Salpetriere, Serv Biochim Med, Unite Genet Mol, F-75651 Paris, France. RP Niccoli-Sire, P, CHU Timone, Serv Endocrinol, 254 Rue St Pierre F, F-13385 Marseille 05, France. TC 37 PD AUG PY 2001 VL 86 IS 8 BP 3746 EP 3753 UT ISI:000170430200042 ER PT J AU Reynier, P May-Panloup, P Chretien, MF Morgan, CJ Jean, M Savagner, F Barriere, P Malthiery, Y TI Mitochondrial DNA content affects the fertilizability of human oocytes SO MOLECULAR HUMAN REPRODUCTION DE cytoplasmic maturation; mitochondrial biogenesis; mtDNA; oocyte; real-time PCR ID BOVINE OOCYTES; IN-VITRO; BOTTLENECK; FERTILIZATION; SPERMATOZOA; MATURATION; DELETIONS; MOTILITY; EMBRYOS; NUMBER AB Mitochondrial DNA content varies considerably in oocytes, even when collected from the same patient. In the present study, real-time quantitative polymerase chain reaction analysis of 113 unfertilized oocytes obtained from 43 patients revealed an average of 193 000 (range: 20 000 to 598 000) mitochondrial genomes per cell, We compared several groups of oocytes to investigate the relationship between mitochondrial DNA content and fertilizability, The average mitochondrial DNA copy number was significantly lower in cohorts suffering from fertilization failure compared to cohorts with a normal rate of fertilization. In addition, the mitochondrial copy number of oocytes from patients with fertilization failure due to unknown causes was significantly lower than that of oocytes from patients in which IVF failure was due mainly to a severe sperm defect. The lower mtDNA copy number could be due to defective cytoplasmic maturation of oocytes. We conclude that low mitochondrial DNA content, due to inadequate mitochondrial biogenesis or cytoplasmic maturation, may adversely affect oocyte fertilizability. C1 CHU Angers, INSERM, Lab Biochim & Biol Mol, F-49033 Angers, France. CHU Angers, Lab Histol Embryol Cytol, F-49033 Angers, France. CHU Angers, Lab FIV, F-49033 Angers, France. CHU Nantes, F-44093 Nantes 1, France. RP Reynier, P, CHU Angers, INSERM, Lab Biochim & Biol Mol, EMI-U 00-18, F-49033 Angers, France. TC 37 PD MAY PY 2001 VL 7 IS 5 BP 425 EP 429 UT ISI:000168678300004 ER PT J AU Portet, D Denizot, B Rump, E Lejeune, JJ Jallet, P TI Nonpolymeric coatings of iron oxide colloids for biological use as magnetic resonance imaging contrast agents SO JOURNAL OF COLLOID AND INTERFACE SCIENCE DE nanoparticle; iron oxide; bisphosphonate; diphosphonate; quaternary ammonium; phosphorylcholine; adsorption; monolayer ID PHOSPHORYLCHOLINE; NANOPARTICLES AB Iron oxide nanoparticles are used in vivo as contrast agents in magnetic resonance imaging. Their widely used polymer coatings are directly involved in their biocompatibility and avoid magnetic aggregation. As these polymer brushes also limit their tissular diffusion due to important hydrodynamic sizes, this work looks to obtain particles coated with thin layers of organic biocompatible molecules. Coating molecules were chosen depending on their fixation site on iron cores; carboxylates, sulfonates, phosphates, and phosphonates, and, among them, analogs of the phosphorylcholine. Two coating procedures (dialysis and exchange resins purification) were evaluated for hydrodynamic size, total iron concentration, electrophoretic mobility, and colloidal stability. Furthermore, a complementary test on stainless steel plates evaluated the contamination by competition of phosphonates as a rough estimation of the biocompatibility of the particles. Coating with bisphosphonates, the more interesting fixation moiety, leads to small (less than 15 nm) and stable objects in a wide range of pH including the neutrality. From stability data, the coating density was evaluated at around 1.6 molecules per nm(2). Including a quaternary ammonium salt to the coating molecule lowers their electrophoretic mobility. Moreover, this type of coating protects steel plates against contamination without significant desorption. Ah these properties allow further developments of these nanoparticles for biomedical applications. (C) 2001 Academic Press. C1 Univ Angers, Fac Med, UPRES, EA 2169 Vectorisat Particulaire, F-49045 Angers, France. Aventis, F-69009 Lyon, France. RP Portet, D, Univ Angers, Fac Med, UPRES, EA 2169 Vectorisat Particulaire, 1 Rue Haute Reculee, F-49045 Angers, France. TC 35 PD JUN 1 PY 2001 VL 238 IS 1 BP 37 EP 42 UT ISI:000168908800006 ER PT J AU Le Derf, F Mazari, M Mercier, N Levillain, E Trippe, G Riou, A Richomme, P Becher, J Garin, J Orduna, J Gallego-Planas, N Gorgues, A Salle, M TI Tetrathiafulvalene crowns: Redox-switchable ligands SO CHEMISTRY-A EUROPEAN JOURNAL DE crown compounds; cyclic voltammetry; O ligands; solid-state structures; tetrathiafulvalenes ID TRANS-CIS ISOMERIZATION; ANNELATED TETRATHIAFULVALENES; METAL COMPLEXATION; DERIVATIVES; SUPERCONDUCTORS; RECOGNITION; SALTS AB A series of redox-responsive ligands that associate the electroactive tetrathiafulvalene core with polyether subunits of various lengths has been synthesized. X-ray structures are provided for each of the free ligands, The requisite structural criteria for reaching switchable ligands are satisfied for the largest macrocycles, that is, planarity of the 1,1',3,3'-tetrathiafulvalene (TTF) pi system and correctly oriented coordinating atoms. The ability of these ligands to recognize various metal cations as a function of the cavity size has been investigated by various techniques (LSIMS,H-1 NMR, and UV/Vis spectroscopy, cyclic voltammetry). These systems exhibit an unprecedented high coordination ability among TTF crown ethers. Their switchable ligating properties have been confirmed by cyclic voltammetry, and metal-cation complexation has been illustrated by X-ray structures of three of the corresponding metal complexes (Pb2+, Sr2+, and Ba2+). Solid-stale structures of these complexes display original packing modes with channel-like arrangements. C1 Univ Angers, UMR CNRS 6501, Lab Ingn Mol & Mat Organ, F-49045 Angers, France. Univ Angers, SC RMN, F-49045 Angers, France. Odense Univ, Dept Chem, DK-5230 Odense, Denmark. Univ Zaragoza, CSIC, Fac Ciencias,Inst Ciencia Mat Aragon, Unidad Nuevos Mat Organicos, E-50009 Zaragoza, Spain. Univ Oran Es Senia, Lab Synth Organ Appliquee, Oran, Algeria. RP Salle, M, Univ Angers, UMR CNRS 6501, Lab Ingn Mol & Mat Organ, 2 Blvd Lavoisier, F-49045 Angers, France. TC 35 PD JAN 19 PY 2001 VL 7 IS 2 BP 447 EP 455 UT ISI:000166762000015 ER PT J AU Plun-Favreau, H Elson, G Chabbert, M Froger, J deLapeyriere, O Lelievre, E Guillet, C Hermann, J Gauchat, JF Gascan, H Chevalier, S TI The ciliary neurotrophic factor receptor alpha component induces the secretion of and is required for functional responses to cardiotrophin-like cytokine SO EMBO JOURNAL DE cardiotrophin-like cytokine; CNTF receptor; gp130; LIF receptor; motor neuron ID LEUKEMIA INHIBITORY FACTOR; AMYOTROPHIC-LATERAL-SCLEROSIS; IL-6 SIGNAL TRANSDUCER; CNTF RECEPTOR; CRYSTAL-STRUCTURE; ONCOSTATIN-M; ERYTHROPOIETIN RECEPTOR; INTERLEUKIN-6 FAMILY; AFFINITY RECEPTORS; MOLECULAR-CLONING AB Ciliary neurotrophic factor (CNTF) is involved in the survival of a number of different neural cell types, including motor neurons. CNTF functional responses are mediated through a triparaite membrane receptor composed of two signalling receptor chains, gp130 and the leukaemia inhibitory factor receptor (LIFR), associated with a non-signalling CNTF binding receptor a component (CNTFR). CNTFR-deficient mice show profound neuronal deficits at birth, leading to a lethal phenotype, In contrast, inactivation of the CNTF gene leads only to a slight muscle weakness, mainly during adulthood, suggesting that CNTFR binds to a second ligand that is important for development. Modelling studies of the interleukin-6 family member cardiotrophin-like cytokine (CLC) revealed structural similarities with CNTF, including the conservation of a site I domain involved in binding to CNTFR, Coexpression of CEC and CNTFR in mammalian cells generates a secreted composite cytokine, displaying activities on cells expressing the gp130-LIFR complex on their surface, Correspondingly, CLC-CNTPR activates gp130, LIFR and STAT3 signalling components, and enhances motor neuron survival. Together, these observations demonstrate that CNTFR induces the secretion of CLC, as well as mediating the functional responses of CLC. C1 CHU Angers, INSERM, F-49033 Angers, France. Ctr Immunol Pierre Fabre, F-74164 St Julien En Genevois, France. Univ Mediterranee, CNRS, INSERM, U382,IBDM, F-13288 Marseille, France. RP Gascan, H, CHU Angers, INSERM, EMI 9928,4 Rue Larrey, F-49033 Angers, France. TC 31 PD APR 2 PY 2001 VL 20 IS 7 BP 1692 EP 1703 UT ISI:000167981400021 ER PT J AU Brun, LA Maillet, J Hinsinger, P Pepin, M TI Evaluation of copper availability to plants in copper-contaminated vineyard soils SO ENVIRONMENTAL POLLUTION DE copper; contamination; bioavailbility; vineyard; Bordeaux mixture ID METAL; EXTRACTANTS; SUITABILITY; TOXICITY; CADMIUM; CATIONS; CITRUS; SLUDGE; BORNE; ZINC AB The repeated use of copper (Cu) fungicides to control vine downy mildew has led to long-term accumulation of Cu in vineyard soils which now raises the issue of the potential bioavailability of Cu for various living organisms including plant species. The bioavailable Cu can be defined as the portion of soil Cu that can be taken up by roots, for a given plant species. In order to evaluate the bioavailability of Cu to plants, a pot experiment was conducted in glasshouse conditions with a crop species (maize) and 12 soils sampled in the upper horizon of 10 vineyard plots (total Cu ranging from 38 to 251 mg kg(-1)) and two woodland plots (control soils that had not received any Cu application; total Cu amounting to 20-26 mg kg(-1)). These soils were selected for their diverse physical (large range of particle size distribution) and chemical (from acid to calcareous soils) properties. After 35 days of growth, plant shoots were harvested for analysis. The roots were separated from soil particles for further analysis. The concentrations of Cu in the roots and aerial parts of the maize were then compared with the amounts of Cu extracted from the soil by a range of conventional extractants. Observed Cu concentrations in maize roots which have grown in contaminated vineyard soils were very high (between 90 and 600 mg kg(-1)), whereas Cu concentrations in the aerial parts varied only slightly and remained low(< 18 mg kg(-1)). Root Cu concentrations observed for maize increased with increasing total Cu content in the soil and with decreasing soil CEC. Cu accumulation in maize roots may be as high in calcareous soils as in acid soils, suggesting that soil pH had little influence. In the case of the Vineyard soils studied, the lack of correlation found for maize between Cu concentrations in roots and in the aerial parts, suggests that an analysis of the aerial parts would not be a good indicator of plant Cu uptake, as it provides no insight into the real amount of Cu transferred from the soil to the plant. For maize, our results show that extraction with organic complexing agents (EDTA, DTPA) and extraction with ammonium acetate seem to provide a reasonably good estimate of root Cu concentration. (C) 2000 Elsevier Science Ltd. All rights reserved. C1 Ecole Natl Super Agron Montpellier, INRA, Dept Plant Biol Ecol & Pathol, F-34060 Montpellier 1, France. INRA, UMR Sci Sol, F-34060 Montpellier, France. RP Brun, LA, INH, Unite Protect Plantes, 2 Rue Le Notre, F-49045 Angers 01, France. TC 31 PY 2001 VL 111 IS 2 BP 293 EP 302 UT ISI:000089978700013 ER PT J AU Huang, W Gallois, Y Bouby, N Bruneval, P Heudes, D Belair, MF Krege, JH Meneton, P Marre, M Smithies, O Alhenc-Gelas, F TI Genetically increased angiotensin I-converting enzyme level and renal complications in the diabetic mouse SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA ID HUMAN-GENOME; DELETION POLYMORPHISM; GENE; NEPHROPATHY; MELLITUS; DISEASE; MICROALBUMINURIA; INHIBITION; PREVENTION; PLASMA AB Diabetic nephropathy is a major risk factor for end-stage renal disease and cardiovascular diseases and has a marked genetic component. A common variant (D allele) of the angiotensin I-converting enzyme (ACE) gene, determining higher enzyme levels, has been associated with diabetic nephropathy. To address causality underlying this association, we induced diabetes in mice having one, two, or three copies of the gene, normal blood pressure, and an enzyme level range (65-162% of wild type) comparable to that seen in humans. Twelve weeks later, the three-copy diabetic mice had increased blood pressures and overt proteinuria. Proteinuria was correlated to plasma ACE level in the three-copy diabetic mice. Thus, a modest genetic increase in ACE levels is sufficient to cause nephropathy in diabetic mice. C1 INSERM Unit 367, F-75005 Paris, France. CHU Angers, Dept Biochem, F-44033 Angers, France. INSERM Unit 430, F-75014 Paris, France. Univ N Carolina, Dept Pathol & Lab Med, Chapel Hill, NC 27599 USA. CHU Xavier Bichat, Diabetol Dept, F-75008 Paris, France. RP Alhenc-Gelas, F, INSERM Unit 367, 17 Rue Fer Moulin, F-75005 Paris, France. TC 30 PD NOV 6 PY 2001 VL 98 IS 23 BP 13330 EP 13334 UT ISI:000172076800089 ER PT J AU Becouarn, Y Gamelin, E Coudert, B Negrier, S Pierga, JY Raoul, JL Provencal, J Rixe, O Krisch, C Germa, C Bekradda, M Mignard, D Mousseau, M TI Randomized multicenter phase II study comparing a combination of fluorouracil and folinic acid and alternating irinotecan and oxaliplatin with oxaliplatin and irinotecan in fluorouracil-pretreated metastatic colorectal cancer patients SO JOURNAL OF CLINICAL ONCOLOGY ID HIGH-DOSE LEUCOVORIN; PLUS SUPPORTIVE CARE; FIRST-LINE TREATMENT; CONTINUOUS-INFUSION; 5-FLUOROURACIL REGIMEN; TRIAL; CHEMOTHERAPY; CPT-11; RESISTANT; BOLUS AB Purpose : To assess antitumor activity and safety of two regimens in advanced colorectal cancer (CRC) patients with proven fluorouracil (5-FU) resistance in a randomized phase II study: 5-FU/folinic acid (FA) combined with alternating irinotecan (also called CPT-11) and oxaliplatin (FC/FO tritherapy), and an oxaliplatin/irinotecan (OC) combination. Patients and Methods: Sixty-two patients were treated: arm FC/FO (32 patients) received, every 4 weeks, FA 200 mg/m(2) followed by a 400-mg/m(2) 5-FU bolus injection, then a 600-mg/m(2) continuous infusion of 5-FU on days 1 and 2 every 2 weeks administered alternately with irinotecan (180 mg/m(2) on day 1) and oxaliplatin (85 mg/m(2) on day 15). Arm OC (30 patients) received oxaliplatin 85 Mg/M2 and irinotecan 200 mg/m(2) every 3 weeks. Results: In an intent-to-treat analysis, two partial responses lasting 10.7 and 16 months were observed with the tritherapy regimen, and seven (median duration, 11 months; range, 10.6 to 11.4 months) were observed with the bitherapy regimen. Median progression-free and overall survival times were 8.2 and 9.8 months, respectively, in the FC/FO arm and 8.5 and 12.3 months, respectively, in the OC arm. Main grade 3/4 toxicities were, respectively, neutropenia, 53% and 47%; febrile neutropenia, 13% and 3%; diarrhea, 19% and 10%; vomiting, 6% and 13%; and neurosensory toxicity, 3% and 3%. No treatment-related deaths occurred. Conclusion: The every-3-weeks OC combination is safe and active in advanced 5-FU-resistant CRC patients. The lower activity data seen with the tritherapy regimen may be related to the lower dose intensities of irinotecan and oxaliplatin in this schedule. (C) 2001 by American Society of Clinical Oncology. C1 Inst Bergonie, Reg Canc Ctr, Dept Digest Oncol, F-33076 Bordeaux, France. Ctr Paul Papin, Angers, France. Ctr Georges Francois Leclerc, Dijon, France. Ctr Leon Berard, F-69373 Lyon, France. Inst Curie, Paris, France. Aventis, Paris, France. Ctr Eugene Marquis, Rennes, France. CHU Grenoble, F-38043 Grenoble, France. Clin C Bernard, Metz, France. Ctr Hosp Univ, Amiens, France. Cvitkovic & Associes Consultants, Le Kremlin Bicetre, France. RP Becouarn, Y, Inst Bergonie, Reg Canc Ctr, Dept Digest Oncol, 229 Cours Argonne, F-33076 Bordeaux, France. TC 29 PD NOV 15 PY 2001 VL 19 IS 22 BP 4195 EP 4201 UT ISI:000172206500002 ER PT J AU Lamprecht, A Ubrich, N Yamamoto, H Schafer, U Takeuchi, H Maincent, P Kawashima, Y Lehr, CM TI Biodegradable nanoparticles for targeted drug delivery in treatment of inflammatory bowel disease SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS ID PHOSPHODIESTERASE INHIBITOR ROLIPRAM; COLON-SPECIFIC DELIVERY; INDUCED ARTHRITIS; PARTICLE-SIZE; RATS; COLITIS; MODELS; TRANSLOCATION; MICROSPHERES; AMELIORATION AB The use of nanoparticles for targeted oral drug delivery to the inflamed gut tissue in inflammatory bowel disease was examined. Such a strategy of local drug delivery would be a distinct improvement compared with existing colon delivery devices for this disease. An experimental colitis was induced by trinitrobenzenesulfonic acid to male Wistar rats. Rolipram, an anti-inflammatory model drug, was incorporated within poly(lacticcoglycolic acid) nanoparticles, which were administered once a day orally for five consecutive days. A clinical activity score and myeloperoxidase activity were determined to assess the inflammation, whereas an adverse effect index reflected the remaining neurotropic effect of rolipram resulting from its systemic absorption. All nanoparticle formulations proved to be as efficient as the drug in solution in mitigating the experimental colitis. The clinical activity score and myeloperoxidase activity decreased significantly after the oral administration of rolipram nanoparticles or solution. During the next 5 days when animals were kept without drug treatment the drug solution group displayed a strong relapse, whereas the nanoparticle groups continued to show reduced inflammation levels. The rolipram solution group had a high adverse effect index, whereas the rolipram nanoparticle groups proved their potential to retain the drug from systemic absorption as evidenced by a significantly reduced index. This new delivery system enabled the drug to accumulate in the inflamed tissue with higher efficiency than when given as solution. The nanoparticle deposition in the inflamed tissue should be given particular consideration in the design of new carrier systems for the treatment of inflammatory bowel disease. C1 Univ Saarland, Dept Biopharmaceut & Pharmaceut Technol, D-6600 Saarbrucken, Germany. Fac Pharm Nancy, Pharm Galen & Biopharm Lab, Nancy, France. Gifu Pharmaceut Univ, Lab Pharmaceut Engn, Gifu, Japan. RP Lamprecht, A, Univ Angers, INSERM, ERITM 0104, 10 Rue Andre Boquel, F-49100 Angers, France. TC 28 PD NOV PY 2001 VL 299 IS 2 BP 775 EP 781 UT ISI:000171764100047 ER PT J AU Grolleau, F Gamelin, L Boisdron-Celle, M Lapied, B Pelhate, M Gamelin, E TI A possible explanation for a neurotoxic effect of the anticancer agent oxaliplatin on neuronal voltage-gated sodium channels SO JOURNAL OF NEUROPHYSIOLOGY ID UNPAIRED MEDIAN NEURONS; CISPLATIN NEUROTOXICITY; COLORECTAL-CANCER; PLATINUM; MECHANISM; TOXICITY AB Oxaliplatin, a new widely used anticancer drug, displays frequent, sometimes severe, acute sensory neurotoxicity accompanied by neuromuscular signs that look like the symptoms observed in tetany and myotonia. The whole cell patch-clamp technique was employed to investigate the oxaliplatin effects on the electrophysiological properties of short-term cultured dorsal unpaired median (DUM) neurons isolated from the CNS of the cockroach Periplaneta americana. Within the clinical concentration range, oxaliplatin (40-500 muM), applied intracellularly, decreased the amplitude of the voltage-gated sodium current resulting in a reduction of half the amplitude of the action potential. For comparison, two other platinum derivatives, cisplatin and carboplatin, were found to be ineffective at reducing the sodium current amplitude. In addition, we compared the oxaliplatin action to those of its metabolites dichlorodiaminocyclohexane platinum (dach-Cl-2-platin) and oxalate. Oxalate (500 muM) was found to be effective, like oxaliplatin, at reducing the inward sodium current amplitude, whereas dach-Cl-2-platin (500 muM) failed to change the current amplitude. Interestingly, the effect of oxalate or oxaliplatin could be mimicked by using intracellularly applied 10 mM bis-(o-aminophenoxy)-N,N,N',N'-tetraacetic acid (BAPTA), known as chelator of calcium ions. We concluded that oxaliplatin was capable of altering the voltage-gated sodium channels through a pathway involving calcium ions probably immobilized by its metabolite oxalate. The medical interest of preventing acute neurotoxic side effects of oxaliplatin by infusing Ca2+ and Mg2+ is discussed. C1 Univ Angers, UFR Sci, UPRES, EA 2647,Lab Neurophysiol, F-49045 Angers, France. CHU Angers, Lab Biol Mol Immunol & Therapeut Canc, F-49033 Angers, France. RP Grolleau, F, Univ Angers, UFR Sci, UPRES, EA 2647,Lab Neurophysiol, 2 Blvd Lavoisier, F-49045 Angers, France. TC 28 PD MAY PY 2001 VL 85 IS 5 BP 2293 EP 2297 UT ISI:000168675100046 ER PT J AU Colomb, E Nguyen, TD Bechetoille, A Dascotte, JC Valtot, F Brezin, AP Berkani, M Copin, B Gomez, L Polansky, JR Garchon, HJ TI Association of a single nucleotide polymorphism in the TIGR/MYOCILIN gene promoter with the severity of primary open-angle glaucoma SO CLINICAL GENETICS DE genetics; glaucoma; myocilin; ocular hypertension; promoter; single nucleotide polymorphism ID GLUCOCORTICOID RESPONSE PROTEIN; HORMONE REPLACEMENT THERAPY; TRABECULAR MESHWORK; INTRAOCULAR-PRESSURE; OCULAR HYPERTENSION; MUTATIONS; IDENTIFICATION; LOCALIZATION; TIGR; RISK AB Primary open-angle glaucoma (POAG) is a highly prevalent optic neuropathy and a major cause of irreversible blindness, with elevation of intraocular pressure (IOP) being a primary risk factor. The trabecular meshwork-inducible glucocorticoid response (TIGR)/MYOCILIN (MYOC) gene coding region is mutated in 3-4% of POAG patients. Here, in a retrospective study of 142 POAG patients, we evaluated the influence on glaucoma phenotype of a novel biallelic polymorphism ( -1000C/G) located in the upstream region of the MYOC gene. Allele frequencies were similar among patients and controls. However, the G allele (frequency 17.6%), also designated as MYOC.mt1, was associated with an increased IOP ( + 4.9 mmHg, p = 0.0004) and a more damaged visual field (p = 0.02). Both effects were predominant in females. Moreover, whereas IOP in MYOC.mt1 noncarriers decreased very markedly to the normal range between diagnosis and inclusion in the study (p 3 x 10(-5) in both males and females), reflecting successful therapy, it decreased less noticeably in MYOC.mt1 + male patients (p = 0.005) and not at all in MYOC.mt1 + female patients. MYOC.mt1 appears therefore to be an indicator of poor IOP control and greater visual field damage in diagnosed POAG patients, potentially due to a lack of response to therapeutic intervention. Its typing might help in the selection of treatment paradigms for the management of POAG patients. C1 Hop St Joseph, Inst Glaucome, F-75674 Paris, France. CHU Cochin Port Royal, Serv Ophtalmol, Paris, France. CHU Lille, Serv Ophtalmol, F-59037 Lille, France. CHU Angers, Serv Ophtalmol, Angers, France. Univ Calif San Francisco, Dept Ophthalmol, San Francisco, CA 94143 USA. INSERM U25, Paris, France. RP Garchon, HJ, Hop Necker Enfants Malad, INSERM U25, 161 Rue Sevres, F-75743 Paris 15, France. TC 27 PD SEP PY 2001 VL 60 IS 3 BP 220 EP 225 UT ISI:000171714600010 ER PT J AU Bernard, M Gressin, R Lefrere, F Drenou, B Branger, B Caulet-Maugendre, S Tass, P Brousse, N Valensi, F Milpied, N Voilat, L Sadoun, A Ghandour, C Hunault, M Leloup, R Mannone, L Hermine, O Lamy, T TI Blastic variant of mantle cell lymphoma: a rare but highly aggressive subtype SO LEUKEMIA DE mantle cell lymphoma; blastic variant; clinical features ID POLYMERASE CHAIN-REACTION; CYCLIN D1; TRANSPLANTATION; GENES; OVEREXPRESSION; P53; CLASSIFICATION; EXPRESSION; MUTATIONS; PROPOSAL AB The blastic variant (BV) form of mantle cell lymphoma (MCL) Is considered to be a very aggressive subtype of non-Hodgkin's lymphoma (NHL). In order to determine Its clinico-biological features and response to therapy we studied 33 patients (17%) out of 187 suffering from MCL who were diagnosed with a BV of MCL. Blastic variant was diagnosed according to histopathological patterns, Immunophenotyping, and bcl1 gene rearrangement and/or cyclin DI overexpression. Three patients Initially diagnosed with large cell NHL were classified as BV. Patients received front-line therapy including CHOP-like regimen or CVP (n = 29), or chlorambucil (n = 4) and CHOP or ESAP as second-line therapy. High-dose Intensification with stem cell transplantation (SCT) was performed in 11 cases (autoSCT, n = 8; alloSCT, n = 3). All but two patients were in complete remission (CR) at the time of transplant (CRI, n = 5; CR2, n = 4). Clinical and biological characteristics did not differ from those of the common form of MCL. The median age was 62 years (29-80), with a sex ratio (MIF) of 2.6:1. Of the 33 patients, 66% had extranodal site Involvement, 85% had an Ann Arbor stage IV, and 82% had peripheral lymphadenopathy. Circulating lymphomatous cells were seen in 48% of cases. Twelve patients (36%) entered a CRI with a median duration of 11 months. Fifteen patients (46%) failed to respond and rapidly died of progressive disease. Second-line therapy led to a 26% (6/23) CR2 rate. Nine patients relapsed after high-dose therapy. Twenty-two of the 33 patients (66%) died of refractory or progressive disease. Median overall survival (OS) time was 14.5 months for the 33 BV patients as compared to 53 months for the 154 patients with a common form of MCL, P < 0.0001. In the univariate analysis, OS was influenced by age, extranodal site involvement, circulating lymphomatous cells, and international prognosis Index (IPI). In the multivariate analysis, only IPI affected OS: patients with IPI greater than or equal to2 had 8 months median OS as compared to 36 months median OS for patients with IPI <2, P = 0.003. Blastic variant is one of the worst forms of NHL. An improved recognition of BV of MCL Is required, particularly in high-grade CD5(+) NHL using Immunophenotyping and bcl1 molecular study. Standard therapy using anthracycline or even high-dose Intensification produce poor results and an alternative treatment should be proposed to such patients. C1 CHU Rennes, Hop Pontchaillou, Serv Hematol Clin, Hematol Lab, F-35033 Rennes, France. CHU Dijon, Serv Hematol, Dijon, France. CHU Orleans, Serv Hematol, Orleans, France. CHU Angers, Serv Hematol, Angers, France. Clin Hematol, Cesson, France. CHU Poitiers, Serv Hematol, Poitiers, France. CHU Besancon, Serv Hematol, F-25030 Besancon, France. CHU Nantes, Serv Hematol, F-44035 Nantes, France. Hop Necker Enfants Malad, Lab Hematol Biol, Paris, France. Hop Necker Enfants Malad, Anat Pathol Lab, Paris, France. CHU Rennes, Anat Pathol Lab, Rennes, France. CHU Rennes, Serv Epidemiol, Rennes, France. Hop Necker Enfants Malad, Serv Hematol Clin, Paris, France. CHU Grenoble, Serv Hematol, F-38043 Grenoble, France. RP Lamy, T, CHU Rennes, Hop Pontchaillou, Serv Hematol Clin, Hematol Lab, F-35033 Rennes, France. TC 26 PD NOV PY 2001 VL 15 IS 11 BP 1785 EP 1791 UT ISI:000171918100018 ER PT J AU Rouleau, F Merheb, M Geffroy, S Berthelot, J Chaleil, D Dupuis, JM Victor, J Geslin, P TI Echocardiographic assessment of the interventricular delay of activation and correlation to the QRS width in dilated cardiomyopathy SO PACE-PACING AND CLINICAL ELECTROPHYSIOLOGY DE dilated cardiomyopathy; ventricular activation; mechanics; echocardiography; Doppler tissue imaging ID STAGE HEART-FAILURE; BUNDLE-BRANCH-BLOCK; CONDUCTION DELAY; PREDICTORS AB The aim of the study was to define criteria for left ventricular pacing in dilated cardiomyopathy (DCM) using an echocardiographic evaluation of interventricular electromechanical delay (IMD) and a correlation of IMD to QRS duration. Standard 12-lead ECG and echocardiography with pulsed Doppler tissue imaging (DTI) were recorded in 35 DCM patients (mean age 58 +/- 11 years) with QRS duration from narrow(80 ms) to broad (222 ms) patterns. The time for left ventricular activation was evaluated from the onset of QRS to the onset of aortic flow (Q-Ao) by standard pulsed Doppler (SP) or to the onset of mitral annulus systolic wave (Q-Mit) (DTI). The time for right ventricular activation was determined from the onset of QRS to the onset of pulmonary flow (Q-Pulm) (SP) or to the onset of tricuspid annulus systolic wave (Q-Tri) (DTI). (Q-Ao)-(Q-Pulm) and (Q-Mit)-(Q-Tri) determined IMD for each method, respectively. QRS width and IMD showed correlation coefficients of r = 0.86 ([Q-Ao]-[Q-PulmD]) and r = 0.82 ([Q-Mit]-[Q-Tri]) (P less than or equal to 0.001). Mean IMD of 77 +/- 15 ms (SP) and 88 +/- 26 ms (DTI) were noted for QRS width above 150 ms. Left ventricle delayed activation was positively correlated to QRS widening with both methods, (r = 0.90, [Q-Ao]), (r = 0.83, [Q-Mit]) (P less than or equal to 0.001). In conclusion, QRS duration is a good marker of an interventricular mechanical asynchrony. According to IMD correction, left ventricular pacing may be mainly proposed to symptomatic DCM patients with QRS duration > 150 ms. C1 CHU Angers, Dept Cardiol, F-49000 Angers, France. CHU Angers, Dept Stat Biomed, F-49000 Angers, France. RP Rouleau, F, CHU Angers, Dept Cardiol, F-49000 Angers, France. TC 26 PD OCT PY 2001 VL 24 IS 10 BP 1500 EP 1506 UT ISI:000171700700008 ER PT J AU Duverger, C Montagna, M Rolli, R Ronchin, S Zampedri, L Fossi, M Pelli, S Righini, GC Monteil, A Armellini, C Ferrari, M TI Erbium-activated silica xerogels: spectroscopic and optical properties SO JOURNAL OF NON-CRYSTALLINE SOLIDS ID UP-CONVERSION; WAVE-GUIDES; GLASSES; RAMAN; GEL; SPECTRA; IONS; ER3+; LUMINESCENCE; EMISSION AB Silica-based sol-gel glasses activated by Er3+ ions are attractive materials for integrated optics (IO) devices such as frequency upconverters and optical amplifiers. Monolithic erbium-activated silica xerogels with erbium content ranging from 0 up to 40 000 ppm were prepared by the sol-gel technique. Samples were densified by thermal treatment in air at 950 degreesC for 120 h. The densification degree and the relative content of hydroxyl groups were studied by Raman spectroscopy. Refractive indices were measured at 632.8 and 543.5 nm by a prism coupling technique. Green to blue and violet upconversion luminescence upon continuous-wave excitation at 514.5 nm was observed for all samples. Emission at 1.5 mum, characteristic of the I-4(13/2) --> I-4(15/2) transition of Er3+ ions, was observed at room temperature for all samples upon continuous-wave excitation at 980 nm. For the 5000 Er/Si ppm-doped xerogel, a photoluminescence was observed and a lifetime of 8 ms for the metastable I-4(13/2) level was measured. (C) 2001 Elsevier Science B.V. All rights reserved. C1 CNR, CeFSA, Ctr Fis Stati Aggregati, I-38050 Trent, Italy. Univ Trent, Dipartimento Fis, I-38050 Trent, Italy. Univ Angers, CNRS, UMR, Lab POMA, F-49045 Angers, France. CNR, IROE, Optoelect & Photon Dept, I-50127 Florence, Italy. Univ Trent, INFM, I-38050 Trent, Italy. RP Ferrari, M, CNR, CeFSA, Ctr Fis Stati Aggregati, Via Sommarive 14, I-38050 Trent, Italy. TC 26 PD FEB PY 2001 VL 280 IS 1-3 BP 261 EP 268 UT ISI:000167405900039 ER PT J AU Raimundo, JM Blanchard, P Frere, P Mercier, N Ledoux-Rak, I Hierle, R Roncali, J TI Push-pull chromophores based on 2,2 '-bi(3,4-ethylenedioxythiophene) (BEDOT) pi-conjugatiug spacer SO TETRAHEDRON LETTERS DE push-pull chromophores; optical properties; conjugated systems ID NONLINEAR-OPTICAL CHROMOPHORES; HIGHLY EFFICIENT; ACCEPTOR; THIOPHENES; HYPERPOLARIZABILITIES; DERIVATIVES AB Replacement of 2,2'-bithiophene by BEDOT in push-pull NLO-phores produces a red shift of the absorption maximum accompanied with a large increase of the quadratic nonlinear optical susceptibility. (C) 2001 Elsevier Science Ltd. All rights reserved. C1 Univ Angers, CNRS, UMR 6501, F-49045 Angers, France. Ecole Normale Super, Lab Photon Quant & Mol, F-94235 Cachan, France. RP Roncali, J, Univ Angers, CNRS, UMR 6501, 2 Bd Lavoisier, F-49045 Angers, France. TC 26 PD FEB 19 PY 2001 VL 42 IS 8 BP 1507 EP 1510 UT ISI:000167034500028 ER PT J AU Oliver, AE Leprince, O Wolkers, WF Hincha, DK Heyer, AG Crowe, JH TI Non-disaccharide-based mechanisms of protection during drying SO CRYOBIOLOGY DE dehydration, desiccation tolerance; cellular metabolism, free radicals; arbutin; amphiphiles; LEA proteins; fructans ID LOW-MOLECULAR-WEIGHT; TRANSFORM INFRARED MICROSPECTROSCOPY; PLANT MYROTHAMNUS-FLABELLIFOLIA; EMBRYOGENESIS-ABUNDANT PROTEINS; FREEZE-DRIED LIPOSOMES; LIPID BILAYER VESICLES; PISUM-SATIVUM EMBRYOS; LEA MESSENGER-RNAS; ZEA-MAYS L; DESICCATION-TOLERANCE AB Few tissues or organisms can survive the removal of nearly all their intra and extracellular water. These few have developed specialized adaptations to protect their cellular components from the damage caused by desiccation and rehydration. One mechanism, common to almost all such organisms, is the accumulation of disaccharides within cells and tissues at the onset of dehydration. This adaptation has been extensively studied and will not be considered in this review. It has become increasingly clear that true desiccation tolerance is likely to involve several mechanisms working in concert; thus, we will highlight several other important and complimentary adaptations found especially in the dehydration-resistant tissues of higher plants. These include the scavenging of reactive oxygen species, the down-regulation of metabolism, and the accumulation of certain amphiphilic solutes, proteins, and polysaccharides. (C) 2001 Elsevier Science (USA). C1 Univ Calif Davis, Sect Mol & Cellular Biol, Davis, CA 95616 USA. Univ Angers, INH,INRA, UMR Mol Seed Physiol, Inst Natl Hort, F-49045 Angers, France. Max Planck Inst Mol Pflanzenphysiol, D-14424 Potsdam, Germany. RP Oliver, AE, Univ Calif Davis, Sect Mol & Cellular Biol, Davis, CA 95616 USA. TC 25 PD SEP PY 2001 VL 43 IS 2 BP 151 EP 167 UT ISI:000174092700007 ER PT J AU Saulnier, P Boury, F Malzert, A Heurtault, B Ivanova, T Cagna, A Panaiotov, I Proust, JE TI Rheological model for the study of dilational properties of monolayers. Comportment of dipalmitoylphosphatidylcholine (DPPC) at the dichloromethane (DCM)/water interface under ramp type or sinusoidal perturbations SO LANGMUIR ID AIR-WATER-INTERFACE; DIFFERENT FLUID INTERFACES; PHOSPHOLIPID THIN-FILM; SURFACE-TENSION EXCESS; DILATATIONAL PROPERTIES; INSOLUBLE MONOLAYERS; PULMONARY SURFACTANT; AIR/WATER INTERFACE; BETA-LACTOGLOBULIN; DYNAMIC PROPERTIES AB The dilational properties of monolayers are analyzed using the classical linear approximation. In most cases, the observed interfacial behavior can be approached by a model corresponding to a two-dimensional viscoelastic solid. The monolayer is characterized by two dilational elasticity terms (E-e, equilibrium elasticity, and E-ne, nonequilibrium elasticity) and by one relaxation time (tau). These three physical constants are obtained from the responses of a ramp type perturbation, or from the responses (as a function of the frequencies) after sinusoidal area variations. Using axisymmetric drop shape analysis experiments. a dipalmitoyl phosphatidylcholine (DPPC) layer at the dichloromethane/water interface is characterized. Measurements of the surface pressure variations as the response to linear or sinusoidal variations of surface area are performed. Identical rheological physical constants (equilibrium elasticity, nonequilibrium elasticity, and relaxation time) are obtained using both methods. Dilational behavior of DPPC monolayer can be attributed to the molecular diffusion between the DPPC layer and the adjacent phases. C1 INSERM ERIT M 0104, Ingn Vectorisat Particulaire, F-49100 Angers, France. Univ Sofia, Biophys Chem Lab, Sofia 1123, Bulgaria. Interfacial Technol Concept, F-69770 Longessaigne, France. RP Proust, JE, INSERM ERIT M 0104, Ingn Vectorisat Particulaire, Bat IBT,10 Rue A Boquel, F-49100 Angers, France. TC 25 PD DEC 25 PY 2001 VL 17 IS 26 BP 8104 EP 8111 UT ISI:000172956200025 ER PT J AU Leblond, V Levy, V Maloisel, F Cazin, B Fermand, JP Harousseau, JL Remenieras, L Porcher, R Gardembas, M Marit, G Deconinck, E Desablens, B Guilhot, F Philippe, G Stamatoullas, A Guibon, O CA French Cooperative Grp Chronic Lym TI Multicenter, randomized comparative trial of fludarabine and the combination of cyclophosphamide-doxorubicin-prednisone in 92 patients with Waldenstrom macroglobulinemia in first relapse or with primary refractory disease SO BLOOD ID CHRONIC LYMPHOCYTIC-LEUKEMIA; PROGNOSTIC FACTORS; PRIMARY THERAPY; PHASE-II; 2-CHLORODEOXYADENOSINE; RESISTANT; CLADRIBINE; REMISSION; LYMPHOMA; CLASSIFICATION AB Few reports are available on the treatment of patients with Waldenstrom macroglobulinemia (WM) and primary or secondary resistance to alkylating-agent-based regimens. From December 1993 through December 1997, 92 patients with WM resistant to first-line therapy (42) or with first relapse (50) after alkylating-agent therapy were randomly assigned to receive fludarabine (25 mg/m(2) of body-surface area on days 1-5) or cyclophosphamide, doxorubicin (Adriamycin), and prednisone (CAP; 750 mg/m(2) cyclophosphamide and 25 mg/m(2) doxorubicin on day 1 and 40 mg/m(2) prednisone on days 1-5). The first end point evaluated was the response rate after 6 treatment courses. Forty-five patients received CAP and 45 received fludarabine. Two patients died before the first course of chemotherapy. No statistical differences were observed between the 2 treatment arms with respect to hematologic toxicity or Infections. Mucositis and alopecia occurred significantly more often in patients treated with CAP Partial responses were obtained In 14 patients (30%) treated with fludarabine and 5 patients (11%) treated with CAP (P = .019). Responses were more durable in patients treated with fludarabine (19 months versus 3 months), and the event-free survival rate was significantly higher in this group (P < .01). Forty-four patients died, 22 in the fludarabine group and 22 in the CAP group. There was no statistical difference in the median overall survival time in the 2 study arms. Fludarabine was thus more active than CAP in salvage therapy of WM and should be tested as first-line therapy in a randomized comparison with alkylating agents. (C) 2001 by The American Society of Hematology. C1 Hop La Pitie Salpetriere, Dept Hematol, AP, HP, F-75013 Paris, France. Hop St Louis, INSERM, U444, Dept Biostat & Informat Med, Paris, France. Ctr Hosp Reg Univ, Hop Hautepierre, Strasbourg, France. Ctr Hosp Reg Univ, Hop Claude Huriez, Lille, France. Hop St Louis, Serv Immunohematol, AP, HP, Paris, France. CHU Nantes, Hotel Dieu, F-44035 Nantes 01, France. Ctr Hosp Univ, Limoges, France. CHU Angers, Angers, France. Ctr Hosp Univ, Bordeaux, France. CHU Besancon, F-25030 Besancon, France. Ctr Hosp Univ, Amiens, France. Ctr Hosp Univ, Poitiers, France. Ctr Hosp, Pontoise, France. Ctr Anticanc Becquerel, Rouen, France. Lab Schering, Lys Lez Lannoy, France. RP Leblond, V, Hop La Pitie Salpetriere, Dept Hematol, AP, HP, 47 Blvd Hop, F-75013 Paris, France. TC 25 PD NOV 1 PY 2001 VL 98 IS 9 BP 2640 EP 2644 UT ISI:000171855900008 ER PT J AU Guilet, D Helesbeux, JJ Seraphin, D Sevenet, T Richomme, P Bruneton, J TI Novel cytotoxic 4-phenylfuranocoumarins from Calophyllum dispar SO JOURNAL OF NATURAL PRODUCTS ID INHIBITORY NATURAL-PRODUCTS; COUMARINS; PYRANOCOUMARINS; DERIVATIVES; TEYSMANNII; LANIGERUM AB Eight new 4-phenylfuranocoumarins (1-8) have been isolated from the stem bark and the fruits of Calophyllum dispar, together with three known coumarins. The structures of 1-8 were established by means of spectroscopic analysis, including extensive 2D NMR studies. Some of these furanocoumarins exhibited significant cytotoxic activity against KB cells. C1 UFR Sci Pharmaceut & Ingn Sante, SONAS, F-49100 Angers, France. CENS, ICSN, F-91191 Gif Sur Yvette, France. RP Richomme, P, UFR Sci Pharmaceut & Ingn Sante, SONAS, 16 Bd Daviers, F-49100 Angers, France. TC 24 PD MAY PY 2001 VL 64 IS 5 BP 563 EP 568 UT ISI:000168967600003 ER PT J AU Chassevent, A Jourdan, ML Romain, S Descotes, F Colonna, M Martin, PM Bolla, M Spyratos, F CA Multicenter Study Grp PHR C95 TI S-phase fraction and DNA ploidy in 633 T1T2 breast cancers: A standardized flow cytometric study SO CLINICAL CANCER RESEARCH ID THYMIDINE LABELING INDEX; PROGNOSTIC VALUE; QUALITY-CONTROL; PROLIFERATIVE ACTIVITY; ADJUVANT CHEMOTHERAPY; MULTIVARIATE-ANALYSIS; GENETIC EVOLUTION; HISTOLOGIC GRADE; SHORT-TERM; CARCINOMA AB The lack of a standardized methodology for quantifying DNA ploidy and S-phase fraction (SPF) by flow cytometry is hindering routine use of these markers in breast cancer management. In a retrospective clinical multicenter study, we validated a standardized flow cytometry protocol. We tested 633 frozen T1T2, N0N1, M-0 breast tumors obtained in four institutions. Cell preparation was standardized, and precise rules for data interpretation were followed. Three SPF classes were defined on the basis of tertiles after adjustment for ploidy, DNA aneuploidy was observed in 61.0% of cases. No significant difference was observed among centers. Aneuploidy and high SPF were associated with large tumor size, node involvement, high histological grade, and hormone receptor negativity. In the overall population (median followup, 69 months), patients with medium and high SPF values had shorter disease-free survival (DFS) than those with low SPF values (P < 0.0001). Ploidy had no significant influence. By Cox analysis, SPF, pN, and estrogen receptor status were independent predictors of DPS (P = 0.0002, P = 0.001, and P = 0.05). In node-negative patients, SPF was the only predictor of DFS (P = 0.01), whereas in node-positive patients, the risk of relapse increased with both high SPF (P = 0.003) and estrogen receptor negativity (P = 0.004), Low SPF values distinguished grade II tumors with a particularly good outcome. Our results strongly support the use of SPF in multicenter studies and clinical trials and suggest that node-negative patients with slowly proliferating tumors do not require systemic adjuvant therapy. C1 Ctr Paul Papin, CRLCC, F-49036 Angers 01, France. Ctr Rene Huguenin, St Cloud, France. CHU Grenoble, F-38043 Grenoble, France. CHU Lyon Sud, Lyon Sud, France. CHU Tours, Marseille, France. RP Chassevent, A, Ctr Paul Papin, CRLCC, 2 rue Moll, F-49036 Angers 01, France. TC 24 PD APR PY 2001 VL 7 IS 4 BP 909 EP 917 UT ISI:000168297800023 ER PT J AU Allard, E Delaunay, J Cheng, FY Cousseau, J Orduna, J Garin, J TI Novel C-60-based building blocks derived from C-60(2-) anion SO ORGANIC LETTERS ID CHEMICAL GENERATION; ELECTRON-TRANSFER; DERIVATIVES; ELECTROSYNTHESIS; FULLERENES; DYADS AB [GRAPHICS] Reactions of chemically generated C-60(2-) dianion with halo derivatives bearing a second functional group (halo, ester, ketone) give rise to new organo C-60 derivatives. Thus, the regioselective formation of 1,2- and 1,4-adducts is observed from long-chain halo derivatives, whereas using 1,3-dihaloalkanes leads to 1,2-fused ring C-60 adducts. Such adducts may be used as new synthons in C-60 chemistry, thus leading to new C-60-based dyads, as exemplified by two examples. C1 Univ Angers, Lab Ingn Mol & Mat Organ, CNRS, UMR 6501 2 Bd Lavoisier, F-49045 Angers 01, France. Univ Zaragoza, Dept Quim Organ, ICMA, CSIC, E-50009 Zaragoza, Spain. RP Cousseau, J, Univ Angers, Lab Ingn Mol & Mat Organ, CNRS, UMR 6501 2 Bd Lavoisier, F-49045 Angers 01, France. TC 23 PD NOV 1 PY 2001 VL 3 IS 22 BP 3503 EP 3506 UT ISI:000171826200020 ER PT J AU Savagner, F Franc, B Guyetant, S Rodien, P Reynier, P Malthiery, Y TI Defective mitochondrial ATP synthesis in oxyphilic thyroid tumors SO JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM ID UNCOUPLING PROTEIN; ONCOCYTIC TUMORS; DNA; EXPRESSION; NUCLEAR; MYOPATHIES; DISEASES; TISSUES; GLAND; GENE AB Oxyphilic tumors (oncocytomas or Hurthle cell tumors) form a rare subgroup of thyroid tumors characterized by cells containing abundant mitochondria. The relationship between the mitochondrial proliferation and the pathogenesis of these tumors is unknown. We have assessed the expression of the mitochondrial ND2 and ND5 (subunits of the nicotinamide adenine dinucleotide dehydrogenase complex) genes and the nuclear UCP2 (uncoupling protein 2) gene in 22 oxyphilic thyroid tumors and matched controls. The consumption of oxygen in mitochondria from tumors was determined by polarography. ATP assays were used to explore the mitochondrial respiratory chain activity and the oxidative phosphorylation coupling in seven fresh thyroid tumors and controls. Adenosine triphosphate synthesis was significantly lower in all the tumors, compared with controls, suggesting that a coupling defect in oxidative phosphorylation may be a cause of mitochondrial. hyperplasia in oxyphilic thyroid tumors. C1 CHU Angers, Lab Biochim & Biol Mol, INSERM, EMI U 00 18, F-49033 Angers 01, France. Hop Ambroise Pare, Lab Anat Pathol, F-92104 Boulogne, France. Anat Pathol Lab, F-49033 Angers, France. Serv Endocrinol, F-49033 Angers, France. RP Savagner, F, CHU Angers, Lab Biochim & Biol Mol, INSERM, EMI U 00 18, 4 Rue Larrey, F-49033 Angers 01, France. TC 23 PD OCT PY 2001 VL 86 IS 10 BP 4920 EP 4925 UT ISI:000171755400059 ER PT J AU Le Breton, H Boschat, J Commeau, P Brunel, P Gilard, M Breut, C Bar, O Geslin, P Tirouvanziam, A Maillard, L Moquet, B Barragan, P Dupouy, P Grollier, G Berland, J Druelles, P Rihani, R Huret, B Leclercq, C Bedossa, M CA Stent Balloon Predilation Study Gr TI Randomised comparison of coronary stenting with and without balloon predilatation in selected patients SO HEART DE coronary artery angioplasty; stent; coronary artery ultrasound ID INTRACORONARY ULTRASOUND; IMPLANTATION; DEPLOYMENT AB Background-The SWIBAP (stent without balloon predilatation) prospective randomised trial was designed to compare direct coronary stenting with stenting preceded by lesion predilatation with an angioplasty balloon. Objective-To determine the feasibility and safety of direct stenting in non-complex coronary lesions in a prospective study. Patients and design-All patients < 76 years of age scheduled to undergo angioplasty of a noncomplex, non-calcified lesion in a coronary artery of > 3.0 mm, who granted their informed consent, were randomised into the trial. In group I, the stent was placed without balloon predilatation, while in group II stent implantation was preceded by balloon predilatation. The primary end point was the angiographic result according to procedure assigned by randomisation. An intravascular ultrasound substudy was performed in 60 patients. Results-Stent implantation was successful without predilatation in 192 of the 197 group I patients (97.5%), and with predilatation in 197 of the 199 group Il patients (99%) (NS). No in-hospital stent thrombosis or death occurred. Overall procedural times, fluoroscopy times, and volumes of contrast agent given (mean (SD)) in group I upsilon group II were 23.50 (13.54) min upsilon 27.96 (15.23) min (p = 0.002), 6.04 (4.13) min upsilon 6.67 (3.65) min (NS), and 135 (65) ml upsilon 157 (62) ml (p < 0.001), respectively. No major adverse cardiovascular events had occurred by 30 days. Conclusions-The feasibility and safety of direct stenting of selected and non-complex coronary lesions is confirmed. This technique was as successful as the conventional approach and was associated with a minor reduction in fluoroscopic exposure and procedure time and the administration of less contrast agent. C1 CHU Pontchaillou, Ctr Cardiopneumol, Unite Hemodynam & Cardiol Intervent, F-35033 Rennes, France. Ctr Hosp Univ Cavale Blanche, Brest, France. Clin St Martin, Caen, France. Nouvelles Clin Nantaises St Henri, Nantes, France. Clin Grand Large, Brest, France. Clin St Gatien, Tours, France. CHU Angers, Angers, France. CHU Nantes, F-44035 Nantes 01, France. CHU Tours, Tours, France. Clin Reine Blanche, Orleans, France. Ctr Hosp Beauregard, Marseille, France. Ctr Hosp Univ Henri Mondor AP HP, F-94010 Creteil, France. CHU Caen, F-14000 Caen, France. Clin St Hilaire, Rouen, France. Clin St Laurent, Rennes, France. Ctr Hosp St Philibert, Lomme Les Lille, France. RP Le Breton, H, CHU Pontchaillou, Ctr Cardiopneumol, Unite Hemodynam & Cardiol Intervent, Rue Henri Le Guilloux, F-35033 Rennes, France. TC 23 PD SEP PY 2001 VL 86 IS 3 BP 302 EP 308 UT ISI:000170741800018 ER PT J AU Khodorkovsky, V Shapiro, L Krief, P Shames, A Mabon, G Gorgues, A Giffard, M TI Do pi-dimers of tetrathiafulvalene cation radicals really exist at room temperature? SO CHEMICAL COMMUNICATIONS ID DENSITY-FUNCTIONAL THEORY; BEDT-TTF; ELECTROCHEMICAL PROPERTIES; CHEMISTRY; SPECTRA; SALT AB The longest wave absorption band of the tetramethylthiotetrathiafulvalene cation radical, which is usually interpreted as a pi -dimer band, is shown to be the intrinsic cation radical absorption, all studied cation radicals in solution at room temperature exist as paramagnetic monomers and only tetrathiafulvelene and tetramethyltetrathiafulvalene cation radicals undergo pi -dimerization at low temperatures. C1 Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel. Ben Gurion Univ Negev, Dept Phys, IL-84105 Beer Sheva, Israel. Univ Angers, CNRS, UMR 6501, F-49045 Angers, France. RP Khodorkovsky, V, Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel. TC 22 PD DEC 21 PY 2001 IS 24 BP 2736 EP 2737 UT ISI:000173044900105 ER PT J AU Rouleau, F Asfar, P Boulet, S Dube, L Dupuis, JM Alquier, P Victor, J TI Transient ST segment elevation in right precordial leads induced by psychotropic drugs: Relationship to the Brugada syndrome SO JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY DE fluoxetine; amitriptyline; phenothiazine; ST segment elevation; sodium channel; proarrhythmia ID BUNDLE-BRANCH BLOCK; ANTIDEPRESSANT DRUGS; SODIUM-CHANNELS; MECHANISM; OVERDOSE; FIBERS; DEATH AB Psychotropic Drugs and ST Segment Elevation. Transient ST segment elevation in right precordial leads with use of psychotropic drugs is reported in two cases of overdose and one case of therapeutic administration. Flecainide did not reproduce ST segment elevation. The relationship of these abnormalities to the Brugada syndrome and the electrophysiologic hypothesis are discussed. C1 CHU Angers, Dept Cardiol, F-49000 Angers, France. CHU Angers, Dept Reanimat Med, F-49000 Angers, France. RP Rouleau, F, CHU Angers, Dept Cardiol, Rue Larrey, F-49000 Angers, France. TC 22 PD JAN PY 2001 VL 12 IS 1 BP 61 EP 65 UT ISI:000166538500012 ER PT J AU Coutant, R de Casson, FB Douay, O Mathieu, E Rouleau, S Beringue, F Gillard, P Limal, JM Descamps, P TI Relationships between placental GH concentration and maternal smoking, newborn gender, and maternal leptin: Possible implications for birth weight SO JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM ID GROWTH-HORMONE SECRETION; IGF-BINDING PROTEIN-3; BODY-MASS INDEX; INSULIN-LIKE; FACTOR-I; CORD-BLOOD; PHYSIOLOGICAL-ROLE; CIGARETTE-SMOKING; PREGNANT-WOMEN; HUMAN FETUSES AB The control of fetal growth depends on multiple hormones, including both IGF-I and placental GH (PGH) in the mother, and IGF-I rather than pituitary GH (pitGH) in the fetus. Leptin, which is produced by adipocytes and syncitiotrophoblast cells, has also been thought to influence fetal growth by an as yet unknown mechanism. This study assessed the relationships between the GH-IGF-I axis in mothers and newborns, and maternal smoking, neonate gender, and maternal and fetal leptin. We collected blood in 87 mothers at the onset of labor and cord blood immediately after birth in their 87 healthy full-term newborns. GH concentrations were log(10) transformed, and data were expressed as the geometric mean (-1, +1 tolerance factor). PGH was lower in the 30 smoking mothers, as compared with the 57 nonsmoking mothers [18.2 (11.5; 28.6) vs. 27.0 (15.1; 48.2) mug/liter, P < 0.01]. Cord blood IGF-I was lower in neonates from smoking mothers (90 +/- 44 vs. 135 +/- 65 g/liter, mean +/- SD, P < 0.01), consistent with their lower birth weight percentile (P < 0.01). A gender effect was observed for PGH, which was higher when the newborn was female, and for newborn pitGH and newborn leptin, which were, respectively, lower and higher in females, even after adjustment for birth weight and maternal smoking category (P < 0.05 for all comparisons). Multiple regression analyses identified maternal leptin as a negative predictor of PGH (P < 0.05) and newborn leptin as a positive predictor of newborn IGF-I (P < 0.05). Maternal smoking is associated to decreased maternal PGH and cord blood IGF-I concentrations. A sexual dimorphism for PGH, newborn pitGH, and newborn leptin exists at the time of birth, but its physiological significance remains to be studied. The relationships between maternal leptin and PGH and between cord blood leptin and IGF-I are consistent with the hypothesis that leptin could contribute to the control of fetal growth. C1 Univ Hosp, Dept Pediat, F-49000 Angers, France. Univ Hosp, Dept Nucl Med, F-49000 Angers, France. Univ Hosp, Dept Biochem & Mol Biol, F-49000 Angers, France. Univ Hosp, Dept Obstet & Gynecol, F-49000 Angers, France. RP Coutant, R, Univ Hosp, Dept Pediat, 4 Rue Larrey, F-49000 Angers, France. TC 21 PD OCT PY 2001 VL 86 IS 10 BP 4854 EP 4859 UT ISI:000171755400049 ER PT J AU Chen, JM Cutler, C Jacques, C Boeuf, G Denamur, E Lecointre, G Mercier, B Cramb, G Ferec, C TI A combined analysis of the cystic fibrosis transmembrane conductance regulator: Implications for structure and disease models SO MOLECULAR BIOLOGY AND EVOLUTION DE cystic fibrosis transmembrane conductance regulator; missense mutation; structure and disease models; phylogeny; Atlantic salmon; rabbit ID NUCLEOTIDE-BINDING DOMAINS; CFTR CHLORIDE CHANNEL; ANION-SELECTIVITY; R-DOMAIN; DEVELOPMENTAL EXPRESSION; SEQUENCE-ANALYSIS; ABC TRANSPORTER; GENE; MUTATIONS; IDENTIFICATION AB Over the past decade, nearly 1,000 variants have been identified in the cystic fibrosis transmembrane conductance regulator (CFTR) gene in classic and atypical cystic fibrosis (CF) patients worldwide, and an enormous wealth of information concerning the structure and function of the protein has also been accumulated. These data, if evaluated together in a sequence comparison of all currently available CFTR homologs, are likely to refine the global structure-function relationship of the protein, which will, in turn, facilitate interpretation of the identified mutations in the gene. Based on such a combined analysis, we had recently defined a "functional R domain" of the CFTR protein. First, presenting two full-length cDNA sequences (termed sCFTR-I and sCFTR-II) from the Atlantic salmon (Salmo salar) and an additional partial coding sequence from the eastern gray kangaroo (Macropus giganteus), this study went further to refine the boundaries of the two nucleotide-binding domains (NBDs) and the COOH-terminal tail (C-tail), wherein NBDI was defined as going from P439 to G646, NBD2 as going from A1225 to E1417, and the C-tail as going from E1418 to L1480. This approach also provided further insights into the differential roles of the two halves of CFTR and highlighted several well-conserved motifs that may be involved in inter- or intramolecular interactions. Moreover, a serious concern that a certain fraction of missense mutations identified in the CFTR gene may not have functional consequences was raised. Finally, phylogenetic analysis of all the full-length CFTR amino acid sequences and an extended set of exon 13-coding nucleotide sequences reinforced the idea that the rabbit may represent a better CF model than the mouse and strengthened the assertion that a long-branch attraction artifact separates the murine rodents from the rabbit and the guinea pig, the other Glires. C1 Univ Bretagne Occidentale, Ctr Hosp Univ, Etab Francais Sang Bretagne, INSERM EMI 01 15, F-29275 Brest, France. Univ St Andrews, Sch Biol, St Andrews, Fife, Scotland. CHU Angers, Lab Biochim Biol Mol, Angers, France. Univ Paris 06, CNRS, Observ Oceanol, Banyuls sur Mer, France. Hop Robert Debre, INSERM, U458, Paris, France. Museum Natl Hist Nat, Serv Syst Mol, Paris, France. RP Ferec, C, Univ Bretagne Occidentale, Ctr Hosp Univ, Etab Francais Sang Bretagne, INSERM EMI 01 15, 46 Rue Felix Le Dantec, F-29275 Brest, France. TC 21 PD SEP PY 2001 VL 18 IS 9 BP 1771 EP 1788 UT ISI:000170629700015 ER PT J AU Coutant, R Lumbroso, S Rey, R Lahlou, N Venara, M Rouleau, S Sultan, C Limal, JM TI Macroorchidism due to autonomous hyperfunction of Sertoli cells and G(s)alpha gene mutation: An unusual expression of McCune-Albright syndrome in a prepubertal boy SO JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM ID ANTI-MULLERIAN HORMONE; POLYOSTOTIC FIBROUS DYSPLASIA; STIMULATORY G-PROTEIN; CLINICAL IMPLICATIONS; ACTIVATING MUTATIONS; SEXUAL PRECOCITY; MOLECULAR-BASIS; G-ALPHA(S); DEFECTS; MICE AB We report an unusual observation of a 3.8-yr-old boy with McCune-Albright syndrome (MAS) associated with abnormal prepubertal testis enlargement and no sexual precocity. Physical examination showed cafe-au-lait skin lesions, enlarged testes. prepubertal sized penis, and no pubic or axillary hair. Skeletal radiography disclosed fibrous dysplasia. The serum testosterone level was 0.58 nmol/L and remained below 1.4 nmol/L during the 4-yr follow-up. By contrast, serum inhibin B and anti-Mullerian hormone concentrations were abnormally increased up to 255 pg/mL (childhood range, 35-180) and 792 pmol/L (childhood range, 309-566), respectively. The LH response to a GnRH test was in the prepubertal range, whereas the FSH response was blunted. This abnormal hormone concentration profile indicates autonomous hyperfunction of Sertoli cells, with no evidence of Leydig cell activation. Testicular histology showed tubules with marked Sertoli cell hyperplasia and very rare germinal cells, and interstitial tissue containing mesenchymal cells but no mature Leydig cells. DNA sequence analysis from bone and testis tissues detected the known activating mutation in MAS that results in replacement of Arg by His at codon 201 of the G(s)alpha protein. Other endocrine tests showed excessive GH secretion and moderate adrenal androgen hypersecretion. These findings are consistent with the occurrence of an activating mutation of the G(s)alpha gene mainly expressed in Sertoli cells and weakly expressed or absent in Leydig cells. Abnormal prepubertal testicular enlargement extends the clinical spectrum of MAS, suggesting that determination of serum inhibin B and anti-Mullerian hormone should be considered in boys with this syndrome. This observation demonstrates the usefulness of detailed molecular and biological investigations in atypical cases of MAS. C1 Hop St Vincent de Paul, Hormonal Lab, F-75674 Paris, France. Hosp Ninos Dr Ricardo Gutierrez, Ctr Invest Endocrinol, RA-1425 Buenos Aires, DF, Argentina. Ecole Normale Super, INSERM U493, Unite Rech Endocrinol Dev, F-92120 Montrouge, France. CHU Montpellier, INSERM, U439, Lab Hormonol, F-34090 Montpellier, France. CHU Angers, Dept Pediat, F-49000 Angers, France. RP Coutant, R, Univ Hosp, Dept Pediat, 4 Rue Larrey, F-49000 Angers, France. TC 21 PD APR PY 2001 VL 86 IS 4 BP 1778 EP 1781 UT ISI:000168243000056 ER PT J AU Jouad, EM Riou, A Allain, M Khan, MA Bouet, GM TI Synthesis, structural and spectral studies of 5-methyl 2-furaldehyde thiosemicarbazone and its Co, Ni, Cu and Cd complexes SO POLYHEDRON DE furan thiosemicarbazone; 3d metals complexes; crystallographic structures ID COPPER(II) COMPLEXES; CRYSTAL-STRUCTURE; NICKEL(II) COMPLEXES; BIOLOGICAL-ACTIVITY AB The reaction of cobalt, nickel, copper and cadmium chlorides and bromides with 5-methylfurfural thiosemicarbazone (M5FTSC) leads to the formation of two series of new complexes: [M(M5FTSC)(2)X-2], [M(M5FTSC)X-2]. They have been characterized by spectroscopic studies (infrared, H-1 NMR, and electronic spectral. The crystal structures of the free ligand M5FTSC and of the compound [CuCl2(M5FTSC)] have been determined by X-ray diffraction methods. For the Co(II), Ni(II) and Cu(II) complexes, the central atom is coordinated through the sulphur atom and the azomethine nitrogen atom whilst for the Cd(II) complexes, the coordination atoms are the sulphur and furanic oxygen atoms instead of the azomethine nitrogen. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Fac Pharm Angers, F-49100 Angers, France. Fac Sci, CNRS, IMMO, UMR 6501, F-49045 Angers, France. RP Bouet, GM, Fac Pharm Angers, 16 Blvd Daviers, F-49100 Angers, France. TC 21 PD JAN 15 PY 2001 VL 20 IS 1-2 BP 67 EP 74 UT ISI:000167008300006 ER PT J AU Bailly, C Audigier, C Ladonne, F Wagner, MH Coste, F Corbineau, F Come, D TI Changes in oligosaccharide content and antioxidant enzyme activities in developing bean seeds as related to acquisition of drying tolerance and seed quality SO JOURNAL OF EXPERIMENTAL BOTANY DE antioxidant enzymes; bean seed; desiccation tolerance; oligosaccharides; Phaseolus vulgaris L.; seed development ID DESICCATION-TOLERANCE; PHASEOLUS-VULGARIS; HYDROGEN-PEROXIDE; SOLUBLE SUGARS; GLUTATHIONE-REDUCTASE; SUNFLOWER SEEDS; MATURATION; GERMINATION; STORAGE; PLANTS AB Seeds of bean (Phaseolos vulgaris cv. Vernel) were collected throughout their development on the plant and dried at 15 degreesC and 75% relative humidity to a final moisture content of about 16% (fresh weight basis) to determine whether the onset of tolerance to this drying condition was related to changes in soluble sugars or the activities of the main antioxidant enzymes, namely superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR). Measurements of soluble sugars and enzyme activities were made after drying the seeds, and drying tolerance was evaluated by the ability of dried seeds to germinate and to produce normal seedlings. Seeds became tolerant to drying at 45 d after anthesis, a time marking physiological maturity. At physiological maturity, the moisture content of seeds was about 50-55% (fresh weight basis) and seed dry matter reached about 190 mg per seed. Seed vigour, evaluated by controlled deterioration and conductivity measurements, continued to increase after seed mass maturity, but decreased when seeds remained thereafter for more than 7 d on the plant. Acquisition of drying tolerance was coincident with an accumulation of raffinose and stachyose. Dried-tolerant seeds were also characterized by a high amount of sucrose, the most abundant sugar, and by a low content of monosaccharides. The (raffinose + stachyose)/sucrose ratio increased during seed filling, reaching a value close to 1 when all the seeds became tolerant to drying, and maintaining this proportion during the final stages of maturation. Acquisition of drying tolerance was also related to a reorientation of the enzymatic antioxidant defence system. Drying-tolerant dried seeds displayed high CAT and GR activities and low SOD and APX activities, while the opposite condition was observed in immature dried seeds. The shift in antioxidant enzymes corresponded to the beginning of the maturation-drying phase. These results suggest that oligosaccharide metabolism and enzymatic antioxidant defences may be involved in acquisition of drying tolerance during bean seed development, but are not related to seed vigour. C1 Univ Paris 06, F-75252 Paris 05, France. BIOCEM, F-49070 Beaucouze, France. LABOSEM, FNAMS, F-49800 Brain Sur Authion, France. SNES, GEVES, F-49071 Beaucouze, France. ESA, Lab Ecophysiol & Agron, F-49007 Angers 01, France. RP Bailly, C, Univ Paris 06, Tour 53,1er Etage,4 Pl Jussieu, F-75252 Paris 05, France. TC 20 PD APR PY 2001 VL 52 IS 357 BP 701 EP 708 UT ISI:000169703200006 ER PT J AU Liu, SG Martineau, C Raimundo, JM Roncali, J Echegoyen, L TI Formation and electrochemical desorption of stable and electroactive self-assembled monolayers (SAMs) of oligothiophene-fulleropyrrolidine dyads SO CHEMICAL COMMUNICATIONS ID RECOGNITION; JUNCTION AB Stable, electroactive SAMs of oligothiophene-fulleropyrrolidine dyads have been prepared by spontaneous adsorption; electro-oxidation of the oligomeric system results in desorption. C1 Univ Angers, CNRS, UMR 6501, F-49045 Angers, France. Univ Miami, Dept Chem, Miami, FL 33143 USA. Natl Renewable Energy Lab, Golden, CO 80401 USA. RP Roncali, J, Univ Angers, CNRS, UMR 6501, 2 Bd Lavoisier, F-49045 Angers, France. TC 20 PY 2001 IS 10 BP 913 EP 914 UT ISI:000168558200018 ER PT J AU Cezard, JP Duhamel, JF Meyer, M Pharaon, I Bellaiche, M Maurage, C Ginies, JL Vaillant, JM Girardet, JP Lamireau, T Poujol, A Morali, A Sarles, J Olives, JP Whately-Smith, C Audrain, S Lecomte, JM TI Efficacy and tolerability of racecadotril in acute diarrhea in children SO GASTROENTEROLOGY ID ENKEPHALINASE INHIBITOR; ORAL REHYDRATION; ACETORPHAN; LOPERAMIDE AB Background&Aims: Oral rehydration therapy is the only treatment recommended by the World Health Organization in acute diarrhea in children. Antisecretory drugs available could not be used because of their side effects, except for racecadotril, which is efficient in acute diarrhea in adults. Methods: The efficacy and tolerability of racecadotril (1.5 mg/kg administered orally 3 times daily) as adjuvant therapy to oral rehydration were compared with those of placebo in 172 infants aged 3 months to 4 years (mean age, 12.8 months) who had acute diarrhea, The treatment groups were comparable in terms of age, duration of diarrhea, number of stools, and causative microorganism at Inclusion. Results: During the first 48 hours of treatment, patients receiving racecadotril had a significantly lower stool output (grams per hour) than those receiving placebo. The 95% confidence interval was 43%-88% for the full data set (n = 166; P = 0.009) and 33%-75% for the per-protocol population (n = 116; P = 0.001), There was no difference between treatments depending on rotavirus status. Significant differences between treatment groups were also found after 24 hours of treatment: full data set (n = 167; P = 0.026) and per-protocol population (n = 121; P = 0.015), Tolerability was good in both groups of patients. Conclusions: This study demonstrates the efficacy (up to 50% reduction in stool output) and tolerability of racecadotril as adjuvant therapy to oral rehydration solution in the treatment of severe diarrhea in infants and children. C1 Pediat Gastroenterol Unit, Paris, France. Pediat Gastroenterol Unit, Caen, France. Pediat Gastroenterol Unit, Clermont Ferrand, France. Pediat Gastroenterol Unit, Versailles, France. Pediat Gastroenterol Unit, Tours, France. Pediat Gastroenterol Unit, Angers, France. Pediat Gastroenterol Unit, Lisieux, France. Pediat Gastroenterol Unit, Bordeaux, France. Pediat Gastroenterol Unit, Aix En Provence, France. Pediat Gastroenterol Unit, Nancy, France. Pediat Gastroenterol Unit, Marseille, France. Pediat Gastroenterol Unit, Toulouse, France. SmithKline Beecham Int, London, England. Lab Bioprojet, Paris, France. RP Cezard, JP, Hop Robert Debre, Serv Gastroenterol & Nutr Pediat, 48 Blvd Serurier, F-75019 Paris, France. TC 20 PD MAR PY 2001 VL 120 IS 4 BP 799 EP 805 UT ISI:000167302300006 ER PT J AU Cabrol, D Gillet, JY Madelenat, P Lansac, J Paniel, B Dellenbach, P Lemoine, JP Bronstein, R Leng, JJ Laurent, MC Mares, P Hedon, B Magnin, G Treisser, A Schumacher, JC Grosieux, P Jacquetin, P Bruhat, M Segard, L Puech, F Engelmann, P Schweitzer, M Boog, G Boubli, L Duquidt, N Desroches, A Blot, P Milliez, J Tabaste, JL MacLennan, AH Crowther, C Dickinson, JE Peat, BB King, J Fulcher, IR Permezel, JM CA French Australian Atosiban Investi TI Treatment of preterm labor with the oxytocin antagonist atosiban: a double-blind, randomized, controlled comparison with salbutamol SO EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY DE atosiban; preterm labor; salbutamol; beta-adrenergic agonist; oxytocin antagonists ID CONTROLLED TRIALS; HUMAN PARTURITION; PULMONARY-EDEMA; PREMATURE LABOR; PREGNANCY; RECEPTORS; RITODRINE; DELIVERY; MORTALITY; MORBIDITY AB Objective: To compare the efficacy and safety of atosiban and salbutamol in the treatment of preterm labor. Studs, design: A multicenter, double-blind, double-placebo, randomized, controlled trial. Women (n = 241) diagnosed with preterm labor at 23-33 gestational weeks were enrolled and received either atosiban (n = 119) or salbutamol (n = 122). At randomization, women were stratified by gestational age (less than or equal to 28 weeks and > 28 weeks). Atosiban (i.v. bolus dose of 6.75 mg, then 300 mug/min for 3 h and 100 mug/rain for up to 48 h) and salbutamol (2.5-45 mug/min) were administered by i.v. infusion for up to 48 h. Retreatment with study drug or an alternative tocolytic agent was allowed. Main outcome measures included tocolytic effectiveness which was assessed in terms of the number of women undelivered after 48 h and 7 days. Tocolytic efficacy and tolerability were assessed in terms of the proportion of women undelivered and who did not require alternative tocolytic therapy at 48 h and 7 days of starting treatment. Safety was assessed in terms of maternal side effects and neonatal morbidity. Results: Tocolytic effectiveness at 48 It was 93.3 versus 95.0% (P = 0.67) and after 7 days was 89.9 versus 90.1% (P = 0.93) in the atosiban and salbutamol groups, respectively, Tocolytic efficacy and tolerability within 48 h was 79.8 versus 75.2% (P = 0.15), and after 7 days was 58.8 versus 46.3% (P = 0.021) in the atosiban and salbutamol groups, respectively. Maternal adverse events, including serious events, occurred more frequently in the salbutamol group. Neonatal outcomes were comparable between the study groups. Conclusions: The oxytocin antagonist atosiban was found to be better tolerated by both mother and fetus than salbutamol, with a comparable neonatal and infant safety profile, and atosiban was as effective as salbutamol in delaying threatened preterm birth. This study supports the clinical use of atosiban in the treatment of preterm labor. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved. C1 Grp Hosp Cochin, Serv Gynecol Obstet 1, Matern Port Royal, F-75014 Paris, France. Archet II Hosp, Nice, France. Hop Xavier Bichat, Paris, France. Ctr Hosp Intercommunal, Creteil, France. Ctr Medicochirurg Schilitigheim, Strasbourg, France. Hop Charles Nicolle, F-76031 Rouen, France. CHR Pellegrin Tripode Hosp, Bordeaux, France. CHR Sud Hosp, Rennes, France. CHRU, Caremeau Hosp, Nimes, France. CHRU, Matern Arnaud De Villeneuve, Montpellier, France. Cite Hosp Miletrie, Poitiers, France. Civil Hosp, Strasbourg, France. Hasenrein Hosp, Mulhouse, France. Hop Hotel Dieu, Angers, France. Hotel Dieu Matern Hosp, Clermont Ferrand, France. Hotel Dieu Polyclin Hosp, Clermont Ferrand, France. Jean Rostand Hosp, Sevres, France. Jeanne De Flandre Hosp, Lille, France. Hop Louis Mourier, F-92701 Colombes, France. Matern Reg Adolphe Pinard, Nancy, France. Mere Enfant Hosp, Nantes, France. Nord Hosp, Marseille, France. Hop Notre Dame de Bon Secours, Metz, France. Orleans Hosp, Orleans, France. Hop Robert Debre, F-75019 Paris, France. Hop St Antoine, F-75571 Paris, France. Univ Limoges, Hosp Dupuytren, Limoges, France. Womens & Childrens Hosp, Adelaide, SA, Australia. King Edward Mem Hosp Women, Subiaco, WA 6008, Australia. King George V Mem Hosp, Camperdown, NSW 2050, Australia. Mater Mothers Hosp, Brisbane, Qld, Australia. Nepean Hosp, Kingswood, NSW, Australia. Royal Womens Hosp, Carlton, Vic, Australia. Bocage Hosp, Tours, France. RP Cabrol, D, Grp Hosp Cochin, Serv Gynecol Obstet 1, Matern Port Royal, 123 Blvd Port Royal, F-75014 Paris, France. TC 19 PD OCT PY 2001 VL 98 IS 2 BP 177 EP 185 UT ISI:000171779000005 ER PT J AU Legrand, E Hedde, C Gallois, Y Degasne, I De Casson, FB Mathieu, E Basle, MF Chappard, D Audran, M TI Osteoporosis in men: A potential role for the sex hormone binding globulin SO BONE DE male osteoporosis; sex hormone binding globulin; estradiol; bone mineral density; bone remodeling markers ID BONE-MINERAL DENSITY; VERTEBRAL FRACTURES; AROMATASE DEFICIENCY; WOMEN; PREVALENCE; ESTRADIOL; ESTROGENS; MUTATION; MASS; RISK AB The exact mechanism of bone loss remains unknown in primary male osteoporosis. It has been suggested that estrogen and sex hormone binding globulin (SHBG) play a role in regulating bone turnover and bone mass in healthy men > 65 years of age. In the present study, 80 men (mean age 49.7 years) with bone mineral density >2.5 SD below the young adult value and 40 age-matched controls were recruited to evaluate the relationships between sex hormone levels, bone biochemical markers levels, and bone mineral density. Fasting serum samples were assayed for total and free testosterone total estradiol, and SHBG. The free androgen index, was calculated as: [total testosterone/SHBG * 100]. Bone remodeling was evaluated by measurement of urinary levels of the C-telopeptide of type I collagen (CTx) and free deoxypyridinoline (D-Pyr), serum osteocalcin, and bone-specific alkaline phosphatase (bSAP). There was no significant difference between controls and osteoporotic men according to age, body mass index (BMI), total testosterone, and estradiol. In contrast, serum SHBG level was significantly higher (+42.2%), whereas free androgen index was lower (-24.8%) in patients with primary or secondary osteoporosis. Testosterone and estradiol levels did not correlate with any bone resorption or bone formation markers. In contrast, stepwise linear regression analysis showed that SHBG was significantly correlated with D-Pyr (r = 0.45, p < 0.05) and CTx (r = 0.34, p < 0.05) in primary osteoporosis. In secondary osteoporosis, SHBG was correlated with D-Pyr (r = 0.48,p < 0.05) and bSAP (r = 0.55,p < 0.01). After adjustment for age and BMI, hip bone mineral density (BMD) was not associated with testosterone or estradiol but only with serum SHBG (r = -0.33, p < 0.01) in primary osteoporosis. The same relationship was observed in men with secondary osteoporosis (r = -0.34,p < 0.01). Among osteoporotic patients, spinal radiography showed at least one vertebral crush fracture in 36 men and none in 44. Serum SHBG concentration was significantly associated with the presence of vertebral fracture: the odds ratio was 2.0 (95% confidence interval [CI] 1.2-3.5) for an increase of one standard deviation of SHBG. In conclusion, the present study showed that serum SHBG concentration is increased in middle-aged men with primary or secondary osteoporosis and is correlated with bone remodeling markers, hip bone mineral density, and vertebral fracture risk. (C) 2001 by Elsevier Science Inc. All rights reserved. C1 CHU Angers, Serv Rhumatol, F-49033 Angers, France. CHU Angers, Lab Biochim & Biol Mol, F-49033 Angers, France. CHU Angers, Lab Med Nucl & Biophys, F-49033 Angers, France. CHU Angers, Lab Histol Embryol, F-49033 Angers, France. RP Legrand, E, CHU Angers, Serv Rhumatol, F-49033 Angers, France. TC 19 PD JUL PY 2001 VL 29 IS 1 BP 90 EP 95 UT ISI:000170219400014 ER PT J AU Bagwell, CB Clark, GM Spyratos, F Chassevent, A Bendahl, PO Stal, O Killander, D Jourdan, ML Romain, S Hunsberger, B Baldetorp, B TI Optimizing flow cytometric DNA ploidy and S-phase fraction as independent prognostic markers for node-negative breast cancer specimens SO CYTOMETRY DE breast cancer; flow cytometry; DNA index; DNA ploidy; S-phase; prognosis ID PARAFFIN-EMBEDDED TISSUE; FALSE ANEUPLOID PEAKS; CELL-CYCLE ANALYSIS; HUMAN SOLID TUMORS; PROLIFERATIVE ACTIVITY; KINETIC INFORMATION; FOLLOW-UP; STAGE-I; INDEX; DISTRIBUTIONS AB Developing a reliable and quantitative assessment of the potential virulence of a malignancy has been a long-standing goal in clinical cytometry, DNA histogram analysis provides valuable information on the cycling activity of a tumor population through S-phase estimates; it also identifies nondiploid populations, a possible indicator of genetic instability and subsequent predisposition to metastasis. Because of conflicting studies in the literature, the clinical relevance of both of these potential prognostic markers has been questioned for the management of breast cancer patients. The purposes of this study are to present a set of 10 adjustments derived from a single large study that optimizes the prognostic strength of both DNP, ploidy and S-phase and to test the validity of this approach on two other large multicenter studies. Ten adjustments to both DNA ploidy and S-phase were developed from a single node-negative breast cancer database from Baylor College (n = 961 cases), Seven of the adjustments were used to reclassify histograms into low-risk and high-risk ploidy patterns based on aneuploid fraction and DNA index optimum thresholds resulting in prognostic P values changing from little (P < 0.02) or no significance to P < 0.000005, Other databases from Sweden (n = 210 cases) and France (n = 220 cases) demonstrated similar improvement of DNA ploidy prognostic significance, P < 0.02 to P < 0.0009 and P < 0.12 to P < 0,002, respectively. Three other adjustments were applied to diploid and aneuploid S-phases, These adjustments eliminated a spurious correlation between DNA ploidy and S-phase and enabled them to combine independently into a powerful prognostic model capable of stratifying patients into low, intermediate, and high-risk groups (P < 0.000005), When the Baylor prognostic model was applied to the Sweden and French databases, similar significant patient stratifications were observed (P < 0.0003 and P < 0.00001, respectively). The successful transference of the Baylor prognostic model to other studies suggests that the proposed adjustments may play an important role in standardizing this test and provide valuable prognostic information to those involved in the management of breast cancer patients. (C) 2001 Wiley-Liss, Inc. C1 Ver Software House Inc, Topsham, ME 04086 USA. Baylor Coll Med, Breast Ctr, Houston, TX USA. Ctr Rene Huguenin, St Cloud, France. Ctr Paul Papin, Angers, France. Univ Lund, Univ Hosp, Dept Oncol, Jubileum Inst, Lund, Sweden. Linkoping Univ, Fac Hlth Sci, Dept Oncol, Linkoping, Sweden. Hop Bretonneau, Tours, France. Assistance Publ Hop Marseille, Marseille, France. RP Bagwell, CB, Ver Software House Inc, POB 247, Topsham, ME 04086 USA. TC 19 PD JUN 15 PY 2001 VL 46 IS 3 BP 121 EP 135 UT ISI:000169813400001 ER PT J AU Allamargot, C Pouplard-Barthelaix, A Fressinaud, C TI A single intracerebral microinjection of platelet-derived growth factor (PDGF) accelerates the rate of remyelination in vivo SO BRAIN RESEARCH DE demyelination; oligodendrocyte progenitor; oligodendrocyte; 2 ',3 '-cyclic nucleotide 3 '-phosphodiesterase myelin basic protein ID CENTRAL-NERVOUS-SYSTEM; INDUCED FOCAL DEMYELINATION; O-2AADULT PROGENITOR CELLS; RABBIT CORPUS-CALLOSUM; MYELIN-LIKE MEMBRANES; SPINAL-CORD; IN-VITRO; OLIGODENDROCYTE DEVELOPMENT; LYSOPHOSPHATIDYL CHOLINE; MATURE OLIGODENDROCYTES AB We had demonstrated that platelet-derived growth factor (PDGF) enhanced the reconstruction of myelin-like membranes after their disruption by lysophosphatidylcholine (LPC) in vitro. To investigate its role in vivo. a demyelinating lesion of the corpus callosum was induced in adult Wistar rats by a stereotaxic microinjection of 1 mul LPC, then 63 pairs of rats received either 1 mug PDGF, or its vehicle buffer which were injected above LPC. The effects of PDGF were significant after 2 weeks: the number of oligodendrocytes (OL) expressing 2',3'-cyclic nucleotide 3'-phosphodiesterase in the lesion increased by 49%, mature OL labelled by in situ hybridization for myelin basic protein-mRNA increased by 27% (P < 10(-2)), and the total volume of demyelination decreased by 60% compared to controls. The proliferation of cells of the OL lineage was also enhanced up to 67% by PDGF compared to LPC controls (P <2.5x10(-2)). Ultrastructural studies confirmed this dramatic improvement, and the ratio of remyelinated to demyelinated axons, determined at the maximal demyelination site, in the centre of the lesion, increased by 10-fold (P <2.5x10(-3)) in animals treated with PDGF. Remyelination was complete after 3 months for both treatments. Neither exacerbation of gliosis nor glial tumoural transformation were observed. Mechanisms through which PDGF improves remyelination could involve proliferation of OL progenitors, and/or of already differentiated surviving OLs, and a chemotactic effect, which had been identified in vitro. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Hosp Angers, Dept Neurol, F-49033 Angers 01, France. Univ Hosp Angers, Dept Cell Biol, F-49033 Angers, France. RP Fressinaud, C, Univ Hosp Angers, Dept Neurol, 4 Rue Larrey, F-49033 Angers 01, France. TC 18 PD NOV 9 PY 2001 VL 918 IS 1-2 BP 28 EP 39 UT ISI:000172302100004 ER PT J AU Kreher, D Liu, SG Cariou, M Hudhomme, P Gorgues, A Mas, M Veciana, J Rovira, C TI Novel [60]fullerene-TTF cyclohexene fused polyadducts: unprecedented tri- and tetra-Diels-Alder adducts of dimethylidene[2H]tetrathiafulvalenes with C-60 SO TETRAHEDRON LETTERS ID ELECTRON-TRANSFER; TETRATHIAFULVALENE TTF; BIS-LINKING; PI-DONORS; DYADS; DERIVATIVES; RADICALS AB The title compounds, which are formed in very low yields by treating 2,3-bis(bromomethyl)tetrathiafulvalenes with naked iodide in the presence of C-60, can be obtained in much higher yields by successive similar treatments of the major adducts produced at each step. The electrochemical properties of the unprecedented tri- and tetra-TTF/C-60 assemblies are also presented. (C) 2001 Elsevier Science Ltd. All rights reserved. C1 Univ Angers, Lab Ingn Mol & Mat Organ, CNRS, UMR 6501, F-49045 Angers, France. Inst Ciencia Mat Barcelona, CSIC, Bellaterra 08193, Spain. RP Hudhomme, P, Univ Angers, Lab Ingn Mol & Mat Organ, CNRS, UMR 6501, 2 Bd Lavoisier, F-49045 Angers, France. TC 18 PD MAY 14 PY 2001 VL 42 IS 20 BP 3447 EP 3450 UT ISI:000168507100013 ER PT J AU Geffard, A Amiard-Triquet, C Amiard, JC Mouneyrac, C TI Temporal variations of metallothionein and metal concentrations in the digestive gland of oysters (Crassostrea gigas) from a clean and a metal-rich site SO BIOMARKERS DE metallothionein; biomarker; metallic pollution; oyster; translocation ID NATURAL FACTORS SALINITY; MACOMA-BALTHICA; PACIFIC OYSTER; CADMIUM; EXPOSURE; PROTEINS; BIVALVE; CD; CONTAMINATION; POPULATIONS AB The concentrations of metallothionein (MT) in bivalves, a potential biomarker of metal pollution, are variable according to specific organs, the highest concentrations being encountered in the digestive glands of oysters. Thus, the present study has been focused on this organ with a view to validate the use of MT as a biomarker in the field, the temporal changes of metal and metallothionein concentrations have been examined from March to October 1997 in the digestive gland of resident oysters from a clean site (Bay of Bourgneuf, France) and a metal-rich site, the Gironde estuary which has been shown as the most CD-contaminated marine area in France but is also enriched with Cu and Zn. Moreover, oysters from the clean site have been translocated to the Gironde estuary over the same period. Taking into account all the samples collected over the 7 months of the study, MT concentrations in the digestive gland were positively correlated with weight whereas metal levels were negatively correlated with weight. However, considering monthly samples including specimens from both sites (resident or translocated oysters), a positive correlation was shown between MT and metal concentrations in autumn (September and October) but not in spring ans summer. These findings limit the interest of using the digestive gland of oysters as the preferred tissue for the determination of MT concentration as a biomarker. The alternative use of gills should be considered. C1 CNRS, Fac Pharm, SMAB, ISOMer,Serv Ecotoxicol, F-44322 Nantes 3, France. IRFA, Dept Sci Vie & Terre, Lab Ecol Anim, F-49100 Angers, France. RP Geffard, A, CNRS, Fac Pharm, SMAB, ISOMer,Serv Ecotoxicol, GDR1117,2 Rue Houssiniere,BP 92208, F-44322 Nantes 3, France. TC 18 PD MAR-APR PY 2001 VL 6 IS 2 BP 91 EP 107 UT ISI:000167969900001 ER PT J AU Barriere, Y Verite, R Brunschwig, P Surault, F Emile, JC TI Feeding value of corn silage estimated with sheep and dairy cows is not altered by genetic incorporation of Bt176 resistance to Ostrinia nubilalis SO JOURNAL OF DAIRY SCIENCE DE corn silage; transgenic corn; milk production; milk composition ID BACILLUS-THURINGIENSIS; MAIZE PLANTS; EFFICIENCY AB A genetically modified Bt176 corn hybrid (Rh208Bt)-providing control of European corn borer damage-and the conventional isogenic hybrid (Rh208)-harvested as whole plant silage-were evaluated in three separate feeding trials to verify that the in vivo feeding value was substantially equivalent among modified and conventional hybrids. In the first trial, after a week of preexperiment, two sets of six Texel sheep, housed in digestibility crates, were fed silage sources of Rh208 and Rh208Bt; hybrids, and silage of three additional control varieties of low, intermediate, and high feeding value (Rh289, Adonis, and Adonis bm3) for 1 wk. Feed offered to sheep was adjusted to maintenance requirements based on metabolic body weight. Agronomic and biochemical traits were similar among the Rh208 and Rh208Bt; hybrids. Organic matter digestibility (67.1 and 67.6%), crude fiber digestibility (52.9 and 54.2%), and neutral detergent fiber digestibility (50.2 and 49.0%) were not significantly different among Rh208 and Rh208Bt hybrids. In the second trial, two sets of 24 Holstein cows were fed silage from Rh208 and Rh208Bt corn hybrids for 13 wk, 9 wk after calving, and including 2 wk of preexperiment. Fat-corrected milk yield (31.3 and 31.4 kg/d), protein content (31.7 and 31.6 g/kg) and fat content (36.7 and 37.0 g/kg) in milk of dairy cows were unaffected by hybrid source. Body weight gains of cattle were not different. However, intake was significantly higher in cows fed Rh208Bt silage. In the third trial, five midlactation multiparous Holstein cows were successively fed the silage from Rh208 and Rh208Bt corn hybrids 2 or 3 wk. Data were considered only for the last week of each period. There were no significant effects on protein fractions, fatty acid composition, or coagulation properties of milk between Rh208 and Rh208Bt fed cattle. Cattle and sheep can perform equally well with a conventional or a genetically modified Bt176 corn silage. C1 INRA, Unite Genet & Ameliorat Plantes Fourrageres, F-86600 Lusignan, France. INRA, Unite Rech Vache Laitiere, F-35590 St Gilles, France. Inst Elevage, F-49006 Angers, France. RP Barriere, Y, INRA, Unite Genet & Ameliorat Plantes Fourrageres, F-86600 Lusignan, France. TC 17 PD AUG PY 2001 VL 84 IS 8 BP 1863 EP 1871 UT ISI:000170470700014 ER PT J AU Venisse, JS Gullner, G Brisset, MN TI Evidence for the involvement of an oxidative stress in the initiation of infection of pear by Erwinia amylovora SO PLANT PHYSIOLOGY ID INDUCED HYPERSENSITIVE REACTION; TOBACCO CELL-SUSPENSIONS; ACTIVE OXYGEN PRODUCTION; LIPID-PEROXIDATION; DISEASE RESISTANCE; HYDROGEN-PEROXIDE; SALICYLIC-ACID; BACTERIA; HARPIN; BURST AB Involvement of an oxidative burst, usually related to incompatible plant/pathogen interactions leading to hypersensitive reactions, was investigated with Erwinia amylovora, the causal agent of fire blight of Maloideae subfamily of Rosaceae, in interaction with pear (Pyrus communis; compatible situation) and tobacco (Nicotiana tabacum; incompatible situation). As expected, this necrogenic bacterium induced in tobacco a sustained production of superoxide anion, lipid peroxidation, electrolyte leakage, and concomitant increases of several antioxidative enzymes (ascorbate peroxidases, glutathion reductases, glutathion-S-transferases, and peroxidases), in contrast to the compatible pathogen Pseudomonas syringae pv tabaci, which did not cause such reactions. In pear leaves, however, inoculations with both the disease- and the hypersensitive reaction-inducing bacteria (E. amylovora and P. syringae pv tabaci, respectively) resulted in superoxide accumulation, lipid peroxidation, electrolyte leakage, and enzyme induction at similar rates and according to equivalent time courses. The unexpected ability of E. amylovora to generate an oxidative stress even in compatible situation was linked to its functional hrp (for hypersensitive reaction and pathogenicity) cluster because an Hrp secretion mutant of the bacteria did not induce any plant response. It is suggested that E. amylovora uses the production of reactive oxygen species as a tool to provoke host cell death during pathogenesis to invade plant tissues. The bacterial exopolysaccharide could protect this pathogen against the toxic effects of oxygen species since a non-capsular mutant of E. amylovora induced locally the same responses than the wild type but was unable to further colonize the plant. C1 Univ Angers, Inst Natl Hort, Inst Natl Rech Agron, Unite Mixte Rech Pathol Vegetale, F-49071 Beaucouze, France. Inst Natl Rech Agron, Unite Ameliorat Especes Fruitieres & Ornementales, F-49071 Beaucouze, France. RP Brisset, MN, Univ Angers, Inst Natl Hort, Inst Natl Rech Agron, Unite Mixte Rech Pathol Vegetale, F-49071 Beaucouze, France. TC 17 PD APR PY 2001 VL 125 IS 4 BP 2164 EP 2172 UT ISI:000168215200063 ER PT J AU Grolleau, F Stankiewicz, M Birinyi-Strachan, L Wang, XH Nicholson, GM Pelhate, M Lapied, B TI Electropysiological analysis of the neurotoxic action of a funnel-web spider toxin, delta-Atracotoxin-Hv1a, on insect voltage-gated Na+ channels SO JOURNAL OF EXPERIMENTAL BIOLOGY DE insect neurone; vertebrate neurone; Na+ channel; spider neurotoxin; atracotoxin; funnel-web spider; Hadronyche versuta ID SCORPION ALPHA-TOXIN; ADULT AMINERGIC NEURONS; HIGH-AFFINITY BINDING; AMINO-ACID SEQUENCE; SODIUM-CHANNELS; RAT-BRAIN; LETHAL NEUROTOXIN; ATRAX-ROBUSTUS; DELTA-ATRACOTOXINS; RECEPTOR-SITE AB The effects of delta -ACTX-Hv1a, purified from the venom of the funnel-web spider Hadronyche versuta, were studied on the isolated giant axon and dorsal unpaired median (DUM) neurones of the cockroach Periplaneta americana under current- and voltage-clamp conditions using the double oil-gap technique for single axons and the patch-clamp technique for neurones, In parallel, the effects of the toxin were investigated on the excitability of rat dorsal root ganglion (DRG) neurones. In both DRG and DUM neurones, delta -ACTX-Hv1a induced spontaneous repetitive firing accompanied by plateau potentials. However, in the case of DUM neurones, pIateau action potentials were facilitated when the membrane was artificially hyperpolarized, In cockroach giant axons, delta -ACTX-Hv1a also produced plateau action potentials, but only when the membrane was pre-treated with 3-4 diaminopyridine, Under voltage-clamp conditions, delta -ACTX-Hv1a specifically affected voltage-gated Naf channels in both axons and DUM neurones. Both the current/voltage and conductance/ voltage curves of the delta -ACTX-Hv1a-modified inward current were shifted 10 mV to the left of control curves. In the presence of delta -ACTX-Hv1a, steady-state Na+ channel inactivation became incomplete, causing the appearance of a non-inactivating component at potentials more positive than -40mV, The amplitude of this non-inactivating component was dependent on the holding potential, From this study, it is concluded that, in insect neurones, delta -ACTX-Hv1a mainly affects Na+ channel inactivation by a mechanism that differs slightly from that of scorpion alpha -toxins. C1 Univ Angers, RCI, EA 2647,UPRES, Lab Neurophysiol,UFR Sci, F-49045 Angers, France. Nicholas Copernicus Univ, Inst Biol, Biophys Lab, PL-87100 Torun, Poland. Univ Technol Sydney, Dept Hlth Sci, Broadway, NSW 2007, Australia. Univ Connecticut, Ctr Hlth, Dept Biochem, Farmington, CT 06032 USA. RP Grolleau, F, Univ Angers, RCI, EA 2647,UPRES, Lab Neurophysiol,UFR Sci, 2 Blvd Lavoisier, F-49045 Angers, France. TC 17 PD FEB PY 2001 VL 204 IS 4 BP 711 EP 721 UT ISI:000167441000010 ER PT J AU Veziers, J Lesourd, M Jollivet, C Montero-Menei, C Benoit, JP Menei, P TI Analysis of brain biocompatibility of drug-releasing biodegradable microspheres by scanning and transmission electron microscopy SO JOURNAL OF NEUROSURGERY DE biocompatibility; drug delivery; neurotrophic factor; biodegradable microsphere; neurodegenerative disease; stereotaxis; rat ID NERVE GROWTH-FACTOR; POLY(D,L-LACTIDE-CO-GLYCOLIDE) MICROSPHERES; POLYMER MICROSPHERES; CORTICAL-LESIONS; NGF; IMPLANTATION; DELIVERY; MICROPARTICLES; NEURONS AB Object. Stereotactic ally guided implantation of biodegradable microspheres is a promising strategy for delivery of neurotrophic factors in a precise and spatially defined brain area. The goal in this study was to show the biocompatibility of poly (D,L,lactide-co-glycolide) microspheres with brain tissue at the ultrastructural level and to analyze the three-dimensional (3D) ultrastructure after intrastriatal implantation of these microparticles. Methods. Scanning and transmission electron microscopy were used to study the microspheres and their environment after implantation in an inert material (gelatin) and in the rat striatum. Observations were made at different time periods, ranging from 24 hours to 2 months postimplantation. Conclusions. The progressive degradation of the microspheres, with vacuolization, deformation, and shrinkage, was well visualized. This degradation was identical in microspheres implanted in the inert material and in the rat brain tissue, independent of the presence of macrophages. The studies preformed in the striatum permitted the authors to demonstrate the structural integrity of axons in contact with microspheres, confirming the biocompatibility of the polymer. Furthermore, scanning electron microscopy showed the preservation of the 3D ultrastructure of the striatum around the microparticles. These microparticles, which can be stereotactically implanted in functional areas of the brain and can release neurotrophic factors, could represent, for some indications, an alternative to gene therapy. C1 CHU Angers, Serv Neurochirurg, F-49033 Angers 01, France. Univ Angers, Fac Pharm, INSERM, Equipe Rech & Innovat Technol & Methodol 0104, Angers, France. Univ Angers, Fac Med, Serv Commun Microscopie Electron, Angers, France. RP Menei, P, CHU Angers, Serv Neurochirurg, F-49033 Angers 01, France. TC 16 PD SEP PY 2001 VL 95 IS 3 BP 489 EP 494 UT ISI:000170894600018 ER PT J AU Besbes, M Trippe, G Levillain, E Mazari, M Le Derf, F Perepichka, IF Derdour, A Gorgues, A Salle, M Roncali, J TI Rapid and efficient post-polymerization functionalization of poly(3,4-ethylenedioxythiophene) (PEDOT) derivatives on an electrode surface SO ADVANCED MATERIALS ID CONDUCTING POLYMERS; CONJUGATED POLYMERS; POLYPYRROLE FILMS; BUILDING-BLOCKS; TETRATHIAFULVALENE; ELECTROSYNTHESIS; POLYTHIOPHENES; CHEMISTRY; ELECTROPOLYMERS; CENTERS C1 Univ Angers, CNRS, UMR 6501, F-49045 Angers, France. Univ Oran Es Senia, Lab Synth Organ Appl, Oran, Algeria. RP Salle, M, Univ Angers, CNRS, UMR 6501, 2 Bd Lavoisier, F-49045 Angers, France. TC 16 PD AUG 16 PY 2001 VL 13 IS 16 BP 1249 EP 1252 UT ISI:000170902600007 ER PT J AU Boudebs, G Sanchez, F Troles, J Smektala, F TI Nonlinear optical properties of chalcogenide glasses: comparison between Mach-Zehnder interferometry and Z-scan techniques SO OPTICS COMMUNICATIONS DE chalcogenide glasses; Mach-Zehnder interferometer; nonlinear refractive index; Fourier transform; third-order susceptibility ID BEAM AB To explore the potentialities of chalcogenide glasses for ultrafast optical switching, the nonlinear refractive indices of different compositions are measured. Several binary glasses as well as ternary ones have been characterized. Nonlinear measurements has been performed through a pump/probe experiment using a Mach-Zehnder interferometer coupled to a CCD camera. Nonlinear refractive index as high as 12 x 10(-18) m(2)/W have been obtained in the ternary (Ge-Se-As) system. A comparison with the nonlinear coefficients obtained with the Z-scan technique is performed, Results show that, although the order of magnitude is the same, a systematic discrepancy exists, in particular for high nonlinear absorption for which the relative error reaches up to 50%. We show that this is a consequence of the use of simplified formulas derived in the Z-scan technique. Indeed, a detailed analysis demonstrates that both techniques perfectly agree when a higher-order development is used for the determination of the nonlinear coefficient from the Z-scan technique. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, UMR CNRS 6163, Lab Proprietes Opt & Mat & Applicat, F-49045 Angers 01, France. Univ Rennes 1, UMR CNRS 6512, Lab Verres & Ceram, F-35042 Rennes, France. RP Boudebs, G, Univ Angers, UMR CNRS 6163, Lab Proprietes Opt & Mat & Applicat, 2 Blvd Lavoisier, F-49045 Angers 01, France. TC 15 PD DEC 1 PY 2001 VL 199 IS 5-6 BP 425 EP 433 UT ISI:000172338700015 ER PT J AU Coutant, R Rouleau, S Despert, F Magontier, N Loisel, D Limal, JMA TI Growth and adult height in GH-treated children with nonacquired GH deficiency and idiopathic short stature: The influence of pituitary magnetic resonance imaging findings SO JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM ID HORMONE DEFICIENCY; FINAL HEIGHT; PREPUBERTAL CHILDREN; PUBERTAL DEVELOPMENT; DIAGNOSIS; CHILDHOOD; THERAPY; STALK; TRANSECTION; POPULATION AB We analyzed the final height of 146 short children with either nonacquired GH deficiency or idiopathic short stature. Our purpose was 1) to assess growth according to the pituitary magnetic resonance imaging findings in the 63 GH-treated children with GH deficiency and 2) to compare the growth of the GH-deficient patients with normal magnetic resonance imaging (n = 48) to that of 32 treated and 51 untreated children with idiopathic short stature (GH peak to provocative tests >10 mug/liter). The mean GH dose was 0.44 IU/kg.wk (0.15 mg/kg-wk), given for a mean duration of 4.6 yr. Among the GH-deficient children, 15 had hypothalamic-pituitary abnormalities (stalk agenesis), all with total GH deficiency (GH peak <5 g/liter). They were significantly shorter and younger at the time of diagnosis than those with normal magnetic resonance imaging, had better catch-up growth (+2.7 +/- 0.9 vs. +1.3 +/- 0.8 SD score; P < 0.01), and reached greater final height (-1.1 +/- 1.0 vs. -1.7 +/- 1.0 SD score; P < 0.05). Among patients with normal magnetic resonance imaging, there was no difference in catch-up growth and final height between partial and total GH deficiencies. GH-deficient subjects with normal magnetic resonance imaging and treated and untreated patients with idiopathic short stature had comparable auxological characteristics, age at evaluation, and target height. Although they had different catch-up growth (+1.3 +/- 0.8, +0.9 +/- 0.6, and +0.7 +/- 0.9 SD score, respectively; P < 0.01, by ANOVA), these patients reached a similar final height (-1.7 +/- 1.0, -2.1 +/- 0.8, and -2.1 +/- 1.0 SD score, respectively; P = 0.13). Pituitary magnetic resonance imaging findings show the heterogeneity within the group of nonacquired GH deficiency and help to predict the response to GH treatment in these patients. The similarities in growth between the GH-deficient children with normal magnetic resonance imaging and those with idiopathic short stature suggest that the short stature in the former subjects is at least partly due to factors other than GH deficiency. C1 Univ Hosp, Dept Pediat, F-49000 Angers, France. Univ Hosp, Dept Radiol, F-49000 Angers, France. Univ Hosp, Dept Pediat, F-37000 Tours, France. RP Coutant, R, Univ Hosp, Dept Pediat, 4 Rue Larrey, F-49000 Angers, France. TC 15 PD OCT PY 2001 VL 86 IS 10 BP 4649 EP 4654 UT ISI:000171755400016 ER PT J AU Leclerc, A Landre, MF Chastang, JF Niedhammer, I Roquelaure, Y CA Study Grp Repetitive Work TI Upper-limb disorders in repetitive work SO SCANDINAVIAN JOURNAL OF WORK ENVIRONMENT & HEALTH DE carpal tunnel syndrome; epicondylitis; longitudinal study; psychosocial factors; work characteristics; wrist tendinitis ID CARPAL-TUNNEL SYNDROME; MEAT-PACKING PLANT; MUSCULOSKELETAL DISORDERS; PSYCHOSOCIAL FACTORS; GENERAL-POPULATION; UPPER EXTREMITY; METAL-INDUSTRY; RISK-FACTORS; MANUAL WORK; TENOSYNOVITIS AB Objectives A longitudinal study was conducted to determine the predictability of personal and occupational factors with respect to the incidence of upper-limb disorders in occupations requiring repetitive work. Methods A sample of 598 workers in five activity sectors completed a self-administered questionnaire; the workers were examined by an occupational health physician in 1993-1994 and 3 years later. Three disorders were considered, carpal tunnel syndrome (CTS), lateral epicondylitis and wrist tendinitis. Results The results of this longitudinal study indicated that the following three sets of risk factors independently affect the incidence of upper-limb disorders: (i) biomechanical constraints, (ii) psychosocial factors, and (iii) personal factors. The combination of risk factors differed between CTS, lateral epicondylitis, and wrist tendinitis. The presence of psychosomatic problems was a strong predictor of wrist tendinitis. Social support at work was also associated with the incidence of wrist tendinitis. The presence of depressive symptoms and other upper-limb disorders predicted the first occurrence of lateral epicondylitis. Age was associated only with epicondylitis. The results were consistent with those concerning the role of forceful movements of the elbow for epicondylitis and confirmed the role of forceful movements for CTS. Conclusions This study considered different sets of risk factors simultaneously with a longitudinal approach, in a population with a high level of occupational exposure. The results indicate that three sets of risk factors independently affect the incidence of upper-limb disorders. In addition to biomechanical constraints, psychosocial and personal factors play a role. C1 Hop Natl St Maurice, INSERM, U88, F-94415 St Maurice, France. CHU Angers, Serv Pathol Profess, Angers, France. RP Leclerc, A, Hop Natl St Maurice, INSERM, U88, 14 Rue val Osne, F-94415 St Maurice, France. TC 15 PD AUG PY 2001 VL 27 IS 4 BP 268 EP 278 UT ISI:000170892000008 ER PT J AU Broderick, GA Walgenbach, RP Maignan, S TI Production of lactating dairy cows fed alfalfa or red clover silage at equal dry matter or crude protein contents in the diet SO JOURNAL OF DAIRY SCIENCE DE alfalfa silage; red clover silage; nonprotein N ID DEGRADATION; DIGESTION; FORAGE; MICROORGANISMS; PROTEOLYSIS; PERFORMANCE; NITROGEN; ACIDS; CORN AB Two Latin square trials, using 21 or 24 multiparous lactating Holstein cows, compared the feeding value of red clover and alfalfa silages harvested over 2 yr. Red clover silages averaged 2 percentage units lower in crude protein (CP) and more than 2 percentage units lower in neutral detergent fiber and acid detergent fiber than did alfalfa silage. In trial 1, diets were formulated to 60% dry matter (DM) from alfalfa, red clover silage, or alfalfa plus red clover silage (grown together); CP was adjusted to about 16.5% by adding soybean meal, and the balance of dietary DM was from ground high moisture ear corn. Nonprotein N in red clover and alfalfa-red clover silages was 80% of that in alfalfa silage. Although DM intake was 2.5 and 1.3 kg/d lower on red clover and alfalfa plus red clover, yield of milk and milk components was not different among diets. In trial 2, four diets containing rolled high moisture shelled corn were formulated to 60% DM from alfalfa or red clover silage, or 48% DM from alfalfa or red clover silage plus 12% DM from corn silage. The first three diets contained 2.9% soybean meal, and the red clover-corn silage diet contained 5.6% soybean meal; the 60% alfalfa diet contained 18.48 CP, and the other three diets averaged 16.5% CP. Nonprotein N in red clover silage was 62% of that in alfalfa silage. Intake of DM was about 2 (no corn silage) and 1 kg/d (plus corn silage) lower on red clover. Yield of milk and milk components was not different among the first three diets; however, yields of milk, total protein, and true protein were higher on red clover-corn silage with added soybean meal. Replacing alfalfa with red clover improved feed and N efficiency and apparent digestibility of DM, organic matter, neutral detergent fiber, acid detergent fiber, and hemicellulose in both trials. Net energy of lactation computed from animal performance data was 18% greater in red clover than alfalfa. Data on milk and blood urea and N efficiency suggested better N utilization on red clover. C1 USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. Ecole Super Agr, F-49007 Angers, France. RP Broderick, GA, USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr W, Madison, WI 53706 USA. TC 15 PD JUL PY 2001 VL 84 IS 7 BP 1728 EP 1737 UT ISI:000169847100020 ER PT J AU Mirallie, E Leclair, MD de Lagausie, P Weil, D Plattner, V Duverne, C DeWint, A Podevin, G Heloury, Y TI Laparoscopic adrenalectomy in children SO SURGICAL ENDOSCOPY-ULTRASOUND AND INTERVENTIONAL TECHNIQUES DE adrenalectomy; pheochromocytoma; ganglioneuroma; laparoscopy; lomboscopy ID OPEN POSTERIOR ADRENALECTOMY; VONHIPPEL-LINDAU-DISEASE; PHEOCHROMOCYTOMA; MANAGEMENT; PARAGANGLIOMAS; SURGERY AB Background: The aim of this study was to describe the indications and results of laparoscopic adrenalectomy in children. Methods: This retrospective multicenter study included six children (four boys, two girls) aged 2-16 years (mean, 9.5), Three children had hypertension. In three cases, the adrenal mass was discovered incidentally. The tumors were bilateral in two children, right-sided in two cases, and left-sided in two cases. The mean tumor size was 4 cm (range, 1-7). Each child underwent MIBG scintigraphy and MRI: before the operation. Results: Four right and two left adrenal glands were resected by laparoscopy (transperitoneal), and two left glands were resected by retroperitoneoscopy. Two conversions were necessary for two left adrenal glands tone retroperitoneoscopy, one laparoscopy). Two partial resections and six adrenalectomies were performed. Histological examination of the tumors revealed two ganglioneuromas, one neuroblastoma, and five pheochromocytomas (two bilateral). One child had an involved node (pheochromocytoma). Both bilateral pheochromocytomas had von Hippel-Lindau disease. There was no morbidity. Mean hospitalstay was 6 days. Postoperative evaluation at 1 month was normal in all children. Conclusion: The indications for laparoscopic adrenalectomy in children are benign tumors and pheochromocytomas. In these cases, laparoscopic adrenalectomy is feasible and safe, even in cases of pheochromocytoma. C1 CHU Nantes, Hotel Dieu, Dept Pediat Surg, F-44093 Nantes 1, France. Hop Robert Debre, Dept Pediat Surg, F-75019 Paris, France. CHU Angers, F-66093 Angers 1, France. RP Plattner, V, CHU Nantes, Hotel Dieu, Dept Pediat Surg, F-44093 Nantes 1, France. TC 15 PD FEB PY 2001 VL 15 IS 2 BP 156 EP 160 UT ISI:000167663800012 ER PT J AU Barrat, JA Blichert-Toft, J Nesbitt, RW Keller, F TI Bulk chemistry of Saharan shergottite Dar al Gani 476 SO METEORITICS & PLANETARY SCIENCE ID ND ISOTOPIC SYSTEMATICS; MARTIAN METEORITES; SIDEROPHILE ELEMENTS; MANTLE; MARS; DIFFERENTIATION; LEW88516; SR; CRYSTALLIZATION; GEOCHEMISTRY AB We report on major and trace element analyses obtained by, respectively, inductively coupled plasma-atomic emission spectrometry (ICP-AES) and inductively coupled plasma-mass spectrornetry (ICP-MS) of three different aliquots of the new Saharan shergottite Dar al Gani (DaG) 476. The new analyses are in excellent agreement with previous data (Zipfel et al., 2000). Ba, Sr and U abundances, together with the presence of carbonate, suggest that the sample has been significantly weathered. Three rare earth element (REE) patterns (normalized to CI) determined on three different aliquots of the sample all show similar shapes. The heavy REEs are flat with a slight depletion at the heavy end and a strong depletion from Dy to Pr. All of the patterns display an upturn to La which we interpret as being caused by the introduction of a terrestrial component. Taking the terrestrial contamination into account, this study demonstrates that DaG 476 is one of the most depleted of the shergottites, and, just like Queen Alexandra Range (QUE) 94201 (Dreibus et al., 1996), displays very low Zr/Hf ratios. It appears that the Zr/Hf ratios of shergottites are not uniform, and have been significantly fractionated by martian mantle processes. C1 Univ Angers, Fac Sci, F-49045 Angers, France. Ecole Normale Super Lyon, Lab Sci Terre, CNRS, UMR 5570, F-69364 Lyon 7, France. Univ Southampton, Southampton Oceanog Ctr, Southampton SO14 3ZH, Hants, England. UJF, CNRS, UMR 5025, F-38400 St Martin Dheres, France. RP Barrat, JA, Univ Angers, Fac Sci, 2 Bd Lavoisier, F-49045 Angers, France. TC 15 PD JAN PY 2001 VL 36 IS 1 BP 23 EP 29 UT ISI:000167652600004 ER PT J AU Nguyen, L Niccoli-Sire, P Caron, P Bastie, D Maes, B Chabrier, G Chabre, O Rohmer, V Lecomte, P Henry, JF Conte-Devolx, B CA French Calcitonin Tumors Study Grp TI Pheochromocytoma in multiple endocrine neoplasia type 2: a prospective study SO EUROPEAN JOURNAL OF ENDOCRINOLOGY ID MEDULLARY-THYROID CARCINOMA; PENTAGASTRIN STIMULATION TEST; RET PROTOONCOGENE MUTATIONS; ADRENAL-SPARING SURGERY; MEN 2A; HEREDITARY PHEOCHROMOCYTOMA; PLASMA METANEPHRINES; LINDAU-DISEASE; DIAGNOSIS; CALCITONIN AB Objective: The aim of this prospective study is to update our knowledge of the chronology of pheochromocytoma occurrence in multiple endocrine neoplasia type 2 (MEN 2), and to better manage MEN 2 patients after the genetic diagnosis. Design: Eighty-seven non-index gene carrier MEN 2 patients were included in this prospective study: 84 patients with MEN 2A (from 52 families) and 3 with MEN 2B (from 3 families), Methods: Medullary thyroid carcinoma (MTC) was diagnosed by measuring plasma calcitonin in basal conditions or after pentagastrin stimulation. The search for pheochromocytoma consisted of clinical evaluation, 24 h determination of urinary catecholamines and adrenal imaging. The mean age at genetic diagnosis of MEN 2 was 14.0 +/- 7.0 years, the mean duration for the follow-up was;7.6 +/- 2.8 pears, Results: All 87 patients had a MTC detected at the same time as the genetic diagnosis was made. Urinary catecholamine measurements led to the diagnosis of pheochromocytoma and a combination of imaging techniques enabled the correct localization of both unilateral or bilateral adrenal involvement. Pheochromocytoma was detected simultaneously with MTC in only seven patients, and seven others were detected throughout the follow-up. Of the 14 patients with pheochromocytoma, 11 had bilateral involvement: nine were initially bilateral and two became so during follow-up. Conclusion: This study demonstrates that in MEN 2, MTC is the lesion which appears earliest. Pheochromocytoma develops later during the evolution of the disease, and necessitates regular clinical and biological monitoring throughout follow-up. Determination of urinary and/or plasma catecholamines and metanephrines should be performed to detect pheochromocytoma. Imaging techniques lead to the detection of both unilateral and bilateral pheochromocytoma, thus making video-assisted laparoscopic adrenalectomy possible. C1 Hop Enfants La Timone, Serv Endocrinol, F-13385 Marseille 05, France. Hop Enfants La Timone, Serv Chirurg Endocrinienne, F-13385 Marseille, France. Hop Rangueil, Serv Endocrinol, Toulouse, France. Inst Claudius Regaud, Ctr Reg Lutte Canc, Toulouse, France. Inst Jean Godinoi, Ctr Reg Lutte Canc, F-51056 Reims, France. Hop Hautepierre, Serv Endocrinol, Strasbourg, France. Hop Michallon, Serv Endocrinol, Grenoble, France. CHU Angers, Serv Endocrinol, Angers, France. Hop Bretonneau, Serv Endocrinol, Tours, France. RP Conte-Devolx, B, Hop Enfants La Timone, Serv Endocrinol, F-13385 Marseille 05, France. TC 15 PD JAN PY 2001 VL 144 IS 1 BP 37 EP 44 UT ISI:000166848100006 ER PT J AU Hideur, A Chartier, T Brunel, M Louis, S Ozkul, C Sanchez, F TI Generation of high energy femtosecond pulses from a side-pumped Yb-doped double-clad fiber laser SO APPLIED PHYSICS LETTERS ID MODE-LOCKING AB We present a passively mode-locked side-pumped Yb-doped double-clad fiber laser, emitting around 1.05 mum. Polarization additive-pulse mode-locking technique is used. Mode locking is self-starting and produces 670 fs pulses after compression with a grating pair inserted in the cavity. The energy per pulse is 24 nJ. (C) 2001 American Institute of Physics. C1 Univ Rouen, UMR Coria 6614, Grp Opt & Optron, F-76821 Mont St Aignan, France. Univ Angers, UMR 6136, Lab POMA, F-49045 Angers 01, France. RP Hideur, A, Univ Rouen, UMR Coria 6614, Grp Opt & Optron, F-76821 Mont St Aignan, France. TC 14 PD NOV 19 PY 2001 VL 79 IS 21 BP 3389 EP 3391 UT ISI:000172204300003 ER PT J AU Hideur, A Chartier, T Brunel, M Salhi, M Ozkul, C Sanchez, F TI Mode-lock, Q-switch and CW operation of an Yb-doped double-clad fiber ring laser SO OPTICS COMMUNICATIONS DE double-clad fiber laser; nonlinear polarization rotation; mode locking; Q-switching ID POLARIZATION; DYNAMICS AB We investigate the dynamics of a high power unidirectional Yb-doped fiber ring laser with an intracavity polarizer placed between two halfwave plates. We show that, depending on the respective orientation of the phase plates, the laser operates in different regimes: CW, Q-switched, mode locked, or unstable. The passive method exposed here allows us to choose the operating regime of the laser by a simple rotation of a halfwave plate. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, UMR 6136, Lab POMA, F-49000 Angers, France. Univ Rouen, UMR 6614, G2O, CORIA, F-76821 Mont St Aignan, France. RP Sanchez, F, Univ Angers, UMR 6136, Lab POMA, 2 Bd Lavoisier, F-49000 Angers, France. TC 14 PD OCT 15 PY 2001 VL 198 IS 1-3 BP 141 EP 146 UT ISI:000171571900021 ER PT J AU Gorsky, A Nekrasov, N Rubtsov, V TI Hilbert schemes, separated variables, and D-branes SO COMMUNICATIONS IN MATHEMATICAL PHYSICS ID INTEGRABLE SYSTEMS; HAMILTONIAN-SYSTEMS; INSTANTONS; EQUATIONS; BUNDLES AB We explain Sklyanin's separation of variables in geometrical terms and construct it for Hitchin and Mukai integrable systems. We construct Hilbert schemes of points on T*Sigma for Sigma = C, C* or elliptic curve, and on C-2/Gamma and show that their complex deformations are integrable systems of Calogero-Sutherland-Moser type. We present the hyperkahler quotient constructions for Hilbert schemes of points on cotangent bundles to the higher genus curves, utilizing the results of Hurtubise, Kronheimer and Nakajima. Finally we discuss the connections to physics of D-branes and string duality. C1 Inst Theoret & Expt Phys, Moscow 117259, Russia. Harvard Univ, Lyman Lab Phys, Cambridge, MA 02138 USA. Univ Angers, Dept Math, F-49045 Angers, France. Inst Mittag Leffler, Djursholm, Sweden. Inst Hautes Etud Sci, F-91440 Bures Sur Yvette, France. RP Gorsky, A, Inst Theoret & Expt Phys, Moscow 117259, Russia. TC 14 PD SEP PY 2001 VL 222 IS 2 BP 299 EP 318 UT ISI:000171175200005 ER PT J AU Charrier, JP Tournel, C Michel, S Comby, S Jolivet-Reynaud, C Passagot, J Dalbon, P Chautard, D Jolivet, M TI Differential diagnosis of prostate cancer and benign prostate hyperplasia using two-dimensional electrophoresis SO ELECTROPHORESIS DE two-dimensional polyacrylamide gel electrophoresis; prostate-specific antigen; prostate cancer; benign prostate hyperplasia; diagnosis ID 2-DIMENSIONAL ELECTROPHORESIS; MOLECULAR-FORMS; ANTIGEN; SERUM; ALPHA-1-ANTICHYMOTRYPSIN; ADENOCARCINOMA; COMPLEX AB Prostate specific antigen (PSA) is a protease which is characteristic of the prostate. it is widely used as a serum marker for the early diagnosis of prostate cancer (PCa). Nevertheless, for concentrations between 4 and 10 ng/mL, PSA does not enable PCa to be distinguished from benign diseases, such as benign prostate hyperplasia (BPH). In sera, the use of a ratio between free PSA (PSA uncomplexed with protease inhibitor) and total PSA (free PSA and PSA bound to alpha-1 anti-chymotrypsin) enables the "gray zone" to be reduced, but an important proportion of patients are still wrongly classed. Using two-dimensional electrophoresis, we demonstrated using 52 PCa and 40 BPH well-documented clinical cases that BPH sera show a significantly greater percentage of low-molecular-weight free PSA elements (IwPSA) than PCa sera. In our study, the use of a ratio between IwPSA and standard free PSA enables the correct diagnosis of 100% of PCa and 82.5% of BPH cases as against when 73.1% and 42.5% respectively were correctly diagnozed using the total PSA and the free/total PSA ratio. This important finding may be related to differences in the mechanism secreting PSA from the prostate into the bloodstream. We have shown how a tissue marker may be turned into a powerful tumor marker by events probably unrelated to its expression. C1 BioMerieux SA, Dept Rech & Dev, Unite Immunoessais, F-69280 Marcy Letoile, France. BioMerieux SA, Dept Rech & Dev, Unite Biomath & Biostat, F-69280 Marcy Letoile, France. ENS, CNRS, UMR 103, BioMerieux, Lyon, France. CHU Angers, Serv Urol, F-49033 Angers, France. RP Charrier, JP, BioMerieux SA, Dept Rech & Dev, Unite Immunoessais, F-69280 Marcy Letoile, France. TC 14 PD MAY PY 2001 VL 22 IS 9 BP 1861 EP 1866 UT ISI:000169298900030 ER PT J AU Ribba, AS Hilbert, L Lavergne, JM Fressinaud, E Boyer-Neumann, C Ternisien, C Juhan-Vague, I Goudemand, J Girma, JP Mazurier, C Meyer, D TI The arginine-552-cysteine (R1315C) mutation within the Al loop of von Willebrand factor induces an abnormal folding with a loss of function resulting in type 2A-like phenotype of von Willebrand disease: study of 10 patients and mutated recombinant von Willebrand factor SO BLOOD ID PLATELET GLYCOPROTEIN-IB; FACTOR A1 DOMAIN; ALANINE-SCANNING MUTAGENESIS; IIB VONWILLEBRAND DISEASE; BINDING DOMAIN; MONOCLONAL-ANTIBODIES; CRYSTAL-STRUCTURE; GENE DEFECTS; FACTOR VWF; IDENTIFICATION AB The study identified 10 patients from 6 families with prolonged bleeding time, decreased von Willebrand factor (vWF) ristocetin cofactor activity (RCoF) to vWF:Ag (antigen) ratio, and reduced ristocetin-induced platelet agglutination as well as ristocetin- or botrocetin-induced binding of plasma vWF to platelet glycoprotein Ib (GpIb), In addition, all patients showed a decrease of intermediate-molecular-weight (intermediate-MW) and high-molecular-weight (HMW) multimers of vWF, In the heterozygous state, a cysteine-to-threonine (C --> T) transversion was detected at nucleotide 4193 of the VWF gene of all patients and lead to the arginine (R)522C substitution in the Al loop of vWF mature subunit (R1315C in the preprovWF), By in vitro mutagenesis of full-length complementary DNA (cDNA) of vWF and transient expression in COS-7 cells, the mutated C552 recombinant vWF (C552rvWF) was found to exhibit decreased expression, abnormal folding, and lack of intermediate-MW and HMW multimers. In addition, direct binding of botrocetin to C552rvWF, as well as ristocetin- and botrocetin-induced binding of C552rvWF to GpIb, was markedly decreased. Although being localized in an area of the Al loop of vWF where most of the type 2B mutations that induce a gain-of-function have been identified, the R552C mutation induces a PA-like phenotype with a decrease of intermediate-MW and HMW multimers as well as a loss-of-function of vWF in the presence of either ristocetin or botrocetin. (C) 2001 by The American Society of Hematology. C1 Hop Bicetre, INSERM U143, F-94276 Le Kremlin Bicetre, France. CHU Lille, Hematol Lab, F-59037 Lille, France. CHU Lille, Lab Francais Fractionnement & Biotechnol, F-59037 Lille, France. CHU Lille, Hematol Lab, F-59037 Lille, France. CHU Angers, Hemostase Lab, Angers, France. CHU, Hematol Lab, Marseille, France. RP Ribba, AS, Hop Bicetre, INSERM U143, 84 Rue Gen Leclerc, F-94276 Le Kremlin Bicetre, France. TC 14 PD FEB 15 PY 2001 VL 97 IS 4 BP 952 EP 959 UT ISI:000166867200022 ER PT J AU Chappard, D Chennebault, A Moreau, M Legrand, E Audran, M Basle, MF TI Texture analysis of X-ray radiographs is a more reliable descriptor of bone loss than mineral content in a rat model of localized disuse induced by the Clostridium botulinum toxin SO BONE DE botulinum toxin; disuse osteoporosis; texture analysis; X-rays; image analysis; fractal geometry ID CANCELLOUS BONE; TRABECULAR BONE; PHOTON-ABSORPTIOMETRY; FRACTAL DIMENSION; IMAGE-ANALYSIS; SPACE-FLIGHT; OSTEOPOROSIS; MASS; HISTOMORPHOMETRY; IMMOBILIZATION AB Botulism is a generalized paralyzing disease caused by the toxin of Clostridium botulinum (BTX), The toxin acts 3-4 days after injection by blocking the release of acetylcholine to the muscle, Six Wistar rats received a 2-U injection of BTX in the right quadriceps. Six rats were similarly injected with saline and were used as control, Paralysis of the quadriceps was obtained 4-5 days after the injection. Animals were killed 4 weeks after the BTX injection. The bone mineral content (BMC) was measured by dual-energy X-ray absorptiometry (DXA) on the femur and tibia, No side-to-side difference was observed for BMC on the whole tibia and femur in the BTX group. When subregions were selected in the bones, a significant decrease in BMC was obtained on the proximal tibia (-17,4 +/- 2.5%, p < 0.02), No significant difference could be observed on the proximal or distal femur, nor on the diaphyseal shafts, Numeric X-rays were done and a region of interest was transferred to an image analyzer, The texture of the trabecular bone was analyzed by the run length and fractal methods (skyscrapers and blanket). Significant differences were obtained on the proximal tibia for all methods except with fractal skyscrapers. On the distal femur, significant differences were obtained with the run length method, and the skyscrapers and the blanket method in the vertical direction. No differences were obtained with any method on the tibia and femur from control animals. Bone is a highly anisotropic material and its architecture at the microscopic level is conditioned by strains. The trabecular pattern differs in the proximal tibia than in the distal femural, Depending on the trabecular anisotropy, the algorithms can be more or less pertinent. BTX induced a significant bone loss on the bony subparts that are directly influenced by disuse. Texture analysis of X-ray images can reveal differences that were not evidenced by naked eyes, However, a combination of several methods appears necessary to appreciate the bone loss. (C) 2001 by Elsevier Science me. All rights reserved. C1 CHU Angers, Lab Histol Embryol, Angers, France. Fac Med, Lab Histol Embryol, LHEA, F-49045 Angers, France. CHU Angers, Serv Rhumatol, Angers, France. RP Chappard, D, Fac Med, Lab Histol Embryol, LHEA, F-49045 Angers, France. TC 14 PD JAN PY 2001 VL 28 IS 1 BP 72 EP 79 UT ISI:000166677000010 ER PT J AU Berezhiani, VI Skarka, V Aleksic, NB TI Dynamics of localized and nonlocalized optical vortex solitons in cubic-quintic nonlinear media SO PHYSICAL REVIEW E ID PARA-TOLUENE SULFONATE; SELF-TRAPPED BEAMS; SCHRODINGER-EQUATION; RING FORMATION; KERR AB The nonlinear dynamics of laser beams carrying phase singularity in media with cubic-quintic nonlinearity changing from self-focusing to self-defocusing is examined. A novel kind of stable nonlocalized optical vortices appears in such media as well as localized vortex solitons. Linear stability analysis and numerical simulations show the stability of localized vortices only in the defocusing region. C1 Univ Tokyo, Grad Sch Frontier Sci, Tokyo 1130033, Japan. Univ Angers, Lab POMA, CNRS, UMR 6136, F-49045 Angers 1, France. RP Berezhiani, VI, Univ Tokyo, Grad Sch Frontier Sci, Hongo 7-3-1, Tokyo 1130033, Japan. TC 13 PD NOV PY 2001 VL 64 IS 5 PN Part 2 BP art. no. EP 057601 AR 057601 UT ISI:000172407100155 ER PT J AU Chappard, D Legrand, E Haettich, N Chales, G Auvinet, B Eschard, JP Hamelin, JP Basle, MF Audran, M TI Fractal dimension of trabecular bone: comparison of three histomorphometric computed techniques for measuring the architectural two-dimensional complexity SO JOURNAL OF PATHOLOGY DE bone histomorphometry; fractal geometry; bone architecture; male osteoporosis; bone structure; fractal dimension ID CANCELLOUS BONE; ILIAC CREST; ORGANIZATION; OSTEOPOROSIS; CONNECTIVITY; GEOMETRY; PATTERN; IMAGES; VOLUME AB Trabecular bone has been reported as having two-dimensional (2-D) fractal characteristics at the histological level, a finding correlated with biomechanical properties. However, several fractal dimensions (D) are known and computational ways to obtain them vary considerably. This study compared three algorithms on the same series of bone biopsies, to obtain the Kolmogorov, Minkowski-Bouligand, and mass-radius fractal dimensions. The relationships with histomorphometric descriptors of the 2-D trabecular architecture were investigated. Bone biopsies were obtained from 148 osteoporotic male patients. Bone volume (BV/TV), trabecular characteristics (Tb.N, Tb.Sp, Tb.Th), strut analysis, star volumes (marrow spaces and trabeculae), interconnectivity index, and Euler-Poincare number were computed. The box-counting method was used to obtain the Kolmogorov dimension (D-k), the dilatation method for the Minkowski-Bouligand dimension (D-MB), and the sandbox for the mass-radius dimension (D-MR) and lacunarity (L). Logarithmic relationships were observed between BV/TV and the fractal dimensions. The best correlation was obtained with D-MR and the lowest with D-MB. Lacunarity was correlated with descriptors of the marrow cavities (ICI, star volume, Tb.Sp). Linear relationships were observed among the three fractal techniques which appeared highly correlated. A cluster analysis of all histomorphometric parameters provided a tree with three groups of descriptors: for trabeculae (Tb.Th, strut); for marrow cavities (Euler, ICI, Tb.Sp, star volume, L); and for the complexity of the network (Tb.N and the three D's). A sole fractal dimension cannot be used instead of the classic 2-D descriptors of architecture; D rather reflects the complexity of branching trabeculae. Computation time is also, an important determinant when choosing one of these methods. Copyright (C). 2001 John Wiley & Sons, Ltd. C1 CHU, LHEA,GEROM, Lab Hist Embryol, Fac Med, F-49045 Angers, France. CHU Angers, GEROM, Serv Rhumatol, F-49033 Angers, France. CHG, Serv Rhumatol, Le Mans, France. CHU, Serv Rhumatol, Rennes, France. CHG, Serv Rhumatol, Laval, France. CHU, Serv Rhumatol, Reims, France. CHG, Serv Rhumatol, La Roche Sur Yon, France. RP Chappard, D, CHU, LHEA,GEROM, Lab Hist Embryol, Fac Med, F-49045 Angers, France. TC 13 PD NOV PY 2001 VL 195 IS 4 BP 515 EP 521 UT ISI:000172057500016 ER PT J AU Tamas, G Marre, M Astorga, R Dedov, I Jacobsen, J Lindholm, A CA Insulin Aspart Study Grp TI Glycaemic control in type I diabetic patients using optimised insulin aspart or human insulin in a randomised multinational study SO DIABETES RESEARCH AND CLINICAL PRACTICE DE insulin aspart; human insulin; glycaemic control; type 1 diabetes ID BLOOD-GLUCOSE CONTROL; INJECTION-MEAL-INTERVAL; DOUBLE-BLIND CROSSOVER; ANALOG LISPRO; INTENSIVE TREATMENT; GLYCEMIC CONTROL; ACTION PROFILE; NPH INSULIN; TRIAL; MANAGEMENT AB Insulin aspart (IAsp), is a rapid-acting analogue of human insulin (HI), for use in the meal related treatment of diabetes mellitus. The degree of glycaemic control achieved by IAsp in comparison with HI after algorithm-driven dose optimisation was tested over 3 months. The prospective, multicentre, randomised, open-label study with parallel groups was performed in 48 centres in 11 countries and included 423 basal-bolus treated patients with Type I diabetes. Main outcome measures were blood glucose control assessed by HbA(1c), nine-point self-monitored blood glucose profiles, insulin dose, quality of life, hypoglycaemia and adverse events. An algorithm-driven increase occurred in the dose and number of daily injections of basal insulin, particularly in the IAsp group. After 12 weeks of treatment, HbA(1c) was significantly lower in IAsp compared to HI treated subjects by 0.17 (95% CI 0.30-0.04) (P < 0.05). Comparison of the blood glucose profiles showed lower blood glucose levels with IAsp after breakfast (mean 8.4 vs 10.1 mmol/I; P < 0.0001) and dinner (8.2 vs 9.3 mmol/l; P < 0.01). There were no differences between treatments in the incidence of hypoglycaemic episodes or in the adverse event profiles. The WHO Diabetes Treatment Satisfaction Questionnaire score for perceived hyperglycaemia was lower with Iasp (P = 0.005), and patients found the insulin aspart treatment more flexible (P = 0.022). The current study underlines the need for optimising the basal insulin regimen in order to take full advantage of the pharmacoodynamics of IAsp. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved. C1 Semmelweis Univ Med, Fac Med, Dept Med 1, Natl Ctr Diabet Care,Diabet Unit, H-1083 Budapest, Hungary. CHU Angers, Angers, France. Hosp Virgen del Rocio, Seville, Spain. Russian Acad Med Sci, Moscow, Russia. Novo Nordisk AS, DK-2880 Bagsvaerd, Denmark. RP Tamas, G, Semmelweis Univ Med, Fac Med, Dept Med 1, Natl Ctr Diabet Care,Diabet Unit, Koranyi Sandor Utca 2A, H-1083 Budapest, Hungary. TC 13 PD NOV PY 2001 VL 54 IS 2 BP 105 EP 114 UT ISI:000172216400005 ER PT J AU Jouad, EM Larcher, G Allain, M Riou, A Bouet, GM Khan, MA Do Thanh, X TI Synthesis, structure and biological activity of nickel(II) complexes of 5-methyl 2-furfural thiosemicarbazone SO JOURNAL OF INORGANIC BIOCHEMISTRY DE Ni(II) complexes; thiosemicarbazone complexes; crystal structures; spectroscopic studies; biological activity ID COPPER(II) COMPLEXES; CRYSTAL-STRUCTURE; METAL-COMPLEXES; N-4-SUBSTITUTED THIOSEMICARBAZONES; MOLECULAR-STRUCTURE AB 5-Methyl 2-furfuraldehyde thiosemicarbazone (M5HFTSC) with nickel(H) leads to three types of complexes: [Ni(M5HFTSC)(2)X-2], [Ni(M5FrSC)(2)] and [Ni(M5FTSC)(2)]. 2DMF. In the first type the ligand remains in thione form, while in the two other, the anionic thiolato form is involved. The species [Ni(M5HFTSC)(2)X-2] has been characterized spectroscopically. The structures of [Ni(M5FrSC)(2)]. 2DMF and [Ni(M5FFSC)(2)] have been solved using X-ray diffraction. Biological studies of [Ni(M5HFTSC)(2)Cl-2] have been carried out in vitro for antifungal activity on human pathogenic fungi, Aspergillus fumigatus and Candida albicans, and in vivo for toxicity on mice. The results are compared to those of the ligand, the metal salt and a similar copper complex [Cu(M5HFTSC)Cl-2]. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, Fac Pharm, F-49045 Angers 01, France. CHU Angers, Grp Etud Interact Hote Parasite, Lab Parasitol Mycol, F-49033 Angers, France. Fac Sci, IMMO, UMR 6501, CNRS, F-49045 Angers, France. Univ Angers, Lab Pharmacol Toxicol, Fac Med & Pharm, F-49100 Angers, France. RP Bouet, GM, Univ Angers, Fac Pharm, 16 Blvd Daviers, F-49045 Angers 01, France. TC 13 PD SEP PY 2001 VL 86 IS 2-3 BP 565 EP 571 UT ISI:000171350800004 ER PT J AU Arnaud, JP Pessaux, P Huten, N De Manzini, N Tuech, JJ Laurent, B Simone, M TI Treatment of hemorrhoids with circular stapler, a new alternative to conventional methods: A prospective study of 140 patients SO JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS ID RANDOMIZED CONTROLLED TRIAL AB BACKGROUND: Surgical hemorrhoidectomy has a reputation for being a painful procedure. The aim of this study was to determine the efficacy and safety of a new procedure for surgical treatment of hemorrhoid disease. STUDY DESIGN: From April 1998 to August 1998, 140 patients (83 men and 57 women) with an average age of 43.8 years (range 19 to 83 years) underwent hemorrhoidectomy using a circular stapler. Operative times, peri- and postoperative complications, mean hospital stay, assessment of the postoperative pain, period of incapacity for work, and functional results were collected. All patients were evaluated at 2 weeks, 2 months, and 18 months after operation. RESULTS: The average length of the operation was 18 minutes (range 8 to 60 minutes). There were no perioperative complications. The postoperative complication rate was 6.4% (n = 9). Mean hospital stay was 36 hours (range 8 to 72 hours). Paracetamol was the only analgesic used. Eighty-three patients (59.3%) required analgesic for less than 2 days, 45 patients (32.1%) between 2 and 7 days, and 12 patients (8.6%) more than 7 days. No patients had anal wound care. One hundred four patients had professions. The period of incapacity for work was less than 5 days for 22 patients (21.1%), between 3 and 7 days for 13 patients (12.5%), between 7 and 14 days for 62 patients (59.6%), and more than 14 days for 7 patients (6.8%). At 18 months, 95.7% of patients were fully satisfied with the results, 3.6% were somewhat satisfied (n = 4), and 0.7% were unsatisfied. CONCLUSIONS: Treatment of hemorrhoids with a circular stapler appears to be safe, effective, and rapid, causing few postoperative complications and minimal postoperative pain. At 18 months, 95.7% of the patients were fully satisfied with the results. (J Am Coll Surg 2001; 193:161-165. (C) 2001 by the American College of Surgeons). C1 CHU Angers, Dept Visceral Surg, F-49033 Angers 01, France. CHU Tours, Hop Trousseau, Tours, France. CHU Strasbourg, Hop Hautepierre, F-67000 Strasbourg, France. RP Arnaud, JP, CHU Angers, Dept Visceral Surg, 4 Rue Larrey, F-49033 Angers 01, France. TC 13 PD AUG PY 2001 VL 193 IS 2 BP 161 EP 165 UT ISI:000170139200007 ER PT J AU Lortholary, A Abadie-Lacourtoisie, S Guerin, O Mege, M De Rauglaudre, G Gamelin, E TI Carcinoma of unknown primary site: 311 cases SO BULLETIN DU CANCER DE carcinoma of unknown origin; metastases ID POORLY DIFFERENTIATED CARCINOMA; COMBINATION-CHEMOTHERAPY REGIMENS; PRIMARY TUMOR SITE; METASTATIC CANCER; UNDIFFERENTIATED CARCINOMA; NATURAL-HISTORY; ADENOCARCINOMA; ORIGIN; EXPERIENCE; IDENTIFICATION AB carcinoma of unknown primary site has been defined as a metastatic disease without known primary site (upon clinical, radiological or endoscopic examination) at the initial therapeutic derision. The incidence of such carcinomas is between 1.6 and 15% of all adult's tumors. The goals of this retrospective and monocentric study were ii the incidence of these carcinoma; 2) the utility to identify the primary site; 3) the efficacy of treatment in terms of survival; and 4) the prognostic factors to optimize strategic choices. Between January 1980 to December 1995, 311 cases were identified; this represents 1.6% of all cases treated in our center Histological analyses of metastases revealed adenocarcinoma: 164 cases (92 males, 72 females; 29 well differentiated, II poorly differentiated and 41 undifferentiated); squamous cell carcinoma: 90 cases (78 males, 12 females); undifferentiated carcinoma: 27 cases (21 males, 6 females); neuro-endocrine tumor: 10 cases; and others: 20 cases. Median age was 61.1 years (30-94). Half of the patients had a PS between 0 and 1. The carcinoma was revealed by only one site of metastases in 35% of the cases (lymph node 72.9%, bone 35.5%, liver 19.4% and lung 165%). The primary carcinoma was found in only 6% of the cases. Median survival of all patients was only 9 months. Multivariate analyses by the Cox method show four positive prognostic factors: sex (female), performance status (PS < 2), histological analyses (squamous cell carcinoma), only one site of metastases. C1 Ctr Paul Papin, F-49033 Angers, France. RP Lortholary, A, Ctr Paul Papin, 2 Rue Moll, F-49033 Angers, France. TC 13 PD JUN PY 2001 VL 88 IS 6 BP 619 EP 627 UT ISI:000169993400011 ER PT J AU Broet, P Romain, S Daver, A Ricolleau, G Quillien, V Rallet, A Asselain, B Martin, PM Spyratos, F CA Grp Oncobiol Federat Natl Ctr Lu TI Thymidine kinase as a proliferative marker: Clinical relevance in 1,692 primary breast cancer patients SO JOURNAL OF CLINICAL ONCOLOGY ID S-PHASE FRACTION; DNA FLOW-CYTOMETRY; PROGNOSTIC FACTORS; ESTROGEN-RECEPTOR; QUALITY-CONTROL; MITOTIC INDEX; CHEMOTHERAPY; CARCINOMA; TUMORS; KI-67 AB Purpose: To assess the prognostic value of thymidine kinase (TK), an enzyme involved in the DNA synthesis salvage pathway, relative to other prognostic factors in primary breast cancer. Patients and Methods: This retrospective study involved 1,692 patients with operable breast cancer heated in six Institutions (median follow-up, 82 months), among the 857 node-negative patients, 135 received adjuvant chemotherapy (fluorouracil, doxorubicin, cyclophosphamide [FAC] or fluorouracil, etoposide, and cisplatin [FEC]), TK war arrayed in cytosal with a quantitative radioenzymatic technique, Disease/specific survival (DSS), local recurrence-free interval (LRI), and distant-relapse-free interval (DRI) were investigated, Results: High TK levels were associated with large tumor rite, high histologic grade, and steroid hormone receptor negativity, Univariate analysis of the entire data set showed that high TK levels were related ta shorter DSS (P < 10(-5)), LRI (P < 10(-3)), and DRI (P < 10(-5)), In time-dependent Cox madels, high TK levels remained an independent predictor of the three outcomes, both In the overall population and in node-negative patients, although ih prognostic value decreased over time. In node-negative patients, the introduction of an interaction term in multivariate analysis suggested that chemotherapy war more efficacious for patients who hold tumors with high TK contents, In node-positive patients, high TK levels were related only to an increased rick of LRI. Conclusion: High TK values am an important rick factor in node-negative patients and seem rp be associated with a beneficial effect of adjuvant FAC or FEC in patients who received adjuvant chemotherapy, The rationale of chemotherapy for patients with slowly proliferating tumors has to be discussed from a risk-benefit point of view. C1 Ctr Rene Huguenin, Lab Oncobiol, F-92210 St Cloud, France. Inst Curie, Paris, France. Ctr Hosp Univ Nord, Assistance Publ Hop Marseille, Marseille, France. Ctr P Papin, Angers, France. Ctr Rene Gauducheau, St Herblain, France. Ctr E Marquis, Rennes, France. Ctr J Godinot, Reims, France. RP Spyratos, F, Ctr Rene Huguenin, Lab Oncobiol, 35 Rue Dailly, F-92210 St Cloud, France. TC 13 PD JUN 1 PY 2001 VL 19 IS 11 BP 2778 EP 2787 UT ISI:000169034000003 ER PT J AU Liu, SG Kreher, D Hudhomme, P Levillain, E Cariou, M Delaunay, J Gorgues, A Vidal-Gancedo, J Veciana, J Rovira, C TI The dumbbell his Diels-Alder adduct between tetramethylidene[4H]tetrathiafulvalene and two C-60 SO TETRAHEDRON LETTERS ID PHOTOINDUCED ELECTRON-TRANSFER; TETRATHIAFULVALENE TTF; BIS-LINKING; PHOTOVOLTAIC CELLS; PI-DONORS; <60>FULLERENE; BUCKMINSTERFULLERENE; FULLERENES; POLYMER; EPR AB Dumbbell C-60-TTF-C-60 was synthesized by a double cycloaddition of C-60 with the unprecedented tetramethylidene[4H]TTF generated in situ from tetrakis(bromomethyl)TTF. (C) 2001 Elsevier Science Ltd. All rights reserved. C1 Univ Angers, Lab Ingn Mol & Mat Organ, UMR CNRS 6501, F-49045 Angers, France. CSIC, Inst Ciencia Mat Barcelona, Bellaterra 08193, Spain. RP Hudhomme, P, Univ Angers, Lab Ingn Mol & Mat Organ, UMR CNRS 6501, 2 Bd Lavoisier, F-49045 Angers, France. TC 13 PD MAY 28 PY 2001 VL 42 IS 22 BP 3717 EP 3720 UT ISI:000168985200013 ER PT J AU Stone, JD Peterson, AP Eyer, J Oblak, TG Sickles, DW TI Neurofilaments are nonessential to the pathogenesis of toxicant-induced axonal degeneration SO JOURNAL OF NEUROSCIENCE DE neurofilaments; acrylamide; 2,5-hexanedione; gamma-diketones; neuropathies; transgenic mice ID AMYOTROPHIC-LATERAL-SCLEROSIS; PERIPHERAL NERVOUS-SYSTEM; 3,4-DIMETHYL SUBSTITUTION; MOLECULAR PATHOGENESIS; ACRYLAMIDE NEUROPATHY; PROTEIN CROSSLINKING; INDUCED REDUCTIONS; HEXANE NEUROPATHY; JAPANESE-QUAILS; MOUSE MODEL AB Axonal neurofilament (NF) accumulations occur before development of symptoms and before other pathological changes among idiopathic neurodegenerative diseases and toxic neuropathies, suggesting a cause-effect relationship. The dependence of symptoms and axonal degeneration on neurofilament accumulation has been tested here in a transgenic mouse model (Eyer and Peterson, 1994) lacking axonal NFs and using two prototypic toxicant models. Chronic acrylamide (ACR) or 2,5-hexanedione exposure resulted in progressive and cumulative increases in sensorimotor deficits. Neurobehavioral tests demonstrated similar expression of neurotoxicity in transgenic (T) mice and their nontransgenic (NT) littermates (containing normal numbers of axonal NFs). Axonal lesions were frequently observed after exposure to either toxicant. Quantitation of ACR-induced lesions demonstrated the distal location of pathology and equal susceptibility of T and NT axons. We conclude that axonal NFs have no effect on neurotoxicity and the pattern of pathology in these mammalian toxic neuropathies, These results also suggest that the role of neurofilament accumulation in the pathogenesis of neurodegenerative diseases requires careful evaluation. C1 Med Coll Georgia, Dept Cellular Biol & Anat, Augusta, GA 30912 USA. McGill Univ, Dept Neurol & Neurosurg, Montreal, PQ H3A 1A1, Canada. McGill Univ, Mol Oncol Grp, Montreal, PQ H3A 1A1, Canada. CHU Angers, INSERM, F-49033 Angers, France. RP Sickles, DW, Med Coll Georgia, Dept Cellular Biol & Anat, Augusta, GA 30912 USA. TC 13 PD APR 1 PY 2001 VL 21 IS 7 BP 2278 EP 2287 UT ISI:000167737100012 ER PT J AU Bouligand, Y Norris, V TI Chromosome separation and segregation in dinoflagellates and bacteria may depend on liquid crystalline states SO BIOCHIMIE DE liquid crystal; DNA segregation; cholesteric phase ID ESCHERICHIA-COLI; DNA INVIVO; REPLICATION; ORGANIZATION; ARRANGEMENT; MESOPHASES; INVITRO; PHASES AB The patterns characteristic of certain liquid crystals called 'twisted nematics' or 'cholesterics' have been observed in thin sections of both dinoflagellates and bacterial chromosomes. These liquid crystals have also been obtained in vitro in concentrated DNA solutions. A large part of DNA in prokaryotic chromosomes forms such a twisted liquid crystal, whilst the remainder consists of lateral loops and is less concentrated. These semi-ordered phases could help chromosome separation to occur during and after DNA replication. We suggest that, owing to chemical differences, one of the two replicated filaments is immiscible with the rest of DNA in this chromosome. This immiscibility occurs in the context of an ordered liquid, with the DNA closely layered by a regular twist, a situation proposed to strongly minimize entangling after replication and hence to facilitate segregation. (C) 2001 Societe francaise de biochimie et biologie moleculaire / Editions scientifiques et medicales Elsevier SAS. C1 Histophys EPHE, F-49100 Angers, France. Univ Rouen, Fac Sci & Tech, CNRS, UPRESA 6037,Lab Proc Integratifs Cellulaires, F-76821 Mt St Aignan, France. Fac Pharm Angers, F-49100 Angers, France. RP Bouligand, Y, Histophys EPHE, 10 Rue A Bocquel, F-49100 Angers, France. TC 13 PD FEB PY 2001 VL 83 IS 2 BP 187 EP 192 UT ISI:000167686800007 ER PT J AU Hudhomme, P Le Moustarder, S Durand, C Gallego-Planas, N Mercier, N Blanchard, P Levillain, E Allain, M Gorgues, A Riou, A TI S-position isomers of BEDT-TTF and EDT-TTF: Synthesis and influence of outer sulfur atoms on the electrochemical properties and crystallographic network of related organic metals SO CHEMISTRY-A EUROPEAN JOURNAL DE BEDT-TTF isomers; crystal engineering; organic materials; sulfur heterocycles; tetrathiafulvalene ID UNSYMMETRICAL ELECTRON-DONOR; CHARGE-TRANSFER SALTS; RADICAL CATIONS; TETRATHIAFULVALENE TTF; CRYSTAL-STRUCTURES; AMBIENT-PRESSURE; INCREASED DIMENSIONALITY; DIATOMIC SULFUR; BUILDING-BLOCKS; PI-DONOR AB The synthesis and characterization of new modified tetrathiafulvalenes (TTF), the S-position isomers of BEDT-TTF and EDT-TTF. are described. The synthetic strategy presented in this work is based on an efficient and unprecedented two-step sequence for the conversion of a vicinal bis(hydroxymethyl) functionality into a disulfide ring. Different routes are discussed in terms of efficiency for the synthesis of the symmetric S-position isomer of BEDT-TTF and that of EDT-TTF. Their electrochemical properties are combined with data obtained from UV/Vis spectroscopy and orbital calculations, and the electronic influence of peripheral sulfur atoms on the neutral and oxidized species is discussed. The introduction of these outer sulfur atoms at the periphery of the TTF core gives rise to specific intermolecular S . . .S interactions in the corresponding organic materials. Crystallographic studies of radical cation salts synthesized upon electrocrystallization clearly showed that the network obtained is dictated by the outer sulfur atoms, which are responsible for a characteristic and unprecedented "windmill" array. C1 Univ Angers, UMR 6501, F-49045 Angers, France. RP Hudhomme, P, Univ Angers, UMR 6501, 2 Blvd Lavoisier, F-49045 Angers, France. TC 12 PD DEC 3 PY 2001 VL 7 IS 23 BP 5070 EP 5083 UT ISI:000172599900009 ER PT J AU Frere, P Boubekeur, K Jubault, M Batail, P Gorgues, A TI Synthesis and electrochemical properties of highly extended and sulfur-rich vinylogs as of tetrathiafulvalene SO EUROPEAN JOURNAL OF ORGANIC CHEMISTRY DE electrochemistry; thiafulvalenes ID CATION-RADICAL SALTS; DONOR MOLECULES; SPACER GROUPS; PI-DONORS; ANALOGS; POLYMERS; TTF AB Using the reactivity of the carbonyl groups of 4,5-diformyl-1,3-dithiol-2-ylidene-substituted ethanals or ethanones with ylides or phosphonate anions, highly extended analogs of TTF 1 and 2 were synthesized by Wittig or Wittig-Horner reactions. Their excellent pi -donor properties, as well as the possibility of obtaining polycationic states, were confirmed by cyclic voltammetry. The structural study of cation-radical salt 1b beta gamma . PF6 reveals a two-dimensional network in the solid state. C1 Univ Angers, UMR 6501, CNRS, F-49045 Angers, France. CNRS, FRE 2068, Lab Sci Mol Interfaces, F-44322 Nantes 03, France. RP Frere, P, Univ Angers, UMR 6501, CNRS, 2 Bd Lavoisier, F-49045 Angers, France. TC 12 PD OCT PY 2001 IS 19 BP 3741 EP 3747 UT ISI:000171454200018 ER PT J AU Guilet, D Seraphin, D Rondeau, D Richomme, P Bruneton, J TI Cytotoxic coumarins from Calophyllum dispar SO PHYTOCHEMISTRY DE Calophyllum dispar; coumarins; Clusiaceae AB Six coumarins have been isolated from the fruits and the stem bark of Calophyllum dispar (Clusiaceae). The structures of these minor components were established by means of spectroscopic analysis, including extensive 2D NMR studies. Some of these coumarins exhibited a significant cytotoxic activity against KB cells. (C) 2001 Elsevier Science Ltd. All rights reserved. C1 UFR Sci Pharmaceut, SONAS, F-49100 Angers, France. UFR Sci, SCAS, F-49045 Angers, France. RP Richomme, P, UFR Sci Pharmaceut, SONAS, 16 Bd Daviers, F-49100 Angers, France. TC 12 PD OCT PY 2001 VL 58 IS 4 BP 571 EP 575 UT ISI:000171360700008 ER PT J AU Michel, JC Riviere, LM Bellon-Fontaine, MN TI Measurement of the wettability of organic materials in relation to water content by the capillary rise method SO EUROPEAN JOURNAL OF SOIL SCIENCE ID PARTICLE-SIZE; REPELLENCE; SOILS AB A new measuring technique based on a capillary rise method has been developed to quantify the wettability of more or less decomposed peats (the main materials used as horticultural growing media) in relation to their water content. A preliminary study of their physico-chemical and biochemical characteristics enabled us to study the variation of their physical properties (particularly their rewetting capacity) in relation to their degree of decomposition. Analysis of these physical properties during two drying and rewetting cycles showed that the greater their degree of decomposition, the greater and more irreversible was their shrinkage. The change from hydrophilicity to hydrophobicity was measured by the increase of the water contact angle during desiccation and by the progressive change from bipolar to apolar surfaces. A strong correlation between the water retention of peats (related to their degree of decomposition) and their free energy (also called hydration energy) at the interface between solid and water DeltaG(SW) is also shown. C1 Univ Angers, INH, INRA, Unite Mixte Rech SAGAH, F-49071 Beaucouze, France. INRA, Unite Bioadhes & Hyg Mat, F-91300 Massy, France. RP Michel, JC, Univ Angers, INH, INRA, Unite Mixte Rech SAGAH, 42 Rue Georges Morel, F-49071 Beaucouze, France. TC 12 PD SEP PY 2001 VL 52 IS 3 BP 459 EP 467 UT ISI:000170903600011 ER PT J AU Sahraoui, B Kityk, IV Hudhomme, P Gorgues, A TI Temperature-pressure anomalies of electrooptic coefficients in C-60-TTF derivatives SO JOURNAL OF PHYSICAL CHEMISTRY B ID C-60-2-THIOXO-1,3-DITHIOLE CYCLOADDUCT; 2-PHOTON ABSORPTION; ORDER-DISORDER; CHEMISTRY AB A sharplike modulation of the linear electrooptic (LEO) coefficient with pressure (p) and temperature (T) has been observed for the first time. The modulation depth of the LEO (r(222)) tenser component in the C-60-TTF (for the He-Ne laser, lambda = 633 nm) was equal to about 2.6%. By varying the applied hydrostatic pressure to up to 15 GPa and the temperature from 4.2 to 25 K, we have unambiguously demonstrated that the observed effect is caused by the incorporation of the TTF moiety. This effect is absent from the unsubstituted fullerenes when the applied hydrostatic pressure is increased to 19 GPa and the temperature varied from 4.2 to 150 K. Norm-conserving self-consistent pseudopotential and molecular dynamics simulations independently reproduce these modulations and attribute them to the electron-vibration interactions associated with the LEO coefficients. The contribution of the interconformational tunneling under the applied pressure and temperature is demonstrated. The data obtained give a possibility for using the discovered effect for contactless measurements of low temperatures and high pressures. C1 Univ Angers, Lab POMA, EP 130, CNRS,UFR Sci, F-49045 Angers, France. Univ Angers, Lab Ingn Mol & Mat Opt, UMR 6501, CNRS, F-49045 Angers, France. Inst Phys WSP, PL-42201 Czestochowa, Poland. RP Kityk, IV, Univ Angers, Lab POMA, EP 130, CNRS,UFR Sci, 2 Blvd Lavoisier, F-49045 Angers, France. TC 12 PD JUL 12 PY 2001 VL 105 IS 27 BP 6295 EP 6299 UT ISI:000169823800004 ER PT J AU Becher, J Brimert, T Jeppesen, JO Pedersen, JZ Zubarev, R Bjornholm, T Reitzel, N Jensen, TR Kjaer, K Levillain, E TI Tetrathiafulvaleno-annelated porphyrins SO ANGEWANDTE CHEMIE-INTERNATIONAL EDITION DE cyclic voltammetry; EPR; spectroscopy; Langmuir-Blodgett films; porphyrinoids; tetrathiafulvalenes ID PARENT SYSTEMS; CHEMISTRY; FILMS C1 Odense Univ, Dept Chem, Odense 5230 M, Denmark. Univ Copenhagen, Nanosci Ctr, Dept Chem, Copenhagen 2100 0, Denmark. Riso Natl Lab, Condensed Matter Phys & Chem Dept, Roskilde 4000, Denmark. Univ Angers, CNRS UMR 6501, F-49045 Angers, France. RP Becher, J, Odense Univ, Dept Chem, Campusvej 55, Odense 5230 M, Denmark. TC 12 PY 2001 VL 40 IS 13 BP 2497 EP + UT ISI:000169706000025 ER PT J AU Courjaret, R Lapied, B TI Complex intracellular messenger pathways regulate one type of neuronal alpha-bungarotoxin-resistant nicotinic acetylcholine receptors expressed in insect neurosecretory cells (dorsal unpaired median neurons) SO MOLECULAR PHARMACOLOGY ID CILIARY GANGLION NEURONS; PROTEIN PHOSPHATASE 2A; HETEROLOGOUS EXPRESSION; PERIPLANETA-AMERICANA; ELECTRICAL-ACTIVITY; LOCUSTA-MIGRATORIA; CHROMAFFIN CELLS; MUSHROOM BODIES; MYZUS-PERSICAE; ION CHANNELS AB Although molecular biology provides new insights into the subunit compositions and the stoichiometries of insect neuronal nicotinic acetylcholine receptors (nAChRs), our knowledge about the phosphorytation/dephosphorylation mechanisms of native neuronal nAChRs is limited. The regulation of alpha -bungarotoxin-resistant nAChRs was studied on dissociated adult dorsal unpaired median neurons isolated from the terminal abdominal ganglion of the cockroach Periplaneta americana, using whole-cell, patch-clamp technique. Under 0.5 muM alpha -bungarotoxin treatment, pressure ejection application of nicotine or acetylcholine onto the cell body induced an inward current exhibiting a biphasic current-voltage relationship. We found that two distinct components underlying the biphasic curve differed in their ionic permeability and pharmacology tone being sensitive to d-tubocurarine, and the other affected only by mecamylamine and alpha -conotoxin Iml). This indicated that two types of alpha -bungarotoxin-resistant nAChRs (named nAChR1 and nAChR2) mediated the nicotinic response. These two components were also differentially sensitive to rundown and intracellular messengers. Intracellular application of 0.1 mM cAMP only increased the current amplitude mediated by nAChR1. Using forskolin (1 muM), W7 and H89, we demonstrated that adenylyl cyclase, sensitive to calcium/calmodulin complex, regulated nAChR1 via a cAMP/cAMP-dependent protein kinase cascade. By contrast, internal cAMP concentration higher than 0.1 mM reduced the current amplitude. This effect, mimicked by high external concentration of forskolin (100 muM) and IBMX, was reversed by okadaic acid, suggesting the implication of a protein phosphatase. Using KN-62, we demonstrated that calmodulin-Kinase II also modulated directly and indirectly nAChR1, via an inhibition of the phosphatase activity. Finally, we reported that phosphorylation/dephosphorylation of nAChR1 strongly affected the action of the widely used neonicotinoid insecticide imidacloprid. C1 Univ Angers, UFR Sci, RCIM,Unite Foramt & Rech Sci, Lab Neurophysiol,UPRES EA 2647, F-49045 Angers, France. RP Lapied, B, Univ Angers, UFR Sci, RCIM,Unite Foramt & Rech Sci, Lab Neurophysiol,UPRES EA 2647, 2 Blvd Lavoisier, F-49045 Angers, France. TC 12 PD JUL PY 2001 VL 60 IS 1 BP 80 EP 91 UT ISI:000169506300009 ER PT J AU Abraham, P Bickert, S Vielle, B Chevalier, EM Saumet, JL TI Pressure measurements at rest and after heavy exercise to detect moderate arterial lesions in athletes SO JOURNAL OF VASCULAR SURGERY ID EXTERNAL ILIAC ARTERY; SYSTOLIC BLOOD-PRESSURE; ARM INDEX; ANKLE; DIAGNOSIS; DISEASE; ENDOFIBROSIS; TREADMILL; CYCLISTS AB Purpose: This study defined how ankle arterial blood pressure measurements should be analyzed for the detection of moderate arterial disease (asymptomatic while walking). We used external iliac artery endofibrosis as a unique model of an isolated moderate arterial lesion, the role of which in exercise-related pain can be surgically proven. Methods: Patients who were ambulatory in our institutional referral center were studied. Brachial pressures, ankle pressures, and heart rate were measured simultaneously on all four limbs at rest and after maximal exercise in 108 healthy athletes and 78 patients (among 89 athletes referred for suspicion of endofibrosis) with confirmed or excluded external iliac endofibrosis. For these 78 patients, we calculated systolic ankle pressure change, ankle/brachial index, and deviation from the ankle/brachial index to heart rate regression line (DAHR) that was defined in the 108 healthy athletes. Results: In patients with endofibrosis, ankle/brachial index and ankle pressure were normal at rest. One minute after exercise, areas (mean +/- SE of area) under the receiver operating characteristics curve for the diagnosis of endofibrosis were 0.91 +/- 0.02, 0.91 +/- 0.03, 0.95 +/- 0.02, and 0.96 +/- 0.02 for ankle pressure, pressure change, ankle/brachial index, and DAHR respectively. For all criteria, area decreased with time in the recovery period. Conclusion: After heavy-load exercise, the ankle/brachial index at minute 1 should be used rather than the systolic ankle pressure value or ankle pressure change as a means of improving the efficacy of the detection of endofibrosis in athletes. A 0.66 value of the index at minute 1 after maximal exercise seems an optimal cutoff point for clinical use, providing a 90% sensitivity rate and 87% specificity rate in the diagnosis of moderate arterial lesions. At rest and after 1 minute of recovery, the ankle/brachial index to heart rate relationship should be considered to be an efficient tool for analyzing the results of pressures measurements and improving detection efficiency. C1 CHU Angers, Lab Physiol & Explorat Vasc, F-49033 Angers 01, France. CHU Angers, Lab Stat Med, F-49033 Angers, France. Hop Edouard Herriot, Serv Chirurg Vasc, Lyon, France. RP Saumet, JL, CHU Angers, Lab Physiol & Explorat Vasc, 4 Rue Larrey, F-49033 Angers 01, France. TC 12 PD APR PY 2001 VL 33 IS 4 BP 721 EP 727 UT ISI:000168027000008 ER PT J AU Abraham, P Fromy, B Merzeau, S Jardel, A Saumet, JL TI Dynamics of local pressure-induced cutaneous vasodilation in the human hand SO MICROVASCULAR RESEARCH DE neural control; skill blood flow; pressure; laser Doppler; microcirculation ID LASER DOPPLER FLUXMETRY; SKIN BLOOD-FLOW; BIOLOGICAL ZERO; GLABROUS SKIN; STIMULATION; MICROCIRCULATION; PLETHYSMOGRAPHY; NOCICEPTORS; CAPSAICIN; AFFERENTS AB We recently demonstrated that a pressure-induced vasodilation results from local nonnociceptive stimulation of the skin of the human hand. We aimed to test the hypothesis that this vasodilation was not a short-lived response to a single type of pressure strain, but could be a widely activated and prolonged protective cutaneous response. We studied the dynamics of pressure-induced vasodilation during various ramp changes in local externally applied pressure using laser Doppler flowmetry. Changes from an adjacent control probe were subtracted from pressure-induced local changes. Following an initial transient decrease, continuous 4.4, 5.6, and 11.1 Pa.s(-1) increases of pressure resulted in a secondary increase of blood flow whose amplitude was maximal for 11.1 Pa.s(-1) (22.9 +/- 12.6% above baseline) (mean +/- SEM). The increase in flow was not noted at 16.7 Pa.s(-1). If the 16.7 Pa.s(-1) ramp pressure increase was interrupted at min 2 or 4, a prolonged vasodilation response was found, but not if it was stopped at min 8. When the 16.7 Pa.s(-1) increasing pressure was returned to zero after 4 min of pressure increase (-8.1 +/- 8.9% before pressure removal), vasodilation occurred and reached a maximum of 26.0 +/- 9.6% at 7 min after removal of pressure (P < 0.05 from baseline). Pressure-induced vasodilation is a slow-responding and transient adaptive phenomenon, initiated by a wide range of pressure changes below the range of noxious stimulation. We propose that this response is a protective mechanism without which certain pressure-associated lesions may develop. (C) 2001 Academic Press. C1 CHU Angers, Lab Physiol & Explorat Vasc, F-49033 Angers 01, France. UFR Sci Pharmaceut, Dept Physiol, F-49045 Angers, France. RP Abraham, P, CHU Angers, Lab Physiol & Explorat Vasc, F-49033 Angers 01, France. TC 12 PD JAN PY 2001 VL 61 IS 1 BP 122 EP 129 UT ISI:000166821900012 ER PT J AU Tuech, JJ Regenet, N Hennekinne, S Pessaux, P Bergamaschi, R Arnaud, JP TI Laparoscopic colectomy for sigmoid diverticulitis in obese and nonobese patients - A prospective comparative study SO SURGICAL ENDOSCOPY-ULTRASOUND AND INTERVENTIONAL TECHNIQUES DE laparoscopy; colon; obese patients; overweight patients; sigmoid diverticulitis; high-risk patients ID ASSISTED COLECTOMY; COLORECTAL SURGERY; MORBIDLY OBESE; CHOLECYSTECTOMY; MULTICENTER; RESECTION AB Background: The aim of this prospective comparative study was to assess the outcome of laparoscopic colectomy for sigmoid diverticulitis in normal-weight, overweight, and obese patients. Methods: From January 1995 to December 2000, all patients (it = 77) undergoing an elective colectomy for sigmoid diverticulitis were enrolled in the study. The patients were divided into three groups: Group I (n = 29) consisted of healthy, normal-weight patients (BMI, 18-24.9); group 2 (n = 27) consisted of overweight patients (BMI, 25.0-29.9); group 3 (n = 21) consisted of obese patients (BMI, 30.0-39.9). Groups 2 and 3 were compared with group 1. Results: Group I was comprised of 13 women and 16 men with a mean age of 58.4 years (range, 37-78); group 2. was comprised of 13 women and 14 men with a mean age of 55.2 years (range, 31-83); group 3, was comprised of 13 women and 14 men with a mean age of 54.1 years (range, 33-86). There was no difference among the three groups in ASA classification, postoperative length of hospital stay, or inpatient rehabilitation. The operating time did not differ for groups I and 2 (187 vs 210 min, p = 0.6), but it was shorter in group I than in group 3 (187 vs 247 min, p = 0.003). The conversion rate was similar for all three groups: 17.2% in group 1, 14.8% in group 2, and 19% in group 3. The postoperative period during which parenteral analgesics were required did not differ between groups I and 2 (5.7 vs 7.7 days, p = 0.1), but it was longer for group 3 (8.5 days, p = 0.03). The morbidity rate was similar for all three groups: 17.2% in group 7, 14.8% in group 2, and 19% in group 3. There were no perioperative deaths. Conclusions: Data from the present study suggest that laparoscopic colectomy for sigmoid diverticulitis coin be applied safely in overweight and obese patients. C1 Angers Univ Hosp, Dept Digest Surg, F-49000 Angers, France. RP Tuech, JJ, Angers Univ Hosp, Dept Digest Surg, 4 Rue Larrey, F-49000 Angers, France. TC 11 PD DEC PY 2001 VL 15 IS 12 BP 1427 EP 1430 UT ISI:000172658900012 ER PT J AU Ziadi, S Poupard, P Brisset, MN Paulin, JP Simoneau, P TI Characterization in apple leaves of two subclasses of PR-10 transcripts inducible by acibenzolar-S-methyl, a functional analogue of salicylic acid SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY DE Malus domestica L. cv. Golden Delicious; PR-10 genes; acibenzolar-S-methyl; defence mechanisms ID PATHOGENESIS-RELATED PROTEINS; BIRCH POLLEN ALLERGEN; SYSTEMIC ACQUIRED-RESISTANCE; BET-V-1 GENE FAMILY; DISEASE RESISTANCE; MAJOR ALLERGEN; CDNA CLONING; EXPRESSION; PROMOTER; MAL-D-1 AB This study is focusing on the identification of PR-10 genes expressed in leaves of Malus domestica L, cv. Golden Delicious treated with acibenzolar-S-methyl (ASM), a functional analogue of salicylic acid. The complete sequence of four cDNAs (named AP1 to AP4) was determined and the corresponding genes were grouped into two subclasses: APa including AP1 and AP4, and APb regrouping AP2 and AP3. The nucleotide sequence of AP2 Plus upstream regulating sequences was found nearly identical to the previously described gene 1'pr10*Md.a. By contrast, members of the APa subclass were considered as yet undescribed apple PR-10 genes. Results of gene expression show that the transcripts of the AN subclass strongly accumulated from 20 up to 120 h after ASM application in treated leaves and systemically in Upper untreated leaves 120 h after ASM treatment. Significantly lower levels of induction were recorded with transcripts of the APb subclass. Using immunoblot analysis, two polypeptides of 17 and 18 kDa, respectively, were detected in leaves 48 h after ASM application. Immunodetcction performed on leaf sections allowed the localization of proteins in the Palisade parenchyma and the vascular tissues. The results su cst that the apple PR-10 genes, in particular those of the APa subclass, which arc strongly inducible by ASM, could play a role in local and systemic defences. (C) 2001 Academic Press. C1 Univ Angers, UFR Sci, UMR Pathol Vegetale 077, Lab Microbiol Vegetale, F-49045 Angers 01, France. Ctr Angers, INRA, Unite Pathol Vegetale & Phytobacteriol, F-49071 Beaucouze, France. RP Poupard, P, Univ Angers, UFR Sci, UMR Pathol Vegetale 077, Lab Microbiol Vegetale, 2 Blvd Lavoisier, F-49045 Angers 01, France. TC 11 PD JUL PY 2001 VL 59 IS 1 BP 33 EP 43 UT ISI:000172193300005 ER PT J AU Audran, M Chappard, D Legrand, E Libouban, H Basle, MF TI Bone microarchitecture and bone fragility in men: DXA and histomorphometry in humans and in the orchidectomized rat model SO CALCIFIED TISSUE INTERNATIONAL DE histomorphometry; architectecture; male osteoporosis; orchidectomy; fracture risk ID TRABECULAR BONE; MALE OSTEOPOROSIS; CANCELLOUS BONE; ARCHITECTURE; CONNECTIVITY AB In men, the risk of fragility fractures increases as bone mineral declines but there is an overlap in the bone mineral density (BMD) measurements between patients with and those without fractures. Biomechanical competence of trabecular (Th) bone depends on the amount of bone and on microarchitecture. We have developed new histomorphometric methods for evaluating microarchitecture on histological sections. These methods were used in the orchidectomized male rat (ORX - a model of hypogonadism-induced osteoporosis) and on transiliac bone biopsies performed in male osteoporotic patients. ORX rats were studied at 2, 4, 8, and 16 weeks post-ORX. Bone mineral content (BMC) was reduced at 16 weeks. Trabecular bone volume (BV/TV) was significantly decreased from the 4(th) week. Differences in the sensitivity of the methods were found. Fractal dimension was modified as early as 2 weeks and appeared the most potent descriptor of Tb disorganization. The architectural changes in this model mimic those observed in hypogonadic men. We examined the relationships among BMD, micro-architecture, and vertebral fracture in 108 men with lumbar osteopenia (T-score <-2.5). At least one vertebral fracture was observed in 62 patients and none in 46 patients. After adjusting for age, body mass index (BMI), and BMD, there was no significant difference between the two groups in BV/TV, Tb.Th, and Star volume. In contrast, the mean values of ICI and Tb.Sp were significantly higher whereas Tb.N and nodes were lower in patients With vertebral fracture. Logistic regression analysis showed that ICI, strut analysis, and Tb.N were significant predictors of the presence of vertebral fracture: odds ratios for an alteration of 1 SD ranged from 1.7 for nodes to 3.2 for ICI. These results strongly suggest that bone Tb microarchitecture is a major and independent determinant of vertebral fracture in men with osteoporosis. C1 CHU Angers, Serv Rhumatol, F-49033 Angers, France. CHU Angers, Lab Histol Embryol, LHEA, GEROM, F-49045 Angers, France. Fac Med, F-49045 Angers, France. RP Audran, M, CHU Angers, Serv Rhumatol, F-49033 Angers, France. TC 11 PD OCT PY 2001 VL 69 IS 4 BP 214 EP 217 UT ISI:000171955000011 ER PT J AU Chartier, T Hideur, A Ozkul, C Sanchez, F Stephan, GM TI Measurement of the elliptical birefringence of single-mode optical fibers SO APPLIED OPTICS ID PRINCIPAL STATES; POLARIZATION DISPERSION; BEAT-LENGTH; LASERS; TRANSMISSION; CABLE AB The polarization properties of single-mode optical fibers are theoretically modeled with use of the Jones formalism. The fiber is described as an elliptical birefringent plate. The properties predicted by this model are discussed and lead to the development of a simple experimental method to extract the parameters that describe areal fiber. A magneto-optic method that measures the beat length of the fiber is also presented and gives a more complete description of the fiber. The validity of the model is then clearly established. Finally, the wavelength dependence of the parameters characteristic of the fiber is experimentally investigated. (C) 2001 Optical Society of America. C1 Univ Rouen, Grp Opt & Optron, UMR 6614, F-76821 Mont St Aignan, France. Univ Angers, Lab Proprietes Opt Mat & Applicat, F-49045 Angers 01, France. Ecole Natl Super Sci Appl & Technol, Lab Optron, F-22305 Lannion, France. RP Chartier, T, Univ Rouen, Grp Opt & Optron, UMR 6614, Complexe Rech Interprofess Aerothermochim,Pl Emil, F-76821 Mont St Aignan, France. TC 11 PD OCT 20 PY 2001 VL 40 IS 30 BP 5343 EP 5353 UT ISI:000171613800007 ER PT J AU Ewers, FW Ameglio, T Cochard, H Beaujard, F Martignac, M Vandame, M Bodet, C Cruiziat, P TI Seasonal variation in xylem pressure of walnut trees: root and stem pressures SO TREE PHYSIOLOGY DE fructose; glucose; Juglans regia; nutrient uptake; sucrose; temperature; xylem sap ID MAPLE ACER-SACCHARUM; HYDRAULIC CONDUCTIVITY; SAP FLOW; EMBOLISM; WINTER; DYSFUNCTION; CAVITATION; SUGAR; ALTERNATION; RECOVERY AB Measurements of air and soil temperatures and xylem pressure were made on 17-year-old orchard trees and on 5-year-old potted trees of walnut (Juglans regia L.). Cooling chambers were used to determine the relationships between temperature and sugar concentration ([glucose] + [fructose] + [sucrose], GFS) and seasonal changes in xylem pressure development. Pressure transducers were attached to twigs of intact plants, root stumps and excised shoots while the potted trees were subjected to various temperature regimes in autumn, winter and spring. Osmolarity and GFS of the xylem sap (apoplast) were measured before and after cooling or warming treatments. In autumn and spring, xylem pressures of up to 160 kPa were closely correlated with soil temperature but were not correlated with GFS in xylem sap. High root pressures were associated with uptake of mineral nutrients from soil, especially nitrate. In autumn and spring, xylem pressures were detected in root stumps as well as in intact plants, but not in excised stems. In contrast, in winter, 83 % of the xylem sap osmolarity in both excised stems and intact plants could be accounted for by GFS, and both GFS and osmolarity were inversely proportional to temperature. Plants kept at 1.5 degreesC developed positive xylem pressures up to 35 kPa, xylem sap osmolarities up to 260 mosmol l(-1) and GFS concentrations up to 70 g l(-1). Autumn and spring xylem pressures, which appeared to be of root origin, were about 55% of the theoretical pressures predicted by osmolarity of the xylem sap. In contrast, winter pressures appeared to be of stem origin and were only 7% of the theoretical pressures, perhaps because of a lower stem water content during winter. C1 Univ Clermont Ferrand, INRA, UMR PIAF, F-63039 Clermont Ferrand 2, France. Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. Univ Angers, UMR SAGAH, INH, Ctr INRA Angers, F-49071 Beaucouze, France. Unite Agron, F-63039 Clermont Ferrand 2, France. RP Ameglio, T, Univ Clermont Ferrand, INRA, UMR PIAF, Site INRA Crouelle,234 Ave Brezet, F-63039 Clermont Ferrand 2, France. TC 11 PD SEP PY 2001 VL 21 IS 15 BP 1123 EP 1132 UT ISI:000171356300004 ER PT J AU Thibault, F Calil, B Buldyreva, J Chrysos, M Hartmann, JM Bouanich, JP TI Experimental and theoretical CO2-Ar pressure-broadening cross sections and their temperature dependence SO PHYSICAL CHEMISTRY CHEMICAL PHYSICS ID HIGH-RESOLUTION SPECTROSCOPY; POTENTIAL-ENERGY SURFACES; DER-WAALS COMPLEXES; ROTATIONAL RELAXATION; SCATTERING THEORY; LINE-SHAPES; Q-BRANCH; STATES; COLLISIONS; ARGON AB Line broadening coefficients of the CO2-Ar fundamental nu (3) band are obtained experimentally and theoretically for temperatures from 120 to 765 K. The experimental results are obtained by Fourier-transform infrared spectroscopy. Theoretical values are provided via quantum-mechanical (close coupling and coupled states) approaches as well as semiclassical (improved Smith-Giraud-Cooper and Robert Bonamy) methods. The most up-to-date CO2-Ar ab initio intermolecular potential (J. M. Hutson, A. Ernesti, M. M. Law, C. F. Roche and R. J. Wheatley, J. Chem. Phys., 1996,105, 9130) is employed for all calculations. For all the temperatures probed, theoretical values are found to be in a rather good agreement with experiment. In addition, the Raman Q(j) line broadening coefficients and the application of the random phase approximation are presented. C1 Univ Rennes 1, CNRS, UMR 6627, F-35042 Rennes, France. Univ Angers, CNRS, UMR 6136, F-49045 Angers 01, France. Univ Paris 11, LPPM, CNRS, UPR 3361, F-91405 Orsay, France. RP Thibault, F, Univ Rennes 1, CNRS, UMR 6627, Campus Beaulieu, F-35042 Rennes, France. TC 11 PD SEP 15 PY 2001 VL 3 IS 18 BP 3924 EP 3933 UT ISI:000171313700009 ER PT J AU Debenay, JP Tsakiridis, E Soulard, R Grossel, H TI Factors determining the distribution of foraminiferal assemblages in Port Joinville Harbor (Ile d'Yeu, France): the influence of pollution SO MARINE MICROPALEONTOLOGY DE foraminifera; bioindicators; pollution; harbor; Atlantic; France ID BENTHIC FORAMINIFERA; LIVING FORAMINIFERA; BRITISH-COLUMBIA; ESTUARINE; POPULATIONS; SEDIMENTS; RESPONSES; INLET AB Port Joinville harbor is located on an island. Thus, it receives only a few freshwater inputs, contrary to most of the areas where the influence of pollution on foraminiferal assemblages has been studied. The pollution in the harbor mainly results from the boats, including cleaning, painting and outfall of oil and motor-fuel. A total of 59 sediment samples was collected at three sampling periods (November-December 1997, May 1998 and September 1998). These samplings were supplemented by the study of algal flora and macrobenthos and by the study of water circulation by means of six stations where water was collected every hour during a tide cycle. Contaminants were analyzed in the last series of 17 samples. Total assemblages were used for this study. This choice is explained and discussed. This study shows that the main factor that determines the distribution of foraminiferal species in Port Joinville harbor is the geographical position. The correlation that occurs between heavy metals and the silt and clay fraction makes it difficult to determine whether sediment characteristics or pollution have the stronger influence on foraminiferal assemblages, except in areas heavily affected by pollution. Polluted sediments, near the careening areas, are indicated by the tolerant pioneer species Cribroelphidium excavatum and Haynesina germanica. The growth of epiphytic species depends on the presence of algae and their distribution may be favored by local conditions such as the constant immersion of the supports in the wet dock. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, Dept Geol, UPRES EA 2644, F-49045 Angers, France. LEBIM, Ker Chalon, F-85350 Ile Dyeu, France. CQEL, DDE de la Vendee, Serv Maritime, F-85100 Les Sables Olonne, France. IFREMER, F-44311 Nantes, France. RP Debenay, JP, Univ Angers, Dept Geol, UPRES EA 2644, 2 Bd Lavoisier, F-49045 Angers, France. TC 11 PD SEP PY 2001 VL 43 IS 1-2 BP 75 EP 118 UT ISI:000171413100004 ER PT J AU Lynch, MA Duval, O Sukhanova, A Devy, J MacKay, SP Waigh, RD Nabiev, I TI Synthesis, biological activity and comparative analysis of DNA binding affinities and human DNA topoisomerase I inhibitory activities of novel 12-alkoxy-benzo[c]phenanthridinium salts SO BIOORGANIC & MEDICINAL CHEMISTRY LETTERS ID BENZOPHENANTHRIDINE ALKALOIDS; FAGARONINE; SPECTROSCOPY; ETHOXIDINE; NITIDINE; DRUGS AB New antitumor 12-alkoxy-benzo[c]phenanthridinium derivatives were obtained in high yields through multistep syntheses. Analysis of DNA binding and human DNA topoisomerase I inhibitory activities demonstrates that new compounds. combining 2, 6, and 12 substitutions, interact strongly with DNA and exhibit important topoisomerase I inhibition. The cytotoxicities against solid tumor cell lines are also determined and compared with those for fagaronine and ethoxidine. (C) 2001 Elsevier Science Ltd All rights reserved. C1 UFR Pharm, Lab SONAS, F-49100 Angers, France. Strathclyde Inst Biomed Sci, Dept Pharmaceut Sci, Glasgow G4 0NR, Lanark, Scotland. Univ Reims, UFR Pharmacie, IFR Biomol 53, EA Interact Mol & Cellulaires Cancerol 3306, F-51100 Reims, France. RP Duval, O, UFR Pharm, Lab SONAS, 16 Blvd Duviers, F-49100 Angers, France. TC 11 PD OCT 8 PY 2001 VL 11 IS 19 BP 2643 EP 2646 UT ISI:000171174300019 ER PT J AU Pessaux, P Regenet, N Tuech, JJ Rouge, C Bergamaschi, R Arnaud, JP TI Laparoscopic versus open cholecystectomy - A Prospective comparative study in the elderly with acute cholecystitis SO SURGICAL LAPAROSCOPY ENDOSCOPY & PERCUTANEOUS TECHNIQUES DE acute cholecystitis; elderly; high-risk patients; laparoscopic cholecystectomy ID MANAGEMENT AB The aim of this prospective comparative study was to determine the feasibility and the efficacy of laparoscopic cholecystectomy for acute cholecystitis in patients older than 75 years of age and to compare the results with those of open cholecystectomy. From January 1992 to December 1999. 139 patients older than 75 years of age underwent cholecystectomy for acute cholecystitis. The two groups of patients with cholecystolithiasis included 50 patients who underwent laparoscopic cholecystectomy (group 1) and 89 patients who underwent open cholecystectomy (group 2). Group I consisted of 30 women and 20 men., with a mean age of 81.9 years (range. 75-98). Group 2 consisted of 51 women and 38 men, with a mean age of 81.9 years (range, 75-93). There was no difference in the American Society of Anesthesiologists classification in both groups. The length of the surgery (103.3 vs. 149.7 minutes), postoperative length of stay (7.7 vs. 12.7 days), and inpatient rehabilitation (15 vs. 42 patients) were significantly shorter in group I than in group 2. The postoperative morbidity rate was not different between the groups. There was no mortality in group 1, but four patients died in group 2 (P = 0.29). The conversion rate was 32% (n = 16) in group 1. In summary, laparoscopic cholecystectomy in elderly patients with acute cholecystitis is safe and effective. Laparoscopic cholecystectomy in elderly patients restores them to the best possible quality of life with the lowest cost to them physiologically. C1 CHU Angers, Dept Visceral Surg, F-49033 Angers 01, France. RP Pessaux, P, CHU Angers, Dept Visceral Surg, 4 Rue Larrey, F-49033 Angers 01, France. TC 11 PD AUG PY 2001 VL 11 IS 4 BP 252 EP 255 UT ISI:000170548100005 ER PT J AU Pouchot, J Ruperto, N Lemelle, I Sommelet, D Grouteau, E David, L Duquesne, A Deslandre, CJ Paut, IK Pillet, P Goumy, L Barbier, C Guyot, MH Mazingue, F Laloum, SG Fischbach, M Quartier, P Guyot, C Jean, S Le Gall, E Plouvier, E Bost, M de Lumley, L LePlege, A Larbre, JP Guillemin, F Coste, J Landgraf, JM Prieur, AM CA Paediat Rheumatology Int Trials Or; French Study Grp Quality Life Rheu TI The French version of the Childhood Health Assessment Questionnaire (CHAQ) and the Child Health Questionnaire (CHQ) SO CLINICAL AND EXPERIMENTAL RHEUMATOLOGY DE French Childhood Health Assessment Questionnaire (CHAQ); French Child Health Questionnaire (CHQ); cross cultural adaptation and psychometric; evaluation; health related quality of life; juvenile idiopathic arthritis (JIA); healthy children ID ARTHRITIS AB We report the results of the cross cultural adaptation and validation into the French language of two health status instruments. The Childhood Health Assessment Questionnaire (CHAQ) is a disease specific instrument that measures functional ability in daily living activities in children with juvenile idiopathic arthritis (JIA). The Child Health Questionnaire (CHQ) is a generic health related quality of life instrument designed to capture the physical and psychosocial well-being of children independently from the underlying disease. Five hundred children were enrolled including 306 patients with JIA classified into systemic (23%), polyarticular (22%), extended oligoarticular (25%), and persistent oligoarticular (30%) subtypes, and 194 healthy children. Both instruments were reliable with intra-class correlation (ICC) coefficients for the test-retest procedure of 0.91 for the CHAQ, and 0.87 and 0.89 for the physical and psychosocial summary scores of CHQ, respectively. Agreement between parents and children evaluated for the CHAQ was high with an ICC of 0.89 for the disability index; weighted kappa coefficients for the 8 domains ranged from 0.61 to 0.72. Convergent validity, was demonstrated by significant correlations with the JIA core set of variables (physician and parent global assessment, scores for active joints and joints with limited range of motion, erythrocyte sedimentation rate)for both instruments. Both CHAQ and CHQ discriminated between healthy and JIA children, but only the disease specific CHAQ questionnaire discriminated clearly between the 4 JIA subtypes. In conclusion, the French versions of the CHAQ and the CHQ are reliable, and valid health assessment questionnaires to be used in children suffering from JIA. C1 Hop Necker Enfants Malad, Unite Immunol Hematol & Rheumatol Pediat, F-75745 Paris 15, France. Hop Louis Mourier, Serv Med Interne, F-92701 Colombes, France. IRCCS S Matteo, Lab Informat Med, Pavia, Italy. CHU Brabois, Nancy, France. Hop Purpan, Toulouse, France. Hospices Civils, Lyon, France. Hop Cochin, F-75674 Paris, France. Hop Nord Marseille, Marseille, France. Hop Pellegrin, F-33076 Bordeaux, France. CHU Angers, Angers, France. Hop Jeanne Flandre, Lille, France. CHU Caen, F-14000 Caen, France. Hop Haute Pierre, Strasbourg, France. Hop Mere Enfant, Nantes, France. Hop Sud, Rennes, France. Hop St Jacques, F-25030 Besancon, France. Hop Nord, Grenoble, France. Hop Dupuytren, Limoges, France. Hop Bicetre, INSERM, Paris, France. Ecole Sante Publ, UPRES EA 1124, Fac Med, Vandoeuvre Les Nancy, France. Hop Cochin, Dept Biostat & Med Informat, F-75674 Paris, France. HealthAct, Boston, MA USA. RP Prieur, AM, Hop Necker Enfants Malad, Unite Immunol Hematol & Rheumatol Pediat, 149 Rue Sevres, F-75745 Paris 15, France. TC 11 PD JUL-AUG PY 2001 VL 19 IS 4 SU Suppl. 23 BP S60 EP S65 UT ISI:000170313500013 ER PT J AU Savagner, F Chevrollier, A Loiseau, D Morgan, C Reynier, P Clark, O Stepien, G Malthiery, Y TI Mitochondrial activity in XTC.UC1 cells derived from thyroid oncocytoma SO THYROID ID TRANSCRIPTION FACTOR; OXIDATIVE-PHOSPHORYLATION; SKELETAL-MUSCLE; EXPRESSION; NUCLEAR; GENE; THERMOGENESIS; MYOPATHIES; CARCINOMA; DISEASES AB Thyroid oncocytoma is characterized by the presence of oncocytes containing abnormally large numbers of mitochondria. However, the relationship between the abundance of mitochondria and the pathogenesis of the tumors is unknown. Recently, a new cell line, named XTC.UC1, has been derived from a metastasis of thyroid oncocytoma. We have studied the metabolism and the gene expression profile of the mitochondria in XTC.UC1 cells, using B-CPAP cells as controls. There were no signs of mitochondrial respiratory chain defects or uncoupling between the respiratory chain and adenosine triphosphate (ATP) production. In XTC.UC1 cells, mtDNA transcripts were increased more than fivefold than in controls, in parallel with a 3.6-fold increase in mtDNA content. Finally, in spite of the glycolytic metabolism induced by the culture medium, the mitochondria of XTC.UC1 cells possess the phenotype of oncocytic cells with hypertrophic mitochondria, higher respiratory enzyme activity and higher mtDNA content than in controls. XTC.UC1 cells may therefore offer a useful model for investigating the coordination of the nuclear and mitochondrial genomes, in the context of thyroid tumors. C1 Fac Med Angers, Inserm EMI U 00 18, Lab Biochim & Biol Mol, Angers, France. Univ Calif San Francisco, Mt Zion Med Ctr, Surg Serv, San Francisco, CA 94143 USA. RP Savagner, F, CHU Angers, Lab Biochim & Biol Mol, 4 Rue Larrey, F-49033 Angers, France. TC 11 PD APR PY 2001 VL 11 IS 4 BP 327 EP 333 UT ISI:000168507400004 ER PT J AU Payan, C Cottin, J Lemarie, C Ramont, C TI Inactivation of hepatitis B virus in plasma by hospital in-use chemical disinfectants assessed by a modified HepG2 cell culture SO JOURNAL OF HOSPITAL INFECTION DE Hepatitis B virus; chemical disinfectants; HepG2 cell culture ID VIRUCIDAL ACTIVITY; MODEL; REPLICATION; INFECTIVITY; PCR AB Because of the difficulties of the chimpanzee model and the genetic differences using the duck model, we developed a cell culture method to measure human hepatitis B virus (HBV) inactivation in vitro. Pooled HBV-infected human plasma that had been exposed to a disinfectant was left in contact for three days with a cell culture of the human hepatoma cell line, HepG2, with 4% polyethyleneglycol and 3 mM sodium butyrate. The mean log(10) of the viral titre of unexposed plasma was 4.87 infectious units per mL. Our results showed that 1% glutaraldehyde, sodium hypochlorite at 4700 ppm free chlorine and an iodophor-detergent disinfectant containing 3.6% povidone-iodine reduced viral titres by factors exceeding 10(3)-10(4). However, sodium hypochlorite at 1000 ppm free chlorine had minimal activity and povidone-iodine at 9, 5 and 3.6% had no measurable activity (less than 10-fold reduction). This is the first study using a cell culture model to assess disinfectant activity against HBV. It demonstrates more rapidly than the chimpanzee model that glutaraldehyde and sodium hypochlorite, using standard concentrations and exposure times compatible with clinical practice, were highly active against HBV. However, unexpectedly for an enveloped virus, we found no antiviral activity for iodine in the absence of detergent. (C) 2001 The Hospital Infection Society. C1 CHU Angers, Lab Bacteriol Virol Hyg Hosp, F-49033 Angers, France. RP Payan, C, CHU Angers, Lab Bacteriol Virol Hyg Hosp, 4 Rue Larrey, F-49033 Angers, France. TC 11 PD APR PY 2001 VL 47 IS 4 BP 282 EP 287 UT ISI:000168495500006 ER PT J AU Hambli, R TI Finite element model fracture prediction during sheet-metal blanking processes SO ENGINEERING FRACTURE MECHANICS DE fracture; damage; blanking process; finite element; experiment ID GROWTH AB In order to accurately simulate sheet-metal cutting processes by shearing mechanisms, such as blanking and punching, we have developed a finite element model (FEM) valid for the numerical description of such processes. Damage and crack propagation have been taken into account by means of an elastoplastic constitutive law. To study the effects of varying the process parameters on the geometry of the sheared edges, and the evolution of the force-punch penetration, we have implemented a calculation algorithm by means of the user routine (UMAT) of ABAQUS/standard finite element code. Final results given by the FEM were compared with the experimental ones. (C) 2001 Elsevier Science Ltd. All rights reserved. C1 ISTIA, LASQUO, F-49000 Angers, France. ENSAM, LPMI, F-49035 Angers, France. RP Hambli, R, ISTIA, LASQUO, 62 Ave Notre Dame du Lac, F-49000 Angers, France. TC 11 PD FEB PY 2001 VL 68 IS 3 BP 365 EP 378 UT ISI:000166953400007 ER PT J AU Lapied, B Grolleau, F Sattelle, DB TI Indoxacarb, an oxadiazine insecticide, blocks insect neuronal sodium channels SO BRITISH JOURNAL OF PHARMACOLOGY DE insect; DUM neurones; oxadiazine; insecticide; local anaesthetic; neuronal sodium channels ID ADULT AMINERGIC NEURONS; CELLS DUM NEURONS; PYRETHROID INSECTICIDES; PACEMAKER ACTIVITY; ION CHANNELS; PATCH-CLAMP; COCKROACH; CURRENTS; IDENTIFICATION; LIDOCAINE AB 1 Decarbomethoxyllated JW062 (DCJW), the active component of a new oxadiazine insecticide DPX-JW062 (Indoxacarb). was tested on action potentials and the inward sodium current recorded from short-term cultured dorsal unpaired median neurones of the cockroach Periplaneta americana. 2 Under whole-cell current-clamp conditions, 100 nM DCJW reduced the amplitude of action potentials and induced a large hyperpolarization of the resting membrane potential associated with a 41% increase in input resistance. 3 In voltage-clamp, DCJW resulted in a dose-dependent inhibition (IC50 28 nM) of the peak sodium current. Based on IC50 values, the effect of DCJW was about 10 fold less potent than tetrodotoxin (TTX) but 1000 fold more potent than the local anaesthetic lidocaine. DCJW (100 nM) was without effect on activation properties of the sodium current, reversal potential, voltage dependence of sodium conductance and on both fast and slow steady-state inactivations. 4 TTX (2 nM) resulted in 48% inhibition of the peak inward sodium current. Go-application of TTX (2 nM) with various concentrations of DCJW produced an additional inhibition of the peak inward current, indicating that the blocking actions of DCJW and TTX were distinct. Coapplication of lidocaine (IC50 30 muM) with various concentrations of DCJW produced a reduction of the apparent potency of DCJW, suggesting that DCJW and lidocaine acted at the same site. 5 DCJW (100 nM) did not affect inward calcium or outward potassium currents. 6 This study describes, for the first time, the action on insect neuronal voltage-dependent sodium channels of Indoxacarb, a new class of insecticides. C1 Univ Angers, UFR Sci, UPRES EA 2647, RCIM,Lab Neurophysiol, F-49045 Angers, France. Univ Oxford, Dept Human Anat & Genet, MRC, Funct Genet Unit, Oxford OX1 3QX, England. RP Lapied, B, Univ Angers, UFR Sci, UPRES EA 2647, RCIM,Lab Neurophysiol, 2 Bd Lavoisier, F-49045 Angers, France. TC 11 PD JAN PY 2001 VL 132 IS 2 BP 587 EP 595 UT ISI:000166736500025 ER PT S AU Bancewicz, T Le Duff, Y Godet, JL TI Multipolar polarizabilities from interaction-induced Raman scattering SO MODERN NONLINEAR OPTICS, PT 1, SECOND ED SE ADVANCES IN CHEMICAL PHYSICS ID INDUCED LIGHT-SCATTERING; BODY PERTURBATION-THEORY; LINE-SHAPE; TETRAHEDRAL MOLECULES; GASEOUS NITROGEN; INDUCED SPECTRA; MOMENT ANALYSIS; CF4; CH4; GAS C1 Adam Mickiewicz Univ Poznan, Fac Phys, Div Nonlinear Opt, Poznan, Poland. Univ Angers, Fac Sci, Lab Proprietes Opt Mat & Applicat, Angers, France. RP Bancewicz, T, Adam Mickiewicz Univ Poznan, Fac Phys, Div Nonlinear Opt, Poznan, Poland. TC 10 PY 2001 VL 119 PN Part 1 Sp. Iss. 2 BP 267 EP 307 UT ISI:000177164700006 ER PT J AU Hambli, R TI Comparison between Lemaitre and Gurson damage models in crack growth simulation during blanking process SO INTERNATIONAL JOURNAL OF MECHANICAL SCIENCES DE blanking; FEM; crack growth simulation; damage modelling; experiment ID ELEMENT AB In this paper, the numerical results obtained by a finite element analysis in the case of sheet metal blanking process are compared with the experimental ones to verify the validity of Gurson and Lemaitre damage models in describing the initiation and propagation of cracks during the process evolution. The concept of continuum damage mechanics has been retained to describe the progressive damage accumulation into the sheet metal leading to the final rupture. During the analysis, the crack propagation is simulated by the propagation of a completely damaged area. The comparative study of the results obtained by simulations using different damage models and experimental ones, showed that Gurson damage model is not able to predict the fracture propagation path in a realistic way. Only Lemaitre damage model gives good results. (C) 2001 Elsevier Science Ltd. All rights reserved. C1 ISTIA, LASQUO, Upres JE 2039, F-49000 Angers, France. RP Hambli, R, ISTIA, LASQUO, Upres JE 2039, 62 Ave Notre Dame du Lac, F-49000 Angers, France. TC 10 PD DEC PY 2001 VL 43 IS 12 BP 2769 EP 2790 UT ISI:000172633800004 ER PT J AU Bretones, P Duprez, L Parma, J David, M Vassart, G Rodien, P TI A familial case of congenital hypothyroidism caused by a homozygous mutation of the thyrotropin receptor gene SO THYROID ID TRANSCRIPTION FACTOR-I; THYROID DYSGENESIS; INACTIVATING MUTATION; RESPIRATORY-FAILURE; NADPH OXIDASE; CLEFT-PALATE; TSH; THYROGLOBULIN; HYPOPLASIA; DELETION AB Most of the time congenital hypothyroidism appears as a sporadic disease. In addition to the rare defects in hormonosynthesis associated with goiters, the causes of congenital hypothyroidism include agenesis and ectopy of the thyroid gland. The study of some familial cases has allowed the identification of a few genes responsible for congenital hypothyroidism. We report here a familial case of congenital hypothyroidism, transmitted as a recessive trait, and caused by a homozygous mutation in the thyrotropin receptor (TSH-R). The initial diagnosis of thyroid agenesis, based on the absence of tracer uptake on scintiscan, was incorrect, because ultrasound examination identified severely hypoplastic thyroid tissue in the cervical region. C1 IRIBHN, B-1070 Brussels, Belgium. Hop Lyon Sud, Dept Pediatry, Lyon, France. Free Univ Brussels, Dept Med Genet, B-1050 Brussels, Belgium. Ctr Hosp Univ, Dept Endocrinol, Angers, France. RP Vassart, G, IRIBHN, Campus Hop Erasme,Route de Lennik 808, B-1070 Brussels, Belgium. TC 10 PD OCT PY 2001 VL 11 IS 10 BP 977 EP 980 UT ISI:000171839000013 ER PT J AU Kim, WS Jock, S Paulin, JP Rhim, SL Geider, K TI Molecular detection and differentiation of Erwinia pyrifoliae and host range analysis of the Asian pear pathogen SO PLANT DISEASE DE Asian pear blight; disease rating ID AMYLOVORA; IDENTIFICATION; TREES; DISEASE; GROWTH; DNA AB The recently described pathogen Erwinia pyrifoliae, isolated from Nashi pear fruit trees in Korea, resembles the fire blight pathogen Erwinia amylovora in some of its properties. The two pathogens were classified into different species by DNA hybridization kinetics and microbiological criteria. From the nucleotide sequences of the 16S rRNA and the internal transcribed spacer (ITS) region as well as extracellular polysaccharide (EPS)-encoding genes, polymerase chain reaction (PCR) primers were designed that specifically detect E. pyrifoliae but not the fire blight pathogen Erwinia amylovora, and these primers were also applied to identify E. pyrifoliae in necrotic plant material. The genomes of several strains were digested with the restriction enzyme SpeI, and the DNA fragments were analyzed by pulsed-field gel electrophoresis (PFGE). Three groups of patterns could be distinguished for the isolated E. pyrifoliae strains, all different from various E. amylovora strains, which produce a relatively homogeneous PFGE pattern after SpeI digests. Typical fire blight host plants were assayed in a growth chamber or an experimental field for their susceptibility to E. pyrifoliae. A strong preference was found for pear varieties, whereas apple, cotoneaster, hawthorn, or raspberry rarely produced necrotic symptoms. E. pyrifoliae was readily detected in samples from pear orchards in South Korea during 1995 to 1998; however, the Asian pear pathogen was not recovered in necrotic plant tissue from 1999 and 2000. C1 Max Planck Inst Cell Biol, D-68526 Ladenburg, Germany. Univ Angers, UMR Pathol Vegetale, INRA, INH, F-49071 Beaucouze, France. Hallym Univ, Dept Gen Engn, Coll Nat Sci, Chunchon 200702, Kangwon Do, South Korea. RP Geider, K, Max Planck Inst Cell Biol, D-68526 Ladenburg, Germany. TC 10 PD NOV PY 2001 VL 85 IS 11 BP 1183 EP 1188 UT ISI:000171699600011 ER PT J AU Lemaire, L Roullin, VG Franconi, F Venier-Julienne, MC Menei, P Jallet, P Le Jeune, JJ Benoit, JP TI Therapeutic efficacy of 5-fluorouracil-loaded microspheres on rat glioma: a magnetic resonance imaging study SO NMR IN BIOMEDICINE DE microspheres; 5-flourouracil; MRI; malignant glioma; drug delivery ID MALIGNANT GLIOMA; BRAIN-TUMORS; DIFFUSION; CHEMOTHERAPY; POLYMERS; 1,3-BIS(2-CHLOROETHYL)-1-NITROSOUREA; RELAXATION; DELIVERY; RELEASE; GROWTH AB The aim of this work was to assess the therapeutic efficacy of an intratumoral bolus injection of 5-fluorouracil (FU) compared to that of drug loaded in biodegradable microspheres, for the treatment of brain tumour. Experiments were carried out using a fast-growing C6-glioma rat model. The therapeutic protocols were performed 12 days after the injection of glioma cells. At this stage, the tumours, were installed and the mean volume was 13 +/- 2 mul as measured by proton magnetic resonance (MR) imaging. This technique was used for the follow-up of the tumour volume with respect to time and therapy. In terms of rat survival, both therapies induced a significant 50% increase in animal life span (p < 0.05) compared to animals receiving no drug or unloaded microspheres. Whilst no cure was observed, analysis of the MR images showed that the local and sustained delivery of FU slowed the tumour development in the vicinity of the microspheres by a factor of 3, compared with the bolus intratumoral injection. Copyright (C) 2001 John Wiley & Sons, Ltd. C1 INSERM, F-49100 Angers, France. Univ Angers, Serv Commun RMN, Angers, France. RP Lemaire, L, INSERM, ERIT M 0104,BAT 1BT,10 Rue Bocquel, F-49100 Angers, France. EM laurent.lemaire@univ-angers.fr TC 10 PD OCT PY 2001 VL 14 IS 6 BP 360 EP 366 UT ISI:000171522000003 ER PT J AU Stephens, DP Charkoudian, N Benevento, JM Johnson, JM Saumet, JL TI The influence of topical capsaicin on the local thermal control of skin blood flow in humans SO AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY DE nociceptors; warmth perception; laser Doppler; local warming ID HUMAN FINGER SKIN; CUTANEOUS ACTIVE VASODILATION; COLD-INDUCED VASOCONSTRICTION; LASER-DOPPLER FLOWMETRY; NITRIC-OXIDE; NEUROGENIC INFLAMMATION; ALPHA-ADRENOCEPTORS; SENSORY NEURONS; TEMPERATURE; NERVES AB To test whether heat-sensitive receptors participate in the cutaneous vascular responses to direct heating, we monitored skin blood flow (SkBF; laser Doppler flowmetry) where the sensation of heat was induced either by local warming (T-Loc; Peltier cooling/heating unit) or by both direct warming and chemical stimulation of heat-sensitive nociceptors (capsaicin). In part I, topical capsaicin (0.075 or 0.025%) was applied to 12 cm(2) of skin 1 h before stepwise local warming of untreated and capsaicin-treated forearm skin. Pretreatment with 0.075% capsaicin cream shifted the SkBF/T-Loc relationship to lower temperatures by an average of 6 +/-0.8 degreesC (P<0.05). In part II, we used a combination of topical capsaicin (0.025%) and local warming to evoke thermal sensation at one site and only local warming to evoke thermal sensation at a separate site. Cutaneous vasomotor responses were compared when the temperatures at these two sites were perceived to be the same. SkBF differed significantly between capsaicin and control sites when compared on the basis of actual temperatures, but that difference became insignificant when compared on the basis of the perceived temperatures. These data suggest heat-sensitive nociceptors are important in the cutaneous vasodilator response to local skin warming. C1 Univ Texas, Hlth Sci Ctr, Dept Physiol, San Antonio, TX 78229 USA. Texas Lutheran Univ, Seguin, TX 78155 USA. CHU Angers, Lab Physiol & Explorat Vasc, F-49045 Angers, France. RP Johnson, JM, Univ Texas, Hlth Sci Ctr, Dept Physiol, 7703 Floyd Curl Dr, San Antonio, TX 78229 USA. TC 10 PD SEP PY 2001 VL 281 IS 3 BP R894 EP R901 UT ISI:000170558400026 ER PT J AU Vasquez, M Hao, JK TI A "logic-constrained" knapsack formulation and a tabu algorithm for the daily photograph scheduling of an earth observation satellite SO COMPUTATIONAL OPTIMIZATION AND APPLICATIONS DE tabu search; heuristics; satellite photograph scheduling; multidimensional knapsack; constrained combinatorial optimization AB The daily photograph scheduling problem of earth observation satellites such as Spot 5 consists of scheduling a subset of mono or stereo photographs from a given set of candidates to different cameras. The scheduling must maximize a profit function while satisfying a large number of constraints. In this paper, we first present a formulation of the problem as a generalized version of the well-known knapsack model, which includes large numbers of binary and ternary "logical" constraints. We then develop a tabu search algorithm which integrates some important features including an efficient neighborhood, a dynamic tabu tenure mechanism, techniques for constraint handling, intensification and diversification. Extensive experiments on a set of large and realistic benchmark instances show the effectiveness of this approach. C1 Ecole Mines Ales, F-30035 Nimes, France. Univ Angers, F-49045 Angers 01, France. RP Vasquez, M, Ecole Mines Ales, Parc Sci G Besse, F-30035 Nimes, France. TC 10 PD NOV PY 2001 VL 20 IS 2 BP 137 EP 157 UT ISI:000170488800002 ER PT J AU Vasquez, M Hao, JK TI A heuristic approach for antenna positioning in cellular networks SO JOURNAL OF HEURISTICS DE large scale combinatorial optimization; tabu search; radio network planning ID CHANNEL ASSIGNMENT; RADIO NETWORKS AB The antenna-positioning problem concerns finding a set of sites for antennas from a set of pre-defined candidate sites, and for each selected site, to determine the number and types of antennas, as well as the associated values for each of the antenna parameters. All these choices must satisfy a set of imperative constraints and optimize a set of objectives. This paper presents a heuristic approach for tackling this complex and highly combinatorial problem. The proposed approach is composed of three phases: a constraint-based pre-processing phase to filter out bad configurations, an optimization phase using tabu search, and a post-optimization phase to improve solutions given by tabu search. To validate the approach, computational results are presented using large and realistic data sets. C1 EERIE, EMA, LGI2P, F-30035 Nimes 01, France. Univ Angers, LERIA, F-49045 Angers, France. RP Vasquez, M, EERIE, EMA, LGI2P, Parc Sci G Besse, F-30035 Nimes 01, France. TC 10 PD SEP PY 2001 VL 7 IS 5 BP 443 EP 472 UT ISI:000170226600002 ER PT J AU Mouneyrac, C Amiard-Triquet, C Amaird, JC Rainbow, PS TI Comparison of metallothionein concentrations and tissue distribution of trace metals in crabs (Pachygrapsus marmoratus) from a metal-rich estuary, in and out of the reproductive season SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY DE crab; Pachygrapsus marmoratus; Gironde estuary (France); metallothionein; cadmium; copper; zinc; reproductive period ID OYSTERS CRASSOSTREA-GIGAS; NATURAL FACTORS SALINITY; CARCINUS-MAENAS L; CALLINECTES-SAPIDUS; BLUE-CRAB; LITTORINA-LITTOREA; FRESH-WATER; MOLT CYCLE; CADMIUM; COPPER AB Crabs, Pachygrapsus marmoratus, were sampled in June 1997 and February 1998 from two sites (at the mouth and 25 km upstream) in the metal-rich Gironde estuary, France. Gills and hepatopancreas were analysed for metal (Cd, Cu, Zn) and metallothionein (MT) contents, in order to examine the influence of both biological and environmental factors on the physico-chemical forms of detoxified metal storage in the crabs. The concentrations of MT and both cytosolic and insoluble metals were not greatly different between males and females, and the influence of organ weights was also minimal. Intersite differences were observed, probably resulting from the gradient of salinity in the estuary, which interacts with both the chemical speciation and bioavailability of metals, and the general protein metabolism of the crabs. Seasonal changes were also important, probably in interaction with the moult and reproductive cycles. In February, concentrations of insoluble metals were generally higher than in June, in both organs, suggesting that essential metals, particularly Zn, are stored during winter then remobilised during the breeding season. The natural variability in the concentrations of MT often concealed any relationship with accumulated metal concentrations. Thus MT in crabs cannot be considered as a useful biomarker of metal pollution. (C) 2001 Elsevier Science Inc. All rights reserved. C1 IRFA, Dept Sci Vie & Terre, Lab Ecol Anim, F-49100 Angers, France. ISOMer, Fac Pharm 1, Serv Ecotoxicol, CNRS,GDR 1117, F-44035 Nantes 1, France. Nat Hist Museum, Dept Zool, London SW7 5BD, England. RP Mouneyrac, C, IRFA, Dept Sci Vie & Terre, Lab Ecol Anim, 44 Rue Rabelais, F-49100 Angers, France. TC 10 PD JUL PY 2001 VL 129 IS 3 BP 193 EP 209 UT ISI:000169953400002 ER PT J AU Ohnuki, H Desbat, B Giffard, M Izumi, M Imakubo, T Mabon, G Delhaes, P TI Formation of metallic Langmuir film on the water surface with fatty acid and BEDO-TTF molecules SO JOURNAL OF PHYSICAL CHEMISTRY B ID FT-IR SPECTROSCOPY; BLODGETT-FILMS; ELECTRICAL-PROPERTIES; THIN-FILMS; REFLECTANCE; INTERFACE; SALTS; TETRACYANOQUINODIMETHANE; TRANSMITTANCE; DERIVATIVES AB The formation process of metallic Langmuir film on the water surface fabricated from BEDO-TTF (BO) and stearic acid (SA) is described. The in-situ observation by Brewster angle microscope revealed a creation of small islands after spreading the mixing solution on the water surface and then a formation of homogeneous Langmuir film by a coalescence of the islands with decreasing of the surface area. An appearance of mixed valence state of BO in both islands and Langmuir film was confirmed by polarization-modulated IR reflection absorption spectroscopy. A theoretical simulation of the spectra supports the bilayer model on the water surface, in which a metallic BO layer underlies between the SA layer and the water surface. An investigation on the Langmuir-Blodgett (LB) films transferred onto substrates by infrared spectroscopy implies the existence of hydrogen-bonded carboxylate groups of (R-COO . . .H . . . OOC-R)(-) in the SA layer. Finally, the optical conductivity in the LB films can be compared with those of the superconductor crystal (BO)(2)ReO4(H2O) through the Drude optical parameters obtained from the IR spectra fitting. C1 Ctr Rech Paul Pascal, F-33600 Pessac, France. RIKEN, Condensed Mol Mat Lab, Wako, Saitama 3510198, Japan. Tokyo Univ Mercantile Marine, Appl Phys Lab, Koto Ku, Tokyo 1358533, Japan. Univ Angers, UMR CNRS 6501, Lab Ingn Mol & Mat Organ, F-49045 Angers, France. Univ Bordeaux 1, UMR 5803 CNRS, Lab Physicochim Mol, F-33405 Talence, France. RP Ohnuki, H, Ctr Rech Paul Pascal, Ave A Schweitzer, F-33600 Pessac, France. TC 10 PD MAY 31 PY 2001 VL 105 IS 21 BP 4921 EP 4927 UT ISI:000169036800020 ER PT J AU Sylvestre, F Servant-Vildary, S Roux, M TI Diatom-based ionic concentration and salinity models from the south Bolivian Altiplano (15-23 degrees S) SO JOURNAL OF PALEOLIMNOLOGY DE Bolivia; modern diatom flora; salinity; ionic composition; transfer function ID NORTH-AMERICA; LAKES; ASSEMBLAGES; RECONSTRUCTION; FLUCTUATIONS; HOLOCENE; TOOL; PH AB The relationship between surface sediment diatom assemblages and measured limnological variables in fifty eight lake samples from the south Bolivian Altiplano was examined by constructing a diatom-water chemistry dataset. Analysis of this dataset by canonical correspondence analysis revealed that salinity and ionic concentration accounted for a significant amount of the variation in the distribution of the diatom assemblages. Two methods 'weighted-averaging regression and calibration', and 'by-class mean percentage table' were used to established a transfer function for future reconstruction of past lake water salinity and ionic concentration in the southern Bolivian Altiplano. Weighted-averaging regression and calibration with inverse deshrinking provided a better model for the water chemistry reconstructions in this region. C1 Univ Angers, Geol Lab, EA 2644, F-49045 Angers, France. Museum Natl Hist Nat, Antenne IRD Orstom, Geol Lab, F-75005 Paris, France. Univ Aix Marseille 3, Lab Biomath, F-13397 Marseille, France. RP Sylvestre, F, Univ Angers, Geol Lab, EA 2644, 2 Blvd Lavoisier, F-49045 Angers, France. TC 10 PD APR PY 2001 VL 25 IS 3 BP 279 EP 295 UT ISI:000167852600002 ER PT J AU Millot, F Suciu, S Philippe, N Benoit, Y Mazingue, F Uyttebroeck, A Lutz, P Mechinaud, F Robert, A Boutard, P Marguerite, G Ferster, A Plouvier, E Rialland, X Behard, C Plantaz, D Dresse, MF Philippet, P Norton, L Thyss, A Dastugue, N Waterkeyn, C Vilmer, E Otten, J CA Childrens Leukemia Cooperative G TI Value of high-dose cytarabine during interval therapy of a Berlin-Frankfurt-Munster-based protocol in increased risk children with acute lymphoblastic leukemia and lymphoblastic lymphoma: Results of the European Organization for Research and Treatment of Cancer 58881 Randomized Phase III Trial SO JOURNAL OF CLINICAL ONCOLOGY ID PEDIATRIC-ONCOLOGY-GROUP; ACUTE LYMPHOCYTIC-LEUKEMIA; PARAFFIN-EMBEDDED TISSUE; NON-HODGKINS LYMPHOMA; CYTOSINE-ARABINOSIDE; INTRATHECAL METHOTREXATE; CRANIAL RADIOTHERAPY; CEREBROSPINAL-FLUID; MENINGEAL LEUKEMIA; CNS RELAPSE AB Purpose: The European Organization for Research and Treatment of Cancer 58881 study was designed to test in a prospective multicentric randomized trial the value of high-dose (HD) intravenous (IV) cytarabine (Ara-C) added to HD IV methotrexate (MTX) to reduce the incidence of CNS and systemic relapses in children with increased-risk acute lymphoblastic leukemia (ALL) or stage III and IV lymphoblastic lymphoma treated with a Berlin-Frankfurt-Munster (BFM)-based regimen. Patients and Methods: After completion of induction-consolidation phase, children with increased-risk (risk factor > 0.8 or T-lineage) ALL or stage III and IV lymphoblastic lymphoma were randomized to receive four courses of I-ID MTX (5 g/m(2) over 24 hours every 2 weeks) and four intrathecal administrations of MTX (Arm A) or the same treatment schedule with additional I-ID IV Ara-C (1 g/m(2) in bolus injection 12 and 24 hours after the start of: each MTX infusion) (Arm B). Results: Between January 1990 and January 1996, 653 patients with ALL (593 patients) or lymphoblastic lymphoma (60 patients) were randomized: 323 were assigned to Arm A (without Ara-C) and 330 to Arm B (with Ara-C). A total of 190 events (177 relapses and 13 deaths without relapse) were reported, and the median follow up was 6.5 years (range, 2 to 10 years). The incidence rates of CNS relapse were similar in both arms whether isolated (5.6% and 3.3%, respectively) or combined (5.3% and 4.6%, respectively). The estimated 6-year disease-free survival (DFS) rate was similar (log-rank P = .67) in the two treatment groups: 70.4% (SE = 2.6%) in Arm A and 71.0% (SE = 2.5%) in Arm B. The 6-year DFS rate was similar for ALL and LL patients: 70.2% (SE = 1.9%) versus 76.3% (SE = 5.6%). Conclusion: Prevention of CNS relapse was satisfactorily achieved with HD IV MTX and intrathecal injections of MTX in children with increased-risk ALL or stage III and IV lymphoblastic lymphoma treated with our BFM-based treatment protocol in which cranial irradiation was omitted. Disappointingly, with the dose schedule used in this protocol, HD Ara-C added to HD MTX, although well tolerated, failed to further decrease the incidence of CNS relapse or to improve the overall DFS. (C) 2001 by American Society of Clinical Oncology. C1 Univ Hosp Poitiers, Dept Pediat, Poitiers, France. Univ Hosp Lyon, Dept Pediat, Lyon, France. Ctr Hosp Reg & Univ Lille, Dept Pediat, F-59037 Lille, France. Univ Hosp Strasbourg, Dept Pediat, Strasbourg, France. Univ Hosp Nantes, Dept Pediat, Nantes, France. Univ Paris, Hosp R Debre, Dept Pediat, F-75252 Paris, France. Univ Hosp Toulouse, Dept Pediat, Toulouse, France. Univ Hosp Caen, Dept Pediat, Caen, France. Univ Hosp Montpellier, Dept Pediat, Montpellier, France. Univ Hosp Besancon, Dept Pediat, Besancon, France. Univ Hosp Angers, Dept Pediat, Angers, France. Univ Hosp Reims, Dept Pediat, Reims, France. Univ Hosp Reims, Dept Pediat, Grenoble, France. Univ Hosp Verviers, Dept Pediat, Verviers, Belgium. State Univ Ghent Hosp, Dept Pediat, B-9000 Ghent, Belgium. Free Univ Brussels, Hosp Reine Fabiola, Akad Ziekenhuis, B-1090 Brussels, Belgium. Univ Hosp Porto, Dept Pediat, Porto, Portugal. Univ Hosp Toulouse, Lab Hematol Cytol, Toulouse, France. European Org Res Treatment Canc, Ctr Data, Brussels, Belgium. RP Millot, F, Univ Hosp Poitiers, Dept Hematol & Med Oncol, 350 Ave Jacques Coeur,BP 557, F-86021 Poitiers, France. TC 10 PD APR 1 PY 2000 VL 19 IS 7 BP 1935 EP 1942 UT ISI:000167920300007 ER PT J AU Andre, T Colin, P Louvet, C Gamelin, E Bouche, O Achille, E Colbert, N Boaziz, C Piedbois, P Tubiana-Mathieu, N Boutan-Laroze, A Flesch, M Billiau, V Buyse, M de Gramont, A CA Grp Etud Rech Clin Oncologie Rech TI Randomized adjuvant study comparing two schemes of 5-fluorouracil and leucovorin in stage B2 and C colon adenocarcinoma: Study design and preliminary safety results SO SEMINARS IN ONCOLOGY ID ADVANCED COLORECTAL-CANCER; HIGH-DOSE LEUCOVORIN; INFUSION FLUOROURACIL; PHASE-III; CARCINOMA; TRIAL; MODULATION; BOLUS C1 Hop Tenon, Med Oncol Serv, F-75970 Paris 20, France. Hop St Antoine, F-75571 Paris, France. Ctr Paul Papin, Angers, France. CHU Reims, Reims, France. Clin Orangerie, Strasbourg, France. Hop St Joseph, F-75674 Paris, France. Ctr Paris Nord, Sarcelles, France. Hop Henri Mondor, F-94010 Creteil, France. CHU Limoges, Limoges, France. Ctr Hosp Argenteuil, Argenteuil, France. Hop Devron, Dijon, France. Int Inst Drug Dev, Brussels, Belgium. Int Inst Drug Dev, Paris, France. Grp Etud & Rech Clin Oncol Radiotherapy, Paris, France. RP Andre, T, Hop Tenon, Med Oncol Serv, 4 Rue Chine, F-75970 Paris 20, France. TC 10 PD FEB PY 2001 VL 28 IS 1 SU Suppl. 1 BP 35 EP 40 UT ISI:000167043100008 ER PT J AU Poupard, P Brunel, N Leduc, N Viemont, JD Strullu, DG Simoneau, P TI Expression of a Bet v 1 homologue gene encoding a PR 10 protein in birch roots: induction by auxin and localization of the transcripts by in situ hybridization SO AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY ID PATHOGENESIS-RELATED PROTEINS; BET-V-1; FAMILY; STRESS; CYTOKININ; ALLERGEN; PATTERNS; MEMBERS; PENDULA AB In birch roots (Betula pendula Roth), two members of the Bet v 1 gene family which encode PR 10 proteins have previously been characterized. One of these members, named Bet v 1-sc1, is significantly induced in response to biotic or abiotic factors. We have analysed the expression of Bet v 1-sc1 in birch roots treated either with 1 muM indole-3-acetic acid (IAA) or 1 muM kinetin using reverse transcription-polymerase chain reaction (RT-PCR), northern blotting and competitive PCR. High accumulation of the Bet v 1-sc1 transcripts was recorded only after auxin application, while kinetin had no effect. By in situ hybridization, we have investigated the localization of Bet v 1-sc1 mRNA in birch roots after induction of the gene by root treatment with 1 muM IAA. Using root tip sections, we showed that Bet v 1-sc1 is significantly expressed in the apical meristem and the procambium. In sections taken in the zone producing lateral roots, the presence of Bet v 1-sc1 was found at sites of emerging secondary root primordia. This first report of localization of Bet v 1-sc1 expression suggests that this gene could be involved in the processes leading to lateral root initiation. C1 Univ Angers, Fac Sci, Lab Biol & Physiol Vegetale, F-49045 Angers, France. RP Poupard, P, Univ Angers, Fac Sci, Lab Biol & Physiol Vegetale, 2 Bd Lavoisier, F-49045 Angers, France. TC 10 PY 2001 VL 28 IS 1 BP 57 EP 63 UT ISI:000166730200006 ER PT J AU Cartier, C Faure, M Lardeux, H TI The Hercynian orogeny in the South Armorican Massif (Saint-Georges-sur-Loire Unit, Ligerian Domain, France): rifting and welding of continental stripes SO TERRA NOVA ID BELT; EVOLUTION; ECLOGITES; BRITTANY AB A new approach to explain the Western Hercynian Belt is given by a lithological and structural study of the St-Georges-sur-Loire Unit (southern part of the French Armorican Massif). This unit is interpreted as a Devonian olistostrome sheared to the North. It is formed in an accretionary prism linked with a south-dipping subduction zone, called the Layon subduction zone. It results from a chaotic sedimentation including exotic blocks of various lithologies and ages in a Middle Devonian pebbly mudstone matrix. An inovative geodynamic model involving microblock rifting and collision is discussed to explain the presence of the St-Georges-sur-Loire Unit in the Armorican Massif. This model involves two Palaeozoic subductions and emphasizes the role of the Nort-sur-Erdre fault, considered as a major polyphase suture zone separating South and Central Armorica. C1 ISTO, CNRS, UMR 6213, F-45067 Orleans 2, France. Univ Catholique Ouest, IRFA, F-49008 Angers, France. RP Cartier, C, ISTO, CNRS, UMR 6213, Batiment Geosci,BP 6759, F-45067 Orleans 2, France. TC 9 PD APR PY 2001 VL 13 IS 2 BP 143 EP 149 UT ISI:000173047300012 ER PT J AU Skarka, V Aleksic, NB Berezhiani, VI TI Dynamics of electromagnetic beam with phase dislocation in saturable nonlinear media SO PHYSICS LETTERS A DE soliton; vortex; Gaussian beam; nonlinear Schrodinger ID 3-DIMENSIONAL OPTICAL SOLITONS; SELF-FOCUSING MEDIUM; TRAPPED BEAMS; GENERATION; VORTEX; LOGIC; KERR AB The nonlinear dynamics of laser beams carrying phase singularity in media with cubic-quintic nonlinearity is studied. In such media can be generated not only localized vortex solitons but also a novel kind of stable nonlocalized optical vortices. Stability in the defocusing regime is confirmed numerically. In the focusing regime such a vortex breaks into filaments. Dynamics of a singular Gaussian beam is investigated. Numerical simulations show a new behavior of the supercritical Gaussian beam which first breaks into filaments coalescing after. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, UMR 6136 CNRS, Lab POMA, F-49045 Angers 1, France. Inst Phys, YU-11000 Belgrade, Yugoslavia. Free Univ Brussels, B-1050 Brussels, Belgium. RP Skarka, V, Univ Angers, UMR 6136 CNRS, Lab POMA, 2 Blvd Levoisier, F-49045 Angers 1, France. TC 9 PD DEC 3 PY 2001 VL 291 IS 2-3 BP 124 EP 132 UT ISI:000172707300011 ER PT J AU Lode, T TI Mating system and genetic variance in a polygynous mustelid, the European polecat SO GENES & GENETIC SYSTEMS ID WESTERN FRANCE; MELES-MELES; POPULATION-STRUCTURE; VULPES-VULPES; CANIS-LUPUS; PUTORIUS L; RED FOX; MAMMALS; ERMINEA; VARIABILITY AB The population genetic implications of mating system were investigated in European polecat Mustela putorius populations from western France, combining radiotracking survey and allozyme variation analysis. Mating period occurred from February to June and polecats showed a strategy of successive polygyny, a male consorting with 1.44 females during a brief period (2.9 days). Relatedness was largely sex biased, females (21%) being almost twice more related than males (13%) suggesting a natal philopatry. Nonetheless, breeding dispersal pattern appeared relatively complex. Males were the sex dispersing but the main strategy for male polecats consisted of short-term mating excursions in adjacent females ranges whereas long-distance dispersal only constituted an alternative breeding strategy. Despite their allozymic polymorphism level reaching 24% at p <0.05 for 38 scored loci, populations showed a high heterozygote deficiency as revealed by the F-IS index averaging F-IS = 0.383. Thus the mating system of such solitary mustelids may be poorly efficient to prevent inbreeding within populations. C1 Univ Angers Belle Beille, Fac Sci, Lab Ecol Anim, F-49045 Angers, France. RP Lode, T, Univ Angers Belle Beille, Fac Sci, Lab Ecol Anim, F-49045 Angers, France. TC 9 PD AUG PY 2001 VL 76 IS 4 BP 221 EP 227 UT ISI:000171979300002 ER PT J AU Misset, JL Vennin, P Chollet, P Pouillart, P Laplaige, P Frobert, JL Castera, D Fabro, M Langlois, D Cortesi, E Lucas, V Gamelin, E Laadem, A Otero, J TI Multicenter phase II-III study of oxaliplatin plus cyclophosphamide vs. cisplatin plus cyclophosphamide in chemonaive advanced ovarian cancer patients SO ANNALS OF ONCOLOGY DE DACH-platinum; neurotoxicity ID DNA MISMATCH REPAIR; STAGE-III; PACLITAXEL; CELLS; EXPRESSION; TRIAL AB Purpose: A phase II-III randomised study to compare safety and efficacy of an oxaliplatin/cyclophosphamide (OXAC) combination, vs. the reference combination of cisplatin/cyclophosphamide (CPC), in untreated advanced ovarian cancer patients. Patients and methods: 182 patients were enrolled, of whom 177 were treated; 86 with OXAC (130 mg/m(2) oxaliplatin two-hour intravenous (i.v.) infusion, 1000 mg/m(2) cyclophosphamide two-hour i.v. infusion), and 91 with CPC (100 mg/m(2) cisplatin one-hour i.v. infusion, 1000 mg/m(2) cyclophosphamide two-hour i.v. infusion). Treatment cycles were repeated every three weeks (maximum of six cycles). Results: The main toxicities, which were significantly less severe in the OXAC arm, were myelosuppression and vomiting, including (OXAC vs CPC, % patients): grade 3-4 leukopenia (37% vs. 56%), and anaemia (7% vs. 32%), with blood transfusions in 8% vs. 21%. In the OXAC arm, 64% of surgically assessable patients and 33% of clinically assessable patients achieved an objective response. In the CPC arm, 67% patients achieved a surgical response and 42% achieved an objective clinical response. In the OXAC and CPC arms, median progression free-survival was 13.0 and 13.3 months, and overall survival was 36.0 and 25.1 months respectively, without statistically significant difference. Conclusion: The activity and time-related parameters of the OXAC and CPC combinations in advanced ovarian cancer patients, are comparable. Combined with the better safety profile of the oxaliplatin-containing regimen, this confirms the interest of oxaliplatin combined with active new agents in this indication. C1 Hop Paul Brousse, Federat Serv Malad Sanguines Immunitaires & Tumor, F-94804 Villejuif, France. Ctr Oscar Lambret, F-59020 Lille, France. Ctr Jean Perrin, Clermont Ferrand, France. Inst Curie, Paris, France. Clin St Come & St Damien, Blois, France. Ctr Hosp, Bourg En Bresse, France. Clin St Pierre, Perpignan, France. Ctr Val Aurelle, Montpellier, France. Ctr St Michel, La Rochelle, France. Policlin Umberto 1, Rome, Italy. Hop Source Orleans, Orleans, France. Ctr Paul Papin, Angers, France. CAC, Le Kremlin Bicetre, France. Inst Nacl Enfermedades Neoplas, Lima, Peru. RP Misset, JL, Hop Paul Brousse, Federat Serv Malad Sanguines Immunitaires & Tumor, 14 Ave Paul Vaillant Couturier,BP 200, F-94804 Villejuif, France. TC 9 PD OCT PY 2001 VL 12 IS 10 BP 1411 EP 1415 UT ISI:000171940400021 ER PT J AU Fournier, HD Mercier, P Menei, P TI Lateral interscalenic multilevel oblique corpectomies to repair ventral root avulsions after brachial plexus injury in humans: anatomical study and first clinical experience SO JOURNAL OF NEUROSURGERY DE avulsion injury; brachial plexus; neural implantation; nerve regeneration ID CERVICAL SPONDYLOTIC MYELOPATHY; NEURITE GROWTH; SPINAL-CORD; REGENERATION; TUMORS; CNS AB Object. Because central nervous system white matter exerts a powerful inhibitory effect on axonal growth, implantation of nerve grafts or rootlets into the cervical spinal cord following ventral root avulsion injury should, ideally, be performed directly through the ventral root exit zone (VRExZ), which is located near the anteromedial aspect of the anterior horn; the grafts/rootlets should not be implanted into the white matter of the lateral cord. This is not possible when using a conservative posterior approach. Therefore, the authors have studied the anatomy encountered when using the anterolateral approach and evaluated the technique in the particular case of avulsed ventral nerve roots. They also present a case illustration of the procedure, which is used currently in their department. Methods. Anterior access to the rootlets is obtained using a lateral interscalenic approach; the vertebral artery is exposed and mobilized, and oblique drilling of the vertebral bodies (VBs) is performed. Because the articular processes and half of the VBs are preserved, fusion is not required. The approach allows the surgeon to expose the anterior aspect of the cervical dura and the entire length of the emerging spinal nerves. The anterior aspect of the dura is opened at the desired levels for VRExZ exposure, and the position is ideal for implantation of the graft/rootlets. The interscalenic dissection is mandatory so that the lesions of the supraclavicular plexus can be evaluated and repaired. If necessary, the anterior approach allows for exploration of the infraclavicular plexus during the same procedure. Conclusions. The use of a true anterior approach to the ventral rootlets appears to be a valuable and appropriate approach that avoids extensive laminectomy/facetectomy while reimplantation is performed through the anterolateral sulcus itself. In this approach, however, reimplantation of dorsal roots into the spinal cord remains impossible. C1 CHU, Serv Neurochirurg, F-49033 Angers 01, France. Fac Med, Lab Anat, Angers, France. RP Fournier, HD, CHU, Serv Neurochirurg, 4 Rue Larrey, F-49033 Angers 01, France. TC 9 PD OCT PY 2001 VL 95 IS 2 SU Suppl. S BP 202 EP 207 UT ISI:000171434600006 ER PT J AU de Bray, JM Pasco, A Tranquart, F Papon, X Alecu, C Giraudeau, B Dubas, F Emile, J TI Accuracy of color-Doppler in the quantification of proximal vertebral artery stenoses SO CEREBROVASCULAR DISEASES DE vertebral arteries; Doppler; sonography; angiography; stenosis ID OCCLUSIVE DISEASE; DUPLEX SONOGRAPHY; ANGIOGRAPHY; ULTRASOUND; ORIGIN AB Background: Vertebrobasilar (VB) strokes appear to have the same causes as carotid strokes. Obstructive lesions of proximal vertebral arteries probably occur in about 30% of stroke patients. Purpose: Our aim was to assess the validity of color Doppler sonography compared to selective! intra-arterial angiography in the quantification of proximal vertebral artery stenoses. Materials and Methods: A prospective blind study of 316 vertebral arteries was undertaken between 1996 and 1998. One hundred and fifty-eight patients with cerebrovascular disorders without cerebral hemorrhage were studied consecutively by frequency or amplitude color Doppler flow imaging and intra-arterial angiography. The lesions were quantified by morphological and hemodynamic criteria and classified into 6 groups: 0% 207 arteries; 1-29% 32 arteries; 30-49% 29 arteries; 50-69% 13 arteries; 70-99% 23 arteries; 100% 12 arteries. Results: Ten of the 12 occlusions were identified, the 2 false-negatives were due to 2 revascularized vessels. Moderate stenoses (< 50%) were differentiated from tight stenoses (> 50%) using hemodynamic criteria. The majority of false-negative stenoses (38) in the different groups were related to intrathoracic or very deep origin of the artery, anechogenic stenosis or a tortuous vessel. Stenoses greater than 70% were diagnosed in 71% of cases with a specificity of 99%. The kappa value was 0.80. Conclusion: Duplex sonography should be proposed first in VB attacks or stroke to detect and quantify vertebral artery stenoses for surgery and angioplasty. Copyright (C) 2001 S. Karger AG, Basel. C1 CHU Angers, Dept Neurol, F-49033 Angers, France. CHU Angers, Dept Radiol, F-49033 Angers, France. CHU Angers, Dept Cardiovasc Surg, F-49033 Angers, France. CHU Tours, Dept Ultrasound, Tours, France. CHU Tours, Dept Clin Res, Tours, France. RP de Bray, JM, CHU Angers, Dept Neurol, F-49033 Angers, France. TC 9 PY 2001 VL 11 IS 4 BP 335 EP 340 UT ISI:000169069200009 ER PT J AU Brillu, C Picquet, J Villapadierna, F Papon, X L'Hoste, P Jousset, Y Enon, B TI Percutaneous transluminal angioplasty for management of critical ischemia in arteries below the knee SO ANNALS OF VASCULAR SURGERY ID TRANS-LUMINAL ANGIOPLASTY; LIMB-THREATENING ISCHEMIA AB Percutaneous transluminal angioplasty (PTA) can be performed safely in arteries below the knee by using current coaxial catheters. This study includes 37 consecutive patients treated between March 1992 and March 1999 by PTA for limb-threatening infrageniculate occlusive artery disease. The mean duration of follow-up was 28 months. Limb salvage was achieved in 32 patients. The actuarial limb salvage rate at 2 years was 87 +/- 6%. This study shows that PTA was a viable alternative to surgical treatment for management of critical lower extremity ischemia in carefully selected patients. Limb salvage rates after PTA and conventional surgical revascularization seem comparable. Based on these findings, we recommend that PTA be attempted, whenever possible, for initial treatment of patients presenting critical, limb-threatening ischemia due to isolated or multiple stenoses of below-knee arteries. C1 Univ Angers, Ctr Hosp, Dept Cardiovasc Thorac Surg, Angers, France. RP Brillu, C, CHU Angers, Serv Chirurg Cardiovasc & Thorac, F-49033 Angers 01, France. TC 9 PD MAR PY 2001 VL 15 IS 2 BP 175 EP 181 UT ISI:000167840900008 ER PT J AU Morgan, CJ Jacques, C Savagner, F Tourmen, Y Mirebeau, DP Malthiery, Y Reynier, P TI A conserved N-terminal sequence targets human DAP3 to mitochondria SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS DE DAP3; mitochondria; targeting; apoptosis ID INDUCED CELL-DEATH; RAT-LIVER; PROTEINS; RECEPTOR AB Human DAP3 (death-associated protein-3) has been identified as an essential positive mediator of programmed cell death. Structure-function studies have shown previously the N-terminal extremity of the protein to be required in apoptosis induction. Analysis of human DAP3 gene structure predicted 13 exons and subsequent targeting prediction by two software packages (MITOPROT and TargetP) gave a high probability for mitochondrial targeting. The predicted N-terminal targeting structure was also found in the mouse, Drosophila, and C. elegans orthologues with a strong sequence homology between mouse and human. Secondary structure analyses identified ar-helical structures typical of mitochondrial target peptides. To confirm experimentally this targeting we constructed a fusion protein with N-terminal human DAP3 upstream of enhanced green fluorescent protein (EG;FP). Confocal analysis of transfected human fibroblasts clearly demonstrated EGFP localization exclusive to mitochondria. The positioning of this key apoptotic factor at the heart of the mitochondrial pathway provides exciting insight into its role in programmed cell death. (C) 2001 Academic Press. C1 CHU Angers, Lab Biochim & Biol Mol, INSERM, EMI U 00 18, F-49033 Angers, France. RP Morgan, CJ, CHU Angers, Lab Biochim & Biol Mol, INSERM, EMI U 00 18, 4 Rue Larrey, F-49033 Angers, France. TC 9 PD JAN 12 PY 2001 VL 280 IS 1 BP 177 EP 181 UT ISI:000166404700030 ER PT J AU Goubert, E Neraudeau, D Rouchy, JM Lacour, D TI Foraminiferal record of environmental changes: Messinian of the Los Yesos area (Sorbas Basin, SE Spain) SO PALAEOGEOGRAPHY PALAEOCLIMATOLOGY PALAEOECOLOGY DE benthic foraminifers; ecological groups; paleoenvironments; biodiversity; 'Messinian Salinity Crisis'; western Mediterranean ID EVAPORITIC LATE MIOCENE; BENTHIC FORAMINIFERA; SALINITY CRISIS; LORCA BASIN; BIOFACIES; SEDIMENTATION; RESTRICTION; SUCCESSIONS; CHRONOLOGY; SAPROPELS AB The Late Messinian of the Sorbas Basin (Belie Cordillera, SE Spain) has been sampled to estimate (1) the paleoenvironmental changes recorded by the benthic foraminifers in pre-evaporitic and evaporitic units. and (2) the impact of the 'Messinian Salinity Crisis' on the foraminiferal biodiversity. In the pre-evaporitic unit, foraminifers indicate a circalittoral to bathyal environments and show significant changes in bottom-water oxygenation. Simultaneously, the occurrences of epiphytic damaged foraminifers and the blocks of Porites point out the presence of plant substrates and coral-reefs on the infralittoral zone. The overlying evaporitic unit is characterized by four subaqeous selenitic gypsum layers and three marine fossiliferous intercalations. Each episode of gypsum deposition ends with a desiccation event. Each fossiliferous intercalations traduce a rapid reflooding of the basin by marine waters corresponding to infralittoral to upper bathyal environments with normal marine conditions. During the evaporitic period, the ecological conditions in the water column were intermittently favorable to the development of marine organisms, and moreover, the marginal basins of the Betic Cordillera were not continuously desiccated during the Messinian. The low percentage of extinct species (5%) at the Messinian-Pliocene boundary and the occurrences of benthic foraminifers in the evaporitic unit indicate that the infralittoral to upper bathyal environments have not been completely modified and/or barren during the 'Messinian Salinity Crisis', i.e. the 'Messinian Salinity Crisis' is not associated with a biological crisis. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, Geol Lab, F-49045 Angers, France. Univ Rennes 1, Lab Paleontol, F-35042 Rennes, France. Natl Museum Nat Hist, Geol Lab, CNRS ESA 7073, F-75005 Paris, France. RP Goubert, E, Univ Bretagne Sud, Ctr Y Coppens, Campus Tohannie,Rue Yves Mainguy, F-56017 Vannes, France. TC 8 PD DEC 20 PY 2001 VL 175 IS 1-4 SI Sp. Iss. SI BP 61 EP 78 UT ISI:000173067900005 ER PT J AU Pagano, A Crochet, PA Graf, JD Joly, P Lode, T TI Distribution and habitat use of water frog hybrid complexes in France SO GLOBAL ECOLOGY AND BIOGEOGRAPHY DE amphibians; France; hybrid zone; mosaic zone model; Rana species; tension zone model; water frogs ID RANA-RIDIBUNDA; ESCULENTA; ZONE AB Hybrid zones are either distributed along clines or in a mosaic of patches. This distribution may depend upon variation in taxon habitat use. Habitat use and distribution of diverse taxa of water frogs (Rana ridibunda, R. lessonae, R. perezi, R. kl. grafi and R. kl. esculenta) in France are analysed to determine whether water frog complexes conform to the mosaic or clinal model. Biogeographical scenarios may be invoked in order to explain the distribution of water frogs. However, the distribution of R. perezi and R. kl. grafi, being restricted to regions characterized by Mediterranean or Oceanic climatic conditions, suggests that these frogs do not endure cold winters. R. ridibunda is widespread in Southern France and its distribution suggests multiple introductions. It is concluded that water frogs conform to the mosaic zone model rather than to the tension zone model because: (i) taxa exhibited differences in habitat use, (ii) pure parental species were documented and (iii) hybrids are not unfit relative to parental species. C1 Univ Angers, Lab Ecol Anim, F-49045 Angers, France. Stn Biol Tour Valat, F-13200 Arles, France. Univ Montpellier 2, Lab Genome Populat Interact, F-34095 Montpellier, France. Univ Lyon 1, Lab Ecol Hydrosyst, F-69622 Villeurbanne, France. Hop Cantonal Univ, Lab Cent Chim Clin, CH-1211 Geneva 14, Switzerland. RP Pagano, A, Univ Angers, Lab Ecol Anim, Campus Belle Beille,2 Bvd Lavoisier, F-49045 Angers, France. TC 8 PD JUL PY 2001 VL 10 IS 4 BP 433 EP 441 UT ISI:000172043300010 ER PT J AU Lode, T Cormier, JP Le Jacques, D TI Decline in endangered species as an indication of anthropic pressures: The case of European mink Mustela lutreola western population SO ENVIRONMENTAL MANAGEMENT DE decline; endangered species; habitat change; mustelid; Mustela lutreola ID LUTRA-LUTRA POPULATIONS; BLACK-FOOTED FERRETS; AMERICAN MINK; WILDLIFE CORRIDORS; EXTINCTION; CONSERVATION; VISON; GENETICS; FRANCE; DIET AB Populations of threatened species, especially predators at the top of the food chain, may be affected by anthropic pressures. The endangered western population of European mink Mustela lutreola has shown a large decline over 50% of its natural range. M. lutreola disappeared from northwestern France between 1984 and 1997, and the decline was associated with an increase in mustelid trapping, changes in watercourse quality, and habitat modifications due to agricultural practices. The pattern of decline showed a fragmentation restricting the minks into very small areas. Trapping was the first known cause of mortality. Although feral American mink Mustela vison may compete with autochthonous carnivores, M. lutreola had disappeared from streams before the introduction of the American species, suggesting that competitive interactions were not responsible. Furthermore, American mink has never been found or has remained rare in 62.4% of the area from which M, lutreola has disappeared. During the past 25 years, permanent grassland surfaces were reduced by 40%, whereas fodder culture increased by 470%, causing considerable habitat changes, Furthermore, 55.7% of water courses were classified as being of bad quality or polluted. Therefore, our data suggests that a conjunction of intensive trapping, alterations in water quality and habitat modification was critical for the European mink's decline. Although there are difficulties in ascribing specific cause to distribution changes in a top predator, this decline can be regarded as an indication for anthropic pressures on natural habitats. C1 Univ Angers, UFR Sci, Lab Ecol Anim, F-49045 Angers, France. RP Lode, T, Univ Angers, UFR Sci, Lab Ecol Anim, Campus Belle Beille,2 Bd Lavoisier, F-49045 Angers, France. TC 8 PD DEC PY 2001 VL 28 IS 6 BP 727 EP 735 UT ISI:000171990600003 ER PT J AU Colmont, JM D'Eu, JF Rohart, F Wlodarczak, G Buldyreva, J TI N-2- and O-2-broadenings and lineshapes of the 551.53-GHz line of (NO)-N-14 SO JOURNAL OF MOLECULAR SPECTROSCOPY DE NO; nitric oxide; line broadening; lineshape; collisional relaxation; millimeter spectroscopy ID WAVE COHERENT TRANSIENTS; NITRIC-OXIDE; BROADENING COEFFICIENTS; FUNDAMENTAL-BAND; SPEED DEPENDENCE; HIGH-RESOLUTION; NO; PARAMETERS; SHIFT; SELF AB For the purpose of atmospheric applications, the collisional relaxation of the J = 5.5 <-- 4.5 rotational line of (NO)-N-14 (551.53 GHz) has been investigated in the 235 to 345-K temperature range. Experiments were performed with N-2 and O-2 as buffer gases, which allow the derivation of the air-induced broadening parameters. Using a video-type spectrometer, small but significant departures from the usual Voigt profile have been demonstrated. These departures are characteristic of narrowed lineshapes: they have been fitted using the speed-dependent Galatry profile that takes account of both the dependence of relaxation rates on molecular speeds and the molecular diffusion via velocity changing collisions. Retrieved broadening parameters, as well as their temperature dependencies, are in agreement with previous determinations dealing with rovibrational lines: they are fairly reproduced by semi-classical calculations that consider exact collisional trajectories. The detailed lineshape properties have been studied thanks to some further experiments using atomic buffer gases, namely He, Ar, and Xe. If we discard the case of He-induced relaxation, theoretical arguments suggest that observed line narrowings are mainly related to the speed dependence of relaxation and that molecular diffusion plays a negligible role. (C) 2001 Academic Press. C1 Univ Sci & Tech Lille Flandres Artois, Lab Phys Lasers Atom & Mol, UMR 8523, CNRS,Ctr Etud & Rech Lasers & Applicat, F-59655 Villeneuve Dascq, France. Univ Angers, Lab Proprietes Opt Mat & Applicat, UMR 6136, CNRS, F-49045 Angers, France. RP Rohart, F, Univ Sci & Tech Lille Flandres Artois, Lab Phys Lasers Atom & Mol, UMR 8523, CNRS,Ctr Etud & Rech Lasers & Applicat, F-59655 Villeneuve Dascq, France. TC 8 PD AUG PY 2001 VL 208 IS 2 BP 197 EP 208 UT ISI:000170996200008 ER PT J AU Bellec, V De Backer, MG Levillain, E Sauvage, FX Sombret, B Wartelle, C TI In situ time-resolved FTIR spectroelectrochemistry: study of the reduction of TCNQ SO ELECTROCHEMISTRY COMMUNICATIONS DE electrochemistry; IR spectroscopy; spectroelectrochemistry; TCNQ ID RESONANCE RAMAN SPECTROELECTROCHEMISTRY; INFRARED SPECTROELECTROCHEMISTRY; TETRACYANOQUINODIMETHANE DIANION; CONCENTRATION PROFILES; UV-VIS; SPECTROSCOPY; MICROSCOPY; EXCITATION; DIFFUSION; LAYER AB The efficiency and versatility of time-resolved FTIR spectroscopy has been used to follow concentration profiles of species produced during a cyclic voltammetric scan. It has been tested in situ and in resolved time, by probing the reduction of tetracyanoquinodimethane (TCNQ) on its first and second electrochemical wave. Besides the establishment of the method, the individual concentrations of TCNQ, of the monoanion and of the dianion were monitored at distinct infrared frequencies and the time derivatives of the concentration profiles were compared to the voltammograms. (C) 2001 Elsevier Science BN. All rights reserved. C1 CNRS, UMR 8516, HEI, LASIR, F-59046 Lille, France. CNRS, UMR 6501, IMMO, F-49045 Angers, France. Univ Sci & Tech, CNRS, UMR 8516, USTL,LASIR, F-59650 Villeneuve Dascq, France. RP De Backer, MG, CNRS, UMR 8516, HEI, LASIR, 13 Rue Toul, F-59046 Lille, France. TC 8 PD SEP PY 2001 VL 3 IS 9 BP 483 EP 488 UT ISI:000170860100003 ER PT J AU Donal, E Abgueguen, P Coisne, D Gouello, JP McFadden, EP Allal, J Corbi, P TI Echocardiographic features of Candida species endocarditis: 12 cases and a review of published reports SO HEART DE echocardiography; Candida species; endocarditis ID PROSTHETIC VALVE ENDOCARDITIS; INFECTIVE ENDOCARDITIS; TRANSESOPHAGEAL ECHOCARDIOGRAPHY; RISK AB Objective-To describe the specific echocardiographic features of Candida species endocarditis. Design-Retrospective review of the case records of patients with confirmed candida endocarditis. Setting-Cases referred to three French university centres over an eight year period were studied. Design-12 patients with confirmed Candida species endocarditis infection were identified. The transthoracic (n = 12) and transoesophageal (n = 12) echocardiographic appearances were compared with the surgical findings (n = 10). Results-Large dense heterogeneous vegetations were found in 11/12 cases. A hyperechogenic heterogeneous myocardial texture, observed in seven of the 12 patients, was associated with extensive myocardial damage at surgery. While it was possible to diagnose candidal cardiac infection in all patients by transthoracic echocardiography, transoesophageal echo cardiography was useful for optimal assessment of the valvar and paravalvar structures. Conclusions-In the setting of endocarditis, the detection of myocardial involvement, which is characterised by a heterogeneous myocardial texture, is an argument in favour of Candida species endocarditis and may warrant early surgical intervention. C1 CHU La Miletrie, Dept Cardiol, F-86021 Poitiers 01, France. CHU Angers, Serv Malad Infect & Trop, F-49033 Angers, France. Hop Cardiovasc, F-69394 Lyon 03, France. RP Donal, E, CHU La Miletrie, Dept Cardiol, 350 Av Jacques Coeur,B P577, F-86021 Poitiers 01, France. TC 8 PD AUG PY 2001 VL 86 IS 2 BP 179 EP 182 UT ISI:000170107600020 ER PT J AU Hideur, A Chartier, T Ozkul, C Sanchez, F TI All-fiber tunable ytterbium-doped double-clad fiber ring laser SO OPTICS LETTERS ID MU-M; AMPLIFIER; OUTPUT; POWER; DIODE AB We have designed and realized a cw all-fiber tunable Yb-doped double-clad fiber laser in a unidirectional ring cavity. The side-pumping V-groove technique is used, yielding very good launching efficiency of the high-power pumping laser diode, together with a compact configuration. The fiber laser delivers as much as 800 mW of power and is tunable in the spectral range 1.04-1.10 mum. The laser linewidth is similar to0.1nm. (C) 2001 Optical Society of America. C1 Univ Rouen, Unite Mixte Rech 6614, Grp Opt & Optron, Complexe Rech Interprof & Aerothermochim, F-76821 Mt St Aignan, France. Univ Angers, Unite Mixte Rech 6136, Lab Proprietes Opt Mat & Applicat, F-49000 Angers, France. RP Hideur, A, Univ Rouen, Unite Mixte Rech 6614, Grp Opt & Optron, Complexe Rech Interprof & Aerothermochim, Pl Emile Blondel, F-76821 Mt St Aignan, France. TC 8 PD JUL 15 PY 2001 VL 26 IS 14 BP 1054 EP 1056 UT ISI:000169858800006 ER PT J AU Conreux, F Best, O Preckel, MP Lhopitault, C Beydon, L Pouplard, F Granry, JC TI Electroencephalographic effects of sevoflurane in paediatric anaesthesia: a prospective study of 20 cases. SO ANNALES FRANCAISES D ANESTHESIE ET DE REANIMATION DE anaesthesia; child; electroencephalogram; infant; myoclonic movement; sevoflurane ID PEDIATRIC-PATIENTS; NITROUS-OXIDE; ANESTHESIA; INDUCTION; HALOTHANE; CHILDREN; ENFLURANE; CONVULSIONS; ISOFLURANE; MOVEMENTS AB Objective: To study electroencephalographic (EEG) changes in children during induction of anaesthesia with 8% sevoflurane. Patients: Twenty patients were consecutively included (ASA I-II; aged : 13-101 months). Material and method: Prospective study; approved by the Ethics Committee;written informed consent obtained from parents; anaesthesia induced with 8% sevoflurane in oxygen; no premedication was given; to collect the EEG data, non-invasive electodes were installed before induction; EEG was recorded continuously and stored on a computer for later analyses (descriptive analysis). Results: Myoclonics movements were observed during induction of anaesthesia in two boys. Myoclonics movements stopped spontaneously without therapy. Epileptiform EEG activity (spikes and poly-spikes, burst suppression) was observed. In the 18 others cases, during sevoflurane induction, EEG changed rapidly with an increase in the range of beta activity (> 13 Hz) (n = 15) and in slow (< 8 Hz) and delta activity (< 4 Hz). In 14 patients, at time of laryngoscopy, the EEGs showed monophasic slow data activity (sharp high-voltage slow waves). Periods of EEG isoelectrical (burst suppression) were seen without spikes in four cases. Conclusion: At 2 MAC, epileptiform EEG activity has been observed during sevoflurane anaesthesia. In a short group of patients, this raises the question of avoiding sevoflurane in patients who have a history of epilepsy. (C) 2001 Editions scientifiques et medicales Elsevier SAS. C1 CHU Angers, Dept Anesthesie Reanimat, F-49033 Angers, France. RP Conreux, F, CHU Angers, Dept Anesthesie Reanimat, F-49033 Angers, France. TC 8 PD MAY PY 2001 VL 20 IS 5 BP 438 EP 445 UT ISI:000169238400002 ER PT J AU Ritz, P CA Source Study TI Bioelectrical impedance analysis estimation of water compartments in elderly diseased patients: The source study SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES ID TOTAL-BODY WATER; EXTRACELLULAR WATER; FLUID BALANCE; DEHYDRATION; VALIDATION; BROMIDE AB Background. This study validates, in geriatric patients, bioelectrical impedance analysis (BIA) equations that had been derived to estimate total body water (TBW) and extracellular water (ECW) in healthy elderly subjects. Methods. We performed a multicentric trial in six geriatric wards. We studied 169 patients with varying degrees of hydration: dehydrated, euvolemic, and overhydrated. BIA estimates of TBW and of ECW were compared with the measurement of TBW with O-18 dilution and of ECW with bromide (Br) dilution. Results. BIA estimated TBW with a difference of 0.48 +/- 2.31 (mean +/- SD) (50 kHz: p = .01) and 0.69 +/- 2.21 (100 kHz; p < 0.001) compared with O-18 dilution. The difference was not affected by the hydration status. Estimates of ECW with BLA were systematically biased compared with Br dilution: 4.6 +/- 3.11 (equation from Segal and colleagues: p < .001) and 3.4 +/- 2.91 (equation from Visser and colleagues; p < .001). We propose a new. cross-validated equation. Conclusions. Body water spaces can he estimated accurately in geriatric patients with BIA. C1 CHU Angers, Med Serv B, F-49033 Angers 01, France. RP Ritz, P, CHU Angers, Med Serv B, F-49033 Angers 01, France. TC 8 PD JUN PY 2001 VL 56 IS 6 BP M344 EP M348 UT ISI:000169124200011 ER PT J AU Ritz, P CA Ivest Source Study Human Nutr Res TI Chronic cellular dehydration in the aged patient SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES ID BIOELECTRICAL-IMPEDANCE ANALYSIS; TOTAL-BODY WATER; EXTRACELLULAR WATER; BROMIDE; YOUNG; WOMEN; MEN AB Background. As a reduction of water spaces is expected in the elderly because of fat-free mass loss, disease is often associated with increased hydration. The present study compared water spaces and cellular hydration in adults, healthy and diseased aged patients. Methods. An open study was conducted in 6 geriatric wards and a nutrition laboratory involving 85 aged diseased persons, 68 healthy elderly adults, and 35 adults. Total body water (TBW, (H2O)-O-18 dilution), extracellular water (ECW, Bromide dilution), and fat-free mass (FFM, body density and Siri's equation) were measured directly whereas intracellular water (ICW = TBW - ECW) and body cell mass (FFM - ECW) were obtained by calculations. Results. FFM, TBW, and ICW were higher in adults than in the 2 other groups and in the elderly than in aged patients. ECW was higher in aged patients than in healthy elderly participants. The proportion of TBW made of ECW or ICW was the same in adults and in healthy elderly persons. A higher proportion of TBW was composed of ECW, and a lower proportion of TBW was composed of ICW, in diseased patients compared with the 2 other groups. The proportion of ICW in body cell mass was also lower in diseased patients. Conclusions. Diseased elderly persons display reduced ICW and expanded ECW. A cellular dehydration is suggested. C1 CHU Angers, Serv Med B, F-49033 Angers 01, France. RP Ritz, P, CHU Angers, Serv Med B, F-49033 Angers 01, France. TC 8 PD JUN PY 2001 VL 56 IS 6 BP M349 EP M352 UT ISI:000169124200012 ER PT J AU Namer, M Soler-Michel, P Turpin, F Chinet-Charrot, P de Gislain, C Pouillart, P Delozier, T Luporsi, E Etienne, PL Schraub, S Eymard, JC Serin, D Ganem, G Calais, G Maillart, P Colin, P Trillet-Lenoir, V Prevost, G Tigaud, D Clavere, P Marti, P Romieu, C Wendling, JL TI Results of a phase III prospective, randomised trial, comparing mitoxantrone and vinorelbine (MV) in combination with standard FAC/FEC in front-line therapy of metastatic breast cancer SO EUROPEAN JOURNAL OF CANCER DE metastatic breast cancer ID ADJUVANT CHEMOTHERAPY; CLINICAL-TRIAL; DOXORUBICIN; CYCLOPHOSPHAMIDE; FLUOROURACIL; CARCINOMA; MULTICENTER; ADRIAMYCIN; EPIRUBICIN; 5-FLUOROURACIL AB This comparative phase III trial of mitoxantrone + vinorelbine (MV) versus 5-fluorouracil + cyclophosphamide + either doxorubicin or epirubicin (FAC/FEC) in the treatment of metastatic breast cancer was conducted to determine whether MV would produce equivalent efficacy, while resulting in an improved tolerance in relation to alopecia and nausea/vomiting. This multicentre study recruited and randomised 281 patients with metastatic breast cancer; 280 were evaluable for response survival and toxicity (138 received FAC/FEC, 142 received MV). Patient characteristics were matched in each arm and stratification for prior exposure to adjuvant therapy was made prospectively. The overall response rate (ORR) was equivalent in the two arms (33.3% for FAG, FEC versus 34.5% for MV), but MV was more effective in patients who had received prior adjuvant therapy (13% (95% confidence interval (CI) 3-23) for FAC/FEC versus 33% (95% CI 20 47) for MV P = 0.025) with a better progression-free survival (PFS) (5 months (range 1-18 months) versus 8 months (range 1-27 months); P = 0.0007 for FAC/FEC versus MV, respectively) while FAC/ FEC was more: effective in previously untreated patients (ORR 43% (95% CI 33 53) versus 35% (95% CI 25-45), P = 0.26; PFS 9 months (range 0-29 months) versus 6 months (range 0 - 26 months) P = 0.014). Toxicity was monitored through the initial six cycles of therapy; febrile neutropenia and delayed haematological recovery was more frequent for MV (P = 0.001), while nausea/vomiting of grades 3-4 was greater for FAC/FEC (P = 0.031), as was alopecia (P = 0.0001), cardiotoxicity was the same for the two regimens. MV represents a chemotherapy combination with equivalent efficacy to standard FAC/FEC and improved results for patients who have previously received adjuvant chemotherapy. Toxicity must be balanced to allow for increased haematological suppression and risk of febrile neutropenia with MV compared with a higher risk of subjectively unpleasant side-effects such as nausea/vomiting and alopecia with FAC/FEC. (C) 2001 Elsevier Science Ltd. All rights reserved. C1 Ctr Antoine Lacassagne, F-06002 Nice, France. RCTs, Biostat Unit, Lyon, France. Ctr Rene Huguenin, F-92210 St Cloud, France. Ctr Henri Becquerel, F-76038 Rouen, France. Ctr Georges Francois Leclerc, Dijon, France. Inst Curie, F-75005 Paris, France. Ctr Francois Baclesse, F-14021 Caen, France. Ctr Alexis Vautrin, F-54500 Vandoeuvre Les Nancy, France. Clin Armoricaine, St Brieuc, France. Hop Jean Minjoz, F-25030 Besancon, France. Inst Jean Godinoi, F-51056 Reims, France. Clin St Catherine, Avignon, France. Ctr Jean Bernard, Le Mans, France. CHU Tours, F-37033 Tours, France. Ctr Paul Papin, Angers, France. Polyclin Courlancy, Reims, France. Ctr Hosp Lyon Sud, F-69310 Pierre Benite, France. Ctr Hosp, Mulhouse, France. Hop Edouard Herriot, F-69374 Lyon, France. Dupuytren Hosp, Limoges, France. Policlin Med Trespoey, Pau, France. Ctr Paul Lamarque, Montpellier, France. Hop St Louis, Toulon, France. RP Namer, M, Ctr Antoine Lacassagne, 36 Voie Romaine, F-06002 Nice, France. TC 8 PD JUN PY 2001 VL 37 IS 9 BP 1132 EP 1140 UT ISI:000169231700021 ER PT J AU Giffard, H Mabon, G Leclair, E Mercier, N Allain, M Gorgues, A Molinie, P Neilands, O Krief, P Khodorkovsky, V TI Oxidation of TTF derivatives using (Diacetoxyiodo)benzene: a general chemical route toward cation radicals, dications, and nonstoichiometric salts SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY ID TETRATHIAFULVALENE TTF; ELECTRON-DONORS; CHEMISTRY C1 Univ Angers, Lab Ingn Mol & Mat Organ, UMR 6501, CNRS, F-49045 Angers, France. Univ Nantes, Inst Mat Nantes, UMR 6510, CNRS, F-44072 Nantes, France. Riga Tech Univ, Dept Organ Chem, LV-1048 Riga, Latvia. Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel. RP Giffard, H, Univ Angers, Lab Ingn Mol & Mat Organ, UMR 6501, CNRS, 2 Bld Lavoisier, F-49045 Angers, France. TC 8 PD APR 25 PY 2001 VL 123 IS 16 BP 3852 EP 3853 UT ISI:000168442500039 ER PT J AU Beydon, L Conreux, F Le Gall, R Safran, D Cazalaa, JB CA Commission Materiovigilance Anaest TI Analysis of the French health ministry's national register of incidents involving medical devices in anaesthesia and intensive care SO BRITISH JOURNAL OF ANAESTHESIA DE equipment, anaesthesia machines; equipment, failure; monitoring, intensive care ID CLOSED CLAIMS ANALYSIS; ANESTHESIA; PREVENTION; VIGILANCE; PIPELINE; MISHAPS; FAILURE; SAFETY AB This study details all incidents involving medical devices used in anaesthesia and intensive care reported to the relevant authorities in France in 1998. There were 1004 reports during that year. Incidents were classified as serious (harmful to patients) in 11% of cases; death resulted in 2% of cases. Equipment for ventilation and infusion, and monitors of all kinds, accounted for most of the reports, representing 37%, 30% and 12%, respectively, of all reports. The leading causes of failure varied according to the category of device. User errors, quality control problems during production of the device and design faults were the three main causes. The problems identified during the study period enabled the faulty medical devices to be improved in 12-44% of cases. We conclude that post-marketing vigilance is a useful way of improving the quality of medical devices. C1 CHU Angers, Dept Anaesthesie, F-49033 Angers 01, France. Hop Laennec, Dept Anaesthesie, F-75007 Paris, France. Hop Necker Enfants Malad, Dept Anaesthesie, F-75015 Paris, France. RP Beydon, L, CHU Angers, Dept Anaesthesie, F-49033 Angers 01, France. TC 8 PD MAR PY 2001 VL 86 IS 3 BP 382 EP 387 UT ISI:000167443900012 ER PT J AU Fanello, S Bouchara, JP Jousset, N Delbos, V LeFlohic, AM TI Nosocomial Candida albicans acquisition in a geriatric unit: epidemiology and evidence for person-to-person transmission SO JOURNAL OF HOSPITAL INFECTION DE Candida albicans; hospital-acquired infection; elderly patients; pulsed field gel electrophoresis ID INTENSIVE-CARE UNIT; DIABETES-MELLITUS; FUNGAL-INFECTIONS; MITOCHONDRIAL-DNA; ORAL CANDIDIASIS; TYPING METHODS; STRAINS; OUTBREAK; ELECTROPHORESIS; POLYMORPHISM AB This prospective study, which included 69 patients over a two-month period, was conducted to evaluate the incidence of community-acquired carriage and hospital-acquired yeasts in elderly patients hospitalized in a short stay care unit. Furthermore, possible person-to-person transmission was investigated by means of genotyping the Candida albicans isolates obtained from samples (throat swabs, urine and stools) systematically taken from the patients at different times during the hospitalization and from healthcare workers (throat swabs and handwashes) in the middle of the observation period. This study showed a high prevalence rate of 64% for community-acquired yeast carriage and a 40% incidence rate for hospital-acquired yeasts. Mycological analysis of the samples from the healthcare workers revealed eight of them to be colonized in the oral cavity: In addition, pulsed field gel electrophoresis and mitochondrial DNA analysis of the C. albicans isolates demonstrated person-to-person transmission. This study suggests that regular mycological sampling should be done as hospitalized elderly patients are frequently colonized by yeasts. Likewise, oropharyngeal swabs from healthcare workers may be helpful in this setting. (C) 2001 The Hospital Infection Society. C1 UFR Med, Dept Sante Publ, F-49045 Angers, France. Ctr Hosp Univ, Lab Parasitol Mycol, GEIHP, Angers, France. CHU Brest, Dept Microbiol, F-29285 Brest, France. RP Fanello, S, UFR Med, Dept Sante Publ, Rue Haute de Reculee, F-49045 Angers, France. TC 8 PD JAN PY 2001 VL 47 IS 1 BP 46 EP 52 UT ISI:000166680900010 ER PT J AU Neraudeau, D Goubert, E Lacour, D Rouchy, JM TI Changing biodiversity of Mediterranean irregular echinoids from the Messinian to the Present-Day SO PALAEOGEOGRAPHY PALAEOCLIMATOLOGY PALAEOECOLOGY DE neogene; Messinian; Mediterranean; marine biodiversity; irregular echinoids; Schizaster ID SALINITY CRISIS; SOUTHEASTERN SPAIN; SORBAS BASIN; LORCA BASIN; CONSTRAINTS; SPATANGOIDA; GYPSUM; SEA AB Morphological and palaeoecological changes in irregular echinoid diversity during the Late Miocene Messinian stage have been analysed at the scale of the Sorbas Basin of southeast Spain. Biometrical and statistical analyses established the validity of species differentiation. An example is given for Messinian. Pliocene and Recent Schizaster species: S. saheliensis and S. canaliferus. Palaeoecological analyses show that irregular echinoid faunas record environmental changes, especially sea-level variations, with contrasting ecomorphological gradients for deepening and shallowing trends. Deepening is indicated by the stratigraphic succession of Clypeaster altus, associated Clypeaster marginatus-Spatangus-Schizaster and then Brissopsis species whereas shallowing is indicated by the opposite succession. The survival of Brissopsis, typically a stenohaline and relatively deep marine organism, in the marls interbedded within the uppermost Messinian gypsum of the Sorbas Basin testifies to the existence of marine connexions between this basin and other Mediterranean marine areas during the end of the evaporitic episode. Tabulation of irregular echinoid species diversity at eight time intervals between the Tortonian/Messinian boundary and the present-day (inclusive) for the Mediterranean Basin as a whole indicates a progressive increase in diversity from the latest Tortonian to the upper part of the pre-evaporitic Messinian before a fall in diversity during the salinity crisis. However, survivals after the crisis are very numerous, representing 78% of the maximum pre-evaporitic value, (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Rennes 1, Lab Paleontol, F-35042 Rennes, France. Univ Angers, Geol Lab, F-49045 Angers, France. Museum Natl Hist Nat, Geol Lab, CNRS ESA 7073, F-75005 Paris, France. RP Neraudeau, D, Univ Rennes 1, Lab Paleontol, Ave Gen Leclerc, F-35042 Rennes, France. TC 7 PD DEC 20 PY 2001 VL 175 IS 1-4 SI Sp. Iss. SI BP 43 EP 60 UT ISI:000173067900004 ER PT J AU Bagwell, CB Clark, GM Chassevent, A Bendahl, PO Stal, O Killander, D Jourdan, ML Romain, S Hunsberger, B Wright, S Baldetorp, B TI DNA and cell cycle analysis as prognostic indicators in breast tumors revisited SO CLINICS IN LABORATORY MEDICINE ID PARAFFIN-EMBEDDED TISSUE; FALSE ANEUPLOID PEAKS; S-PHASE FRACTION; FLOW-CYTOMETRY; SOLID TUMORS; PROLIFERATIVE ACTIVITY; STAGE-I; CANCER; PLOIDY; AUTOLYSIS AB Both DNA ploidy and S-phase ploidy are promising prognostic factors for node-negative breast cancer patients. Based largely on the analysis of one large study, much of the reported problems with these factors have been caused by some unappreciated complexities in categorizing DNA ploidy into low- and high-risk groups and the lack of some necessary adjustments to eliminate unwanted correlations between DNA S-phase and ploidy. When both DNA ploidy and S-phase are compensated properly, they become independent prognostic markers, forming a powerful prognostic model. C1 Ver Software House Inc, Topsham, ME 04086 USA. Baylor Coll Med, Breast Ctr, Houston, TX 77030 USA. Ctr Rene Huguenin, St Cloud, France. Ctr Paul Papin, Angers, France. Univ Lund Hosp, Jubileum Inst, Dept Oncol, S-22185 Lund, Sweden. Linkoping Univ, Fac Hlth Sci, Dept Oncol, Linkoping, Sweden. Hop Bretonneau, Tours, France. Assistance Publ Hop Marseille, Marseille, France. Esoterix Oncol, Brentwood, TN USA. RP Bagwell, CB, Ver Software House Inc, POB 247, Topsham, ME 04086 USA. TC 7 PD DEC PY 2001 VL 21 IS 4 BP 875 EP + UT ISI:000172773000013 ER PT J AU Malzert, A Boury, F Saulnier, P Benoit, JP Proust, JE TI Interfacial properties of a PEG2000-PLA50 diblock copolymer at the air/water interface SO LANGMUIR ID AIR-WATER-INTERFACE; POLY(ETHYLENE OXIDE) MONOLAYERS; DILATATIONAL PROPERTIES; TRIBLOCK COPOLYMERS; POLYMER MONOLAYERS; PEO; MICROSPHERES; RELEASE; SPREAD; ENCAPSULATION AB In this paper, we compared the interfacial behaviors of spread films of poly(ethylene glycol) (PEG2000), poly(D,L-lactide) (PLA50), and a mixture of PEG2000 and PLA50, to understand the properties of a PEG2000-PLA50 diblock copolymer at the air/water interface. This was achieved (i) by analyzing the surface pressure-surface area curves obtained on a Langmuir trough and (ii) by modeling the dilatational properties of the films according to a modified Maxwell model. The properties of the films composed of a mixture of PEG2000 and PLA50 showed the influence of PEG2000 before the nucleation transition of PLA50. Then, increasing the lateral compression of the mixed monolayer led to the expulsion of PEG2000 segments with presumably their irreversible desorption into the bulk. In the case of the copolymer, the covalently bound PEG2000 segments were segregated at low surface coverage forming a stable film with the PEG corona oriented toward the water phase. This situation was conserved until high surface coverage (10-15 mN/m). Afterward, compression led to the penetration of PEG2000 into the tridimensional layer oriented toward the air/phase. These results allow better understanding and modulation of the composition and the hydrophilic character of interfaces formed during emulsion processes and thus improvement of the control of the surface properties of drug delivery systems. C1 Immeuble IBT, INSERM ERIT M 0104, F-49100 Angers, France. RP Boury, F, Immeuble IBT, INSERM ERIT M 0104, 10 Rue Andre Boquel, F-49100 Angers, France. TC 7 PD DEC 11 PY 2001 VL 17 IS 25 BP 7837 EP 7841 UT ISI:000172590800020 ER PT J AU Pouliquen, D Gallois, Y TI Physicochemical properties of structured water in human albumin and gammaglobulin solutions SO BIOCHIMIE DE water; serumalbumin; gammaglobulin; NMR; paramagnetic effect; correlation time ID NUCLEAR-MAGNETIC-RESONANCE; LIQUID TRANSITION; RELAXATION-TIME; BOUND WATER; PROTON NMR; HYDRATION; STATE; MOLECULES; TISSUES; MUSCLE AB The physicochemical properties of the different phases of water molecules were studied in concentrated solutions (132 g/L) of human serum albumin and gammaglobulin by H-1 NMR relaxometry. Spin-lattice (T1) relaxation times of total water and structured water (non-freezable water) were measured at 40 MHz above and below the freezing point of bulk water (ordinary, liquid water) at different temperatures. Analysis of the temperature dependence of the T1 demonstrated that total water differed qualitatively while structured water characteristics changed both quantitatively and qualitatively in the two protein solutions. Comparison of the temperature dependence of the structured water's Tl in the two solutions in the presence of an increasing concentration of manganese chloride allowed two main conclusions to be drawn. Firstly, the differences observed in total water and structured water physicochemical properties are directly related to protein structure and three-dimensional arrangement. Secondly, the motion of structured water determines the motion of the total water in the system through the values of the translational diffusion and chemical exchange correlation times tau (D) and taum. (C) 2001 Societe francaise de biochimie et biologie moleculaire / Editions scientifiques et medicales Elsevier SAS. All rights reserved. C1 Fac Med, Emi 00 18 Inserm, Lab Biochim & Biol Mol, F-49045 Angers, France. RP Pouliquen, D, Fac Med, Emi 00 18 Inserm, Lab Biochim & Biol Mol, Rue Haute Reculee, F-49045 Angers, France. TC 7 PD SEP PY 2001 VL 83 IS 9 BP 891 EP 898 UT ISI:000172210500005 ER PT J AU Fustec, J Lode, T Le Jacques, D Cormier, JP TI Colonization, riparian habitat selection and home range size in a reintroduced population of European beavers in the Loire SO FRESHWATER BIOLOGY DE aquatic vertebrate; dispersal; reintroduced species; territory size; willow grove ID CASTOR FIBER; CLASSIFICATION-SYSTEM; TRANSLOCATION; CANADENSIS; LANDSCAPE; IMPACT; FOREST; BASIN; TIME AB 1. Colonization in a reintroduced population of European beavers in the Loire Valley was studied between 1974 and 1999. It followed a discontinuous remoteness model and a scattered distribution, beavers occupying only 25% of the river system over the 2800 km explored. 2. After 5 years, the colonization rate reached 104.2% year(-1) of new sites occupied (SD 75% year(-1)), before dropping over the next 20 years. Nevertheless, the number of new colonies per km (0.125) remained stable throughout the years. 3. Populus nigra, Salix alba and Fraxinus angustifolia were the dominant woody species in beaver sites, often associated with some herbaceous species. 4. The length of willow grove dominated by S. alba and P. nigra (x) was the best predictor of beaver home range (y), fitting the equation y = -0.742x + 5.9. Long-term maintenance of the population requires a minimum of 1.79-km of willows per colony. 5. In cutting tree trunks, beavers stimulate shoot development from the remaining stumps. They rejuvenate riparian forests, increase the number of tree stems and help stabilize the banks. Their effect on woody plant morphogenesis may have consequences for the helophyte communities used as food or habitat by other aquatic species. C1 UFR Sci, Lab Ecol Anim, F-49045 Angers, France. ERMINEA, Nantes, France. RP Fustec, J, UFR Sci, Lab Ecol Anim, 2 Bd Lavoisier, F-49045 Angers, France. TC 7 PD OCT PY 2001 VL 46 IS 10 BP 1361 EP 1371 UT ISI:000172030800007 ER PT J AU Zouhair, R Defontaine, A Ollivier, C Cimon, B Symoens, F Hallet, JN Deunff, J Bouchara, JP TI Typing of Scedosporium apiospermum by multilocus enzyme electrophoresis and random amplification of polymorphic DNA SO JOURNAL OF MEDICAL MICROBIOLOGY ID POLYMERASE CHAIN-REACTION; CANDIDA-ALBICANS; ASPERGILLUS-FUMIGATUS; PSEUDALLESCHERIA-BOYDII; CRYPTOCOCCUS-NEOFORMANS; CLINICAL-SIGNIFICANCE; GENETIC DIVERSITY; CYSTIC-FIBROSIS; HYBRIDIZATION; STRAINS AB The genetic diversity among epidemiologically unrelated strains of the human pathogenic fungus Scedosporium apiospermum or its teleomorph, Pseudallescheria boydii, from different areas in Europe, was investigated by multilocus enzyme electrophoresis (MLEE) and random amplification of polymorphic DNA (RAPD). Fourteen enzyme activities were analysed by starch gel electrophoresis, corresponding to 27 polymorphic loci and 43 iso-enzymes. Among the enzymes studied, propionate esterase, carboxyl esterase, superoxide dismutase, carbonate dehydratase and malate dehydrogenase were the most polymorphic, allowing the classification of the strains into 6-11 groups each. Combination of the data obtained for the different enzyme activities studied allowed differentiation of the strains. Similarly, a high polymorphism was also revealed by each of the 20 RAPD primers tested, but no single primer was able to differentiate all the strains. The most efficient primers were GC70, UBC-701 and UBC-703, which revealed 17 distinct genotypes each, and combination of the results obtained with this three-primer set allowed complete discrimination of the strains. The dendrograms obtained from MLEE or RAPD by the unweighted pair-group method using arithmetic average cluster analysis did not reveal any clustering according to the geographic origin of the strains or their pathogenicity. C1 CHU Angers, UPRES EA 3142, Grp Etud Interact Hote Parasite, Lab Parasitol Mycol, Angers, France. Inst Sci Sante Publ Louis Pasteur, Sect Mycol, Brussels, Belgium. UMR CNRS 6553, Lab Parasitol Pharmaceut, Rennes, France. FRE CNRS 2230, Unite Biocatalyse, Biotechnol Lab, Nantes, France. RP Bouchara, JP, CHU Angers, UPRES EA 3142, Grp Etud Interact Hote Parasite, Lab Parasitol Mycol, Angers, France. TC 7 PD OCT PY 2001 VL 50 IS 10 BP 925 EP 932 UT ISI:000171322900013 ER PT J AU Rondeau, D Kreher, D Cariou, M Hudhomme, P Gorgues, A Richomme, P TI Electrolytic electrospray ionization mass spectrometry of C-60-TTF-C-60 derivatives: high-resolution mass measurement and molecular ion gas-phase reactivity SO RAPID COMMUNICATIONS IN MASS SPECTROMETRY ID <60>FULLERENE; SPECTRA AB The electrochemical process involved in electrospray ionization is used to obtain odd-electron molecular ions from C-60-TTF-C-60 and its methano derivatives. Exact mass measurements obtained using high-resolution mass spectrometry are reported, and the gas-phase behavior of the radical cation (retro-Diels-Alder reaction and [M/2 + H](+) ion formation) is described. Copyright (C) 2001 John Wiley & Sons, Ltd. C1 Univ Angers, Serv Commun Anal Spectroscop, F-49045 Angers, France. Univ Angers, Lab Ingn Mol & Mat Organ, CNRS, UMR 6501, F-49045 Angers, France. RP Rondeau, D, Univ Angers, Serv Commun Anal Spectroscop, 2 Bd Lavoisier, F-49045 Angers, France. TC 7 PY 2001 VL 15 IS 18 BP 1708 EP 1712 UT ISI:000171017900008 ER PT J AU Rochetaing, A Barbe, C Kreher, P TI Beneficial effects of amiodarone and dronedarone (SR 33589b), when applied during low-flow ischemia, on arrhythmia and functional parameters assessed during reperfusion in isolated rat hearts SO JOURNAL OF CARDIOVASCULAR PHARMACOLOGY DE ischemia and reperfusion; action potential; functional recovery; coronary flow; amiodarone; dronedarone ID VENTRICULAR-FIBRILLATION; GUINEA-PIG; ANTIARRHYTHMIC DRUGS; MYOCARDIAL-ISCHEMIA; CORONARY FLOW; MECHANISMS; MYOCYTES; CHANNELS; AGENTS; RABBIT AB The effects of short-term amiodarone and dronedarone treatments on action potential characteristics and arrhythmia (ventricular tachycardia) induced by reperfusion after global low-flow ischemia were studied in rat hearts. The actions of amiodarone and SR on recovery of coronary flow and contractile function were also determined. Isolated hearts were stabilized for 40 min and were then submitted to 25-min global low-flow ischemia (constant coronary flow, 0.3 ml/min) followed by 30 min of reperfusion at constant pressure. Drugs were applied only during ischemia: consequently, action potential duration (APD) tended to widen. During reperfusion, APD tended to recover or shorten, and the more complete the recovery, the less the arrhythmia. Despite its ability to widen APD during ischemia, amiodarone facilitated APD recovery during reperfusion. Moreover, APD shortening and ventricular tachycardia suppression exhibit a bell-shaped concentration-response relation, implying that the drugs affect ventricular tachycardia by a class III-independent action. These results point to an anti-ischemic action supported by improvement in function and inhibition of reactive hyperemia. C1 Univ Angers, UFR Sci, Precondit & Myocardium Remodeling Lab, F-49045 Angers, France. RP Rochetaing, A, Univ Angers, UFR Sci, Precondit & Myocardium Remodeling Lab, 2 Blvd Lavoisier, F-49045 Angers, France. TC 7 PD OCT PY 2001 VL 38 IS 4 BP 500 EP 511 UT ISI:000171062800002 ER PT J AU Cerbah, M Mortreau, E Brown, S Siljak-Yakovlev, S Bertrand, H Lambert, C TI Genome size variation and species relationships in the genus Hydrangea SO THEORETICAL AND APPLIED GENETICS DE Hydrangea; Hydrangeaceae; DNA content; chromosome number; karyotype; phylogeny ID FLOW CYTOMETRIC ANALYSIS; HORTENSIA LAM. REHD.; NUCLEAR-DNA CONTENT; ARBORESCENS L; SEQUENCES; EVOLUTION; ORGANIZATION AB Genome size and base composition in 16 species and subspecies of the Hydrangea, a woody ornamental genus of Hydrangeaceae, were evaluated by flow cytometry in relation to their chromosome number. This is the first such study concerning the genome size of these species together with a karyotype study of the most important species, Hydrangea macrophylla subsp. macrophylla (Hortensia), from an economical point of view. The 2C DNA content ranged from 1.95 pg in Hydrangea quercifolia to 5.00 pg in Hydrangea involucrata. The base composition ranged from 39.9% GC in Hydrangea aspera subsp. sargentiana to 41.1% in Hydrangea scandens subsp. scandens (significant difference at p < 0.05). The smallest genome sizes were those of the three species originating from North or South America. Most of the species studied presented a chromosome number of 2n = 2x = 36, except for those of the section Aspereae which showed 2n = 30, 34 and 36. A primary karyotype has been made for the first time for H. macrophylla subsp. macrophylla. Phylogenetic relationships between species, the origin of chromosome number and an exploration of the genetic diversity within the genus are discussed. C1 Univ Paris 11, CNRS UPRESA 8079, ESE, Lab Evolut & Systemat, F-91405 Orsay, France. Inst Natl Hort, F-49045 Angers, France. Inst Sci Vegetales, CNRS UP 40, F-91198 Gif Sur Yvette, France. RP Cerbah, M, Univ Paris 11, CNRS UPRESA 8079, ESE, Lab Evolut & Systemat, Bat 425, F-91405 Orsay, France. TC 7 PD JUL PY 2001 VL 103 IS 1 BP 45 EP 51 UT ISI:000170129000006 ER PT J AU Tuech, JJ Pessaux, P Regenet, N Rouge, C Hennekinne, S Bergamaschi, R Arnaud, JP TI Laparoscopic colectomy for sigmoid diverticulitis: A prospective study in the elderly SO HEPATO-GASTROENTEROLOGY DE laparoscopy; colon; elderly; sigmoid diverticulitis; high-risk patients ID COLORECTAL SURGERY; CHOLECYSTECTOMY; RISK; PNEUMOPERITONEUM; HEMODYNAMICS; AGE AB Background/Aims: The aim of this prospective study was to determine the feasibility and the complications or benefits of laparoscopic colectomy for sigmoid diverticulitis in patients aged 75 years or more. Methodology: From January 1993 to December 1999, 85 patients underwent an elective colectomy for sigmoid diverticulitis. Twenty-two patients over 75-years old (group 1) were compared to 63 younger patients (group 2). Results: In group 1, there were 12 women and 10 men, with a mean age of 77.2 years (range: 75-82); In group 2, there were 35 women and 28 men, with a mean age of 53.7 years (range: 38-74) (P=1.10-14). The operative time was shorter in group 2 (183 vs. 234min). There was no difference between the 2 groups with regard to the postoperative period during which parenteral analgesics were required (5.4 vs. 5.2 days, P=0.48) and the postoperative morbidity (18% vs. 14%, P=0.06). Postoperative length of hospital stay (13.1 vs. 8.8 days, P=0.003) was shorter in group 2 than in group 1. There was no perioperative mortality. Conversion rate was 9% (group 1) and 6% (group 2) (P=0.6). Conclusions: In summary, data from the present study suggest that laparoscopic colectomy for sigmoid diverticulitis can be applied safely to older patients with fewer complications, less pain, shorter hospital stay and a rapid return to preoperative activity levels. C1 Angers Univ Hosp, Dept Digest Surg, F-49000 Angers, France. RP Arnaud, JP, Angers Univ Hosp, Dept Digest Surg, 4 Rue Larrey, F-49000 Angers, France. TC 7 PD JUL-AUG PY 2001 VL 48 IS 40 BP 1045 EP 1047 UT ISI:000170175500033 ER PT J AU de Crescenzo, MAP Gallais, S Leon, A Laval-Martin, DL TI Tween-20 activates and solubilizes the mitochondrial membrane-bound, calmodulin dependent NAD(+) kinase of Avena sativa L. SO JOURNAL OF MEMBRANE BIOLOGY DE calmodulin-dependent NAD(+) kinase; K-m; mitochondrial membranes; solubilization; Tween-20; V-max ID ACHLOROPHYLLOUS ZC MUTANT; KLEBS STRAIN-Z; EUGLENA-GRACILIS; ENHANCED ACTIVITY; NADP PHOSPHATASE; DORMANT SEEDS; H+-ATPASE; ROOT-TIPS; STRESS; CALCIUM AB Among different treatments assayed, a mix of a nonionic detergent (5% Tween-20) with 0.5 M NaCl was found to solubilize a large part of the calmodulin dependent NAD(+) kinase bound to the inner mitochondrial membrane. It also stimulated its activity by increasing 7 times the maximal velocity. Activity stimulation was also observed with phosphatidylcholine, phosphatidylethanolamine and with reductants (HSO3 and DTT). This solubilized NAD(+) kinase and the calmodulin-dependent cytosoluble isoform displayed distinct molecular masses, as well as different kinetic parameters. We propose that solubilization of membrane-bound NAD(+) kinase could occur in vivo in Avena sativa and could generate a soluble isoform. C1 Univ Angers, Equipe Biochim & Biol Mol, UFR Sci, F-49045 Angers 01, France. RP de Crescenzo, MAP, Univ Angers, Equipe Biochim & Biol Mol, UFR Sci, 2 Blvd Lavoisier, F-49045 Angers 01, France. TC 7 PD JUL 15 PY 2001 VL 182 IS 2 BP 135 EP 146 UT ISI:000169944900005 ER PT J AU Penisson-Besnier, I Moreau, C Jacques, C Roger, JC Dubas, F Reynier, P TI Multiple sclerosis and Leber's hereditary optic neuropathy mitochondrial DNA mutations. SO REVUE NEUROLOGIQUE ID LHON MUTATIONS; ASSOCIATION; HAPLOTYPE; NEURITIS; DISEASE; GENOME; NERVE AB Multiple sclerosis (MS) has long been known to be associated with Leber's hereditary optic neuropathy (LHON), a disease caused by mitochondrial DNA (mtDNA) mutations. We have investigated the possible involvement of LHON mtDNA point mutations in MS. The study covered a group of 75 unrelated Caucasian patients, with the relapse-remitting or primary progressive form of MS, and a control group of 75 volunteers (matched for age, gender and ethnic origin). Mitochondrial DNA from each subject was examined for 4 primary LHON mutations (at nucleotide positions 3460, 4160, 11778 and 14484) and 7 secondary LHON mutations (at nucleotide positions 4216, 4917, 5244, 7444, 13708, 15257 and 15812) by means of restriction site polymorphism and sequencing techniques. None of the primary LHON mutations were detected in the MS patients or in the controls, whereas the proportion of individuals with secondary LHON mutations was identical (27p. cent) in the two groups. A combination of 2 or 3 homoplasmic mutations, defining mtDNA haplogroups, was found in the majority of cases. Haplogroups J, T and X were not particularly associated with MS. The frequency of the 13708 mutation alone (haplogroup X), or associated with the 4216 mutation (haplogroup J), was somewhat higher (p = 0.059) in the subgroup of MS patients with optic neuritis (ON). ON was the initial symptom in all but one of the patients with haplogroups J or X. No other correlation was found between MS phenotypes and mtDNA genotypes. Our observations confirm previous reports that neither primary nor secondary LHON mutations are involved in the development of MS. However, MS patients with haplogroups J or X appear to have a moderately higher risk of developing optic neuritis. Thus, a specific mtDNA background may be a predisposing genetic factor for optic nerve damage in MS patients. C1 CHU Angers, Hop Larrey, Dept Neurol, F-49033 Angers 01, France. Ctr Hosp Univ, Lab Biochim & Biol Mol, Angers, France. RP Penisson-Besnier, I, CHU Angers, Hop Larrey, Dept Neurol, 4 Rue Larrey, F-49033 Angers 01, France. TC 7 PD MAY PY 2001 VL 157 IS 5 BP 537 EP 541 UT ISI:000169503700007 ER PT J AU Delhaye, M Menei, P Rousselet, MC Diabira, S Mercier, P TI A case of intramedullary primary melanocytic tumor: meningeal melanocytoma or malignant melanoma? SO NEUROCHIRURGIE DE leptomeningeal tumor; meningeal melanocytoma; spinal cord tumor AB We report case of an intramedullary thoracic primary melanocytic tumor. Of the leptomeninges in a 38-year-old woman. According to the WHO classification, the lesion showed the histological features of a meningeal melanocytoma. Two local recurrences were successively operated on, two and three years after the first surgery respectively. The patient died of a subarachnoidian dissemination after a fours year coarse. C1 CHU Angers, Serv Neurochirurg, F-49033 Angers 01, France. CHU Angers, Serv Anat Pathol, F-49033 Angers, France. RP Menei, P, CHU Angers, Serv Neurochirurg, Rue Larrey, F-49033 Angers 01, France. TC 7 PD MAY PY 2001 VL 47 IS 2-3 PN Part 1 BP 133 EP 136 UT ISI:000169112600008 ER PT J AU Risede, JM Simoneau, P TI Typing Cylindrocladium species by analysis of ribosomal DNA spacers polymorphism: Application to field isolates from the banana rhizosphere SO MYCOLOGIA DE Calonectria; IGS; ITS; PCR-RFLP; taxonomy ID INTERNAL TRANSCRIBED SPACERS; RNA GENE; CALONECTRIA; MORPHOLOGY; PCR; AMPLIFICATION; SEQUENCES; FUNGI; IDENTIFICATION; ARMILLARIA AB Forty-four unidentified isolates of Cylindrocladium were recovered from banana root lesions and soils from intensive crapping systems in the Caribbean region, Costa Rica and Cameroon. They were then examined and compared with reference isolates using combinations of morphological characters, sexual crosses and polymorphism; of amplified rDNA spacers. According to conidium and vesicle morphology, they were consistently classed into two distinct morphotypes, MT1 and MT2. Sequences of PCR products of their internal transcribed spacer (ITS) region compared with similar sequences from reference isolates representing 7 species of Cylindrocladium revealed a very low polymorphism. By contrast, polymorphism in the amplified intergenic spacer region (IGS), as revealed by RFLP analysis, was found to be consistent with the current taxonomy of Cylindrocladium species, and sufficient to distinguish taxa at the inter- and intraspecific level. Using this IGS typing procedure, MT1 field isolates were found to group unequivocally with Cy. gracile, while MT2 isolates grouped with Cy. spathiphylli. The existence of intraspecific variation in the latter species was clearly demonstrated. Reports of these two species from the banana rhizosphere were confirmed by sexual crosses for Cy. spathiphylli, but not for Cy. gracile, which presently has no known teleomorph. IGS-RFLP markers therefore proved to constitute a rapid and suitable complement to morphological and mating studies for delineation of Cylindrocladium species. C1 CIRAD FLHOR, Lab Pathol Vegetale, Stn Neufchateau, Capesterre Belleeau 97130, Guadeloupe. Univ Angers, Fac Sci, UMR Pathol Vegetale, F-49045 Angers, France. RP Risede, JM, CIRAD FLHOR, Lab Pathol Vegetale, Stn Neufchateau, Capesterre Belleeau 97130, Guadeloupe. TC 7 PD MAY-JUN PY 2001 VL 93 IS 3 BP 494 EP 504 UT ISI:000168935100010 ER PT J AU Royer, A Menand, M Grimault, A Communal, PY TI Development of automated headspace gas chromatography determination of dithiocarbamates in plant matrixes SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY DE dithiocarbamates; automated headspace gas chromatography; plant matrixes; fungicides AB The determination of dithiocarbamates in plant matrixes is generally carried out by spectrophotometric (European Norm EN 12396-1, 1996) or gas chromatography headspace (European Norm EN 12396-2, 1999) methods; However, the former method presents a risk of carbon disulfide loss during hydrolysis and distillation and its sensitivity is low, whereas the latter method is time-consuming. In comparison to these European methods and in compliance with norm VO5-110, we have developed an automated gas chromatography headspace method. This method offers a good level of accuracy and precision and is specific to the compound determined (CS2). The limit of detection is below 0.020 mg/kg and the limit of quantification is below 0.050 mg/kg. Moreover, the recovery rates are between 85 and 103% with RSD less than 20%. The automated headspace method has several advantages when compared to the spectrophotometric and manual headspace methods, including the reduction of reagents employed for extraction and a greater number of analyses achievable per day than the other methods (approximate to 40 samples of food). C1 Grp Interreg Rech Prod Agropharmaceut, F-49070 Beaucouze, France. RP Communal, PY, Grp Interreg Rech Prod Agropharmaceut, Angers Technopole,8 Rue H Becquerel, F-49070 Beaucouze, France. TC 7 PD MAY PY 2001 VL 49 IS 5 BP 2152 EP 2158 UT ISI:000168915200008 ER PT J AU Derkowska, B Mulatier, JC Fuks, I Sahraoui, B Phu, XN Andraud, C TI Third-order optical nonlinearities in new octupolar molecules and their dipolar subunits SO JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS ID TETRATHIAFULVALENE DERIVATIVES AB The third nonlinear optical susceptibility (chi ((3))) of a new family of octupolar molecules with one, two, and three double bonds has been measured at 532 in tetrahydrofuran solutions by the degenerate four-wave mixing (DFWM) method. For comparison, we also measured the analogous dipolar subunits of the molecules. The second hyperpolarizability (gamma) for these molecules was deduced. We found that octupolar molecules exhibit large second-order hyperpolarizability (gamma) values. The gamma values obtained for octupolar compounds are approximately 10 times larger than those of their corresponding dipolar subunits and 10(4) times larger than those of CS2, which is a reference material for DFWM. (C) 2001 Optical Society of America. C1 Univ Angers, CNRS, UMR 6136, Lab Proprietes Opt Mat & Applicat, F-49045 Angers 01, France. Nicholas Copernicus Univ, Inst Phys, PL-87100 Torun, Poland. Ecole Normale Super Lyon, CNRS, Unite Mixte Rech, F-69364 Lyon, France. Czestochowa Tech Univ, Inst Phys, WSP, PL-4200 Czestochowa, Poland. RP Derkowska, B, Univ Angers, CNRS, UMR 6136, Lab Proprietes Opt Mat & Applicat, 2 Blvd Lavoisier, F-49045 Angers 01, France. TC 7 PD MAY PY 2001 VL 18 IS 5 BP 610 EP 616 UT ISI:000168548800004 ER PT J AU Verstraete, S Heudi, O Cailleux, A Allain, P TI Comparison of the reactivity of oxaliplatin, Pt(diaminocyclohexane)Cl-2 and Pt(diaminocyclohexane(1))(OH2)(2)(2+) with guanosine and L-methionine SO JOURNAL OF INORGANIC BIOCHEMISTRY DE oxaliplatin; Pt(dach)Cl-2 and Pt(dach)(OH2)(2+)(2); guanosine; methionine; reactivity; oxaliplatin metabolism ID CARCINOMA CELLS; CISPLATIN; PLATINUM; BINDING; CYTOTOXICITY; DNA; CIS-DIAMMINEDICHLOROPLATINUM(II); BIOTRANSFORMATION; METABOLITES; CARBOPLATIN AB The initial rates of reactivity of oxaliplatin, its metabolites Pt(dach)Cl-2 and Pt(dach)(OH2)(2)(2+) with guanosine and L-met in water, NaCl and phosphate were compared. Versus guanosine, the most reactive molecule was Pt(dach)(OH2)(2)(2+) about 40 fold that of oxaliplatin, the least reactive was Pt(dach)Cl-2. Versus L-met, Pt(dach)(OH2)(2)(2+), was also the most reactive species but only about 2 fold more reactive than Pt(dach)Cl-2 and oxaliplatin. Pt(dach)(OH2)(2)(2+) was approximately 3 fold less reactive versus methionine than guanosine whereas oxaliplatin and Pt(dach)Cl-2 were about seven fold more reactive versus methionine than guanosine. Thus, the three platinum compounds oxaliplatin, Pt(dach)Cl-2 and Pt(dach)(OH2)(2)(2+) react with L-met but only the Pt(dach)(OH2)(2)(2+) has a high reactivity with guanosine. Oxaliplatin, which is stable in water, has to be transformed in the presence of chloride in chloro-derivatives which are aquated to become active particularly versus guanosine. These data demonstrate that oxaliplatin has similarities with cisplatin in terms of chloride versus water coordination and in terms of dependence on chloride concentration for transformations. (C) 2001 Elsevier Science B.V. All rights reserved. C1 CHU Angers, Lab Pharmacol & Toxicol, F-49033 Angers 01, France. RP Allain, P, CHU Angers, Lab Pharmacol & Toxicol, 4 Rue Larrey, F-49033 Angers 01, France. TC 7 PD MAR PY 2001 VL 84 IS 1-2 BP 129 EP 135 UT ISI:000168175300016 ER PT J AU Sylvestre, F Beck-Eichler, B Duleba, W Debenay, JP TI Modern benthic diatom distribution in a hypersaline coastal lagoon: the Lagoa de Araruama (RJ), Brazil SO HYDROBIOLOGIA DE Brazil; coastal lagoon; hypersalinity; diatom; modern distribution; anthropic impact ID SALINITY AB Diatom assemblages identified in 75 surface sediment samples in a shallow hypersaline coastal lagoon (Lagoa de Araruama, R.J., Brazil) are mainly composed of holo-euryhaline and marine euryhaline benthic taxa. The lagoon is characterized by an assemblage dominated by Cocconeis placentula var. euglypta, associated with Catenula adhaerens and Cocconeis diminuta. However, different areas, characterized by specific assemblages, have been identified. Their distribution seems to be related to (1) fluctuations in bathymetry, influenced by water inputs from the ocean and from rivers adjacent to the lagoon; (2) fluctuations in salinity through the influence of marine water and the precipitation-evaporation rate. Moreover, the distribution of associations characterized by the presence of Nitzschia palea, N. pusilla and Fallacia cryptolyra suggests the influence of man-induced activities. Although these diatoms are not dominant, their wide distribution inside the lagoon, favored by local wind-hydrodynamics, suggest a large impact of anthropic activities (e.g. freshwater and organic sewages). C1 Univ Angers, UFR Sci, UPRESEA 2644, Geol Lab, F-49045 Angers, France. Univ Sao Paulo, Inst Oceanog, Dept Oceanog Fis, BR-05508900 Sao Paulo, Brazil. RP Sylvestre, F, Univ Angers, UFR Sci, UPRESEA 2644, Geol Lab, 2 Blvd Lavoisier, F-49045 Angers, France. TC 7 PD JAN PY 2001 VL 443 IS 1-3 BP 213 EP 231 UT ISI:000168065900019 ER PT J AU Cimon, B Symoens, F Zouhair, R Chabasse, D Nolard, N Defontaine, A Bouchara, JP TI Molecular epidemiology of airway colonisation by Aspergillus fumigatus in cystic fibrosis patients SO JOURNAL OF MEDICAL MICROBIOLOGY ID 3 TYPING METHODS; RESTRICTION ENDONUCLEASE ANALYSIS; INVASIVE PULMONARY ASPERGILLOSIS; POLYMORPHIC DNA; RANDOM AMPLIFICATION; GENETIC DIVERSITY; VIRULENCE; PATHOGENESIS; SEQUENCE; MARKERS AB A total of 109 sequential and multiple Aspergillus fumigatus isolates corresponding to 41 samples from seven cystic fibrosis (CF) patients was typed by random amplification of polymorphic DNA (RAPD) with the primer NS3 from the fungal ribosomal gene 18S subunit, and by sequence-specific DNA primer (SSDP) analysis. RAPD typing of the isolates revealed 10 different genotypes, whereas nine genotypes were identified by SSDP, Combination of the two typing methods permitted the differentiation of 25 overall genotypes, The colonisation typing patterns differed greatly between patients colonised for <1 year by A. fumigatus and long-term colonised patients. Two of three recently colonised patients presented a large number of types even in the same sample, unlike the chronically colonised patients, who harboured a limited number of genotypes, In the latter, the occurrence of a dominant genotype, usually the overall genotype 2, tended to reflect to the duration of colonisation. Moreover, anti-catalase antibodies to A. fumigatus appeared in most cases to be in response to genotype 2, These findings suggest that some strains of A, fumigatus may be selected during prolonged colonisation of the airways in CF patients. C1 Ctr Hosp Univ, Grp Etud Interact Hote Parasite, Lab Parasitol Mycol, Angers, France. Biotechnol Lab, UPRES 2161 Biocatalyse, Nantes, France. Sci Inst Publ Hlth Louis Pasteur, Sect Mycol, Brussels, Belgium. RP Cimon, B, Ctr Hosp Univ, Grp Etud Interact Hote Parasite, Lab Parasitol Mycol, Angers, France. TC 7 PD APR PY 2001 VL 50 IS 4 BP 367 EP 374 UT ISI:000167651500009 ER PT J AU Allain, P Etcharry-Bouyx, F Le Gall, D TI A case study of selective impairment of the central executive component of working memory after a focal frontal lobe damage SO BRAIN AND COGNITION ID SORTING TEST-PERFORMANCE; UTILIZATION BEHAVIOR; PREFRONTAL CORTEX; SCRIPTS; LESIONS; TASKS; REPRESENTATION; DEMENTIA; DISEASE; SYSTEM AB RC is a 36-year-old man who sustained a closed head injury with bilateral frontal lobe hypometabolism in 1978. In 1994, after a lobectomy of a large part of the left frontal lobe, he presented no behavioral disruption and normal performances on most of intelligence, longterm memory, and executive tests. However, he showed deficits in tasks that implicate short-term storage (i.e.. span tasks). These deficits in working memory were explored with regard to Baddeley's model using computerized tasks. On these tasks RC showed normal functioning of the articulatory loops and dysfunction of the central executive component in dual tasks. These results confirm those reported in another single case study by Van Der Linden, Coyette, and Seron (1993) and indicate that dual-task performance may assess one separable feature of executive functions. (C) 2001 Academic Press. C1 CHU Angers, Dept Neurol, Unite Neuropsychol, Psychol Lab, F-49033 Angers 01, France. Univ Angers, Psychol Lab, Angers, France. RP Allain, P, CHU Angers, Dept Neurol, Unite Neuropsychol, Psychol Lab, 4 Rue Larrey,EA 2646, F-49033 Angers 01, France. TC 7 PD FEB PY 2001 VL 45 IS 1 BP 21 EP 43 UT ISI:000167060300003 ER PT J AU Levadoux, E Morio, B Montaurier, C Puissant, V Boirie, Y Fellmann, N Picard, B Rousset, P Beaufrere, B Ritz, P TI Reduced whole-body fat oxidation in women and in the elderly SO INTERNATIONAL JOURNAL OF OBESITY DE fat oxidation; indirect calorimetry; aging; sex ID ENERGY-EXPENDITURE; OLDER WOMEN; EXERCISE; MEN; AGE; CARBOHYDRATE; INDIVIDUALS; METABOLISM; POTASSIUM; YOUNGER AB OBJECTIVE: To test the hypothesis that the increase in fat mass observed with aging might be related to a decrease in whole-body fat oxidation. SUBJECTS AND MEASUREMENTS: Forty volunteers had measurements of sleeping and 24 h substrate oxidation in calorimetric chambers, body composition with the O-18 dilution technique, VO2max, and fiber composition analysis from a biopsy of vastus lateralis. They were divided into 10 young women, 10 young men, 10 elderly women and 10 elderly men. RESULTS: Sleeping fat oxidation and 24 h fat oxidation were lower in women than in men and in elderly than in young participants. Sleeping fat oxidation was correlated to fat-free mass and energy balance (multivariate analysis). Twenty four hour fat oxidation was correlated to total energy expenditure and energy balance (multivariate analysis). After adjustment for differences in these factors, sleeping and 24 h fat oxidation were no longer different between age and sex groups. None of the parameters of macronutrient metabolism was correlated with muscle fiber composition. CONCLUSION: Our data suggest that fat oxidation is lower in elderly subjects. This difference could favour fat mass gain if fat intake is not adequately reduced. Differences in fat-free mass and in total energy expenditure appear to participate in the reduction in fat oxidation. C1 Human Nutr Lab, Clermont Ferrand, France. Physiol & Exercise Biol Lab, Clermont Ferrand, France. INRA, F-63122 St Genes Champanelle, France. Human Nutr Res Ctr, Auvergne, France. RP Ritz, P, CHU Angers, Serv Med B, F-49033 Angers 01, France. TC 7 PD JAN PY 2001 VL 25 IS 1 BP 39 EP 44 UT ISI:000166623700007 ER PT J AU Eliat, PA Lechaux, D Gervais, A Rioux-Leclerc, N Franconi, F Lemaire, L Dazord, L Catros-Quemener, V De Certaines, JD TI Is magnetic resonance imaging texture analysis a useful tool for cell therapy in vivo monitoring? SO ANTICANCER RESEARCH DE MRI; texture analysis; cell therapy; treatment follow-up ID EUROPEAN-ECONOMIC-COMMUNITY; CONCERTED RESEARCH-PROJECT; TISSUE CHARACTERIZATION; INTRACRANIAL TUMORS; MR; SPECTROSCOPY; PERMEABILITY; BRAIN AB Assessment of anti-tumor treatment efficiency is usually done by measuring tumor size. Treatment may however induce changes in the tumor other than tumor size. Magnetic Resonance Imaging Texture Analysis (MRI-TA) is presently used to follow activated lymphocyte cell therapy. We used a 7T micro-imager to acquire high-resolution MR images of an experimental liver metastasis from colon carcinoma in rats treated (n =4) or not (n =3) with a cell therapy product. MRI-TA was then performed with Linear Discriminant Analysis and showed: i) a significant variation of tumor texture with tumor growth and ii) a significant modification in the texture of tumors treated with activated lymphocytes compared with untreated tumors. T2-weighted images or volume calculation did not evidence any difference. MRI-TA appears as a promising method for early detection and follow-up of response to cell therapy. C1 ELIAT, LRMBM, F-35043 Rennes, France. Fac Med, Lab Biol Cellulaire, CNRS, URA 1529 GRETAC, F-35043 Rennes, France. UFR Sci, SCAS, F-49045 Angers, France. Fac Pharm Angers, SCAS, F-49100 Angers 01, France. Fac Pharm Angers, INSERM, ERIT M 0104, UPRES EA 2169, F-49100 Angers, France. RP Eliat, PA, ELIAT, LRMBM, CS 34317, F-35043 Rennes, France. TC 6 PD NOV-DEC PY 2001 VL 21 IS 6A BP 3857 EP 3860 UT ISI:000174447000019 ER PT J AU Heudi, O Brisset, H Cailleux, A Allain, P TI Chemical instability and methods for measurement of cisplatin adducts formed by interactions with cysteine and glutathione SO INTERNATIONAL JOURNAL OF CLINICAL PHARMACOLOGY AND THERAPEUTICS DE cisplatin; cisplatin aquated species; L-cysteine; glutathione; thiol-containing compounds reactivity; LC-UV-ICPMS; mass spectrometry ID ANTITUMOR AGENTS; DNA-BINDING; METHIONINE; CIS-DIAMMINEDICHLOROPLATINUM(II); NEPHROTOXICITY; OXALIPLATIN; METABOLITES; RESISTANCE AB Reactions between cisplatin or its aquated species and L-cysteine (L-cys) or glutathione (GSH) were studied in vitro using liquid chromatography on-line with inductively coupled plasma mass spectrometry (LC-ICPMS) and/or electrospray ionization mass spectrometry (LC-MS) in order to obtain information on the mechanisms occurring in treated patients. Reaction between cisplatin and L-cys yielded initially 4 adducts of which only 2 were stable and detectable after 24 hours incubation; their structures corresponded to bis-platinum cysteinyl adducts. Reaction of cisplatin with GSH proceeded via the formation of at least I I glutathione-platinum adducts (G1 - G11) which underwent parallel reactions within 24 hours of incubation, probably to form higher molecular weight species. Of the I I adducts, only 2, G3 and G7, whose structures correspond to [Pt(NH3)(2)Cl](2)(SG) and [Pt(NH3)(2)OH](2)(SG) were still present in the reaction mixture after 24 hours incubation. This study shows that GSH, and to a lesser extent L-cys, incubated with cisplatin in vitro forms unstable and reactive platinum compounds and that LC-ICPMS and LC-MS are 2 complementary techniques suitable for the study of organometallic compounds. C1 CHU Angers, Lab Pharmacol & Toxicol, F-49033 Angers 01, France. RP Allain, P, CHU Angers, Lab Pharmacol & Toxicol, 4,Rue Larrey, F-49033 Angers 01, France. TC 6 PD AUG PY 2001 VL 39 IS 8 BP 344 EP 349 UT ISI:000172232900004 ER PT J AU Hambli, R TI Finite element simulation of fine blanking processes using a pressure-dependent damage model SO JOURNAL OF MATERIALS PROCESSING TECHNOLOGY DE fine blanking; finite elements; damage law; fracture; experiment ID DUCTILE CRACK-GROWTH; VOID GROWTH; FRACTURE; RUPTURE AB In order to accurately simulate fine blanking processes, a finite element model valid for numerically describing of such operations has been developed. The numerical simulation of the damage evolution and crack initiation and propagation have been described by means of continuum damage approach. In this paper, two models for damage accumulation has been implemented in the finite element code ABAQUS by means of the user routine (UMAT). The Lemaitre damage model (referred to LMD model) taking into account the influence of triaxiality has been implemented as well as a damage model accumulation. based on the Rice and Tracey ductile fracture criterion allowing for the description of the exponential dependence on triaxiality of the stress field. The comparative study between the results obtained by the simulations using the different damage models and the experimental ones, showed that the LMD model is not able to predict the fracture propagation path in a realistic way. Only the exponential damage evolution gives good results. (C) 2001 Elsevier Science B.V. All rights reserved. C1 LASQUO, ISTIA, F-49000 Angers, France. RP Hambli, R, LASQUO, ISTIA, Upers JE 2039,62,Ave Notre Dame Lac, F-49000 Angers, France. TC 6 PD OCT 24 PY 2001 VL 116 IS 2-3 BP 252 EP 264 UT ISI:000171837700024 ER PT J AU Loiseau, J Vu, BL Macherel, MH Le Deunff, Y TI Seed lipoxygenases: occurrence and functions SO SEED SCIENCE RESEARCH DE fatty acid hydroperoxide; jasmonate; seed lipoxygenase; storage protein; stress resistance ID MEMBRANE-ASSOCIATED LIPOXYGENASE; FATTY-ACID HYDROPEROXIDES; JASMONIC ACID; METHYL JASMONATE; SOYBEAN COTYLEDONS; GERMINATING BARLEY; PLANT LIPOXYGENASE; MESSENGER-RNAS; ABSCISIC-ACID; PISUM-SATIVUM AB Lipoxygenases are widely distributed in the animal and plant kingdoms. These enzymes catalyse the hydroperoxidation of polyunsaturated fatty acids containing cis,cis-1,4-pentadiene moieties. Multiple isoenzymes, with different biochemical properties and tissue distribution, have been described for many plants. Lipoxygenases occur in vegetative tissues, but also accumulate in various seeds, and especially in leguminous seeds. Although several functions have been proposed for vegetative lipoxygenases, the roles of seed lipoxygenases remain enigmatic. In this review we discuss whether physiological functions assigned to vegetative lipoxygenases can be extended to seed isoforms. C1 UMP Physiol Mol Semences, F-49045 Angers 01, France. RP Macherel, MH, UMP Physiol Mol Semences, 16 Blvd Lavoisier, F-49045 Angers 01, France. TC 6 PD SEP PY 2001 VL 11 IS 3 BP 199 EP 211 UT ISI:000171559800002 ER PT J AU Le Gall-Recule, G Zwingelstein, F Yves, P Le Gall, G TI Immunocapture-RT-PCR assay for detection and molecular epidemiology studies of rabbit haemorrhagic disease and European Brown Hare Syndrome viruses SO JOURNAL OF VIROLOGICAL METHODS DE Rabbit Haemorrhagic Disease virus; European Brown Hare Syndrome virus immunocapture-RT-PCR; molecular epidemiology; viral diagnosis; sandwich-ELISA ID POLYMERASE CHAIN-REACTION; VIRAL HEMORRHAGIC-DISEASE; CAPSID PROTEIN GENE; HEPATITIS-A VIRUS; CALICIVIRUS; PURIFICATION; ANTIBODIES; CLONING; GENOME; FRANCE AB Rabbit Haemorrhagic Disease Virus and European Brown Hare Syndrome Virus are two members of the genus Lagovirus in the family Caliciviridae. They are the causative agents of highly contagious and fatal diseases of rabbits and hares respectively. We adjusted one assay for the detection and the genomic characterisation of each virus, based on viral purification by immunocapture and genomic amplification by reverse transcription-polymerase chain reaction (IC-RT-PCR). It is carried out directly with the liver exudate obtained after thawing and suppresses the viral nucleic acid preparation step. This assay combines the advantages of an ELISA test (rapidity) because immunocapture and the RT reaction were carried out in the same microtitre plate. and the advantages of PCR (sensitivity). The procedure described allows the processing of large numbers of samples and is suitable for phylogenetic studies of lagomorphs caliciviruses. In addition, it was compared with sandwich-ELISA used for Rabbit Haemorrhagic Disease or European Brown Hare Syndrome diagnosis. A good correlation was found between ELISA and IC-RT-PCR results for Rabbit Haemorrhagic Disease diagnosis, whereas for European Brown Hare Syndrome diagnosis, the results confirmed the higher sensitivity of the molecular method. (C) 2001 Elsevier Science B.V. All rights reserved. C1 AFSSA, Lab Etud & Rech Avicoles & Porcines, Unite Virol Immunol Parasitol Aviaires & Cunicole, F-22440 Ploufragan, France. Lab Departmental Vet Finistere, F-29334 Quimper, France. Lab Vet, Dept Maine & Loire, F-49009 Angers, France. RP Le Gall-Recule, G, AFSSA, Lab Etud & Rech Avicoles & Porcines, Unite Virol Immunol Parasitol Aviaires & Cunicole, Zoopole BP 53, F-22440 Ploufragan, France. TC 6 PD SEP PY 2001 VL 97 IS 1-2 BP 49 EP 57 UT ISI:000170677900005 ER PT J AU Cottenceau, B Hardouin, L Boimond, JL Ferrier, JL TI Model reference control for timed event graphs in dioids SO AUTOMATICA DE discrete-event systems; timed event graphs; dioid; residuation theory; feedback synthesis ID FEEDBACK; SYSTEMS AB This paper deals with feedback controller synthesis for timed event graphs in dioids. We discuss here the existence and the computation of a controller which leads to a closed-loop system whose behavior is as close as possible to the one of a given reference model and which delays as much as possible the input of tokens inside the (controlled) system. The synthesis presented here is mainly based on residuation theory results and some Kleene star properties. (C) 2001 Elsevier Science Ltd. All rights reserved. C1 Lab Ingn Syst Automatises, F-49000 Angers, France. RP Cottenceau, B, Lab Ingn Syst Automatises, 62 Av Notre Dame Lac, F-49000 Angers, France. TC 6 PD SEP PY 2001 VL 37 IS 9 BP 1451 EP 1458 UT ISI:000170272800015 ER PT J AU Guerin, V Lemaire, F Marfa, O Caceres, R Giuffrida, F TI Growth of Viburnum tinus in peat-based and peat-substitute growing media SO SCIENTIA HORTICULTURAE DE organic by-products; soilless crop; plant appearance; Viburnum tinus; substrate; peat ID WAXED CORRUGATED CARDBOARD; COMPOSTED GREEN WASTE AB To assess the growth of ornamental shrubs in peat alternative substrates, one ornamental species, Viburnum tinus L., was cultivated in a number of different substrates in two climates: a French oceanic (Oce) and a Spanish Mediterranean (Med). In Oce, three mixtures (1/1, v/v) of Finnish peat/yard compost, yard compost/raw coir and Finnish peat/raw coir were used, while the expanded perlite/composted manure (1/1), forest compost/composted bark (1/1) and forest compost/cattle manure compost (2/3) were tested in Med. A mixture (1/1) of Finnish peat/pine bark compost was used in both climates as a control. Plants were cultivated at a density of six plants/m(2) in 41 containers with drip irrigation. Plant height, dry mass, and leaf area were measured at intervals during cultivation. In Oce, substrates with yard compost or raw coir produced plants of similar size to those in the control substrate. In Med, forest compost/cattle manure compost mixture produced plants with the same height as those in the control mixture and the two other mixtures produced shorter plants. In both climates, substrates ranked the same whether height, dry mass or leaf area were considered. Using those parameters, significant effects of different substrates were revealed. Those effects are related to the substrate characteristics, mainly physical ones. As peat is used in a large range of situations, the experiment showed that substrate performance varies with its use, so alternative substrates can show better performance than those using peat. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, SAGAH UMR INRA INH, F-49071 Beaucouze, France. IRTA, Dept Tecnol Hort, Cabrils 08348, Spain. RP Guerin, V, Univ Angers, SAGAH UMR INRA INH, BP 57, F-49071 Beaucouze, France. TC 6 PD JUN 22 PY 2001 VL 89 IS 2 BP 129 EP 142 UT ISI:000169421900004 ER PT J AU Bernard, C Chaussedent, S Monteil, A Balu, N Obriot, J Duverger, C Ferrari, M Bouazaoui, M Kinowski, C Turrell, S TI Application of molecular dynamics techniques and luminescent probes to the study of glass structure: the SiO2-GeO2 case SO JOURNAL OF NON-CRYSTALLINE SOLIDS AB In this paper, we report on the results obtained from molecular dynamic simulation of a Eu3+-doped germanosilicate glass. This simulation provides further information on the structure. In particular it reveals a homogeneous distribution of SiO4 and GeO4 units, a decrease of defects compared to SiO2 and GeO2 glasses, and a trend to clustering of the doping ions. Using the modified crystal-field theory. the luminescence spectroscopic properties have been computed and comparison with experimental data has allowed a correlation of the spectral features with two main types of local environment depending on the coordination number and on the medium-range arrangement around the doping ions. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, Lab POMA, UMR CNRS 6136, F-49045 Angers, France. Univ Trent, Dipartimento Fis, INFM, I-38050 Trent, Italy. Ctr Fis Stati Aggregati, CNR, CeFSA, I-38050 Trent, Italy. USTL, CERLA, UMR CNRS 8523, Lab Phys Lasers Atomes & Mol,PhLAM, F-59655 Villeneuve Dascq, France. USTL, CERLA, UPR CNRS A2631L, LASIR,Lab Spectrochim Infrarouge & Raman, F-59655 Villeneuve Dascq, France. RP Bernard, C, Univ Angers, Lab POMA, UMR CNRS 6136, 2 Bd Lavoisier, F-49045 Angers, France. TC 6 PD MAY PY 2001 VL 284 IS 1-3 BP 68 EP 72 UT ISI:000169043800012 ER PT J AU Debenay, JP Geslin, E Eichler, BB Duleba, W Sylvestre, F Eichler, P TI Foraminiferal assemblages in a hypersaline lagoon, Araruama (RJ) Brazil SO JOURNAL OF FORAMINIFERAL RESEARCH ID BENTHIC FORAMINIFERA; POLLUTION; ENVIRONMENTS; AMMONIA AB Foraminiferal assemblages were studied in 93 samples collected in the lagoon of Araruama and in the adjacent area. The lagoon of Araruama is one of the largest hypersaline lagoons in the world, with a salinity range of 52-65 %o, Historical reports show that the lagoon has been hypersaline since at least the 16th century. Foraminiferal assemblages are dominated by miliolids, mainly Triloculina oblonga, and rotaliids, with Ammonia tepida and the less abundant Cribroelphidium excavatum var. selseyense, Textulariids are almost absent. This assemblage is similar to those usually reported from hypersaline lagoons with sandy carbonate-rich sediments and from salt marshes. A high proportion of aberrant tests was observed. Anthropogenic stresses do not seem to be responsible for these morphological abnormalities, which are attributed to high salinity conditions and to changes of salinity. However, the higher proportion of Ammonia tepida in the more impacted northern part of the lagoon is probably due to human impact. C1 Univ Angers, Geol Lab, F-49045 Angers, France. LEBIM, F-85350 Ile Dyeu, France. Univ Sao Paulo, Inst Oceanog, BR-05508900 Sao Paulo, SP, Brazil. RP Debenay, JP, Univ Angers, Geol Lab, EA 2644,2 Bd Lavoisier, F-49045 Angers, France. TC 6 PD APR PY 2001 VL 31 IS 2 BP 133 EP 151 UT ISI:000168841000006 ER PT J AU Niccoli-Sire, P Fayadat, L Siffroi-Fernandez, S Malthierry, Y Franc, JL TI Alternatively spliced form of human thyroperoxidase, TPOzanelli: Activity, intracellular trafficking, and role in hormonogenesis SO BIOCHEMISTRY ID HUMAN THYROID PEROXIDASE; ENDOPLASMIC-RETICULUM; DISEASE; IODINATION; ANTIGEN; IDENTIFICATION; PURIFICATION; LOCALIZATION; AUTOANTIGEN; MESSENGER AB Thyroperoxidase (TPO), a type I transmembrane heme containing glycoprotein, catalyzes iodide organification and thyroid hormone synthesis. One of the two main alternatively spliced forms of this enzyme, TPOzanelli, which is present in Graves's disease thyroid tissue, has a cytoplasmic domain completely modified. In the first stage of this study, the results of RT-PCR experiments showed that the TPOzanelli mRNA is present in normal thyroid tissue. We then generated CHO cell lines expressing the wild-type TPO (TPO1) and the alternatively spliced form TPOzanelli. Upon investigating a panel of 12 mAbs directed against the extracellular domain of TPO 1 and sera from patients with a high titer of TPO autoantibodies, we observed that (i) the three-dimensional structure of this domain is similar in both isoforms; (ii) the autoantibodies recognize TPOzanelli as well as TPO1. The results of pulse chase and cell surface biotinylation experiments showed that the TPOzanelli has a shorter half-life (7 versus 11 h) and is expressed at the cell surface in lesser amounts than TPO1 (7 versus 15%). The total enzymatic activity and cell surface activity were determined in CHO cells expressing TPO1 and TPOzanelli, and TPO1 and TPOzanelli were found to have similar levels of activity. It was established that approximately 20% of the TPO purified from a Graves' disease thyroid gland was precipitated by polyclonal antibodies directed against a specific part of the cytoplasmic tail of TPOzanelli. This confirmed that the protein corresponding to the mRNA is present in the thyroid tissue. All in all, these results indicate that TPOzanelli can be expected to play a role in thyroid hormone synthesis and in thyroid autoimmunity. C1 Univ Mediterranee, Fac Med, INSERM, U38, F-13385 Marseille 5, France. CHU Angers, Serv Biochim & Biol Mol, Angers, France. RP Franc, JL, Univ Mediterranee, Fac Med, INSERM, U38, 27 Bd J Moulin, F-13385 Marseille 5, France. TC 6 PD FEB 27 PY 2001 VL 40 IS 8 BP 2572 EP 2579 UT ISI:000167121500030 ER PT J AU Fortrat, JO Sigaudo, D Hughson, RL Maillet, A Yamamoto, Y Gharib, C TI Effect of prolonged head-down bed rest on complex cardiovascular dynamics SO AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL DE baroreflex; chaos; blood pressure regulation; heart rate variability; orthostatic hypotension ID RATE-VARIABILITY; FRACTAL NATURE; CHAOS THEORY; TIME-SERIES; SYSTEM; PRESSURE AB We postulated that a change in complex dynamics of the cardiovascular system could be involved in the orthostatic intolerance observed after simulated weightlessness. Supine recordings of 1024 consecutive pulse intervals and systolic blood pressures were obtained on 7 subjects adapted to a 42 day head-down bed rest (day 22 and 42) but also before and 6 days after head-down bed rest (-6 degrees). Coarse graining spectral analysis was used to extract the non-harmonic (fractal) component from each time series, The power spectral densities of this fractal component are inversely proportional to their frequency (l/f(beta)). We fitted an inverse power law estimate to the fractal component to determine the spectral exponent beta. The complex dynamics of blood pressure and heart rate variability were also analyzed by correlation dimension and non-linear prediction. Bed rest induced orthostatic intolerance in 4 subjects. There was a significant increase in the spectral exponent beta of RR-interval variability during and after head-down bed rest (before: 1.039+/-0.090; during: 1.552+/-0.080 and 1.547+/-0.100; after: 1.428+/-0.040). Analysis of the blood pressure dynamics indicated lower correlation dimensions during head-down bed rest and higher coefficients of predictability after head-down bed rest. Complexity alterations of RR-interval and blood pressure variability were not linked with one another during head-down bed rest. These alterations seemed to be correlated with the orthostatic intolerance observed after bed rest. These results suggest a change of the integration level of cardiovascular autonomic regulation. (C) 2001 Published by Elsevier Science B.V. C1 Fac Med Grange Blanche, Environm Physiol Lab, F-69373 Lyon 08, France. Univ Waterloo, Dept Kinesiol, Waterloo, ON N2L 3G1, Canada. Univ Tokyo, Fac Educ, Bunkyo Ku, Tokyo 113, Japan. RP Fortrat, JO, CHU Angers, Lab Explorat Fonct Vasc, F-49033 Angers, France. TC 6 PD JAN 14 PY 2001 VL 86 IS 3 BP 192 EP 201 UT ISI:000166599800005 ER PT J AU Tuech, JJ Regenet, N Hennekinne, S Pessaux, P Duplessis, R Arnaud, JP TI Impact of obesity on postoperative results of laparoscopic elective colectomy for sigmoid diverticulitis: prospective study. SO ANNALES DE CHIRURGIE DE colon; laparoscopic colectomy; obesity; sigmoid diverticulitis ID ASSISTED COLECTOMY; COLORECTAL SURGERY; NONOBESE PATIENTS; MORBIDLY OBESE; CHOLECYSTECTOMY; MULTICENTER; RESECTION; DISEASE; SERIES AB Study aim: The aim of this prospective study was to assess the outcome of laparoscopic colectomy for sigmoid diverticulitis in normal weighted, overweighted and obese patients. Patients and method: From January 1995 to December 2000, all patients (n=77) undergoing an elective colectomy for sigmoid diverticulitis were included in the study. The patients were divided into three groups: group 1 (n=29): normal weighted patients (BMI: 18-24.9); group 2 (n=27): overweighted patients (BMI: 25.0-29.9): group 3 (n=21): obese patients (BMI: 30.0-39.9). Comparison between these three groups was only made during the per and postoperative period. Results: There were no differences in the three groups with regard to age, sex and ASA classification. Duration of operation did not differ between group I and 2 (187 vs 210 min, P=0.6) but was shorter in group 1 than in group 3 (187 vs 247 min, P=0.003). Conversion rate did not differ and was respectively in group 1, 2 and 3: 13.8, 14.8 and 14.3%. The postoperative period during which parenteral analgesics were required was not different for group 1 and 2 but was longer in group 3 than in group 1 (8.5 vs 5.7 days, p=0.03). Morbidity rate was similar in group 1, 2 and 3: 15,14 and 17%. There was no perioperative mortality. Duration of hospital stay was similar in the three groups. Conclusion: Data from the present study suggest that laparoscopic colectomy for sigmoid diverticulitis can be applied safely to overweighted and obese patients. (C) 2001 Editions scientifiques et medicales Elsevier SAS. C1 CHU Angers, Dept Chirurg Digest, F-49000 Angers, France. RP Arnaud, JP, CHU Angers, Dept Chirurg Digest, 4 Rue Larrey, F-49000 Angers, France. TC 5 PD DEC PY 2001 VL 126 IS 10 BP 996 EP 1000 UT ISI:000172883300010 ER PT J AU Malchaire, JB Roquelaure, Y Cock, N Piette, A Vergracht, S Chiron, H TI Musculoskeletal complaints, functional capacity, personality and psychosocial factors SO INTERNATIONAL ARCHIVES OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH DE risk factors; neck; wrist-hand; ergonomics; stress ID CARPAL-TUNNEL SYNDROME; CUMULATIVE TRAUMA DISORDERS; LOW-BACK-PAIN; RISK-FACTORS; ASSEMBLY WORKERS; JOB DEMANDS; SYMPTOMS; INDUSTRY; EXPOSURE; WRIST AB Objectives: The aim of the research was to study the association between psychosocial and personality factors, and neck and wrist-hand musculoskeletal complaints, taking account of the occupational factors of force, posture and repetitiveness, and nonoccupational risk factors such as sport, hobbies, medical history. Methods: During personal interviews 133 women from seven different companies, working at constraining workplaces (very repetitive work), answered several questionnaires. These concerned: personal characteristics and history; work characteristics; psychosocial factors (perception and appreciation of the work situation, satisfaction at work, stress symptoms, Karasek questionnaire) and personality factors (neuroticism, conscientiousness, type-A behavior). They also undertook functional and psychomotor tests (wrist angles, grip strength and a dexterity test). Logistic regression models were calculated. Results: Wrist-hand complaints appear to be associated with some personal characteristics (smoking habits, fewer hobbies), work constraints (fewer breaks, heavy lifting efforts) and some personality (introversion) and psychosocial factors (worse appreciation of work). Neck complaints are also associated with some personal characteristics (young people, small, bad health, hormonal problems, fewer hobbies), some personality (urgency of time) and psychosocial factors (constraints as seen by the supervisor). Conclusion: The study confirms the multifactorial character of the musculoskeletal disorders and underlines the need for a global ergonomic approach to work situations, taking into account all their physical, psychological and social components. C1 Univ Catholique Louvain, Unite Hyg & Physiol Travail, B-1200 Brussels, Belgium. CHU, Ctr Consultat Pathol Professionnelle, Angers, France. RP Malchaire, JB, Univ Catholique Louvain, Unite Hyg & Physiol Travail, Clos Chapelle Champs 30-38, B-1200 Brussels, Belgium. TC 5 PD OCT PY 2001 VL 74 IS 8 BP 549 EP 557 UT ISI:000172520400004 ER PT J AU Diabira, S Rousselet, MC Gamelin, E Soulier, P Jadaud, E Menei, P TI PCV chemotherapy for oligodendroglioma: response analyzed on T2 Weighted-MRI SO JOURNAL OF NEURO-ONCOLOGY DE oligodendroglioma; chemotherapy; MRI ID AGGRESSIVE OLIGODENDROGLIOMA; RECURRENT OLIGODENDROGLIOMA; GLIOMAS; PROCARBAZINE; VINCRISTINE; CCNU; OLIGOASTROCYTOMAS; LOMUSTINE; DIAGNOSIS AB Objective: Because oligodendroglioma are infiltrative tumors, the Mac Donald's response criteria, usually used for solid and contrast-enhanced tumors, seem not to be adapted. To precise more relevant radiological criteria, the radio-logical response of oligodendroglioma to PCV chemotherapy was evaluated on T2 weighted-MRI sequences only. Methods: 25 patients with oligodendroglioma grade A or B were retrospectively analyzed. They were treated with up to six cycles of PCV standard regimen. Tumor size was calculated before and at the end of the treatment, on T2 weighted-MRI, by two methods: volumetric reconstruction (method 1) and maximal cross-sectional area (method 2). Responses were defined according to new criteria on T2 weighted-MRI. Results: According to these criteria and with the method 1, 7 of 25 patients (28%) had a partial response to the PCV, 14 patients (56%) had stabilized disease, and 4 patients (16%) had progressive disease. With the method 2, 6 had partial response (24%), 18 had stabilized disease (72%) and 1 had progressive disease. Conclusion: Response rate to PCV chemotherapy in this study was lower than response observed in the literature. Because of the infiltrative feature of oligodendroglioma, we think that the radiological response of these tumors should be evaluated on T2 weighted-MRI. The two methods of tumor size estimation used in this study were almost equivalent. Then, the maximal cross-sectional area measurement, more practical, could be retained. C1 Univ Hosp Angers, Dept Neurosurg, Angers, France. Univ Hosp Angers, Dept Pathol, Angers, France. Paul Papin Anticanc Ctr, Dept Neurooncol, Angers, France. RP Menei, P, CHU Angers, Serv Neurochirurg, 4 Rue Dominique Larrey, F-49000 Angers, France. TC 5 PD OCT PY 2001 VL 55 IS 1 BP 45 EP 50 UT ISI:000172383700006 ER PT J AU Canezin, J Cailleux, A Turcant, A Le Bouil, A Harry, P Allain, P TI Determination of LSD and its metabolites in human biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry SO JOURNAL OF CHROMATOGRAPHY B DE lysergic acid diethylamide ID LYSERGIC-ACID DIETHYLAMIDE; N-DEMETHYL-LSD; HUMAN URINE SPECIMENS; O-H-LSD; GAS-CHROMATOGRAPHY; MAJOR METABOLITE; 2-OXO-3-HYDROXY-LSD; EXTRACTION; SAMPLES; PLASMA AB A liquid chromatographic procedure with electrospray ionization tandem mass spectrometric detection has been developed and validated for LSD and iso-LSD determination. A one-step Equid-liquid extraction on I ml blood or urine was used. The lower limit for quantitative determination was 0.02 mug/l for LSD and iso-LSD. The analytical procedure has been applied in two positive cases (case 1: LSD=0.31 mug/l, iso-LSD=0.27 mug/l in plasma and LSD=1.30 mug/l, iso-LSD=0.82 mug/l in urine; case 2: LSD=0.24 mug/l, iso-LSD=0.6 mug/l in urine). LSD metabolism was investigated using MS-MS neutral loss monitoring for the screening of potential metabolites. The main metabolite was 2-oxo-3-hydroxy-LSD (O-H-LSD) present in urine at the concentrations of 2.5 mug/l and 6.6 mug/l, respectively, for case 1 and 2, and was not present in plasma. Nor-LSD was also found in urine at 0.15 and 0.01 mug/l levels. Nor-iso-LSD, lysergic acid ethylamide (LAE), trioxylated-LSD, lysergic acid ethyl-2-hydroxyethylamide (LEO) and 13 and 14-hydroxy-LSD and their glucuronide conjugates were detected in urine using specific MS-MS transitions. (C) 2001 Elsevier Science B.V. All rights reserved. C1 CHU Angers, Lab Pharmacol & Toxicol, F-49033 Angers 01, France. CHU Angers, Ctr Antipoison, F-49033 Angers, France. RP Cailleux, A, CHU Angers, Lab Pharmacol & Toxicol, 4 Rue Larrey, F-49033 Angers 01, France. TC 5 PD DEC 5 PY 2001 VL 765 IS 1 BP 15 EP 27 UT ISI:000172353700002 ER PT J AU Abadie, V Berthelot, J Feillet, F Maurin, N Mercier, A de Baulny, HO de Parscau, L TI Neonatal screening and long-term follow-up of phenylketonuria: the French database SO EARLY HUMAN DEVELOPMENT DE neonatal screening; phenylketonuria; French database ID EARLY-TREATED PHENYLKETONURIA; COST-BENEFIT; HYPOTHYROIDISM; DISCHARGE AB Background: In France, neonatal screening of phenylketonuria (PKU) started in 1966. A national association was created in 1978 in order to organise the neonatal screening program and to control the efficacy of the screening and patients' follow-up. Aims: To evaluate the results of the French PKU screening program in terms of hyperphenylalaninaemia epidemiology, efficacy of the screening procedure, management and outcome of the patients. Study design: The national database has been filled-up first with the answers to questionnaires that were sent each year by the PKU patients' physicians, and second with the results of an additional inquiry, which was set up in 1994 in order to investigate diagnosis, treatment, and school outcome of all French PKU patients. Results: PKU was diagnosed in 81.6% of patients with hyperphenylalaninaemia (HPA), non-PKU HPA in 17.2% and cofactor deficiency in 1.1%. From 1980, incidence of PKU has been stable: 1 per 17,124 live births. Sensitivity of the screening procedure was 99.3%. Age at diet initiation regularly decreased to reach 14 days as a median in 1996. Until 1990, median age at diet discontinuation was 6 years of age. Later, strict diet was continued longer (at lease up to 8-10 years). PKU patients who entered to secondary school at normal age were characterised by an earlier age at diagnosis and at diet initiation and a later age at diet discontinuation, compared to those who entered 1 year or more behind normal age. Conclusion: These data confirm the benefit of a nationwide organised screening program. They emphasise the importance of an early neonatal diagnosis and diet initiation in PKU patients and are consistent with the benefit of a longer period of strict diet in childhood. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved. C1 CHU Brest, Dept Pediat & Genet Med, F-29609 Brest, France. Hop Necker Enfants Malad, Serv Pediat Gen, Paris, France. CHU Angers, Serv Genet, Angers, France. Hop Enfants, Serv Reanimat Neonatale, Vandoeuvre Les Nancy, France. Hop Enfants La Timone, Serv Pediat Pluridisciplinaire, Marseille, France. Hop Robert Debre, Serv Neurometab, F-75019 Paris, France. RP de Parscau, L, CHU Brest, Dept Pediat & Genet Med, F-29609 Brest, France. TC 5 PD DEC PY 2001 VL 65 IS 2 BP 149 EP 158 UT ISI:000172115600007 ER PT J AU Pagano, A Joly, P Plenet, S Lehman, A Grolet, O TI Breeding habitat partitioning in the Rana esculenta complex: The intermediate niche hypothesis supported SO ECOSCIENCE DE hybrid zone; Rana esculenta; hybridogenesis; ecological niche; floodplains ID SPATIAL MOVEMENT PATTERNS; UPPER RHONE RIVER; HYBRID ZONES; WATER FROGS; TADPOLES; COMPETITION; FLOODPLAIN; HETEROSIS; DIVERSITY; LESSONAE AB The hypothesis of hybrids occupying intermediate niche was tested in the water frog hybridogenetic complex by investigating the variation of assemblage compositions in different habitats along a gradient of river influence in a large floodplain (upper Rhone, France). The parental species strongly differed in their habitat use. Whereas Rana ridibunda Pallas occupied dead arms close to the active channels, Rana lessonae Camerano occupied marsh ponds. The hybrid Rana kl. esculenta Linnaeus was found in the same ponds as Rana lessonae, as expected in L-E systems where Rana kl. esculenta females act as sexual parasites of Rana lessonae males. However, the proportions of each of these two taxa in mixed assemblages varied according to the degree of river influence, with Rana kl. esculenta predominating in ponds experiencing an intermediate level of flooding (alluvial marsh) and Rana lessonae predominating in less frequently flooded ponds (peat marsh). These results converge with other studies in demonstrating that the success of hybrid frogs depends on niche partitioning along an ecological gradient, which is probably a relevant templet for water frog evolution. In this evolutionary context, hybrid frogs have to deal with a trade-off between habitat selection and mate choice. C1 Univ Angers, F-49045 Angers, France. Univ Lyon 1, CNRS, UMR 5023, F-69622 Villeurbanne, France. Univ Geneva, LEBA, Geneva, Switzerland. Univ Lyon 1, CNRS, UMR 5023, F-69622 Villeurbanne, France. RP Pagano, A, Univ Angers, Campus Belle Beille, F-49045 Angers, France. TC 5 PY 2001 VL 8 IS 3 BP 294 EP 300 UT ISI:000171579500003 ER PT J AU Fournier, HD Menei, P Khalifa, R Mercier, P TI Ideal intraspinal implantation site for the repair of ventral root avulsion after brachial plexus injury in humans. A preliminary anatomical study SO SURGICAL AND RADIOLOGIC ANATOMY DE brachial plexus; avulsion; spinal cord; spinal nerve root ID SPINAL-CORD; NEURITE GROWTH; REGENERATION AB The advances made to date in root reimplantation for avulsion of the brachial plexus are modest considering that there are only reports from ten patients in the literature. However, the results are promising and should be applauded. The problem with reimplantation, in addition to the difficult surgical exposure, is to determine the ideal intraspinal implantation site for the graft. Given the non-permissive substrate properties of the CNS white matter, the outcome of grafts elsewhere in the spinal cord need to be evaluated. An inappropriate implantation site might explain the still modest recovery in this challenging group of patients. Consequently the microscopic anatomy of the cervical spinal cord segments C5 to T1 was studied. The aims were to 1- determine the relationships between the ventral gray horn and the ventrolateral sulcus of the spinal cord, and 2- analyse the location and course of the motoneuron fibres passing through the white matter to reach the ventral root. On the basis of the present findings it is proposed that reimplantation of nerve grafts or rootlets should be performed directly through the ventral root exit zone in contact with the ventromedial region of the ventral gray horn at a depth of 2 mm, rather than into the white matter of the lateral aspect of the cord. Initially we used a posterior approach with total facetectomies. An anterior approach is now evaluated for current use. C1 Fac Med, Lab Anat, F-49045 Angers, France. CHU Angers, Serv Neurochirurg, F-49033 Angers 01, France. RP Fournier, HD, Fac Med, Lab Anat, Rue Haute de Reculee, F-49045 Angers, France. TC 5 PD JUN PY 2001 VL 23 IS 3 BP 191 EP 195 UT ISI:000169765900009 ER PT J AU Hambli, R TI Blanking tool wear modeling using the finite element method SO INTERNATIONAL JOURNAL OF MACHINE TOOLS & MANUFACTURE DE blanking; finite element; tool wear; wear model; wear prediction AB One of the main objectives of the numerical process design in metal forming is to, develop adequate tool design and establish process parameter in order to increase tool life and to improve part quality and complexity while reducing manufacturing cost, The prediction of tool wear in sheet metal blanking/punching processes is investigated in this paper using the finite element method. A wear prediction model has been implemented in a finite element code in which the tool wear is a function of the normal pressure and some material parameters. A damage model is used in order to describe crack initiation and propagation into the sheet. The distribution of the tool wear on the tool profile is obtained and compared to industrial observations. Furthermore, a numerical investigation has been carried out to study the effect of tool wear on the bun: formation. (C) 2001 Elsevier Science Ltd. All rights reserved. C1 ISTIA, LASQUO, F-49000 Angers, France. RP Hambli, R, ISTIA, LASQUO, 62 Ave Notre Dame Lac, F-49000 Angers, France. TC 5 PD SEP PY 2001 VL 41 IS 12 BP 1815 EP 1829 UT ISI:000169780800009 ER PT J AU Hambli, R Potiron, A TI Comparison between 2D and 3D numerical modeling of superplastic forming processes SO COMPUTER METHODS IN APPLIED MECHANICS AND ENGINEERING DE superplastic forming; finite element; pressure-cycle prediction; experiment AB In this paper, the numerical results obtained by a finite element analysis in the case of superplastic sheet forming simulation are compared with the experimental ones to verify the validity of the finite element model (FEM) developed to predict the optimum pressure cycle, the deformed shapes, the distributions of the strain rate and the evolution of the thickness during the forming process. To compare the performance of 2D and 3D approaches, two analyses have been performed using a 2D model with 2D fully integrated continuum axisymmetric elements, and a 3D model with 3D fully integrated shell elements. Final results of the finite element modeling agree with the experimental ones. (C) 2001 Elsevier Science B.V. All rights reserved. C1 ENSAM, Lab Genie Mecan, F-49025 Angers, France. CAO, LAMCAO, F-49035 Angers, France. ISTIA, LASQUO, UPRES JE 2039, F-49000 Angers, France. RP Hambli, R, ENSAM, Lab Genie Mecan, 2 Blvd Ronceray,BP 3525, F-49025 Angers, France. TC 5 PY 2001 VL 190 IS 37-38 BP 4871 EP 4880 UT ISI:000169701800006 ER PT J AU Plucinski, KJ Kityk, IV Kasperczyk, J Sahraoui, B TI The structure and electronic properties of silicon oxynitride gate dielectrics SO SEMICONDUCTOR SCIENCE AND TECHNOLOGY ID 2ND-HARMONIC GENERATION AB Despite considerable progress achieved over the past few years in understanding ultrathin oxynitrides, several fundamental questions, in particular, the oxynitridation mechanism and the mechanisms behind the beneficial role of nitrogen are still not well understood. To improve understanding of the explanations which have been proposed for the phenomena specific to silicon oxynitride and for the nature of the defects, a study of the electron structure of a MOS system using silicon oxynitrides as the gate oxide and based on a molecular dynamics geometry optimization method, was carried out. Investigations of the band energy parameters versus the SiON film thickness and oxygen to nitrogen ratio were done theoretically as well as experimentally. Theoretical calculations were done by norm-conserving non-local pseudopotentials together with molecular dynamics geometry optimization. Experimental investigations included spectroscopic investigations of the film absorption. Both theoretical and experimental data indicate that the effective band energy gap possess modulated-like dependence versus the film thickness and oxygen/nitrogen ratio. The origin of the observed phenomenon is caused by specific electron-phonon anharmonic interactions between the film and the Si substrate. C1 Mil Univ Technol, PL-00908 Warsaw, Poland. Inst Phys, PL-42201 Czestochowa, Poland. Univ WSAZ Zawiercie, Zawiercie, Poland. Univ Angers, Lab POMA, Angers, France. RP Plucinski, KJ, Mil Univ Technol, 2 Kaliski Str, PL-00908 Warsaw, Poland. TC 5 PD JUN PY 2001 VL 16 IS 6 BP 467 EP 470 UT ISI:000169258300010 ER PT J AU Hamama, L Baaziz, M Letouze, R TI Somatic embryogenesis and plant regeneration from leaf tissue of jojoba SO PLANT CELL TISSUE AND ORGAN CULTURE DE plant regeneration; Simmondsia chinensis; somatic embryogenesis ID INVITRO PRODUCTION; GROWTH AB A protocol was developed for the induction, maturation and germination of somatic embryos from leaf tissue of jojoba [Simmondsia chinensis (Link) Schneider]. Explants were placed on their adaxial sides in Petri dishes and maintained in darkness on half-strength Murashige and Skoog basal medium (MS/2). Combinations of 2,4-dichlorophenoxyacetic acid (1.35-4.52 muM) with 6-benzylaminopurine (1.33-4.43 muM) and 2 synthetic cytokinins, N-(2-chloro-4pyridyl)-N'-phenylurea (1.21-4.03 muM) or (E)-6-[3-(trifluoromethyl)-but-2-enylamino] purine (1.11-3.71 muM) resulted in formation of embryogenic cultures and somatic embryos. After two 30-day subcultures, embryogenic cultures were transferred onto MS/2 medium supplemented with different auxins and cytokinins. Somatic embryo maturation, germination and plantlet formation were achieved using 1-naphthaleneacetic acid (3.75 muM) or indole-3-butyric acid (3.44 muM) in combination with BA (0.44 or 1.33 muM) or F3iP (0.37 or 1.11 muM). Histology confirmed each stage of development. C1 Lab Rech Physiol Vegetale Pays Loire, F-49045 Angers 01, France. Lab Biochim & Ameliorat Plantes BAP, Fac Sci Semlalia, Dept Biol, Marrakech 40000, Morocco. RP Letouze, R, Lab Rech Physiol Vegetale Pays Loire, 16 Blvd Lavoisier, F-49045 Angers 01, France. TC 5 PY 2001 VL 65 IS 2 BP 109 EP 113 UT ISI:000169218800002 ER PT J AU Hunault-Berger, M Milpied, N Bernard, M Jouet, JP Delain, M Desablens, B Sadoun, A Guilhot, F Casassus, P Ifrah, N TI Daunorubicin continuous infusion induces more toxicity than bolus infusion in acute lymphoblastic leukemia induction regimen: a randomized study SO LEUKEMIA DE acute lymphoblastic leukemia; daunorubicin; continuous infusion; toxicity ID ACUTE LYMPHOCYTIC-LEUKEMIA; NEWLY-DIAGNOSED ADULT; YORK-II PROTOCOL; VAD REGIMEN; CANCER; VINCRISTINE; THERAPY; RISK; CHEMOTHERAPY; DOXORUBICIN AB We report the first randomized study assessing the efficacy and safety of daunorubicin (DNR) continuous infusion (CI) compared to the more conventional 30 min infusion (i.v.) in newly diagnosed adult acute lymphoblastic leukemia (ALL). Seventy-seven patients were initially randomized to receive either a 24h CI DNR (60 mg/m(2) days 2-4) (40 patients) or bolus DNR at the same dosage (37 patients) with vincristine (2 mg i.v. days 1, 8, 15) and oral prednisone (60 mg/m(2) days 1-15), without hematopoietic growth factor support, as an induction regimen. The distribution of adverse prognostic factors was comparable in the two-induction arm. Acute toxicity was more important in the CI arm. Gram negative infection (9 vs 1 gram negative septicemia, P = 0.01) and infection-related deaths (6 vs 1 deaths, P = NS) occurred more frequently in the CI arm during the induction treatment than in the i.v. arm, leading to the study interruption. Neutropenia but not thrombopenia duration was significantly longer in the CI arm than in the i.v. arm (18 days vs 14 days, P > 0.05 and 16 days vs 12 days, P > 0.05, respectively). Despite a similar CR rate according to the method of DNR administration (68% in the CI DNR arm vs 76% in the i.v. arm after the first course), there was a trend toward higher freedom from relapse (FFR) after DNR CI (48% vs 28% in the i.v. arm at 5 years, P = NS), suggesting that despite this high toxicity, DNR CI may improve the CR quality and decrease further the residual disease. C1 Univ Hosp Angers, Div Hematol, Angers, France. Univ Hosp Nantes, Div Hematol, Nantes, France. Univ Hosp Rennes, Div Hematol, Rennes, France. Univ Hosp Lille, Div Hematol, Lille, France. Univ Hosp Tours, Div Hematol, Tours, France. Univ Hosp Amiens, Div Hematol, Amiens, France. Ctr Hosp Reg & Univ Poitiers, Div Hematol, F-86021 Poitiers, France. Univ Hosp Bobigny, Div Hematol, Bobigny, France. RP Ifrah, N, CHU Angers, F-49033 Angers, France. TC 5 PD JUN PY 2001 VL 15 IS 6 BP 898 EP 902 UT ISI:000169214700004 ER PT J AU Chabasse, D TI Interest of yeast numeration in urine. Review of literature and preliminary results of a multicentric prospective study carried out in 15 hospital centers. SO ANNALES FRANCAISES D ANESTHESIE ET DE REANIMATION DE candidiasis; candiduria; intensive care unit; prognosis; prospective study; risk factors ID ILL SURGICAL PATIENTS; TRACT INFECTIONS; AMPHOTERICIN-B; CANDIDURIA; FUNGURIA; FLUCONAZOLE; MANAGEMENT; FREQUENCY; SURVEILLANCE; CANDIDIASIS AB Candida spp. are increasingly involved in nosocomial infections in severely ill patients and the diagnosis is difficult. In this context, the significance of candiduria remains unclear. Management of this condition is still equivocal, because of the lack of information about its natural history and its predictive value for disseminated infection. Little is known about the discriminant value of colony count. After a comprehensive review of the available published data, the preliminary results of a multicentric prospective survey in critically ill patients hospitalised in intensive care units are given. The aim of the study was to search for correlations between quantitative candiduria and known risk factors for disseminated candidiasis. There is a statistically significant correlation (p = 0.003) between heavy candiduria (> 10(4) cfu . ml(-1)) and high Pittet colonisation index (greater than or equal to 0.5). Quantification of candiduria could be useful to select patients at high risk for disseminated candidiasis. (C) 2001 Editions scientifiques et medicales Elsevier SAS. C1 CHU Angers, Lab Parasitol Mycol, F-49033 Angers 01, France. RP Chabasse, D, CHU Angers, Lab Parasitol Mycol, 4 Rue Larrey, F-49033 Angers 01, France. TC 5 PD APR PY 2001 VL 20 IS 4 BP 400 EP 406 UT ISI:000168734500013 ER PT J AU Le Guillou, H Le Meur, A Bourdon, S Loison, J Fialaire, P Chennebault, JM Kouyoumdjian, S Payan, C TI Antibody avidity: use for the diagnosis of HIV early infection SO ANNALES DE BIOLOGIE CLINIQUE DE avidity; maturation; automate; HIV1 ID PREGNANT-WOMEN; IGG AB Determination of IgG avidity is useful to distinguish primary infection from reactivation or reinfection in viral, parasitic or bacterial infections. For diagnosis of HIV type 1 primary infection, the detection of IgM antibodies is often useless since they are also found in chronic infection. The usual serology (Elisa, western-blot. p24 antigen) may present no interest if done too late (more than 2 or 3 months after infection. Therefore. we have developed a test to determine the avidity of anti-HIV 1 antibodies, using 1 M guanidine as denaturing agent. We have adapted the measurement of avidity to the Axsym automatic system for a routine use. indeed, since requests for avidity determinations are sporadic, the use of microplates is not convenient. Using this assay. we found a low avidity (less than 50%) in immunocompetent and recent infected patients (less than 6 months), compared to old infected patients (more than 12 months) who had high avidity (80 to 100%). However, early treated patients (in the 6 months after contamination) had also low avidities hut with a slower development of antibody maturation (8 to 27 months versus 2 to 8 months in non treated patients). To conclude, the determination of the anti-HIV 1 avidity, according to the proper procedures explained here (notion of treatment and/or serious immunodepression), may help the physician to date the infection in each new infected patient who might benefit from an early treatment. C1 CHU Angers, Lab Bacteriol Virol, F-49033 Angers, France. CHU Angers, Serv Malad Infect, F-49033 Angers, France. RP Payan, C, CHU Angers, Lab Bacteriol Virol, 4 Rue Larrey, F-49033 Angers, France. TC 5 PD JAN-FEB PY 2001 VL 59 IS 1 BP 41 EP 47 UT ISI:000168492400008 ER PT J AU Kityk, IV Fahmi, A Sahraoui, B Rivoire, G Feeks, I TI Nitrobenzene as a material for the fast-respond degenerate four-wave mixing SO OPTICAL MATERIALS DE nitrobenzene; DFWM; electron-vibration interactions ID LIQUID WATER; SB2SE3-BACL2-PBCL2 GLASSES AB We have revealed a possibility of using nitrobenzene (NB) as a promising for time-resolving degenerate four-wave mixing (DFWM). The DFWM efficiency versus time delay between the probe and pump beams was revealed. The four time-retarded maxima of the DFWM are caused by specific electron-vibration interactions in the NB. An essential contribution of acoustical vibrations is demonstrated. Molecular dynamics geometry optimisation of the NB molecule is done taking into account intermolecular interactions. Superposition of all possible molecular conformations with appropriate weighting factors and higher-order intermolecular multiple interactions is considered. The observed DFWM behavior is explained within the framework of ab inito quantum chemical calculations with inclusion of excited configuration interaction (CI) states. We considered both harmonic as well anharmonic electron-vibration interactions. The average lifetime of MO levels contributing to the third-order nonlinear and time kinetics of the observed DFWM dependences is estimated. Advantages and drawbacks of the prosposed method are discussed. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, UFR Sci, Lab POMA, CNRS,EP 130, F-49045 Angers, France. Univ Maine, Fac Sci, Lab Phys Etat Condense 1, F-72085 Le Mans 09, France. RP Kityk, IV, Solid State Dept Czestochowa WSP, PL-42217 Czestochowa, Poland. TC 5 PD MAY PY 2001 VL 16 IS 4 BP 417 EP 429 UT ISI:000168237100002 ER PT J AU Lode, T TI Character convergence in advertisement call and mate choice in two genetically distinct water frog hybridogenetic lineages (Rana kl esculenta, Rana kl grafi) SO JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH DE allozymic variation; convergence; hybrid zones; hybridogenesis; klepton; mating call; water frogs ID MATING-CALL; TERRITORIAL CALLS; HYBRID; EXCLUSION; RIDIBUNDA; RANIDAE; HYLIDAE; HYBRIDIZATION; COMPATIBILITY; DISPLACEMENT AB Patterns of advertisement call were investigated in two genetically distinct water frog lineages (Rana ici esculenta, Rana. kl grafi), which were identified by starch gel electrophoresis - the aim being to determinate the role of vocalization in the hybridogenetic process. Both hybrids displayed major modifications from the basic structure of the Rana ridibunda call. In Rana kl grafi, the call structure tended to correspond to that of Rana perezi in most of the studied parameters (frequency, duration, number of pulses) whereas the call of Rana kl esculenta tended to resemble that of Ra,la lessonae. The ascendant hierarchical classification clearly revealed such a convergence toward parental species and accounted for a divergence between hybrids. Changes in call patterns might result from both the expression of the non-ridibunda genome and the sexual selective pressure through female mate choice. Only few non-ridibunda females exhibited a preference for hybrid calls which, however, allowed some hybridogenetic males to obtain successful mates. Thus, hybridogenesis induced character convergence in courtship signal with the non-ridibunda species in hybrid zones. In any case, these changes in the courtship signal favoured the particular hybridogenetic process, which constitutes a quasi-parasitism of the non-ridibunda genome. C1 Univ Angers Belle Beille, Fac Sci, Lab Ecol Anim, F-49045 Angers, France. RP Lode, T, Univ Angers Belle Beille, Fac Sci, Lab Ecol Anim, 2 Bd Lavoisier, F-49045 Angers, France. TC 5 PD MAR PY 2001 VL 39 IS 1-2 BP 91 EP 96 UT ISI:000168090000009 ER PT J AU Bremond, A Bataillard, A Thomas, L Achard, JL Fervers, B Fondrinier, E Lansac, J Bailly, C Hoffstetter, S Basuyau, JP d'Anjou, J Descamps, P Farsi, F Guastalla, JP Laffargue, F Rodier, JF Vincent, P Pigneux, J CA Grp Travail TI Standards, Options and Recommendations for the surgical management of carcinoma of the endometrium SO BULLETIN DU CANCER DE carcinoma of the endometrium; surgery; recommendations for clinical practice; cancer; adult ID CLINICAL STAGE-I; PAPILLARY SEROUS CARCINOMA; LYMPH-NODE METASTASES; PARAAORTIC LYMPHADENECTOMY; RADIATION-THERAPY; PROGNOSTIC FACTORS; GYNECOLOGIC MALIGNANCIES; VAGINAL HYSTERECTOMY; HIGH-RISK; ABDOMINAL HYSTERECTOMY AB Context. The "Standards, Options and Recommendations" (SOR) project, started in 1993, is a collaboration between the Federation of the French Cancer Centres (FNCLCC), the 20 French Cancer Centres and specialists from French Public Universities, General Hospitals and Private Clinics. The main objective is the development of clinical practice guidelines to improve the quality of health care and outcome for cancer patients. The methodology is based on literature review and critical appraisal by a multidisciplinary group of experts with feedback from specialists in cancer care delivery. Objectives. To develop clinical practice guidelines according to the def;definitions of the Standard, Options and Recommendations project for the surgical management of carcinoma of the endometrium. Methods. Data were identified by searching Medline (R) and personal reference lists of members of the expert groups. Once the guidelines were defined, the document was submitted for review to independent reviewers, and to the medical committees of the 20 French Cancer Centres. Results. The main recommendations for the surgical management of carcinoma of the endometrium ave: 1) where-ever possible, surgery is the primary treatment of both localised and advanced disease: 2) surgery is performed according to the stage of the cancer and the status of the patient; 3) surgery for stages I and II disease entails total abdominal hysterectomy and bilateral salpingo-oophorectomy. A modified radical hysterectomy is undertaken in cases of macroscopic cervical involvement. An omenectomy is recommended for serous papillary types. Pelvic lymphadenectomy for the purposes of precise staging is undertaken if the patient is of good performance status and without bad pronostic factors. Para-aortic lymphadenectomy ran be undertaken to determine involvement of para-aortic nodes; 4) surgery for stages III and IV: radical surgery must be undertaken if at all possible with additional treatment as indicated In the case of advanced disease, debulking surgery is indicaded. C1 Ctr Reg Leon Berard, Lyon, France. Clin St Catherine, Avignon, France. Ctr Paul Strauss, Strasbourg, France. CHU Montpellier, Hop Arnaud de Villeneuve, Montpellier, France. CHU Angers, Angers, France. Ctr Henri Becquerel, F-76038 Rouen, France. Ctr Alexis Vautrin, Nancy, France. CHU Bretonneau, F-37044 Tours, France. Ctr Paul Papin, Angers, France. Ctr Jean Perrin, Clermont Ferrand, France. Inst Bergonie, Bordeaux, France. FNCLCC, Paris, France. RP Bremond, A, Ctr Reg Leon Berard, Lyon, France. TC 5 PD FEB PY 2001 VL 88 IS 2 BP 181 EP 198 UT ISI:000167681400006 ER PT J AU Buldyreva, J Benec'h, S Chrysos, M TI Oxygen-broadened and air-broadened linewidths for the NO infrared absorption bands by means of the exact-trajectory approach SO PHYSICAL REVIEW A ID NITRIC-OXIDE; LINE PARAMETERS; COEFFICIENTS; RANGE AB Semiclassical ab initio values of oxygen-broadened and air-broadened NO infrared linewidths are reported in the range of atmospheric temperatures 163-299 K. The calculation is based on the authors' recently developed extended exact trajectory approach implemented within the Robert-Bonamy formalism for collisional line broadening. The results obtained are found to be in excellent agreement with recent straightforward measurements. C1 Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, UMR 6136, F-49045 Angers, France. RP Buldyreva, J, Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, UMR 6136, 2 Blvd Lavoisier, F-49045 Angers, France. EM jeanna.buldyreva@univ-angers.fr TC 5 PD MAR PY 2001 VL 6303 IS 3 AR 032705 DI ARTN 032705 UT ISI:000167321000061 ER PT J AU Moreno-Manas, M Pleixats, R Andreu, R Garin, J Orduna, J Villacampa, B Levillain, E Salle, M TI The first 1,3-dithiol-2-ylidene donor-pi-acceptor chromophores containing an azine spacer: synthesis, electrochemical and nonlinear optical properties SO JOURNAL OF MATERIALS CHEMISTRY ID PUSH-PULL POLYENES; CRYSTAL-STRUCTURES; EFFICIENT IMPLEMENTATION; SEMIEMPIRICAL METHODS; ELECTRONIC-STRUCTURE; ACETOPHENONE AZINES; EXCITATION-ENERGIES; CHEMICAL-SHIFTS; 2ND-ORDER; MOLECULES AB The first push-pull 1,3-dithiol-2-ylidene derivatives containing an azine spacer have been prepared in order to evaluate the effect of the azine bridge on the second-order NLO properties of these compounds, which show mu beta (0) values lower than those of similar derivatives endowed with ethylenic spacers. The linear and nonlinear optical properties of these compounds have been studied both experimentally and theoretically by TD-DFT and ab initio CPHF calculations respectively. Moreover, the E-Z photoisomerization of one of these azines is reported. C1 Univ Autonoma Barcelona, Dept Quim, E-08193 Barcelona, Spain. Univ Zaragoza, CSIC, Dept Quim Organ ICMA, E-50009 Zaragoza, Spain. Univ Zaragoza, CSIC, Dept Fis Mat Condensada ICMA, E-50009 Zaragoza, Spain. Univ Angers, Lab Ingn Mol & Mat Organ, CNRS, UMR 6501, F-49045 Angers, France. RP Moreno-Manas, M, Univ Autonoma Barcelona, Dept Quim, E-08193 Barcelona, Spain. TC 5 PY 2001 VL 11 IS 2 BP 374 EP 380 UT ISI:000167139700026 ER PT J AU Boury, F Jean-Claude, G Bouligand, Y Proust, JE TI Interfacial properties of amiodarone: the stabilizing effect of phosphate anions SO COLLOIDS AND SURFACES B-BIOINTERFACES DE arrhytmias; surface pressure; liquid crystal; bilayers; charged monolayers AB Amiodarone, a drug used in heart therapy, is poorly soluble in water at room temperature, but forms transparent phases much more concentrated than the critical micellar concentration (CMC), when crystals are heated (above 60 degreesC) in presence of water and cooled down to room temperature. These pseudosolutions were supposed to be made of a complex system of micelles. In order to better understand the effects of pH and ion species on the supramolecular organization of amiodarone, interfacial pressure measurements were performed at the air;water interface on a Langmuir trough. Monolayers spread from chloroformic solutions over non bufferered subphases were insoluble at basic pH (NaOH, pH 10) but soluble at acidic pH (HCl, pH 4). However, a higher ionic strength obtained by adding NaCl (0.15 N) or NaH2PO4 (0.15 N) to the subphase stopped the amiodarone solubilization. On an acidic phosphate subphase (NaH2PO4, pH 4.4, 0.15 N), abnormally high surface pressures (> 1 mN/m) were measured for high molecular areas (80-200 Angstrom (2)/molecule) suggesting a supramolecular organization of the surface film. Insoluble monolayers were also obtained when the amiodarone supramolecular pseudosolution was spread on neutral (NaH2PO4, pH 6.25, 0.15 N) or acidic (NaH2PO4, pH 4.4, 0.15 N) subphases. However, a great instability on basic subphase (phosphate buffer pH 8.8) indicated the breakage of the supramolecular structure during spreading. These results are discussed taking into account the amiodarone state of ionization and the electrostatic interactions with counterions. Combining the use of phosphate counterions and that of acidic pH opens new perspectives in the optimization of amiodarone intravenous formulations. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Fac Pharm Angers, Unite Vectorisat Particulaire, UPRES EA 2169, F-49100 Angers, France. Sanofi Rech, Ctr Montpellier, F-34184 Montpellier, France. Ecole Prat Hautes Etud, F-49100 Angers, France. TC 5 PD MAR PY 2001 VL 20 IS 3 BP 219 EP 227 UT ISI:000166763300003 ER PT J AU Parusinski, A Pragacz, P TI Characteristic classes of hypersurfaces and characteristic cycles SO JOURNAL OF ALGEBRAIC GEOMETRY ID CHERN CLASSES; VARIETIES AB We give a new formula for the Chern-Schwartz-MacPherson class of a hypersurface with arbitrary singularities, generalizing the main result of [P-P), which was a formula for the Euler characteristic. Two different approaches are presented. The first is based on the theory of characteristic cycles of a D-module (or a holonomic system) and the work of Sabbah [S], Briancn-Maisonobe-Merle [B-M-M], and Le-Mebkhout [L-M]. In particular, this approach leads to a simple proof of a formula of Aluffi [A] for the above mentioned class. The second approach uses Verdier's [V] specialization property of the Chern-Schwartz-MacPherson classes. Some related new formulas for complexes of nearby cycles and vanishing cycles are also given. C1 Univ Angers, Dept Math, F-49045 Angers 01, France. Polish Acad Sci, Inst Math, PL-00950 Warsaw, Poland. RP Parusinski, A, Univ Angers, Dept Math, 2 Bd Lavoisier, F-49045 Angers 01, France. TC 5 PD JAN PY 2001 VL 10 IS 1 BP 63 EP 79 UT ISI:000165568200004 ER PT J AU Legrand, E Flipo, RM Guggenbuhl, P Masson, C Maillefert, JF Soubrier, M Saraux, A Di Fazano, CS Sibilia, J Goupille, P Chevalier, X Cantagrel, A Conrozier, T Ravaud, P Liote, F CA Rheumatology Network Org TI Management of nontuberculous infectious discitis. Treatments used in 110 patients admitted to 12 teaching hospitals in France SO JOINT BONE SPINE DE antibiotics; diagnosis; pyogenic discitis; treatment ID SPONDYLITIS AB The optimal management of pyogenic discitis is not agreed on. No randomized clinical trials of short-course or oral antibiotic regimens have been published to date, To shed light on this issue, we reviewed the management of patients admitted for pyogenic discitis to one of 12 networked rheumatology departments. In this cross-sectional observatonal study, each department included the first ten patients admitted starting in January 1997 for treatment of pyogenic discitis. One hundred ten patients met the inclusion criteria, 67 men and 43 women, with a mean age of 60.6 +/- 13.7 years (range, 17-86 years), Mean time from symptom onset to diagnosis was 39.6 +/- 39.8 days (range, 24 h-240 days), Blood cultures were positive in 47.3% of patients, and the percutaneous discal and vertebral biopsy in 63.6% of cases; these two investigations identified the causative organism in 79 cases (72.8%). Mean duration of the rheumatology department stay was 31.3 +/- 14.1 days (range, 4-78 days). Antibiotics were given intravenously to 103 (93.6%) patients, for a mean of 25.5 +/- 17.6 days (range, 4-124 days); duration of intravenous antibiotic therapy was longer than 4 weeks in 36,5% of patients, Only seven (6.4%) patients received primary oral antibiotics with no parenteral antibiotics. One hundred patients were given oral antibiotics at the same time as and after intravenous antibiotics, for a mean duration of 87.2 +/- 43.6 days (range, 20-278 days); Bracing was used in 98 (89.1 %) patients. Although antibiotic selection was rational and in agreement with current recommendations, wide differences were noted across centers regarding intravenous treatment duration, hospital stay duration, and total treatment duration. (C) 2001 Editions scientifiques et medicales Elsevier SAS. C1 Teaching Hosp, Dept Rheumatol, Angers, France. Teaching Hosp, Dept Rheumatol, Lille, France. Teaching Hosp, Dept Rheumatol, Rennes, France. Teaching Hosp, Dept Rheumatol, Dijon, France. Teaching Hosp, Dept Rheumatol, Clermont Ferrand, France. Teaching Hosp, Dept Rheumatol, Lyon, France. Teaching Hosp, Dept Rheumatol, Brest, France. Teaching Hosp, Dept Rheumatol, Limoges, France. Teaching Hosp, Dept Rheumatol, Strasbourg, France. Teaching Hosp, Dept Rheumatol, Tours, France. Teaching Hosp, Dept Rheumatol, Paris, France. Teaching Hosp, Dept Rheumatol, Toulouse, France. RP Legrand, E, CHU Angers, Serv Rhumatol, F-49033 Angers, France. TC 4 PD DEC PY 2001 VL 68 IS 6 BP 504 EP 509 UT ISI:000173094900011 ER PT J AU Mercier, N Giffard, M Pilet, G Allain, M Hudhomme, P Mabon, G Levillain, E Gorgues, A Riou, A TI (TTF)(2)[TTF(CO2H)(2)(CO2)(2)]: a wholly TTF material containing TTF radical cations and TTF derived anions SO CHEMICAL COMMUNICATIONS ID BEDT-TTF; CRYSTAL; SALTS AB Electrooxidation of tetrathiafulvalene (TTF) carried out in the presence of (Bu4N)(2)TTF(CO2H)(2)(CO2)(2) as supporting electrolyte affords wholly TTF organic materials in which TTF cations are associated with TTF(CO2H)(2)(CO2-)(2) as counteranions. C1 CNRS, UMR 6501, F-49045 Angers, France. RP Mercier, N, CNRS, UMR 6501, 2 Bd Lavoisier, F-49045 Angers, France. TC 4 PD DEC 21 PY 2001 IS 24 BP 2722 EP 2723 UT ISI:000173044900098 ER PT J AU Colombat, P Foussard, C Bertrand, P Cornillet-Lefebvre, P Milpied, N Escoffre-Barbe, M Maisonneuve, H Pignon, B Gressin, R Delwail, V Ramee, JF Travade, P Delepine, R Deconinck, E TI Value of autologous stem cell transplantation in first line therapy of follicular lymphoma with high tumor burden: First results of the randomized GOELAMS 064 trial. SO BLOOD C1 Hop Bretonneau, Tours, France. CHU Angers, Angers, France. Hop Debre, Reims, France. Hop Hotel Dieu, Nantes, France. Hop Morvan, Brest, France. CHD, La Roche Sur Yon, France. Hop Michallon, Grenoble, France. Hop Jean Bernard, Poitiers, France. Ctr Sienne, Nantes, France. Hop Hotel Dieu, Clermont Ferrand, France. Hop Jean Minjoz, F-25030 Besancon, France. TC 4 PD NOV 16 PY 2001 VL 98 IS 11 PN Part 1 BP 861A EP 861A UT ISI:000172134103586 ER PT J AU Abraham, P Picquet, J Bickert, S Papon, X Jousset, Y Saumet, JL Enon, B TI Infra-stellate upper thoracic sympathectomy results in a relative bradycardia during exercise, irrespective of the operated side SO EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY DE sympathectomy; collateral effects; palmar hyperhidrosis; complications; exercise; bicycle; heart rate ID ENDOSCOPIC TRANSTHORACIC SYMPATHICOTOMY; ESSENTIAL HYPERHIDROSIS; TERM AB Objective: Removal of accessory fibres coming from the sub-stellar thoracic chain to the heart during infra-stellate surgical upper thoracic sympathectomy (ISS) may be responsible for a decreased heart rate to workload relationship during exercise following surgery. We hypothesised that heart rate would decrease not only following right ISS. Methods: We performed repeated bicycle incremental exercise tests in 11 control subjects (26.9 +/- 9.5 years, 61.4 +/- 12.4 kg, 167 +/- 10 cm), and 11 patients (29.8 +/- 10 years, 59.3 +/- 12.0 kg, 168 +/- 7 cm) referred for bilateral ISS: results are mean standard deviation. Surgery was performed at two distinct times allowing to study the consequences of unilateral and bilateral sympathectomy to confirm whether a significant relative bradycardia was constant and dependent on the operated side. Results: For control subjects, test durations were 13.55 +/- 3.29, 14.09 +/- 4.01 and 13.00 +/- 3.26 min and heart rates were 187 +/- 7, 187 +/- 8 and 186 +/- 7 beats min(-1) at the first, second and third test, respectively. Although time to exhaustion was comparable to controls and unchanged between tests: 12.32 +/- 2.87, 12.3 +/- 2.90, 12.33 +/- 3.76 min, heart rate at maximum exercise decreased significantly from 176 +/- 16 to 164 +/- 15, and 148 +/- 15 beats min(-1), before, following unilateral and bilateral ISS, respectively. The operated side did not allow for the prediction of the effect of unilateral sympathectomy. Conclusions: Patients should be informed of the exercise bradycardia resulting from ISS, although clinical tolerance seems excellent in endurance exercise. Contrary to previous reports at rest, during exercise no right-sided dominance was observed. These findings are consistent with reports of random distribution of sub-stellate cardiac fibres from anatomical studies. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Hosp, Dept Vasc Invest & Sports Med, F-49033 Angers 01, France. Univ Hosp, Dept Cardio Vasc & Thorac Surg, F-49033 Angers, France. RP Abraham, P, Univ Hosp, Dept Vasc Invest & Sports Med, F-49033 Angers 01, France. TC 4 PD DEC PY 2001 VL 20 IS 6 BP 1095 EP 1100 UT ISI:000172732100002 ER PT J AU Bahloul, R TI Algorithm for computing Bernstein-Sato ideals associated with a polynomial mapping SO JOURNAL OF SYMBOLIC COMPUTATION AB Let f(1),..., f(p) be polynomials in n variables with coefficients in a field K. We associate with these polynomials a number of functional equations and related ideals B, B-j and B-Sigma of K[s(1),...,s(p)] called Bernstein-Sato ideals. Using standard basis techniques, our aim is to present an algorithm for computing generators of B-j and B-Sigma. (C) 2001 Academic Press. C1 Univ Angers, Dept Math, F-49045 Angers, France. RP Bahloul, R, Univ Angers, Dept Math, 2 Bd Lavoisier, F-49045 Angers, France. TC 4 PD DEC PY 2001 VL 32 IS 6 BP 643 EP 662 UT ISI:000172441800005 ER PT J AU Boudebs, G Sanchez, F Duverger, C Boulard, B TI Improvement of Mach-Zehnder interferometry technique for third-order susceptibility measurement SO OPTICS COMMUNICATIONS DE Mach-Zehnder interferometer; nonlinear refractive index; Fourier transform; third-order susceptibility ID NONLINEAR REFRACTIVE-INDEX; FLUORIDE GLASSES AB We present in this paper an improved method to measure the third-order nonlinearity of poor optical quality samples. The principle is based on a pump/probe experiment using a Mach-Zehnder interferometer coupled to a CCD camera. The method first consists of recording the interference pattern in absence of pump beam thus allowing to characterize the linear properties of the sample. This acquisition is then compared to the interference pattern when the pump beam generates a nonlinear dephasing. From these data we can extract the nonlinear index coefficient. Therefore poor optical quality samples can be characterized because either inhomogeneities or surface defects induce linear amplitude or phase variations. (C) 2001 Published by Elsevier Science BY. C1 Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, UMR 6163, F-49045 Angers 01, France. Univ Maine, CNRS, Lab Fluorures, UMR 6010, F-72085 Le Mans, France. RP Boudebs, G, Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, UMR 6163, 2 Blvd Lavoisier, F-49045 Angers 01, France. TC 4 PD NOV 15 PY 2001 VL 199 IS 1-4 BP 257 EP 265 UT ISI:000172176300031 ER PT J AU Flunker, S Aube, C Anglade, E Vuillemin, E Bourree, Y Burtin, P Caron-Poitreau, C TI Contribution of tissue harmonic imaging in biliary lithiasis SO GASTROENTEROLOGIE CLINIQUE ET BIOLOGIQUE DE tissue harmonic imaging; gallbladder stone; biliary tract ID COMMON BILE-DUCT; ENDOSCOPIC RETROGRADE CHOLANGIOPANCREATOGRAPHY; ULTRASOUND; SONOGRAPHY; AGREEMENT AB Aim of the study-To define the role of harmonic imaging in the study of biliary lithiasis. Subjects and methods-We included 50 consecutive patients with suspected biliary lithiasis. Three independent observers compared results of harmonic imaging and conventional ultrasonography. Results-This study showed a better interobserver agreement with harmonic imaging for acoustic shadow (kappa = 0.87 vs; 0.68) and intra-hepatic biliary stones (kappa = 0.79 vs 0.49). More stones and more lithiasic gallbladders were seen with harmonic imaging (27 vs 24) and visualization of gallbladder sludge and the acoustic shadow from stones (P=0.01) was better. Ultrasound examination appears to be easier and faster and the diagnosis is more certain with harmonic imaging than with conventional ultrasonography (P=0.005). Conclusion-Harmonic imaging provides more information on biliary lithiasis and a more certain diagnosis. C1 CHU Angers, Serv Radiol, F-49033 Angers 01, France. CHU Angers, Serv Hepatogastroenterol, F-49033 Angers, France. RP Aube, C, CHU Angers, Serv Radiol B, 4 Rue Larrey, F-49033 Angers 01, France. TC 4 PD JUN-JUL PY 2001 VL 25 IS 6-7 BP 589 EP 594 UT ISI:000172121500003 ER PT J AU Bancewicz, T Godet, JL Maroulis, G TI Collision-induced hyper-Rayleigh spectrum of octahedral molecules: The case of SF6 SO JOURNAL OF CHEMICAL PHYSICS ID INDUCED LIGHT-SCATTERING; SULFUR-HEXAFLUORIDE; CENTROSYMMETRIC MOLECULES; RAMAN-SCATTERING; HYPERPOLARIZABILITIES; LIQUIDS; GASES; PHASE; ATOMS; PAIR AB A theoretical expression giving the collision-induced hyper-Rayleigh (CI HR) spectrum due to pairs of centrosymmetric molecules of octahedral symmetry has been derived. The dipole2-quadrupole hyperpolarizability light scattering mechanism of collision-induced hyperpolarizability Delta beta (LM) is discussed in detail and proposed to explain the CI HR spectrum. Numerically we have applied our analytical formulas to binary CI HR spectrum of sulfur-hexafluoride. We have normalized our SF6 CI HR spectrum to the monomer HR spectrum of CCl4. The spectral contribution due to second hyperpolarizability-permanent hexadecapole HR light scattering mechanism has been estimated and showed to be negligible. (C) 2001 American Institute of Physics. C1 Adam Mickiewicz Univ Poznan, Inst Phys, Div Nonlinear Opt, PL-61614 Poznan, Poland. Univ Angers, Lab Proprietes Opt Mat & Applicat, F-49045 Angers, France. Univ Patras, Dept Chem, Phys Chem Lab, GR-26500 Patras, Greece. RP Bancewicz, T, Adam Mickiewicz Univ Poznan, Inst Phys, Div Nonlinear Opt, Umultowska 85, PL-61614 Poznan, Poland. TC 4 PD NOV PY 2001 VL 115 IS 18 BP 8547 EP 8551 UT ISI:000171796800033 ER PT J AU Benghanem, F Chafaa, S Bouet, GM Khan, MA TI Potentiometric studies of 4-mono(dihydroxyphosphonyl) methylphenol and 2,6-bis(dihydroxyphosphonyl) methyl 4-methyl phenol complexes with cobalt(II), nickel (II), copper (II), zinc (II) and cadmium (II) SO PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS DE hydroxyphenyl phosphonates; complexes; aqueous solution; stability ID (O-HYDROXYPHENYL)METHYLPHOSPHONIC ACIDS; MULTIDENTATE LIGANDS; PKA VALUES AB The overall stability of the two acids: 4-mono(dihydroxy-phosphonyl) methyl phenol (LI) and 2,6-bis(dihydroxyphosphonyl)methyl 4-methyl phenol (L2) complexes with five divalent metal cations Co-II, Ni-II Cu-II, Zn-II and Cd-II were determined patentiometrically at constant ionic strength of 0.1 mol.L-1 and at 25.0 +/- 0.1 degreesC (298.0 +/- 0.1 K) in water. The values are as follows. For L1: Ni-II, 5.27; Co-II, 5.65; Cu-II, 7.69; Zn-II, 7.39 and Cd-II, 5.75. For L2: Ni-II, 7.24; Co-II, 7.47; Cu-II, 12.76; Zn-II, 11.39 and Cd-II, 7.94. C1 Fac Pharm Angers, F-49100 Angers, France. Univ Ferhat Abbas, Inst Chim Ind, Setif 19000, Algeria. RP Bouet, GM, Fac Pharm Angers, 16 Blvd Daviers, F-49100 Angers, France. TC 4 PY 2001 VL 170 BP 159 EP 169 UT ISI:000171514700013 ER PT J AU Boudou, P Taieb, J Mathian, B Badonnel, Y Lacroix, I Mathieu, E Millot, F Queyrel, N Somma-Delpero, C Patricot, MC TI Comparison of progesterone concentration determination by 12 non-isotopic immunoassays and gas chromatography/mass spectrometry in 99 human serum samples SO JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY DE gas-chromatography; immunoassays; mass-spectrometry; progesterone ID FERTILIZATION-EMBRYO TRANSFER; HUMAN CHORIONIC-GONADOTROPIN; MASS-SPECTROMETRY; ECTOPIC PREGNANCY; INVITRO FERTILIZATION; PREMENSTRUAL-SYNDROME; PLASMA PROGESTERONE; LEUPROLIDE ACETATE; CIRCULATING LEVELS; 5-ALPHA-PREGNANE-3,20-DIONE AB A single serum progesterone determination may be highly predictive for early pregnancy and in vitro fertilisation and embryo-transfer outcomes. We therefore compared 12 direct non-isotopic progesterone immunoassays with gas-chromatography/mass spectrometry (GC/MS). For each assay, data from the analysis of 99 individual sera were compared with data obtained by GC/MS, using regression and bias plot analyses and the ratio method. We observed a larger difference in concentration between high and low values and a broader distribution of results for immunoassays than for GC/MS. All immunoassays displayed bias in the calibration process and a lack of specificity and/or sensitivity, to various degrees. We tried to identify the parameters of the assay procedure that might contribute to these discrepancies. None of the criteria investigated (antibodies, control and preparation of calibrators, blocking agents and choice of tracer) had a significant effect when studied alone. (C) 2001 Elsevier Science Ltd. All rights reserved. C1 Hop St Louis, Dept Hormonal Biol, F-75010 Paris, France. Hop Antoine Beclere, Hormonol Lab, Clamart, France. Ctr Hosp Lyon Sud, Hormonol Lab, Lyon, France. A Pinard Hosp, Biochem Lab, Nancy, France. CERBA Lab, Cergy, France. Angers Hosp, Mol Biol Lab, Angers, France. Hop Tenon, Biochem & Hormonol Lab, F-75970 Paris, France. Versailles Hosp, Hormonol Lab, Le Chesnay, France. Fac Med Marseille, Endocrinol Lab, F-13385 Marseille, France. RP Boudou, P, Hop St Louis, Dept Hormonal Biol, 1 Ave Claude Vellefaux, F-75010 Paris, France. TC 4 PD JUL PY 2001 VL 78 IS 1 BP 97 EP 104 UT ISI:000171265900013 ER PT J AU Assi, A Castro-Jimenez, FJ Granger, M TI The analytic standard fan of a D-module SO JOURNAL OF PURE AND APPLIED ALGEBRA ID GROBNER FAN; SLOPES AB In this paper, we associate with any monogeneous module over the ring D of germs of linear differential operators at the origin of C-n, with holomorphic coefficients, a combinatorial object which we call the standard fan of this D-module (see Section 6 for a precise geometric description of this object). The main tool of the proof is the homogenization technique and a convergent division theorem in the homogenization ring D[t]. This last result is the key tool to an extension to the analytic D-module case of our results in the algebraic case of the Weyl algebra (see Assi et al., J. Pure Appl. Algebra, 150 (1) (2000) 27-39. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, F-49045 Angers 01, France. Univ Sevilla, Fac Matemat, Dept Algebra, Seville, Spain. RP Granger, M, Univ Angers, Bd Lavoisier, F-49045 Angers 01, France. TC 4 PD OCT 24 PY 2001 VL 164 IS 1-2 BP 3 EP 21 UT ISI:000171099100002 ER PT J AU Sahraoui, B Kityk, IV Fuks, I Paci, B Baldeck, P Nunzi, JM Frere, P Roncali, J TI Novel nonlinear optical organic materials: Dithienylethylenes SO JOURNAL OF CHEMICAL PHYSICS ID TETRATHIAFULVALENE; ABSORPTION; GENERATION; OLIGOMERS AB In this paper, we report the measurements of the third-order susceptibilities of new dithienylethylenes in solutions using the degenerate four-wave mixing technique at lambda =532 nm in the picosecond regime. From these measurements we deduced the second-order hyperpolarizabilities gamma values, which are about 10(3) larger than the gamma value of CS2 and are comparable with the corresponding values for the new synthesized diphenylpolyene derivatives. We also determined the optical power limiting properties of the mentioned compounds using nanosecond laser pulses in the visible range. The dispersion of nonlinear absorption coefficients is obtained in the framework of Gaussian collimated beam propagation in sample. Kerr ellipsometry measurements are responsible for the excited absorption cross section and are spectrally shifted comparing with the optical limiting processes (up to 50 nm). The appropriate quantum chemical calculations are done. The contributions of electronic and vibration subsystems as well as contribution of the particular parts of each molecule into the output third nonlinear optical response are given. (C) 2001 American Institute of Physics. C1 Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, UMR 6136, F-49045 Angers, France. Inst Phys WSP, PL-42201 Czestochowa, Poland. Univ Angers, Lab Ingn Mol & Mat Organ, CNRS, UMR 6501, F-49045 Angers, France. RP Sahraoui, B, Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, UMR 6136, 2 Blvd Lavoisier, F-49045 Angers, France. TC 4 PD OCT 1 PY 2001 VL 115 IS 13 BP 6179 EP 6184 UT ISI:000171015100051 ER PT J AU Pichon, MF Basuyau, JP Gory-Delabaere, G Eche, N Daver, A Blanc-Vincent, MP Riedinger, JM Deneux, L Bidart, JM TI Standards, Options and Recommendations for tumor markers in thyroid cancers SO BULLETIN DU CANCER DE guidelines; thyroglobulin; calcitonin; thyroid neoplasms; tumor markers; carcinoembryonic-antigen ID SERUM THYROGLOBULIN MEASUREMENT; PENTAGASTRIN STIMULATION TEST; MESSENGER-RIBONUCLEIC-ACID; GENE-RELATED PEPTIDE; WHOLE-BODY SCAN; FOLLOW-UP; CARCINOEMBRYONIC ANTIGEN; IMMUNORADIOMETRIC ASSAY; ROUTINE MEASUREMENT; PERIPHERAL-BLOOD AB Context: The "Standards, Options and Recommendations" (SOR) project, started in 1993, is a collaboration between the Federation of the French Cancer Centers (FNCLCC), the 20 French Cancer Centers and specialists from French Public Universities, General Hospitals and Private Clinics. The main objective is the development of clinical practice guidelines to improve the quality of health care and outcome-for cancer patients. The methodology is based on literature review and critical appraisal by a multidisciplinary group of experts, with feedback from specialists in cancer care delivery. Objectives: To define, according to the definitions of the Standards, Options and Recommendations project, the characteristics of the main tumor markers in thyroid cancer and the potential role of these markers in the management of patients with this malignancy. Methods: Data were identified hy searching Medline (R) and the personal reference lists of members of the expert groups. Once the guidelines were defined, the document was submitted for review to 55 independent reviewers, and to the medical committees of the 20 French Cancer Centers. Results: The main recommendations are: 1) Thyroglobulin is a serum tumor marker for the monitoring of operated thyroid differentiated neoplasms (standard). 2) It is essential to know if the patient is under TSH stimulation or under thyroid suppression therapy to interpret thyroglobulin results (standard). 3) Thyroglobulin assay must be performed regularly during the monitoring of differentiated thyroid neoplasms (standard, level of evidence B2), should be coupled with the measurement Of anti-thyroglobulin antibodies concentration using a sensitive method (standard level of evidence B2). 4) Thyroglobulin assay should not be performed to detect or diagnose differentiated thyroid neoplasms (standard, level of evidence B2). 5) The methods used to assay thyroglobulin must have a limit of detection lower than 3 mug.l(-1) (standard, expert agreement). 6) Calcitonin is a marker for medullary thyroid cancer (standard). 7) Its assay, associated with RET gene study if indicated, enables medullary thyroid cancer to be diagnosed 8) The pentagastrin test is essential to diagnose familial forms of medullary thyroid cancer. 9) All analyses for each patient must be performed in the same laboratory, using the same technique (standard, expert agreement). 10) Calcitonin and carcinoembryonic-antigen are serum markers for the monitoring of medullary thyroid cancer and allow the detection of recurrent disease (standard). C1 Ctr Rene Huguenin, St Cloud, France. Ctr Henri Becquerel, F-76038 Rouen, France. FNCLCC, Paris, France. Inst Claudis Regaud, Toulouse, France. Ctr Paul Papin, Angers, France. Ctr Georges Francois Leclerc, Dijon, France. Inst Curie, Paris, France. Inst Gustave Roussy, Villejuif, France. RP Pichon, MF, Ctr Rene Huguenin, St Cloud, France. TC 4 PD AUG PY 2001 VL 88 IS 8 BP 775 EP 792 UT ISI:000171138300009 ER PT J AU Ter Minassian, A Beydon, L Ursino, M Gardette, B Gortan, C Richalet, JP TI Doppler study of middle cerebral artery blood flow velocity and cerebral autoregulation during a simulated ascent of Mount Everest SO WILDERNESS & ENVIRONMENTAL MEDICINE DE hypoxia; brain; hemodynamics; Doppler (transcranial) ID HIGH-ALTITUDE; HYPEREMIC RESPONSE; EXTREME ALTITUDE; VASODILATION; SICKNESS; HYPOXIA; INDEX; MEN AB Objective.-To explore cerebral hemodynamics in 8 healthy volunteers in a hypobaric chamber up to the altitude of Mount Everest after a progressive stepwise decompression to 8848 in. Methods.-Physiological, clinical, and transcranial Doppler data were collected after at least 3 days at 5000, 6000, and 7000 m and within 4 hours of reaching 8000 m and returning to sea level. Results.-Three subjects were excluded at 8000 and 8848 in because of acute neurological deficits. Heart rate increased; mean arterial pressure remained stable; PaO2 and PaCO2 decreased with altitude; hemoglobin (Hb) and hematocrit (Ht) increased; arterial O-2 content decreased over 6000 m; middle cerebral artery blood flow velocity (MCAv) increased only during acute exposure to 8000 m; and the corresponding pulsatility (PI) and resistivity indices (RI) decreased over 5000 m. PI and RI correlated with heart rate. The transient hyperemic response (THR) of MCAv to common carotid compression was depressed at 8000 m. Conclusions.-At 8000 m, the increase in MCAv seemed to reflect the normal hemodynamic response to acute hypoxia. The decrease of THR at this altitude could be an indication of impaired cerebral autoregulation. The role of impaired cerebral autoregulation in the genesis of acute neurologic deficits, observed at 8000 m and above in 3 subjects, remains speculative. C1 CHU Angers, Dept Anesthesie Reanimat, F-49033 Angers, France. Univ Bologna, Dipartimento Elettron Informat & Sistemist, I-40136 Bologna, Italy. COMEX, F-13009 Marseille, France. Univ Paris 13, Lab Reponses Cellulaires & Fonctionnelles Hypoxie, ARPE, F-93017 Bobigny, France. RP Ter Minassian, A, CHU Angers, Dept Anesthesie, F-49033 Angers, France. TC 4 PD FAL PY 2001 VL 12 IS 3 BP 175 EP 183 UT ISI:000170982900003 ER PT J AU Veal, N Moal, F Wang, JH Vuillemin, E Oberti, F Roy, E Kaassis, M Trouve, R Saumet, JL Cales, P TI New method of cardiac output measurement using ultrasound velocity dilution in rats SO JOURNAL OF APPLIED PHYSIOLOGY DE transit time ultrasound method; radiolabeled microsphere method; portal hypertension; terlipressin; losartan ID PORTAL-HYPERTENSIVE RATS; REGIONAL BLOOD-FLOW; TRANSIT-TIME ULTRASOUND; COLLATERAL CIRCULATION; VALIDATION; FLOWMETER; TERLIPRESSIN; PROPRANOLOL AB The aim of this study was to validate a new technique for the measurement of cardiac output (CO) based on ultrasound and dilution (COUD) in anesthetized rats. A transit time ultrasound (TTU) probe was placed around the rat carotid artery, and ultrasound velocity dilution curves were generated on intravenous injections of saline. CO by COUD were calculated from the dilution curves for normal and portal hypertensive rats in which CO was known to be increased. COUD was compared with the radiolabeled microsphere method and with direct aortic TTU flowmetry for baseline CO and drug-induced CO variations. CO in direct aortic TTU flowmetry was the ascending aorta blood flow measured directly by TTU probe (normal use of TTU flowmetry). The reproducibility of COUD within the same animal was also determined under baseline conditions. COUD detected the known CO increase in portal hypertensive rats compared with normal rats. CO values by COUD were correlated with those provided by microsphere technique or direct aortic TTU flowmetry (adjusted r = 0.76, P< 10(-4) and r = 0.79, P< 0.05, respectively). Baseline CO values and terlipressin-induced CO variations were detected by COUD and the other techniques. Intra- and interobserver agreements for COUD were excellent (intraclass r = 0.99 and 0.98, respectively). COUD was reproducible at least 10 times in 20 min. COUD is an accurate and reproducible method providing low-cost, repetitive CO measurements without open-chest surgery. It can be used in rats as an alternative to the microsphere method and to direct aortic flowmetry. C1 Univ Angers, Lab HIFIH, F-49033 Angers, France. Univ Angers, Physiol Lab, UPRES EA 2170, F-49033 Angers, France. RP Cales, P, CHU Angers, Serv Hepatogastroenterol, F-49033 Angers 01, France. TC 4 PD SEP PY 2001 VL 91 IS 3 BP 1274 EP 1282 UT ISI:000170552800034 ER PT J AU Sauve, G Croue, A Denez, B Boyer, J TI High-grade dysplasia in heterotopic gastric mucosa in the upper esophagus after radiotherapy: Successful eradication 2 years after endoscopic treatment by argon plasma coagulation SO ENDOSCOPY C1 Univ Hosp Angers, Gastroenterol Unit, F-49000 Angers, France. Univ Hosp Angers, Pathol Unit, F-49000 Angers, France. RP Boyer, J, Univ Hosp Angers, Gastroenterol Unit, F-49000 Angers, France. TC 4 PD AUG PY 2001 VL 33 IS 8 BP 732 EP 732 UT ISI:000170366300021 ER PT J AU Geze, A Venier-Julienne, MC Cottin, J Faisant, N Benoit, JP TI PLGA microsphere bioburden evaluation for radiosterilization dose selection SO JOURNAL OF MICROENCAPSULATION DE PLGA; microsphere; bioburden; radiosterilization; solvent emulsion/extraction technique ID GAMMA-IRRADIATION; RADIATION STERILIZATION; POLYMERS; DEGRADATION; MICROPARTICLES; DELIVERY; SYSTEM; ACID) AB The aim of this study was to determine the bioburden of PLGA microspheres produced by the solvent emulsion/extraction process as a means of determining an appropriate gamma -irradiation dose for sterilization. Bioburden was evaluated on the basis of ISO specifications. The analysis of initial microbial contamination was performed on blank microspheres, prepared by a non-aseptic laboratory scale process. A mean bioburden of 36.04 CFU (colony forming units)/110 mg microspheres was determined. Most of the detected germs originated from human commensal flora. According to the ISO dose-selection method, a gamma -irradiation dose of 19.6 kGy was found sufficient to ensure a sterility level of 10(6). The effect of the selected irradiation dose on both the molecular weight of the polymer and the kinetics of 5-fluorouracil drug release from the microspheres was compared to the European Pharmacopeia recommended irradiation dose (25 kGy). This 20% reduced dose showed a lower extent of molecular weight reduction of PLGA and a better control of 5-FU release from microparticles. This can be related to reduce polymer radiation damage. C1 Fac Pharm, UPRES EA 2169, F-49045 Angers, France. CHU Angers, Bacteriol Lab, F-49033 Angers, France. RP Benoit, JP, Fac Pharm, UPRES EA 2169, 16 Bd Daviers, F-49045 Angers, France. TC 4 PD JUL PY 2001 VL 18 IS 5 BP 627 EP 636 UT ISI:000170240400008 ER PT J AU Weber, JFF Wahab, IA Marzuki, A Thomas, NF Kadir, AA Hadi, AHA Awang, K Latiff, AA Richomme, P Delaunay, J TI Heimiol A, a new dimeric stilbenoid from Neobalanocarpus heimii SO TETRAHEDRON LETTERS AB A new stilbene dimer, heimiol A was isolated from the heartwood of Neobalanocarpus heimii. in addition to the four known oligostilbenoids, balanocarpol. copalliferol A, hopeaphenol and vaticaphenol A. The structures and relative configuration were established by means of 2D NMR spectroscopy including COSY, HMQC, HMBC and NOESY. (C) 2001 Published by Elsevier Science Ltd. C1 Univ Kebangsaan Malaysia, Fac Allied Hlth Sci, Dept Pharm, Kuala Lumpur 50300, Malaysia. FRIM, Kepong 52109, Malaysia. Univ Malaya, Fac Sci, Dept Chem, Kuala Lumpur 59100, Malaysia. Univ Sains Malaysia, Doping Control Ctr, Penang 11800, Malaysia. SCRMN, Fac Sci, F-49045 Angers, France. RP Weber, JFF, Univ Kebangsaan Malaysia, Fac Allied Hlth Sci, Dept Pharm, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. TC 4 PD JUL 16 PY 2001 VL 42 IS 29 BP 4895 EP 4897 UT ISI:000169715900038 ER PT J AU Libouban, H Moreau, MF Lesourd, M Basle, MF Chappard, D TI Osteolytic bone lesions in the 5T2 multiple myeloma model: Radiographic, scanning electron microscopic, and microtomographic studies SO JOURNAL OF HISTOTECHNOLOGY DE animal model; bone resorption; trabecular ID C57BL/KALWRIJ MOUSE; CELLS AB Multiple myeloma (MM) is a B-cell malignancy characterized by a monoclonal expansion of plasma cells within the bone marrow, the presence of a monoclonal serum immunoglobulin, and the activation of osteoclastic resorption leading to osteolytic lesions. An animal model mimicking human MM consists of grafting 5T2MM cells in the C57BL/KaLwRij mouse. We have inoculated 5T2MM cells intravenously into young recipient mice. The development of the disease was monitored 9 wk later by serum electrophoresis to detect the presence of a paraprotein. Mice were killed 16 wk after inoculation of the 5T2 cells. Osteolytic lesions were analyzed by 3 different methods: numeric radiography using a Faxitron machine, SEM, and X-ray microtomography that provides 3D information and reconstruction. The first 2 methods were used to quantify bone lesions and resorption. We observed the presence of numerous small resorption cavities in the long bones on the endosteal side. The most severe lesions were localized in the metaphysis of the femur and tibia. In the 5T2MM mouse, bone surfaces undergoing resorption were significantly increased on the endosteum of the femur shaft (control: 19.2%, 5T2MM: 76.2%, p < 0.0001). This study revealed that osteolytic lesions develop both at the endosteal and trabecular level with cortical perforations occurring at the terminal stage of the disease. C1 Serv Commun Imagerie & Micro Anal, Lab Histol Embryol, Angers, France. RP Chappard, D, Fac Med, LHEA, F-49045 Angers, France. TC 4 PD JUN PY 2001 VL 24 IS 2 BP 81 EP 86 UT ISI:000169480100002 ER PT J AU Demotes-Mainard, S Jeuffroy, MH TI Incorporating radiation and nitrogen nutrition into a model of kernel number in wheat SO CROP SCIENCE ID GRAIN-YIELD; WINTER-WHEAT; SIMULATION-MODEL; SPRING WHEAT; CROP; GROWTH; TEMPERATURE; MANAGEMENT; COMPONENTS; FERTILIZER AB Wheat (Triticum aestivum L.) kernel number per square meter (KN) depends on radiation, temperature, and crop N nutrition. Some models of KN using climatic data or N nutrition characteristics as input variables account for variations either in radiation and temperature or in N nutrition, but not for both. Our objective was to produce a model of KN that accounts simultaneously for variations due to radiation, temperature, and crop N nutrition, and that has input variables that are simple to measure or to simulate. Field experiments were conducted over 3 yr with 'Tremie' winter wheat. Treatments involved the application of N fertilizer at different dates and rates to achieve various N deficiencies and the use of shading nets for various periods during spike growth to reduce incident radiation. Crop N status was assessed by determining N nutrition index (NNI) at anthesis. The KN was counted, it ranged from 9420 to 31036 kernels m(-2). Two characteristics of N nutrition (NNI at anthesis and IDD, the duration of deficiency before anthesis multiplied by its intensity) and three characteristics of radiation and temperature (photothermal quotient calculated from 45 d before anthesis to anthesis, from 30 d before anthesis to anthesis and from 20 d before anthesis to 10 d after anthesis) were used as input variables. Six relationships combining one characteristic of N nutrition and the photothermal quotient over one period were estimated. The best fit was obtained for a relationship between KN and the logarithm of NNI at anthesis and photothermal quotient over the 45 d preceding anthesis (R-2 = 0.883, n = 19). This relationship could be useful for estimating KN in crop models, as its input variables are simple to simulate. C1 INRA, INA PG, UMR Agron, F-78850 Thiverval Grignon, France. Univ Angers, INH, UMR SAGAH, INRA, F-49071 Beaucouze, France. RP Jeuffroy, MH, INRA, INA PG, UMR Agron, BP 01, F-78850 Thiverval Grignon, France. TC 4 PD MAR-APR PY 2001 VL 41 IS 2 BP 415 EP 423 UT ISI:000169469800020 ER PT J AU Bremond, A Bataillard, A Thomas, L Achard, JL Fervers, B Fondrinier, E Lansac, J Bailly, C Hoffstetter, S Basuyau, JP d'Anjou, J Descamps, P Farsi, F Guastalla, JP Laffargue, F Rodier, JF Vincent, P Pigneux, J TI Cancer of the endometrium SO BRITISH JOURNAL OF CANCER C1 Ctr Leon Berard, F-69373 Lyon, France. FNCLCC, Paris, France. Inst Bergonie, Bordeaux, France. Ctr Jean Perrin, Clermont Ferrand, France. Ctr Paul Papin, Angers, France. CHU Bretonneau, F-37044 Tours, France. Ctr Alexis Vautrin, Nancy, France. Ctr Henri Becquerel, F-76038 Rouen, France. CHU Angers, Angers, France. Hop Arnaud de Villeneuve, CHU, Montpellier, France. Ctr Paul Strauss, Strasbourg, France. Clin St Catherine, Avignon, France. RP Bremond, A, Ctr Leon Berard, F-69373 Lyon, France. TC 4 PD MAY PY 2001 VL 84 SU Suppl. 2 BP 31 EP 36 UT ISI:000169105700008 ER PT J AU Ligot, J Benayoun, S Hantzpergue, JJ TI Characterization and modeling of electrical resistivity of sputtered tungsten films SO JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS ID THIN METAL-FILMS; MICROSTRUCTURE; STRESSES; DEPOSITION; POLYIMIDE AB Tungsten (W) thin films were deposited on a polyimide substrate by sputtering deposition inn pure argon plasma using a triode discharge system. The properties of these W films were investigated as functions of ions energy, argon pressure and film thickness. The composition and crystallographic and micrographic structures of W films were determined by Rutherford backscattering spectroscopy and x-ray diffraction techniques, respectively. Less than 6 at. % argon and metallic impurities (duc to the sputtering chamber configuration) was incorporated into the W films and different volume ratios of tungsten crystallographic phases (Wa and WP) were observed in the films. The electrical resistivity of Wa films was interpreted from impurity concentrations and the microstructure with a model based on electron scattering mechanisms at grain boundaries. It was shown that the electron reflectivity of the grain boundaries depends of the size of the grains. In order to evaluate the contribution of wp phase on the electrical resistivity of biphase W films, two models were proposed. The model of the averaged effect of the two phases leads to calculations that are in good agreement with experimental results and an intrinsic resistivity of WP phase is proposed to be close to 30 mu Omega cm. (C) 2001 American Vacuum Society. C1 CER Angers, ENSAM, Lab Proc Mat Instrumentat, F-49035 Angers, France. RP Benayoun, S, CER Angers, ENSAM, Lab Proc Mat Instrumentat, 2 Blvd Ronceray,BP 3525, F-49035 Angers, France. TC 4 PD MAY-JUN PY 2001 VL 19 IS 3 BP 798 EP 804 UT ISI:000168922300013 ER PT J AU Blanchard, P Raimundo, JM Roncali, J TI New synthetic strategies towards conjugated NLO-phores and fluorophores SO SYNTHETIC METALS DE heterocycle synthesis; UV-Vis-NIR absorption; photoluminescence; polythiophene and derivatives; low-bandgap conjugated polymers ID POLYMERS; BANDGAP AB Thermally stable push-pull chromophores built around thiophene-based rr-conjugating spacers rigidified by covalent bridging are described. We show that rigidification of the spacer produces a huge enhancement of the quadratic nonlinear susceptibility which reaches values among the highest known to date. In addition, we show that introduction of proquinoid accepters as building blocks for the design of conjugated fluorophores leads to new systems combining tunable emission wavelength at constant geometry, emission quantum yields close to unity, low oxidation potential and high electron affinity. C1 Univ Angers, UMR CNRS 6501, F-49045 Angers, France. RP Roncali, J, Univ Angers, UMR CNRS 6501, 2 Blvd Lavoisier, F-49045 Angers, France. TC 4 PD MAR 15 PY 2001 VL 119 IS 1-3 SI Sp. Iss. SI BP 527 EP 528 UT ISI:000168741500238 ER PT J AU Debenay, JP Duleba, W Bonetti, C Souza, SHDME Eichler, BB TI Pararotalia cananeiaensis n. sp.: Indicator of marine influence and water circulation in Brazilian coastal and paralic environments SO JOURNAL OF FORAMINIFERAL RESEARCH ID BENTHIC FORAMINIFERA; POLLUTION; ASSEMBLAGES; AQUACULTURE; POPULATIONS; TRANSPORT; LAGOONS; INLET AB Pararotalia cananeiaensis, a new rotaliid species from modern shelf sediments of southern Brazil is described, This small species is well-represented along the southern coast of Brazil. It was recorded for the first time in the laguno-estuarine complex of Cananeia-Iguape in 1995, It had never been reported before, probably because it was confused with juveniles of Ammonia, Its distribution has been studied in Brazilian coastal and paralic environments from Cabo Frio (Rio de Janeiro state) near latitude 22 degrees 50'S, to Florianopolis (Santa Catarina state) near latitude 27 degrees 35'S, Very small tests of Pararotalia cananeiaensis are easily suspended in Brazilian high energy coastal environments and are transported inward by tidal currents. Their presence in the sedimentary record can be used to gauge the extent of the effects of this transport mechanism in the estuaries of southern Brazil. C1 Univ Angers, Geol Lab, Ile Dyeu, France. Univ Angers, LEBIM, Ile Dyeu, France. Univ Sao Paulo, Inst Biociencias, Dept Ecol Geral, BR-05508900 Sao Paulo, SP, Brazil. Univ Fed Santa Catarina, Lab Oceanog Costeira CFH, BR-88040900 Florianopolis, SC, Brazil. Univ Fed Parana, Dept Geol, Ctr Politecn, BR-80060000 Curitiba, Parana, Brazil. Univ Sao Paulo, Inst Oceanof, BR-05508900 Sao Paulo, SP, Brazil. RP Debenay, JP, Univ Angers, Geol Lab, Ile Dyeu, France. TC 4 PD APR PY 2001 VL 31 IS 2 BP 152 EP 163 UT ISI:000168841000007 ER PT J AU Zotti, G Zecchin, S Schiavon, G Berlin, A Huchet, L Roncali, J TI Electrochemical synthesis and characterization of poly(4,4 '(5 ')-bis[(3,4-(ethylenedioxy)thien-2-yl] tetrathiafulvalene) films - A new TTF-bithiophene regular copolymer SO JOURNAL OF ELECTROANALYTICAL CHEMISTRY DE tetrathiafulvalene; polythiophenes; conducting polymers; electrochemistry ID MODIFIED CONDUCTING POLYMERS; ELECTRON-SPIN-RESONANCE; PI-CONJUGATED POLYMERS; MAGNETIC-PROPERTIES; POLYTHIOPHENES; ELECTROSYNTHESIS; DONOR; UNIT AB Anodic coupling of 4,4'(5')-bis[(3,4-(ethylenedioxy)thien-2-yl)]tetrathiafulvalene (1) in acetonitrile has produced a new polythiophene-based polymer containing tetrathiafulvalene (TTF) moieties regularly inserted in the polythiophene chain. The polymer poly(1) has been compared with poly(3-[7-oxa-8-(4-tetrathiafulvalenyl)octyl]-2,2'-bithiophene) bearing the TTF moieties as pendant groups. The polymer films were characterized by cyclic voltammetry, FTIR reflection-absorption and UV-vis spectroscopy, in situ ESR and in situ conductivity. The reversible oxidation of the polymers in two one-electron steps at the TTF moiety displays characteristics which suggest a faster electron transfer and a higher redox conductivity for poly(1). (C) 2001 Elsevier Science B.V. All rights reserved. C1 CNR, Ist Polarog & Elettrochim Preparat, I-35020 Padua, Italy. CNR, Ctr Sintesi & Sterochim Special Sistemi Organ, I-20133 Milan, Italy. Univ Angers, Ingn Mol & Mat Organ, CNRS, UMR 6501, F-49045 Angers, France. RP Zotti, G, CNR, Ist Polarog & Elettrochim Preparat, Corso Stati Uniti 4, I-35020 Padua, Italy. TC 4 PD MAY 4 PY 2001 VL 504 IS 1 BP 64 EP 70 UT ISI:000168710800006 ER PT J AU Boudebs, G Chis, M Phu, XN TI Third-order susceptibility measurement by a new Mach-Zehnder interferometry technique SO JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS ID NONLINEAR REFRACTIVE-INDEX; GLASSES AB A new technique for measuring third-order susceptibility with a Mach-Zehnder interferometer with a pump-probe system is proposed. The optical setup is combined with a charge-coupled device for image processing. With the proposed method it is possible to resolve the spatial profile of a complex nonlinear variation index with only one laser shot in the nonlinear material. Therefore we can get intensity-resolved information by comparing this profile, pixel per pixel, with that of the incident beam. To verify the validity of the method, we carry out measurements of reference materials illuminated by linearly polarized light. Good agreement is obtained with measurements made by various authors. An advantage of this new technique is that only one laser shot is needed to minimize the risk of damage in fragile nonlinear materials. (C) 2001 Optical Society of America. C1 Univ Angers, CNRS, UMR 6163, Lab Proprietes Opt Mat & Applicat, F-49045 Angers 01, France. RP Boudebs, G, Univ Angers, CNRS, UMR 6163, Lab Proprietes Opt Mat & Applicat, 2 Blvd Lavoisier, F-49045 Angers 01, France. TC 4 PD MAY PY 2001 VL 18 IS 5 BP 623 EP 627 UT ISI:000168548800006 ER PT J AU Charkoudian, N Fromy, B Saumet, JL TI Reflex control of the cutaneous circulation after acute and chronic local capsaicin SO JOURNAL OF APPLIED PHYSIOLOGY DE regional blood flow; skin; vasodilation; laser-Doppler flowmetry; body temperature regulation; afferent neurons ID HUMAN FOREARM SKIN; REACTIVE HYPEREMIA; NITRIC-OXIDE; BLOOD-FLOW; VASODILATION; TEMPERATURE; RESPONSES; SMOKERS; NERVES AB To investigate whether local activity of capsaicin-sensitive sensory afferents in the skin has a modulatory role in the reflex cutaneous vasodilator response to hyperthermia in humans, experiments were conducted in two parts. First, low-dose topical capsaicin (0.025%) was administered acutely to stimulate local activity of these afferents. Second, we temporarily desensitized these nerves in a small area of skin using chronic capsaicin treatment (0.075% for 7 days). Each intervention was followed by whole body heating using water-perfused suits and then by local warming to 42 degreesC for assessment of maximum cutaneous vascular conductance. Skin blood flow was measured by laser-Doppler flowmetry and divided by mean arterial pressure (Finapres) for assessment of cutaneous vascular conductance. Maximum vascular conductance was not influenced by either acute or chronic capsaicin treatment (P > 0.10). After acute capsaicin, baseline cutaneous vascular conductance was elevated above that at control sites (25.34 +/- 6.25 vs. 10.57 +/- 2.42% max; P < 0.05). However, internal temperature thresholds for vasodilation were not affected by either acute or chronic capsaicin (P > 0.10). Furthermore, neither acute (control: 112.74 +/- 36.83 vs. acute capsaicin: 96.92 +/- 28.92% max/degreesC; P > 0.10) nor chronic (control: 142.45 +/- 61.89 vs. chronic capsaicin: 132.12 +/- 52.60% max/degreesC; P > 0.10) capsaicin administration influenced the sensitivity of the reflex cutaneous vasodilator response. We conclude that local activity of capsaicin-sensitive afferents in the skin does not modify reflex cutaneous vasodilation during hyperthermia. C1 Univ Angers, Lab Physiol & Explorat Fonctionnelles Vasc, F-49045 Angers, France. RP Charkoudian, N, Mayo Clin & Mayo Fdn, 200 1st St SW, Rochester, MN 55905 USA. TC 4 PD MAY PY 2001 VL 90 IS 5 BP 1860 EP 1864 UT ISI:000168227600030 ER PT J AU Aqad, E Leriche, P Mabon, G Gorgues, A Khodorkovsky, V TI Fulvalene derivatives: strong proaromatic electron acceptors SO TETRAHEDRON LETTERS ID ORGANIC METALS; SYSTEMS AB Synthesis and electrochemical properties of novel proaromatic electron accepters based on the fulvalene parent system are described. (C) 2001 Elsevier Science Ltd. All rights reserved. C1 Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel. Univ Angers, UMR CNRS, Lab IMMO, F-49045 Angers, France. Univ Angers, Fac Sci, F-49045 Angers, France. RP Gorgues, A, Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel. TC 4 PD APR 9 PY 2001 VL 42 IS 15 BP 2813 EP 2815 UT ISI:000168048600016 ER PT J AU Fournier, HD Mercier, P Menei, P TI Anatomical bases of the posterior approach to the brachial plexus for repairing avulsed spinal nerve roots SO SURGICAL AND RADIOLOGIC ANATOMY DE anatomy; avulsion; brachial plexus; nerve repair; reimplantation ID CORD IMPLANTATION AB Avulsion of nerve roots from the cervical spinal cord has always been considered as an untreatable injury, even by surgeons with expertise in this area. However, numerous experimental studies in animals, as well as a human case report, showed that if continuity is restored between the spinal cord and nerve roots, axons from spinal motor neurons can regrow into the peripheral nerve graft with a subsequent recovery of motor function. The posterior subscapular approach, based on the evolution of the posterolateral approach for removal of the first rib, is the only way to expose the entire brachial plexus from C5 to T1 from the ventral and dorsal roots to the distal nerve trunks. The purpose of this study is to investigate the topographic anatomy of the brachial plexus, with particular emphasis on the relationships important to the posterior approach and reimplantation of the ventral rootlets within the cord. either directly or using peripheral nerve grafts. The major advantage of the procedure is the proximal exposure of the plexus, with evaluation of the lesions being excellent (intradural, foraminal and proximal trunks). Reimplantation of ventral roots into the cord is relatively easy from C5 to C7, more difficult for CX and problematic for TI, whereas reimplantation of dorsal roots into the cord is easy from C5 to T1. The disadvantages of this approach for exposure of the plexus and nerve root avulsion repair are significant: the surgical technical steps are difficult mainly because of the cervical paraspinal muscle mass, which cannot be easily "elevated and retracted" despite previous descriptions: bleeding from the venous plexus can he excessive as suggested by dissection and our own experience: the stability of the cervical spine may be compromised following extensive laminectomy with total unilateral facetectomy; exposure of the plexus distal to the division of the trunks is difficult; there may be injury to the long thoracic nerve and subsequent winging of the scapula; and pneumothorax. This approach is therefore only applicable in highly selected cases involving multiple avulsed roots with proximal Lesions extending as far as the division of the trunks. C1 Fac Med, Lab Anat, F-49045 Angers, France. CHU Angers, Serv Neurochirurg, F-49033 Angers 01, France. RP Fournier, HD, Fac Med, Lab Anat, Rue Haute de Reculee, F-49045 Angers, France. TC 4 PD FEB PY 2001 VL 23 IS 1 BP 3 EP 8 UT ISI:000168023600002 ER PT J AU Henaff, G Tonneau, A TI Environment-sensitive fracture of iron aluminides under monotonic tensile loading SO METALLURGICAL AND MATERIALS TRANSACTIONS A-PHYSICAL METALLURGY AND MATERIALS SCIENCE ID ROOM-TEMPERATURE DUCTILITY; STRAIN-RATE; GRAIN-SIZE; ORDERED INTERMETALLICS; YIELD STRENGTH; FE-40AL ALLOY; WATER-VAPOR; EMBRITTLEMENT; HYDROGEN; FEAL AB In the present study, the environmental sensitivity under tensile loading of a high-performance Fe-40Al alloy prepared by mechanical alloying is investigated. Testing performed under various environmental conditions reveals a significant loss of ductility in the presence of a moist atmosphere without concomitant change in the stress-strain relationship and fracture mode. Furthermore, this embrittlement is essentially controlled by water vapor. In addition, it is shown that increasing the strain rate improves the final elongation in air up to the intrinsic ductility determined in an inert atmosphere. This embrittlement is attributed to the hydrogen released by the dissociation of water vapor molecules and subsequently swept into the material by mobile dislocations. Tests were designed and carried out specifically to assess the respective roles of exposure duration and straining in this process. Finally, a model is proposed to account for this phenomenon and the mechanisms are discussed. C1 ENSMA, Mecan & Phys Mat Lab, F-86961 Futuroscope Chasseneuil, France. ENSAM, Mecan & Phys Mat Lab, F-49035 Angers, France. RP Henaff, G, ENSMA, Mecan & Phys Mat Lab, F-86961 Futuroscope Chasseneuil, France. TC 4 PD MAR PY 2001 VL 32 IS 3 BP 557 EP 567 UT ISI:000167303000012 ER PT J AU Pessaux, P Burtin, P Arnaud, JP TI Preoperative staging for local extension of rectal carcinoma SO ANNALES DE CHIRURGIE DE endoscopic ultrasonography; preoperative staging of rectal carcinoma; rectal carcinoma ID COMPUTED-TOMOGRAPHY; ENDORECTAL ULTRASOUND; TRANSRECTAL ULTRASOUND; ENDOLUMINAL ULTRASOUND; INTRARECTAL ULTRASOUND; RADIATION-THERAPY; COLORECTAL-CANCER; SURFACE COIL; ULTRASONOGRAPHY; MR AB The treatment of rectal carcinoma is mainly determined by its local extension. Preoperative staging of rectal carcinoma was assessed by different methods: digital rectal examination, transrectal ultrasound, computed tomography, and magnetic resonance imaging. Digital rectal examination had a diagnostic accuracy between 68 and 83 per cent. The accuracy of transrectal ultrasound was between 67 and 93 per cent for tumor staging and between 62 and 88 per cent for lymph node staging. The accuracy of computed tomography was between 33 and 77 per cent for tumor staging and between 22 and 73 per cent for lymph node staging. The overall accuracy of magnetic resonance imaging with body coil was between 59 and 95 per cent, and between 39 and 95 per cent:or lymph node staging. Use of an endorectal coil allows a I:iv more consistent degree of accuracy, with tumor slightly staging accuracy between 66 and 91 per cent, and lymph node staging accuracy between 72 and 79 percent, Preoperative radiation therapy makes transrectal ultrasound and computed tomography less effective as staging techniques. (C) 2001 Editions scientifiques et medicales Elsevier SAS. C1 CHU Angers, Dept Chirurg Viscerale, F-49033 Angers 01, France. CHU Angers, Serv Gastroenterol, F-49033 Angers, France. RP Arnaud, JP, CHU Angers, Dept Chirurg Viscerale, 4 Rue Larrey, F-49033 Angers 01, France. TC 4 PD FEB PY 2001 VL 126 IS 1 BP 10 EP 17 UT ISI:000166844200002 ER PT J AU Haba, D Papon, AP Tanguy, JY Burtin, P Aube, C Caron-Poitreau, C TI Use of half-dose gadolinium-enhanced MRI and magnetization transfer saturation in brain tumors SO EUROPEAN RADIOLOGY DE brain MRI; half-dose gadolinium; meningioma; inter-observer agreement ID ECHO IMAGES; SPIN-ECHO; CONTRAST; LESIONS; TISSUE; DTPA; TIME AB The aim of this study was to search if half-dose gadolinium (Gd)-enhanced MR imaging with magnetization transfer saturation (MT) can replace standard-dose T1-weighted spin echo (SE) without MT saturation in brain tumors. Thirty patients with a total of 33 brain tumors (14 gliomas, 13 meningiomas, 6 metastases) were prospectively studied using T1-weighted SE half-dose of Gd with MT, and T1-weighted SE standard-dose Gd without MT. The contrast-to-noise ratio (CNR) of the two sequences was calculated and four radiologists reviewed qualitatively the images of the two sequences. There was no significant difference between both techniques for quantitative analysis (Wilcoxon test). However, there was a good agreement between sequences to evidence an intraclass correlation coefficient (r = 0.70) of all lesions. In cases of meningioma, the agreement was better (r = 0.84). The results show a difference in the qualitative data between the two sequences, suggesting the use of the T1-weighted MR images with MT and half-dose of Gd with good results in the whole tested parameters except the lesional edema and the presence of artifacts. Half-dose T1-weighted SE with MT can replace standard-dose T1-weighted SE without MT with no loss of contrast enhancement in investigation of meningiomas and saving 50 % of the contrast material. C1 Univ Hosp Angers, Dept Radiol, F-49033 Angers, France. Univ Hosp Angers, Dept Med, F-49033 Angers, France. RP Caron-Poitreau, C, Univ Hosp Angers, Dept Radiol, 4 Rue Larrey, F-49033 Angers, France. TC 4 PY 2001 VL 11 IS 1 BP 117 EP 122 UT ISI:000166064500018 ER PT J AU Subra, JF Renier, G Reboul, P Tollis, F Boivinet, R Schwartz, P Chevailler, A TI Lymphopenia in occupational pulmonary silicosis with or without autoimmune disease SO CLINICAL AND EXPERIMENTAL IMMUNOLOGY DE pulmonary silicosis; autoimmunity; lymphopenia; activated T cells ID PERIPHERAL-BLOOD LYMPHOCYTES; CONNECTIVE-TISSUE DISEASE; INDUCED CELL-DEATH; SILICATE IN-VITRO; T-CELLS; ACTIVATION; EXPOSURE; ANTIBODIES; POPULATIONS; SUBSETS AB An increased prevalence of autoimmune diseases such as rheumatoid arthritis has been demonstrated in silica-exposed patients. The aim of this study was to determine the peripheral blood lymphocyte phenotype in a population of silicotic workers employed in the slate mines of the district. Silicosis was assessed in 58 patients according to the International Labor Office's criteria. Clinical and biological data including flow cytometric evaluation of the lymphocyte subsets were compared with those from 41 healthy volunteers. The silicotic patients had a higher prevalence of autoimmune diseases (6/58 versus 0/41: P < 0.05) and of elevated antinuclear antibody titres compared to the control group. A very significant decrease of total lymphocyte count (P < 0.001) involving B, T and Natural Killer cells was found in silicotic patients as compared with matched healthy volunteers. A significant increase in the percentage of activated T cells (12.3%) was observed in the silicotic group as compared to 6.5% in the control group (P = 5 x 10(-5)). Our results show that in silicotic patients, the absolute number of circulating lymphocytes is diminished with an increased proportion of activated T cells. Whether these findings could predispose to the development of autoimmune disorders is discussed. C1 CHU Angers, Serv Nephrol, F-49033 Angers, France. CHU Angers, Immunol Lab, F-49033 Angers, France. Secur Sociale Miniere, Union Reg Ouest, Trelaze, France. RP Subra, JF, CHU Angers, Serv Nephrol, 4 Rue Larrey, F-49033 Angers, France. TC 3 PD DEC PY 2001 VL 126 IS 3 BP 540 EP 544 UT ISI:000175437200026 ER PT J AU Bonod-Bidaud, C Chevrollier, A Bourasseau, I Lachaux, A de Camaret, BM Stepien, G TI Induction of ANT2 gene expression in liver of patients with mitochondrial DNA depletion SO MITOCHONDRION DE mitochondria; adenine nucleotide translocator; oxidative phosphorylation; mtDNA depletion ID MUSCLE-CELL DIFFERENTIATION; MTDNA DEPLETION; MYOPATHY; FAILURE; DEFICIENCY; MOSAICISM; NUCLEAR AB We have previously described two cases of children with a liver mitochondrial DNA (mtDNA) depletion syndrome, characterised by a low ratio of mtDNA to nuclear DNA. Light microscopy performed on liver biopsy showed abnormal hepatocytes with a characteristic 'oncocytic' appearance, indicative of perturbed oxidative phosphorylation. The adenine nucleotide translocator (ANT), the last step in oxidative phosphorylation catalyses the exchange of adenosine diphosphate (ADP) to adenosine triphosphate (ATP) between the cytosol and mitochondria. The ANT2 gene, which is not normally expressed in human tissues, encodes an isoform preferentially expressed under conditions of glycolytic metabolism: ANT2 gene expression is regulated by a transcription factor involved in a molecular mechanism selecting for the import of glycolytic ATP into the mitochondrial matrix. This ATP import is required in highly proliferative cells, such as tumour cells, which are highly dependent on glycolysis for ATP synthesis. We postulated that, as a result of the defective oxidative phosphorylation observed in these patient biopsies, the ANT2 expression would be induced. We simultaneously quantified the mtDNA depletion and the ANT2 gene expression in liver biopsies from the two patients and six controls. ANT2 mRNA levels were significantly increased in the two patient liver biopsies. Moreover, in one patient, the liver mtDNA depletion was found to be partially reversed after less than 4 years and this reversion was coupled to a concomitant decrease of the ANT2 expression. These results suggest that dysfunction of oxidative phosphorylation could lead to a switch from mitochondrial to glycolytic ATP production, to restore tissue-specific energy requirements. (C) 2001 Elsevier Science B.V. and Mitochondria Research Society. All rights reserved. C1 Univ Angers, Ctr Hosp, Lab Biochim & Biol Mol, F-49033 Angers, France. Univ Lyon 1, CNRS, UMR 5534, Ctr Genet Mol & Cellulaire, F-69622 Villeurbanne, France. Hop Edouard Herriot, Dept Pediat, F-69437 Lyon, France. Hop Debrousse, Lab Biochim Pediat, F-69322 Lyon 05, France. RP Stepien, G, Univ Angers, Ctr Hosp, Lab Biochim & Biol Mol, 4 Rue Larrey, F-49033 Angers, France. TC 3 PD OCT PY 2001 VL 1 IS 3 BP 217 EP 224 UT ISI:000173706000001 ER PT J AU Heer, M Elia, M Ritz, P TI Energy and fluid metabolism in microgravity SO CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE ID DOWN TILT POSITION; SPACE-FLIGHT; BED-REST; BONE-RESORPTION; CIRCADIAN VARIATION; VITAMIN-K; SPACEFLIGHT; TURNOVER; WEIGHTLESSNESS; EXPENDITURE AB Astronauts lose body mass when exploring space. The mechanism was considered to be an upward fluid shift inducing diuresis and natriuresis (the Henry-Gauer mechanism) during the first few days in microgravity. However, recent space missions have shown that neither diuresis nor natriuresis occurs in space missions. In contrast, a sodium-retaining status was shown, which led to positive metabolic sodium balances without fluid retention, Energy intake was consistently reduced whereas energy needs were unchanged, resulting in a negative energy balance of 20%. The reasons for this are many, although proper studies on appetite regulation have not been carried out. Despite numerous countermeasures, bone mass is lost in microgravity situations. These are body composition changes that need to be challenged before humans can be sent to space for extra-long missions. Curr Opin Clin Nutr Metab Care 4:307-311. (C) 2001 Lippincott Williams Wilkins. C1 DLR Inst Aerosp Med, German Aerosp Ctr, D-51170 Cologne, Germany. CHU Angers, Serv Med B, Angers, France. Dunn Clin Nutr Ctr, Cambridge CB2 2DH, England. RP Heer, M, DLR Inst Aerosp Med, German Aerosp Ctr, D-51170 Cologne, Germany. TC 3 PD JUL PY 2001 VL 4 IS 4 BP 307 EP 311 UT ISI:000173720400011 ER PT J AU Eche, N Pichon, MF Quillien, V Gory-Delabaere, G Riedinger, JM Basuyau, JP Daver, A Buecher, B Conroy, T Dieu, L Bidart, JM Deneux, L TI Standards, options and recommendations for tumor markers in colorectal cancer SO BULLETIN DU CANCER DE guidelines; CA 19-9; colorectal neoplasms; tumor markers; carcinoembryonic-antigen ID SERUM CARCINOEMBRYONIC ANTIGEN; POSITRON-EMISSION-TOMOGRAPHY; CROSS-REACTING ANTIGEN; COLON-CARCINOMA-CELLS; IN-SITU HYBRIDIZATION; PLASMA CEA LEVELS; FOLLOW-UP; MONOCLONAL-ANTIBODY; LIVER METASTASES; GASTROINTESTINAL CANCER AB Context: The "Standards, Options and Recommendations " (SOR) project, started in 1993, is a collaboration between the French National Federation of Comprehensive Cancer Centers (FNCLCC), the 20 French Cancer Centers and specialists from French Public University or General Hospital, and Private Clinics. The main objective is the development Of clinical practice guidelines to improve the quality, of health care and outcome of cancer patients, The methodology is based on literature review and critical appraisal by a multidisciplinaty group of experts, with feedback from specialists in cancer care delivery. Objectives: To define, according to the definitions of the Standards, Options and Recommendations project, the characteristics of the main tumor markers in colorectal cancer and their potential role in the management of patients with this malignancy, Methods: Data were identified by searching Medline(R) and the personal reference lists of members of the expert groups. Once the guidelines were defined, the document was submitted for review to 117 independent reviewers, and to the medical committees of the 20 French Cancer Centers. Results: The main recommendations for the tumor markers in colorectal cancer are: 1) The carcinoembryonic antigen (CEA) is the reference serum marker (standard). 2) All the analyses for a given patient must be performed in the same laboratory, using the same technique (standard, expert agreement). 3) CEA or CA 19-9 should not be used for screening or diagnosis (standard level of evidence B2). 4) High initial serum concentration of CEA is or bad predictive value (standard level of evidence Q. CEA is an independent prognostic factor of survival in colorectal cancers with lymph node metastases (standard, level of evidence B2). 5) CEA is the most sensitive biogical parameter for the screening of hepatic metastases (standard, level of evidence B2). 6) CEA serum concentration before palliative chemotheraphy an independent prognostic factor of survival (standard, level of evidence B2). The combination of CEA assay with imagery techniques and clinical examination can help monitor the response to palliative chemotherapy (standard), in particular in non measurable disease (standard expert agreement). 7) In 65% of the cases, CEA is the first indicator of relapse (standard, level of evidence B2). CEA is the choice. level 0 B2). 8) A sustained biological follow-up including CEA assay can be used to predict the operability of recurring tumors (standard, level of evidence B2,). Nevertheless, no survival advantage has been shown (standard). C1 Inst Claudius Regaud, Toulouse, France. Ctr Rene Huguenin, St Cloud, France. Ctr Eugene Marquis, Rennes, France. FNCLCC, Paris, France. Ctr Georges Francois Leclerc, Dijon, France. Ctr Henri Becquerel, F-76038 Rouen, France. Ctr Paul Papin, Angers, France. CHU Nantes, F-44035 Nantes 01, France. Ctr Alexis Vautrin, Vandoeuvre Les Nancy, France. Inst Gustave Roussy, Villejuif, France. Inst Curie, Paris, France. RP Eche, N, Inst Claudius Regaud, Toulouse, France. TC 3 PD DEC PY 2001 VL 88 IS 12 BP 1177 EP 1206 UT ISI:000173374000004 ER PT J AU Coutant, R de Casson, FB Rouleau, S Douay, O Mathieu, E Audran, M Limal, JM TI Body composition, fasting leptin, and sex steroid administration determine GH sensitivity in peripubertal short children SO JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM ID GROWTH-HORMONE GH; IGF-BINDING PROTEIN-3; FACTOR-I; SERUM LEPTIN; REPLACEMENT THERAPY; PREPUBERTAL CHILDREN; DEFICIENT ADULTS; SHORT-TERM; INDIVIDUAL RESPONSIVENESS; POSTMENOPAUSAL WOMEN AB Serum IGF-I levels in GH-treated subjects demonstrate a wide range of responsiveness to GH. However, the factors influencing GH sensitivity are not well known. The aim of this work was 1) to test whether body composition (determined by dual energy x-ray absorptiometry) or factors related to body composition (fasting blood glucose, FFA, C-peptide, leptin, and insulin sensitivity determined by an insulin tolerance test) influence GH sensitivity; and 2) to study the effect of sex steroid priming on GH sensitivity. We measured serum IGF-I at baseline and 24 h after a single administration of GH (2 mg(m(2)) in 60 healthy prepubertal and early pubertal children (height, -2.1 +/- 1.0 SD score). GH sensitivity, as estimated by the increase in serum IGF-I after GH administration (difference between stimulated and baseline serum IGF-I = delta IGF-I), was also determined after a short-term administration of oral ethinyl E2 in girls and im T in boys. The serum IGF-I concentration was 297 +/- 114 mug/liter at baseline and increased to 429 +/- 160 mug/liter, corresponding to a 46.t 29% increase over the baseline value (P < 0.0001, stimulated vs. baseline serum IGF-I). delta IGF-I was not different between gender or pubertal stage. There were positive correlations (P < 0.001) between delta IGF-I and adiposity (total body fat, r = 0.62; trunk fat, r = 0.62), fasting leptin (r = 0.64), and C-peptide (r = 0.54), and a negative correlation with fasting FFA (r = -0.33; P < 0.05) even after adjustment for age, gender, and pubertal stage. These factors remained significant independent predictors of the absolute as well as the percent increase in serum 1GF-I in multiple regression analyses. Priming with T and ethinyl E2 had a similar stimulating effect on the serum GH peak in response to the insulin tolerance test. In boys, serum baseline IGF-I increased by 60%, and delta IGF-I was similar after vs. before T administration. By contrast, in girls, serum baseline IGF-I was similar, and delta IGF-I was 60% less after vs. before ethinyl E2 administration. This study indicates that 1) GH sensitivity is determined by fat mass, serum fasting leptin, C-peptide, and FFA; and 2) oral ethinyl E2 and im T have divergent effects on the IGF-I response to a single administration of GH. C1 Univ Hosp, Dept Pediat, F-49000 Angers, France. Univ Hosp, Dept Nucl Med, F-49000 Angers, France. Univ Hosp, Dept Biochem A, F-49000 Angers, France. Univ Hosp, Dept Biochem B, F-49000 Angers, France. Univ Hosp, Dept Rheumatol, F-49000 Angers, France. RP Coutant, R, Univ Hosp, Dept Pediat, 4 Rue Larrey, F-49000 Angers, France. TC 3 PD DEC PY 2001 VL 86 IS 12 BP 5805 EP 5812 UT ISI:000172728200025 ER PT J AU Leblondel, G Mauras, Y Cailleux, A Allain, P TI Transport measurements across Caco-2 monolayers of different organic and inorganic selenium - Influence of sulfur compounds SO BIOLOGICAL TRACE ELEMENT RESEARCH DE selenium; selenite; selenate; selenocystine; selenomethionine; glutathione; Caco-2 cells ID BRUSH-BORDER MEMBRANE; RAT SMALL-INTESTINE; CELL-LINE CACO-2; IN-VITRO; DRUG ABSORPTION; GLUTATHIONE; MODEL; PERMEABILITY; SELENATE; CYSTEINE AB The transport and uptake of the most common Se compounds, selenate (Seo(4)(2-)), selenite (Seo(3)(2-)), selenomethionine, and selenocystine, were investigated using confluent monolayers of Caco-2 cells, a human carcinoma cell line. Comparative measurements were performed in the absorptive (apical to basolateral side) and exsorptive (basolateral to apical side) directions. Apparent permeability coefficients (P-app), calculated from transport experiments in the absorptive direction, showed increasing values in the following rank order: about 1 x 10(-6) cm/s less than or equal to mannitol less than or equal to SeO32- selenocystine < selenomethionine < SeO42- less than or equal to about 16 x 10(-6) cm/s. The ratios of the P-app measured in the absorptive versus exsorptive directions indicated that only the organic forms presented a net polarized transport (P-app ratio >> 1), suggesting the presence of a transcellular pathway. No significant excretion was observed. The transport of selenomethionine was inhibited by its sulfur analog, methionine, suggesting a common transport mechanism. In contrast, an inhibition of the transport of selenocystine by cysteine was not observed. From the two substrates tested, sulfate and thiosulfate, only thiosulfate inhibited the transport of SeO42-. This effect was also observed for SeO32- (i.e., was unspecific), which questioned the assertion of a common transport for sulfate and SeO42- and may confirm the paracellular pathway of SeO42- suggested by the P-app ratio of about 1. The addition of glutathione (GSH) in large excess had no consequence on the passage of SeO32- but strongly increased the uptake (about fourfold). The liquid chromatography - mass spectrometry (LC-MS) data show ed that, in the ionic condition of incubation medium, GSH promptly reduced SeO32- (less than or equal to 2 min) in its elemental form Se-0, which carrot ascribe to selenodiglutathione a direct role in the effect of GSH. C1 Univ Hosp, Lab Pharmacol & Toxicol, F-49033 Angers 01, France. RP Leblondel, G, Univ Hosp, Lab Pharmacol & Toxicol, 4 Rue Larrey, F-49033 Angers 01, France. TC 3 PD DEC PY 2001 VL 83 IS 3 BP 191 EP 206 UT ISI:000172822800001 ER PT J AU Leblond, H TI Rigorous derivation of the NLS in magnetic films SO JOURNAL OF PHYSICS A-MATHEMATICAL AND GENERAL ID WAVE ENVELOPE SOLITONS; ELECTROMAGNETIC-WAVES; GARNET-FILMS; SPIN-WAVES AB The nonlinear Schrodinger (NLS) equation that gives an account of 'temporal' envelope soliton propagation in magnetic thin films is derived, using the rigorous asymptotic method of multiscale expansions. Magnetostatic backward volume waves are considered and inhomogeneous exchange is neglected. New mathematical features concerning multiscale expansions are found: both a propagating second harmonic term and the usual non-propagating one arise. The secular-type terms that arise in the transverse direction are no longer forbidden. The dispersion coefficient of the obtained NLS equation is not equal to the so-called group velocity dispersion, despite being a general law in bulk media. The nonlinear coefficient is in quite good agreement with the result of previous computations for physically relevant values of the parameters. C1 Univ Angers, UMR CNRS 6136, Lab POMA, F-49045 Angers 01, France. RP Leblond, H, Univ Angers, UMR CNRS 6136, Lab POMA, 2 Bd Lavoisier, F-49045 Angers 01, France. TC 3 PD NOV 16 PY 2001 VL 34 IS 45 BP 9687 EP 9712 UT ISI:000172616600009 ER PT J AU Dobo, I Robillard, N Pineau, D Genevieve, F Piard, N Rapp, MJ Boasson, M Zandecki, M Hermouet, S TI Use of pathology-specific peripheral blood CD34 thresholds to predict leukapheresis CD34 content with optimal accuracy: a bicentric analysis of 299 leukaphereses SO ANNALS OF HEMATOLOGY DE peripheral blood CD34; PBPC; CD34; colony-forming units-granulocyte/macrophage; (CFU-GM); leukapheresis yield; pathology-specific peripheral blood CD34 thresholds ID HEMATOPOIETIC PROGENITOR CELLS; FACTOR G-CSF; CYTOTOXIC CHEMOTHERAPY; PBPC COUNTS; MULTIPLE-MYELOMA; MOBILIZED PBPC; CFU-GM; YIELD; GRANULOCYTE; COLLAGEN AB CD34+ cell counts in peripheral blood (PB) and corresponding numbers of CD34+ cells and colony-forming units-granulocyte/macrophage (CFU-GM) in 299 leukapheresis products of 209 patients undergoing PB progenitor cell (PBPC) mobilization for autologous transplantation in two different centers were analyzed and compared according to diagnosis: non-Hodgkin lymphoma (NHL, 94 leukaphereses), multiple myeloma (MM, 75), Hodgkin's disease (HD, 37), solid tumors (35), and chronic myeloid leukemia (CML, 32). Without separating disease entities, correlations between PB CD34+ cell counts and leukapheresis content of CD34+ cells (r>0.83, P<0.01) and CFU-GM (r>0.81, P<0.01) were excellent. In both centers, a PB CD34 threshold ensuring a leukapheresis yield > 10(6) CD34/kg was determined. This threshold was higher in center I than in center 2, and its predictive accuracy (91.4%, i.e.. prediction correct 91.4% of the time) was significantly lower than in center 2 (98.4%, P=0.02). When data were analyzed by pathology, PB CD34+ cell counts and leukapheresis content of CD34+ cells and CFU-GM remained well correlated, and in both centers PB CD34 thresholds predictive of a yield >10(6) CD34/k-g per leukapheresis could be determined for each pathology. For most patients, pathology-specific PB CD34 thresholds could be obtained directly from the equation of the PB CD34/leukapheresis CD34 correlation curve; they varied depending on both pathology and center (range: 7-20x10(6) CD34/l). Pathology-specific thresholds predicted a leukapheresis yield greater than or equal to 10(6) CD34/kg accurately 100% of the time for MM patients in center 2 and HD and solid tumor patients of both centers, resulting in overall rates of accurate prediction of sufficient graft CD34 content of 96.6% in center 1 and 98.9% in center 2. C1 CHU Nantes, Inst Biol, Hematol Lab, F-44093 Nantes 01, France. CHU Angers, Hematol Lab, Angers, France. Estab Transfus Sanguine Maine & Loire, Angers, France. CHU Nantes, Serv Hematol Clin, F-44093 Nantes, France. CHU Angers, Serv Malad Sang, Angers, France. RP Hermouet, S, CHU Nantes, Inst Biol, Hematol Lab, 9 Quai Moncousu, F-44093 Nantes 01, France. TC 3 PD NOV PY 2001 VL 80 IS 11 BP 639 EP 646 UT ISI:000172518900003 ER PT J AU Fumoleau, P Tubiana-Hulin, M Romieu, G Namer, M Delva, R Guastalla, JP Lotz, JP Kerbrat, P Pujade-Lauraine, E Eichler, F Dohollou, N MacGrogan, G Quinaux, E Grau, B TI A randomized phase II study of 4 or 6 cycles of Adriamycin/Taxol (R) (paclitaxel) as neoadjuvant treatment of breast cancer. SO BREAST CANCER RESEARCH AND TREATMENT C1 Ctr Rene Gauducheau, F-44035 Nantes, France. Ctr Rene Huguenin, St Cloud, France. Ctr Val Aurelle, Montpellier, France. Ctr Antoine Lacassagne, F-06054 Nice, France. Ctr P Papin, Angers, France. Ctr Leon Berard, F-69373 Lyon, France. Hop Tenon, F-75970 Paris, France. Ctr E Marquis, Rennes, France. Hop Hotel Dieu, Paris, France. Hop Civil, Strasbourg, France. Polyclin Nord Aquitaine, Bordeaux, France. Inst Bergonie, Bordeaux, France. IDDI, Brussels, Belgium. Bristol Myers Squibb Co, Paris, France. TC 3 PY 2001 VL 69 IS 3 BP 298 EP 298 UT ISI:000172344800357 ER PT J AU Veal, N Auduberteau, H Lemarie, C Oberti, F Cales, P TI Effects of octreotide on intestinal transit and bacterial translocation in conscious rats with portal hypertension and liver fibrosis SO DIGESTIVE DISEASES AND SCIENCES DE portal hypertension; rats; motility; small intestine; bacterial translocation; portal pressure ID ANALOG SMS 201-995; CIRRHOTIC-PATIENTS; GASTROINTESTINAL MOTILITY; NITRIC-OXIDE; OVERGROWTH; DIMETHYLNITROSAMINE; HEMODYNAMICS; PERITONITIS; FAILURE; MOUTH AB In cirrhosis, delayed intestinal transit may be responsible for increased endoluminal bacterial overgrowth and increased bacterial translocation. Octreotide has been reported to reduce intestinal transit. Therefore, we evaluated whether octreotide administration influences bacterial translocation in a model of liver fibrosis secondary to dimethylnitrosamine (DMNA) administration. Twenty-nine conscious rats were randomly assigned to three groups (sham rats + placebo as controls, DMNA + placebo, DMNA + octreotide, 1.5 mug/kg thrice daily subcutaneously), and including portal pressure, intestinal transit (radioactive method), and bacterial translocation were measured. Three of four variables measuring intestinal transit suggested a significant delay in intestinal transit in DMNA rats compared to controls (eg, cumulated radioactivity 50%: controls: 5.3 +/- 1.5, DMNA + placebo: 3.2 +/- 1.2, DMNA + octreotide: 2.7 +/- 1.9, P < 0.01). This delay tended to be enhanced by octreotide but the effect was only significant with one of the intestinal transit variables. Bacterial translocation was significantly increased in DMNA rats compared to controls but octreotide did not increase translocation [eg, germ count (log) in lymph nodes: controls: 3.1 +/- 3.6, DMNA + placebo: 12.3 +/- 4.4, DMNA + octreotide: 10.6 +/- 6.0, P < 0.001]. There was no significant correlation of portal pressure, intestinal transit, and bacterial translocation in this study. In conclusion, our results show that, although octreotide worsens delayed intestinal transit, it has no influence on the level of bacterial translocation. C1 Univ Angers, Lab HIFIH, UPRES EA 2170, Angers, France. CHU Angers, Bacteriol Lab, Angers, France. RP Cales, P, CHU Angers, Serv Hepatogastroenterol, F-49033 Angers 01, France. TC 3 PD NOV PY 2001 VL 46 IS 11 BP 2367 EP 2373 UT ISI:000172109300012 ER PT J AU Paramelle, O Croue, A Dupre, F Rialland, X Saint-Andre, JP TI Pelvic malignant ectomesenchymoma: a case report SO ANNALES DE PATHOLOGIE DE ectomesenchymoma; gangliorhabdomyosarcoma; rhabdomyoblast; neuroblast; ganglion cell; immunohistochemistry ID DIFFERENTIATION; TUMORS; CELL AB We report the case of a 19 month old boy referred to our institution because of a pelvic tumor initially identified as an embryonal rhabdomyosarcoma and treated with surgery and chemotherapy. Eight years after the initial surgery, a local tumor recurrence with bone metastasis was found, Histological examination and immunohistochemistry showed a double differentiation with both muscular and neuronal cells, This double differentiation was retrospectively found in the initial tumor, then allowing the diagnosis of malignant ectomesenchymoma also called gangliorhabdomyosarcoma. This rare tumor, occurring mainly during childhood, is composed of neuroblasts and/or ganglion cells and of malignant mesenchymal cells (usually rhabdomyosurcomatous cells). C1 CHU Angers, Anat Pathol Lab, F-49033 Angers 01, France. CHU Angers, Dept Pediat Med, F-49033 Angers, France. RP Paramelle, O, CHU Angers, Anat Pathol Lab, F-49033 Angers 01, France. TC 3 PD AUG PY 2001 VL 21 IS 4 BP 344 EP 347 UT ISI:000172013600008 ER PT J AU Mahe, D Fisson, S Montoni, A Morel, A Couez, D TI Identification and IFN gamma-regulation of differentially expressed mRNAs in murine microglial and CNS-associated macrophage subpopulations SO MOLECULAR AND CELLULAR NEUROSCIENCE ID CENTRAL-NERVOUS-SYSTEM; NITRIC-OXIDE SYNTHASE; MYELIN BASIC-PROTEIN; CD8(+) T-CELLS; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; ANTIGEN-PRESENTING CELLS; MESSENGER-RNA; RAMIFIED MICROGLIA; P-SELECTIN; KERATAN SULFATE AB CNS-resident macrophages (microglia and CNS-associated macrophages) are the main immunocompetent cells of the central nervous system (CNS) and respond by rapid activation to brain injury. Molecular events occurring during IFN gamma -activation and identification of potential markers of the CNS-resident macrophage subsets were investigated using microglial-derived clones (EOC) differing in their morphology and their antigen presenting activities for CD4(+) and CD8(+) T-cells. By applying the subtractive process of cDNA representational difference analysis (cRDA), 16 differentially expressed mRNAs were isolated and sequenced, revealing 8 known and 8 novel molecules; 15 of these messages were unpreviously reported in microglia. Two markers of all activated microglial EOC cells were identified (iNOS; IRG-1) and specific subpopulation markers were highlighted, including molecules known to be closely expressed in perivascular spaces. Moreover, some messages could support the distinct morphology, adhesive characteristics, and potential functions of the different clones. C1 CHU Angers, Lab Biol Mol Immunol & Therapeut Cancers, BMITC, UPRES EA 3140, F-49033 Angers 01, France. RP Couez, D, CHU Angers, Lab Biol Mol Immunol & Therapeut Cancers, BMITC, UPRES EA 3140, Batiment Monteclair,4 Rue Larrey, F-49033 Angers 01, France. TC 3 PD OCT PY 2001 VL 18 IS 4 BP 363 EP 380 UT ISI:000171885400003 ER PT J AU Heyman, D Delhaye, M Fournier, D Mercier, P Rousselet, MC Menei, P TI Pseudotumoral demyelination: a diagnosis pitfall (report of three cases) SO JOURNAL OF NEURO-ONCOLOGY DE demyelinating disease; brain tumor; central nervous system; brain MRI; brain biopsy; pitfall ID BALO CONCENTRIC SCLEROSIS; MULTIPLE-SCLEROSIS; CORPUS-CALLOSUM; DISEASE; GLIOMA; TUMOR AB Rare forms of demyelinating disease such as Balo's concentric sclerosis or Schilder's disease may simulate brain tumors, both clinically and on the computed tomography (CT) and magnetic resonance imaging (MRI). Even the histopathological diagnosis after a biopsy is not entirely reliable. We report three cases of pseudotumoral demyelinating disorders having required a stereotaxic biopsy, one of which was erroneously diagnosed as a malignant astrocytoma. We describe MRI especially the intense contrast enhancement with ill-defined margins, and the mild mass effect. We then detail the histopathological processes upon which differential diagnosis with a tumor can be based. C1 Univ Hosp, Dept Neurosurg, Angers, France. Univ Hosp, Pathol Lab, Angers, France. Sch Med, Lab Anat, Angers, France. RP Menei, P, Hop Larrey, Serv Neurochirurg, 4 Rue Larrey, F-49033 Angers, France. TC 3 PD AUG PY 2001 VL 54 IS 1 BP 71 EP 76 UT ISI:000171945300010 ER PT J AU Bellard, E Fortrat, JO Vielle, B Dupuis, JM Victor, J Leftheriotis, G TI Early predictive indexes of head-up tilt table testing outcomes utilizing heart rate and arterial pressure changes SO AMERICAN JOURNAL OF CARDIOLOGY ID NEURALLY-MEDIATED SYNCOPE; UNEXPLAINED SYNCOPE; REPRODUCIBILITY AB The 45-minute head-upright tilt test (HUTT) at an angle of 60 degrees to 70 degrees (i.e., Westminster protocol) is a standard protocol for the diagnosis of unexplained syncope(1,2) has acceptable sensitivity and specifleity.(4) A major drawback is its long duration, especially for the elderly and patients disabled by traumatic falls. Early predictive indexes of HUTT outcome based on heart rate (HR) changes during the test have been reported by Mallat et al,(5) and more recently by Alvarez et al.(6) These previous observations were based upon the analysis of early (6 and 5 minutes, respectively) responses in HR and yielded contradictory findings. The purpose of the present study was to determine the potential usefulness of early changes in arterial pressure in combination with HR as predictors of the outcome of passive HUTT. C1 Med Sch Angers, Physiol Lab, UPRES EA 2170, F-49035 Angers, France. Univ Teaching Hosp Angers, Dept Stat, Angers, France. Univ Teaching Hosp Angers, Dept Cardiol, Angers, France. RP Leftheriotis, G, Med Sch Angers, Physiol Lab, UPRES EA 2170, Rue Haute Reculee, F-49035 Angers, France. TC 3 PD OCT 15 PY 2001 VL 88 IS 8 BP 903 EP + UT ISI:000171635200019 ER PT J AU Pessaux, P Tuech, JJ Arnaud, JP TI Hemorroidectomy by circular anopexia: the Longo technique SO JOURNAL DE CHIRURGIE C1 CHU Angers, Serv Chirurg Viscerale, F-49033 Angers 01, France. RP Arnaud, JP, CHU Angers, Serv Chirurg Viscerale, F-49033 Angers 01, France. TC 3 PD AUG PY 2001 VL 138 IS 4 BP 222 EP 225 UT ISI:000171297700006 ER PT J AU Roche, PH Fournier, HD Laccourreye, L Mercier, P TI Surgical anatomy of the infratemporal fossa using the transmaxillary approach SO SURGICAL AND RADIOLOGIC ANATOMY DE cranial base surgery; infratemporal fossa; maxillary sinus; pterygopalatine fossa; surgical anatomy ID MICROSURGICAL ANATOMY; CRANIAL BASE; TUMORS AB In this study we evaluated the ability of the transmaxillary route to expose the elements of the infratemporal fossa (ITF). Five adult cadaver heads were dissected on both sides, after making a paralateronasal incision. The maxillary branch of the trigeminal nerve served as a superior landmark to progress into the retroantral space and pterygopalatine fossa. The maxillary artery, lateral pterygoid muscle, pterygoid venous plexus, foramen rotundum and foramen ovale were identified. Distances between those elements and angle of approaches of the foramen ovale and foramen rotundum were measured in the horizontal plane. In all cases, the anterior loop of the maxillary artery and the sphenopalatine artery were located in the proximal retroantral fatty space and could be ligated without optic magnification. The maxillary nerve could be followed up to the foramen rotundum at a 44 mm mean distance from the opening. The mean angle of vision to the foramen rotundum was 31 degrees. Under the greater sphenoid wing and lateral to the pterygoid process, desinsertion and partial resection of the lateral pterygoid muscle were required to identify the pterygoid venous plexus and foramen ovale. The pterygoid venous plexus was organized as a compact network of channels between and superior to the muscle fibers; it was in close relation with the foramen ovale. Access to the foramen ovale was deep (mean 56 mm) and narrow (20 degrees). Our results indicate that the transmaxillary approach is a minimally invasive procedure that gives an appropriate window to the structures of the retroantral space and to the pterygomaxillary fissure and pterygopalatine fossa. Monitoring of the retropterygoid portion of the infratemporal fossa by this route is inadequate. C1 Fac Med, Lab Anat, F-49045 Angers, France. RP Mercier, P, Fac Med, Lab Anat, Rue Haute de Reculee, F-49045 Angers, France. TC 3 PD AUG PY 2001 VL 23 IS 4 BP 209 EP 213 UT ISI:000171246400001 ER PT J AU Tonneau, A Gerland, M Henaff, G TI Environment-sensitive fracture of iron aluminides during cyclic crack growth SO METALLURGICAL AND MATERIALS TRANSACTIONS A-PHYSICAL METALLURGY AND MATERIALS SCIENCE ID TIAL-BASED ALLOY; FE3AL ALLOYS; FATIGUE; PROPAGATION; EMBRITTLEMENT AB The present study investigates the fatigue-crack propagation resistance of an FeAl-based alloy, with a special emphasis placed on the analysis of the mechanisms involved in environmentally assisted crack growth. To this end, a series of tests have been conducted at room temperature in air, in high and low vacuum, and in argon. The results reveal a significant fatigue-crack growth (FCG) enhancement when the material is fatigued in a moist environment. This enhancement may, however, be inhibited by oxygen in the case of a low water-vapor partial pressure. Besides, the analysis of the results obtained under various exposure conditions suggests that the FCG enhancement in air is mostly controlled by water-vapor adsorption, which reduces the energy required to create a unit cracked area. Examination of dislocation structures within the plastic zone reveals a lower extent of plasticity in air, consistent with this mechanism. Predictions obtained by a model of adsorption-assisted propagation provide an additional support for the adsorption assumption. Finally, an FCG growth law is proposed to describe the intrinsic resistance as observed in inert environments and to account for the mechanism operative in fatigue-crack propagation in ambient air. C1 ENSAM, Lab Procedes Mat Instrumentat, F-49000 Angers, France. Mecan & Phys Mat Lab, F-86961 Futuroscope Chasseneuil, France. RP Tonneau, A, ENSAM, Lab Procedes Mat Instrumentat, BP325, F-49000 Angers, France. TC 3 PD SEP PY 2001 VL 32 IS 9 BP 2345 EP 2356 UT ISI:000170925100018 ER PT J AU Plantier, G Servagent, N Sourice, A Bosch, T TI Real-time parametric estimation of velocity using optical feedback interferometry SO IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT DE optical feedback; optical sensor; velocity measurement; spectral analysis; multiplicative noise; signal processing AB A low-cost laser sensor using optical feedback interferometry has been designed to measure velocities. With digital signal processing based on an order two autoregressive model of the optical power, an inaccuracy of about 0.5% can be reached. C1 Ecole Super Elect Ouest, Angers 01, France. Ecole Mines Nantes, Dept Automat Control & Prod Syst, Nantes 3, France. RP Plantier, G, Ecole Super Elect Ouest, Angers 01, France. TC 3 PD AUG PY 2001 VL 50 IS 4 BP 915 EP 919 UT ISI:000170885300013 ER PT J AU Tuech, JJ Pessaux, P Regenet, N Bergamaschi, R Arnaud, JP TI Emergency pancreaticoduodenectomy with delayed reconstruction for bleeding - A life saving procedure SO INTERNATIONAL JOURNAL OF PANCREATOLOGY DE pancreaticoduodenectomy; emergency; reconstruction; delayed; damage control AB Emergency pancreaticoduodenectomy with delayed reconstruction can be performed as a life saving procedure in case of massive bleeding uncontrolled with conventional hemostatic techniques. The authors report herein the case of a 39-yr-old patient with an acute episode of chronic pancreatitis-induced massive bleeding successfully treated by this unorthodox technique. The concept of damage control surgery with abbreviated laparotomy and planned reconstruction could be useful in selected cases outside the trauma setting. C1 Angers Univ Hosp, Dept Digest Surg, F-49000 Angers, France. RP Tuech, JJ, Angers Univ Hosp, Dept Digest Surg, 4 Rue Larrey, F-49000 Angers, France. TC 3 PY 2001 VL 29 IS 1 BP 59 EP 62 UT ISI:000170750400009 ER PT J AU Allain, P Le Gall, D Etcharry-Bouyx, F Forgeau, M Mercier, P Emile, J TI Influence of centrality and distinctiveness of actions on script sorting and ordering in patients with frontal lobe lesions SO JOURNAL OF CLINICAL AND EXPERIMENTAL NEUROPSYCHOLOGY ID KNOWLEDGE; MEMORY; REPRESENTATION; DAMAGE AB In order to further investigate script knowledge in patients with frontal lobe lesions (FP), 30 participants with frontal brain damage, 14 controls with posterior brain damage (PC) and 30 normal controls (NC) were asked to sort script actions with various degrees of centrality and distinctiveness according to the script to which they belonged and their order of execution. Actions were given in 3 conditions: scripts with headers (SH), scripts without headers (SwH) and scripts with distractor header (SDH). Results confirmed the dual dissociation we observed in a previous study (Allain, Le Gall, Etcharry-Bouyx, Aubin, & Emile, 1999). Twelve FP made numerous errors in sequencing actions regardless of the conditions, but rejected the irrelevant title. Fourteen FP made few sequencing errors but used the irrelevant title. The last 4 FP performed as well as the PC and NC in every condition. Sorting errors made by the FP mainly concerned actions with low levels of centrality and distinctiveness. These data are consistent with the view that frontal lobe lesions selectively impair the syntactic component of script representation without affecting its semantic component. C1 Univ Angers, Psychol Lab, Dept Psychol, UPRES EA 2646, F-49033 Angers 01, France. Univ Hosp Angers, Dept Neurosurg, F-49033 Angers, France. Univ Hosp Angers, Dept Neurol, Neuropsychol Unit, Angers, France. RP Allain, P, CHU Angers, Cent Hosp, Unite Neuropsychol,Sec Charcot, Dept Neurol, 4 Rue Larrey, F-49033 Angers 01, France. TC 3 PD AUG PY 2001 VL 23 IS 4 BP 465 EP 483 UT ISI:000170566500005 ER PT J AU Bensmaine, MA Marty, M de Gramont, A Brienza, S Levi, F Ducreux, M Francois, E Gamelin, E Bleiberg, H Cvitkovic, E TI Factors predicting efficacy of oxaliplatin in combination with 5-fluorouracil (5-FU) +/- folinic acid in a compassionate-use cohort of 481 5-FU-resistant advanced colorectal cancer patients SO BRITISH JOURNAL OF CANCER DE clinical resistance; multivariate analysis; salvage chemotherapy ID FIRST-LINE TREATMENT; CONTINUOUS-INFUSION 5-FLUOROURACIL; MULTICENTER RANDOMIZED TRIAL; HIGH-DOSE LEUCOVORIN; PHASE-II; FLUOROURACIL; CHEMOTHERAPY; IRINOTECAN; CARCINOMA; CISPLATIN AB A statistical analysis was performed on the patient data collected from two compassionate-use programmes using oxaliplatin (Eloxatin (R)) + 5-fluorouracil (5-FU) +/- folinic acid (FA), to identify predictive factors for oxaliplatin-based salvage treatment in patients with 5-FU-resistant advanced colorectal cancer (ACRC). 481 5-FU-resistant ACRC patients, most with performance status less than or equal to 2, greater than or equal to 3 involved sites, and greater than or equal to 2 prior lines of chemotherapy, received oxaliplatin + 5-FU +/- FA. Prognostic factors associated with overall response rate (ORR), time to progression (TTP) and overall survival (OS) were identified using univariate and multivariate logistic and/or Cox proportional hazards analyses. The ORR was 16% (95% Cl: 13-20), the median TTP was 4.2 months (95% Cl: 3.4-4.6), and the median OS was 9.6 months (95% Cl: 8.6-10.6). The multivariate analysis indicated poor (greater than or equal to 2 WHO) performance status (PS), a large number of prior chemotherapy regimens (greater than or equal to 3), a low baseline haemoglobin level (< 10 g/dl), and a triweekly (vs biweekly) treatment administration schedule as significantly associated (P < 0.05) with a lower ORR. Sex (male), number of organs involved (greater than or equal to3) and alkaline phosphatase (AP) level (greater than or equal to 2 x the upper limit of normal) were associated (P < 0.05) with shorter TTP. Poor PS, a large number of organs involved, and elevated AP were independently and significantly correlated with shorter OS. Our analysis identified a relationship between efficacy results of oxaliplatin + 5-FU FA treatment in 5-FU-resistant ACRC patients and baseline prognostic factors related to PS, extent of disease and number of prior regimens. (C) 2001 Cancer Research Campaign. C1 Cvitkovic & Associes Consultants, F-94278 Kremlin Bicetre, France. Hop St Louis, F-75010 Paris, France. Hop St Antoine, F-75012 Paris, France. Debioclin, F-94220 Charenton Le Pont, France. Hop Paul Brousse, F-94805 Villejuif, France. Inst Gustave Roussy, F-94805 Villejuif, France. Ctr Lacassagne, F-06050 Nice 01, France. Ctr Paul Papin, F-49033 Angers 01, France. Inst Jules Bordet, B-1000 Brussels, Belgium. RP Cvitkovic, E, Cvitkovic & Associes Consultants, 18-20 Rue Pasteur, F-94278 Kremlin Bicetre, France. TC 3 PD AUG 17 PY 2001 VL 85 IS 4 BP 509 EP 517 UT ISI:000170626900007 ER PT J AU Mouthon, L Khaled, M Cohen, P Guillevin, L Mouthon, L Subra, JF TI Systemic small sized vessel vasculitis after massive antigen inhalation SO ANNALS OF THE RHEUMATIC DISEASES ID CHURG-STRAUSS-SYNDROME; WEGENERS GRANULOMATOSIS; FOLLOW-UP; ANGIITIS C1 Univ Paris 13, Serv Med Interne, Hop Avicenne, F-93009 Bobigny, France. SMBH Leonard de Vinci, UFR, UPRES EA 2361, Bobigny, France. CHU Angers, Serv Nephrol, Angers, France. RP Mouthon, L, Univ Paris 13, Serv Med Interne, Hop Avicenne, 125 Route Stalingrad, F-93009 Bobigny, France. TC 3 PD SEP PY 2001 VL 60 IS 9 BP 903 EP 904 UT ISI:000170612700026 ER PT J AU Mikhalevich, V Debenay, JP TI The main morphological trends in the development of the foraminiferal aperture and their taxonomic significance SO JOURNAL OF MICROPALAEONTOLOGY ID REVISION AB As a result of the intensive movement of the cytoplasm through the aperture when communication with the environment is required, this area has an important and variable functional burden. Additional skeletal structures have a fundamental supporting function along the Course of this strong cytoplasmic stream and may be related to the compartmentalization and differentiation of the cytoplasm. As a result of these important functional roles, the structure of the aperture is one of the basic diagnostic features in foraminiferal taxonomy. The simplest and least diverse apertural types are found in the most ancient unilocular or pseudo-chambered representatives of the classes Lagynata and Astrorhizata. Their development from simple to complicated ones in the different classes (following the new foraminiferal macrosystem proposed by Mikhalevich) shows a significant number of parallelisms and convergences. In both the lower agglutinated groups and the higher calcareous members of the classes Spirillinata, Miliolata, Nodosariata and Rotaliata, the evolutionary trends of the apertures are similar within the same class, even if those of the lower groups are always structurally simpler and less diversified. These trends continue until all the possibilities of the pre-existing structures are exhausted. Then, new structures, sometimes affecting the whole cell organization arise and the possibilities of morphological changes multiply, leading to evolutionary divergence. C1 RAS, Zool Inst, Dept Protozool, St Petersburg 199034, Russia. Fac Sci, UPRES EA 2644, Geol Lab, F-49045 Angers, France. RP Mikhalevich, V, RAS, Zool Inst, Dept Protozool, St Petersburg 199034, Russia. TC 3 PD JUL PY 2001 VL 20 PN Part 1 BP 13 EP 28 UT ISI:000170272700003 ER PT J AU Trebaol, G Manceau, C Tirilly, Y Boury, S TI Assessment of the genetic diversity among strains of Xanthomonas cynarae by randomly amplified polymorphic DNA analysis and development of specific characterized amplified regions for the rapid identification of X-cynarae SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY ID POLYMERASE-CHAIN-REACTION; PSEUDOMONAS-SYRINGAE; XYLELLA-FASTIDIOSA; PCR PRIMERS; CAMPESTRIS; FRAGMENTS; AMPLIFICATION; LISTERIA; PATHOGEN; MARKERS AB The randomly amplified polymorphic DNA (RAPD) method was used to investigate the genetic diversity in Xanthomonas cynarae, which causes bacterial bract spot disease of artichoke. This RAPD analysis was also intended to identify molecular markers characteristic of this species, in order to develop PCR-based markers which can be used to detect this pathogenic bacterium in artichoke fields. Among the 340 RAPD primers tested, 40 were selected on their ability to produce reproducible and reliable fingerprints in our genetic background. These 40 primers produced almost similar patterns for the 37 X. cynarae strains studied, different from the fingerprints obtained for other Xanthomonas species and other xanthomonad-like bacteria isolated from artichoke leaves. Therefore, X. cynarae strains form a homogeneous genetic group. However, a little DNA polymorphism within this species was observed and the collection of X. cynarae isolates was divided into two groups (one containing three strains, the second one including all other strains). Out of seven RAPD markers characteristic of X. cynarae that were cloned, four did not hybridize to the genomic DNA of strains belonging to other Xanthomonas species. These four RAPD markers were converted into PCR markers (specific characterized amplified regions [SCARs]); they were sequenced, and a PCR primer pair was designed for each of them. Three derived SCARs are good candidates to develop PCR-based tests to detect X. cynarae in artichoke fields. C1 BBV, Penn Ar Prat, F-29250 St Pol de Leon, France. Univ Angers, INH, INRA, UMR Pave, F-49071 Beaucouze, France. ISAMOR, ESMISAB, Technopole Brest Iroise, F-29280 Plouzane, France. RP Boury, S, BBV, Penn Ar Prat, F-29250 St Pol de Leon, France. TC 3 PD AUG PY 2001 VL 67 IS 8 BP 3379 EP 3384 UT ISI:000170297800007 ER PT J AU Lode, T TI Genetic divergence without spatial isolation in polecat Mustela putorius populations SO JOURNAL OF EVOLUTIONARY BIOLOGY DE endogamy; genetic divergence; habitat preferences; Mustela; polymorphism; sympatric differentiation ID SYMPATRIC SPECIATION; WESTERN FRANCE; PUMPKINSEED SUNFISH; ALLOZYME VARIATION; EUROPEAN POLECAT; NORTH-AMERICA; ARCTIC CHARR; SELECTION; DIFFERENTIATION; HETEROZYGOSITY AB Understanding how genetic divergence could exist without spatial isolation is a fundamental issue in biology. Although carnivores have previously been considered as having a weak genetic variability, polecats Mustela putorius from eight distinct populations exhibited both a strong polymorphism (17.5-22.5%) and a substantial allele effective number reaching N-e = 1.12. Heterozygosity ranging from H-o = 0.031-0.063 significantly differed among populations, while the mean F-IS averaging 0.388 stressed a real deficiency of heterozygotes. Observed heterozygosity levels among populations did not correlate with any habitat types but were clearly associated with habitat diversity index. The habitat structure in polecat home range corresponded to habitat mosaic structure in which discrete habitat types alternated causing multifactorial constraints that may favour heterozygosity. Allozymic frequencies within populations did not vary with dominant habitat. But in the Tyrosinase-1, the rare homozygote BB, resulting in a 'dark' phenotype, was found much more in deciduous woods than the homozygote AA showing the 'typical' pattern. Thus, the genetic basis for a character differentiation was here evidenced in a remarkable situation without spatial isolation. Further, the very low proportion of heterozygotes for this locus suggests a disruptive effect and supports the prediction of intermediate phenotypes being at a disadvantage. This heterozygote deficit map also result from an assortative mating intra phenotype (homogamy). The divergence in polecat phenotypes showed that genetic differentiation can be induced by subtle variations in environment, a situation that is likely to be frequent in most natural populations, and emphasized the adaptive nature of habitat preference. C1 Univ Angers, Fac Sci, Lab Ecol Anim, F-49045 Angers, France. RP Lode, T, Univ Angers, Fac Sci, Lab Ecol Anim, F-49045 Angers, France. TC 3 PD MAR PY 2001 VL 14 IS 2 BP 228 EP 236 UT ISI:000169755900004 ER PT J AU Ferrari, L De Sevin, F Vigue, JP Granry, JC Preckel, MP TI Intracranial subdural haematoma after dural puncture in obstetric SO ANNALES FRANCAISES D ANESTHESIE ET DE REANIMATION DE blood patch; dural puncture; epidural anaesthesia; headache; subdural haematoma ID SPINAL-ANESTHESIA; EPIDURAL ANALGESIA; HEMATOMA; HEADACHE; COMPLICATION AB The authors report a case of subdural haematoma (HSD), which occurred following epidural analgesia for labour, complicated by post dural puncture headache (PDPH). A 26-year-old woman displayed a typical PDPH following epidural anaesthesia. On the sixth day, she was given a blood patch (BP), which was partially efficacious. Because of worsening of the headache, of disappearance of the postural characteristics, and of vomiting without focal neurological signs on the 9(th) day, a CT-scan was done. The CT-scan showed a small hemispheric subdural haematoma. The recovery was complete with only medical treatment. HSD is a rare serious complication of dural puncture. When the characteristics of PDPH change, HSD should be evoked even without focal neurological signs. An early diagnosis and the small size of the haematoma may allow HSD to be treated medically and avoid surgical evacuation. (C) 2001 Editions scientifiques et medicales Elsevier SAS. C1 CHU Angers, Dept Anesthesie Reanimat, F-49033 Angers 01, France. RP Ferrari, L, CHU Angers, Dept Anesthesie Reanimat, F-49033 Angers 01, France. TC 3 PD JUN PY 2001 VL 20 IS 6 BP 563 EP 566 UT ISI:000169789300010 ER PT J AU Mouthon, L Khaled, M Cohen, P Subra, JF Guillevin, L TI Antigen inhalation as a triggering factor in systemic small-sized-vessel vasculitis - Four cases SO ANNALES DE MEDECINE INTERNE DE vasculitis; Wegener's granulomatosis; Churg-Strauss syndrome; antigen; inhalation ID CHURG-STRAUSS-SYNDROME; WEGENERS-GRANULOMATOSIS; POLYARTERITIS-NODOSA; ARTHUS REACTION; FC-RECEPTORS; FOLLOW-UP; ANGIITIS; ZAFIRLUKAST; THERAPY AB In order to identify small-sized-vessel vasculitis occurring immediately after massive inhalation of particles which could be considered antigenic, we performed a retrospective review of patients who consulted our Department of Internal Medicine between 1980 and 1998 and were diagnosed as having small-vessel vasculitis that developed immediately after massive exposure to inhaled particles. Four patients, 27 to 55 years old, presented with small-sized-vessel vasculitis tone with Wegener's granulomatosis and three with Churg-Strauss syndrome) which developed after massive inhalation of particles, Clinical manifestations occurred from a few hours to 10 days after inhalation of diesel fumes, cereal dust, flour or cereal dust and/or pigeon droppings, Three patients remain disease-free 8, 9 and 18 years after discontinuing treatment, One patient remains well after 3 years on low-dose corticosteroids and cyclophosphamide therapy. Our data suggest that primary antigens can cause small-sized-vessel vasculitides. Identifying such etiological factors could improve our understanding of the pathogenesis of systemic vasculitides and prevent relapses due to similar antigenic exposure. C1 Univ Paris 13, Hop Avicenne, Serv Med Interne, F-93009 Bobigny, France. CHU Angers, Serv Nephrol, Angers, France. RP Mouthon, L, Univ Paris 13, Hop Avicenne, Serv Med Interne, 125 Route Stalingrad, F-93009 Bobigny, France. TC 3 PD APR PY 2001 VL 152 IS 3 BP 152 EP 156 UT ISI:000169716900002 ER PT J AU Abgueguen, P Chennebault, JM Achard, J Cottin, J Pichard, E TI Cat scratch disease. Clinical study of 26 patients. Place and importance of PCR. SO REVUE DE MEDECINE INTERNE DE cat scratch disease; serology; histology; polymerase chain reaction; Bartonella henselae ID BARTONELLA-HENSELAE; ROCHALIMAEA-HENSELAE; INFECTION; QUINTANA; AZITHROMYCIN; VINSONII; THERAPY; NOV AB Purpose. - Cat scratch disease is a mild pathology but diagnosis often remain difficult. Methods. - A retrospective study has been conducted by the department of infectious diseases at the University hospital in Angers. Between January 1994 and October 1998, 26 observations were recorded providing the presence of three criteria out of four among the following: contact with a cat, clinical presentation and its favorable course, absence of any other cause noticed, and the presence of either a positive serology or a positive PCR, or the examination of a suggestive pathology. Results. - Fourteen men and 12 women were concerned. From a clinical point of view, the inoculation lesion was observed six times, all patients showed at least one adenopathy during their illness, 12 patients showed only an adenopathy without clinical signs. A surgical biopsy was carried out on nine patients and a diagnosis established. Bartonella henselae serology was done in all patients. Six presented a significant rate of IgG antibodies as early as the first dosage. A seroconversion was observed in four cases belatedly 1 to 2 months after the beginning of the symptomatology. The method's sensitivity was approximately 38%. A PCR search was accomplished in the pus obtained from a ganglionic puncture on 12 patients. It was positive seven times, which corresponds to a sensitivity of about 58%. In associating these two diagnostic criteria a sensitivity rate of nearly 92% was reached, the diagnosis not having been confirmed only in one case. The outcome proved to be favorable in all cases, with or without an antibiotic treatment. Conclusion. - The association of serology and PCR in the pus permits a certain diagnosis in the majority of the cases and avoids the more aggressive biopsy. (C) 2001 Editions scientifiques et medicales Elsevier SAS. C1 CHU Angers, Serv Malad Infect, F-49033 Angers 01, France. RP Abgueguen, P, CHU Angers, Serv Malad Infect, 4 Rue Larrey, F-49033 Angers 01, France. TC 3 PD JUN PY 2001 VL 22 IS 6 BP 522 EP 529 UT ISI:000169396900006 ER PT J AU Siepmann, J Peppas, NA TI Mathematical modeling of controlled drug delivery SO ADVANCED DRUG DELIVERY REVIEWS C1 Univ Angers, Coll Pharm, F-49100 Angers, France. Purdue Univ, Sch Chem Engn, W Lafayette, IN 47907 USA. RP Siepmann, J, Univ Angers, Coll Pharm, 16 Blvd Daviers, F-49100 Angers, France. TC 3 PD JUN 11 PY 2001 VL 48 IS 2-3 BP 137 EP 138 UT ISI:000169091200001 ER PT J AU Bourgeais, AM Avenel-Audran, M Le Bouil, A Bouyx, C Allain, P Verret, JL TI Chronic arsenicism SO ANNALES DE DERMATOLOGIE ET DE VENEREOLOGIE ID SKIN-CANCER; DRINKING-WATER; DISEASE; HAIR AB Background. Arsenic is an ubiquitous natural element. Chronic and low level ingestion or inhalation may result in chronic arsenicism first characterized by skin changes. Case report. A 75 year old man, non-insulin-dependent diabetic, presented a diffuse hyperpigmentation with scattered white spots on the trunk. He complained of asthenia. Clinical diagnosis of chronic arsenicism was confirmed by arsenic determination in urine, plasma and phaneres. Thorough investigations led to discover very high arsenic levels in the own wine of the patient. This was probably the result of a wrong use of sodium arsenite-based fungicide, for cultivating his vi ne yard. Discussion. Chronic arsenicism has become rare but it should always be kept in mind. Clinical presentation, with particular cutaneous features and routes of exposure are reviewed. Treatment is symptomatic. As arsenic is known to be a strong carcinogenic agent, patients with chronic arsenicism have to be followed up during a long time. C1 CHU Angers, Serv Dermatol & Venerol, F-49033 Angers 01, France. CHU Angers, Lab Pharmacol & Toxicol, F-49033 Angers, France. RP Verret, JL, CHU Angers, Serv Dermatol & Venerol, 4 Rue Larrey, F-49033 Angers 01, France. TC 3 PD APR PY 2001 VL 128 IS 4 BP 527 EP 530 UT ISI:000168847100007 ER PT J AU Guiot, E Benayoun, S Nouet, G Djouadi, M Masri, P Lambertin, M TI Formation and growth of c-BN films in various conditions: improvement of the adherence SO DIAMOND AND RELATED MATERIALS DE adhesion; cubic boron nitride; nucleation; physical vapor deposition ID CUBIC BORON-NITRIDE; BEAM-ASSISTED DEPOSITION; PULSED-LASER DEPOSITION; THIN-FILMS; ADHESION; STRESS; MODEL AB Cubic boron nitride (c-BN) is a material with significant technological potential based on material hardness, low chemical reactivity, high thermal conductivity and also on its very large band gap. However, these applications are limited by the poor adherence of the films. This study aims at trying to improve the vital problem of c-BN adherence. Cubic boron nitride (c-BN) films were deposited on Si (100) substrate by ion beam assisted deposition (IBAD). The substrate temperature was close to 250 degreesC. We have considered the strategy of changing the deposition parameters at the beginning of the c-BN nucleation in order to control the h-BN/c-BN interface and the stress in h-BN. The films were characterized by transmission electron microscopy (TEM) and infrared spectroscopy (FTIR). The adhesion of the films was measured by using the scratch test method. The optimization of deposition conditions at the early stages of the c-BN growth improves the adhesion of the films and enables growth of thicker films without peeling. We relate this improvement to structural modifications occurring in the films. TEM results could be explained on the basis of a new cubic-like phase located near the h-BN/a-BN interface triggered by an ion energy increase at the beginning of the c-BN nucleation. Eventually, we show that this energy increase leads to an increase of the c-BN content of the films. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Montpellier 2, CNRS, UMR 5650, Grp Etud Semicond, F-34095 Montpellier 5, France. ENSAM, Lab Bourguignon Mat & Proc, F-71250 Cluny, France. ENSAM, Lab Phys Chim Surfaces, F-49035 Angers, France. ISMRA, CNRS, URA 1317, Lab Etud & Rech Mat, F-14050 Caen, France. RP Masri, P, Univ Montpellier 2, CNRS, UMR 5650, Grp Etud Semicond, 2 CC 074,12 Pl E Bataillon, F-34095 Montpellier 5, France. TC 3 PD MAR-JUL PY 2001 VL 10 IS 3-7 SI Sp. Iss. SI BP 1357 EP 1362 UT ISI:000168730600207 ER PT J AU Younes, M Potiron, A TI A genetic algorithm for the shape optimization of parts subjected to thermal loading SO NUMERICAL HEAT TRANSFER PART A-APPLICATIONS ID SENSITIVITY ANALYSIS AB In many technical situations, the optimization of the mechanical behavior of structures proceeds from the search for the ideal shape satisfying thermal, mechanical, technological and geometrical constraints. In this article, the shape optimization of mono- and two-dimensional structures is handled by means of a new genetic algorithm (GA). The method is in general well suited to the resolution of nonconstrained optimization problems: The algorithm presented here has been modified by taking into account the imposed design constraints in the selection of the "individuals" belonging to a gives population. The crossover operation between individuals and the mutation process in their original forms are applied to derive the optimal shape of parts subjected to thermal loadings. The algorithm exhibits a good convergence toward the optimal solation and the numerical results of its application show a good numerical accuracy. C1 Ecole Natl Super Arts & Metiers, ERTGI, F-49035 Angers, France. RP Younes, M, Ecole Natl Super Arts & Metiers, ERTGI, 2 Blvd Ronceray,BP 3525, F-49035 Angers, France. TC 3 PD APR PY 2001 VL 39 IS 5 BP 449 EP 470 UT ISI:000168360000002 ER PT J AU Prunier, F Furber, AP Laporte, J Geslin, P TI Discovery of a parachute mitral valve complex (Shone's anomaly) in an adult SO ECHOCARDIOGRAPHY-A JOURNAL OF CARDIOVASCULAR ULTRASOUND AND ALLIED TECHNIQUES DE mitral valve anomaly; congenital heart disease; aortic coarctation; echocardiography AB Parachute mitral valve complex is an unusual congenital anomaly described by Shone et al. It is characterized by a parachute deformity of the mitral valve associated with additional forms of left heart anomalies, such as aortic valvular stenosis and coarctation. of the aorta. Fewer than 50 cases of Shone's complex have been reported in the literature, and it has only been observed in children. We report the case of a 33-year-old man, who was referred to our department because of atrial fibrillation. Echocardiographic evaluation and aortogram evidenced a! Shone's complex including a: parachute mitral valve anomaly, an aortic bicuspid valvular anomaly, and a coarctation of the aorta. C1 CHU Angers, Dept Cardiol, F-49033 Angers 01, France. RP Furber, AP, CHU Angers, Dept Cardiol, 4 Rue Larrey, F-49033 Angers 01, France. TC 3 PD FEB PY 2001 VL 18 IS 2 BP 179 EP 182 UT ISI:000168044100015 ER PT J AU Libouban, H Moreau, MF Legrand, E Basle, MF Audran, M Chappard, D TI Comparison insight dual X-ray absorptiometry (DXA), histomorphometry, ash weight, and morphometric indices for bone evaluation in an animal model (the orchidectomized rat) of male osteoporosis SO CALCIFIED TISSUE INTERNATIONAL DE DXA; bone histomorphometry; ash weight; male osteoporosis; orchidectomy ID OVARIECTOMIZED RATS; MINERAL DENSITY; MATURE RATS; MASS; ORCHIECTOMY; VERTEBRAE; RESORPTION; PRECISION; ACCURACY AB We have compared the measurements obtained by different methods: dual energy X-ray absorptiometry (DXA), histomorphometry, ash weight, and two morphometric indices (robusticity and bone weight/bone length index) in the orchidectomized (ORX) rat model of male osteoporosis. We examined 144 male wistar rats.: 48 shamoperated, 48 ORX, and 48 ORX-treated with a bisphosphonate (risedronate) 2 or 10 mug/kg/day, 5 days per week. Rats were sacrificed at 2, 4, 8, or 16 weeks after the beginning of the study. DXA was performed on a Hologic QDR 2000 on the whole body, whole tibia, and tibial metaphysis. Bone volumes (C.BV/C.TV, and BV/TV) were measured by histomorphometry on the proximal tibial. A significant correlation was obtained between weight measured by DXA and scale (r = 0.993, P < 0.000001), However, DXA underestimated weight by 0.3%. This discrepancy was dependent on the rat's weight. The weight bone length (WL) index was linearly correlated with BMD (r = 0.86), BMC (r = 0.96), and ash weight (r = 0.97). Correlation with robusticity was lower than with the WL index. A significant correlation was found between BMC of the metaphyseal region and the bone volumes but this explained only 27% of the variance; correlation with BMD was poorer (r = 0.40). BMC and ash weight were highly correlated (r = 0.992, P < 0.000001). However, DXA overestimated BMC by 11% and the overestimation was found to be clearly dependent on the net mineral content of the bone. C1 CHU Angers, Lab Histol Embryol, LHEA, F-49045 Angers, France. Fac Med, F-49045 Angers, France. CHU Angers, Serv Rhumatol, F-49033 Angers, France. RP Chappard, D, CHU Angers, Lab Histol Embryol, LHEA, F-49045 Angers, France. TC 3 PD JAN PY 2001 VL 68 IS 1 BP 31 EP 37 UT ISI:000167648300005 ER PT J AU Barbe, C Rochetaing, A Kreher, P TI Ischemic tolerance of the heart by adaptation to chronic hypoxia is suppressed by high subchronic carbon monoxide exposure SO INHALATION TOXICOLOGY ID RABBIT PAPILLARY-MUSCLE; HIGH-ALTITUDE; PULMONARY-HYPERTENSION; CARDIAC-HYPERTROPHY; GLOBAL-ISCHEMIA; URBAN ANGINA; RATS; ARRHYTHMIAS; MOUNTAINS; DISEASE AB This study was designed to investigate whether exposure to carbon monoxide (CO) could alter the ischemic tolerance induced by chronic hypoxia. We aimed to determine whether chronic hypoxia-induced cardiovascular adaptation was modified during the return to normoxia or by subchronic CO exposure. The degree of resistance to an in vitro transient ischemia was measured, using the Langendorff method, in hearts from rats previously exposed to chronic hypoxic hypoxia and/or subchronic CO exposure to 600 ppm. Chronic hypoxia decreased ischemic contracture (15.6 +/- 04.9 vs. 60.8 +/- 07.7%) and improved both contractile recovery (59.6 +/- 07.3 vs. 21.8 +/- 06.8%) and ventricular arrhythmia during reperfusion (0 vs. 45%) compared to a control normoxic group. However, in our chronic hypoxia regression model many parameters returned near to control values except for the persistence of cardiomegaly, a significant decrease in both ischemic contracture (22.0 +/- 04.9 vs. 60.8 +/- 07.7%), and ventricular tachycardia (25 vs. 45%). CO exposure alone increased the coronary flow and improved both contractile recovery (42.6 +/- 7.2 vs. 21.8 +/- 6.8%) and ventricular arrhythmia (16.7 vs. 45%) without altering the action potential shape. These two models causing tissue hypoxia induced the same degree of polycythemia or cardiomegaly and provided similar ischemic tolerance. CO exposure after chronic hypoxia exacerbated ischemic contracture (69.3 +/- 10.5 vs. 22.0 +/- 14.5%) and ventricular tachycardia incidence (100 vs. 50%) but with significant alteration in contractile recovery (12.7 +/- 10.5%) compared to the chronic hypoxia or CO exposure. Thus, CO exposure completely suppressed the chronic hypoxia-induced ischemic tolerance. C1 Fac Sci, Lab Preconditionnement & Remodelage Myocarde, F-49045 Angers, France. RP Rochetaing, A, Fac Sci, Lab Preconditionnement & Remodelage Myocarde, 2 Blvd Lavoisier, F-49045 Angers, France. TC 3 PD MAR PY 2001 VL 13 IS 3 BP 219 EP 232 UT ISI:000167169800003 ER PT J AU Kaassis, M Duquenne, M Croue, A Ronceray, J Rohmer, V Bigorgne, JC TI Calcitonin-secreting neuroendocrine carcinoma of the pancreas with invasion of the spleen and paraneoplasic hypercalcemia SO PRESSE MEDICALE C1 CHU Angers, Serv Endocrinol & Med Interne, F-49033 Angers 01, France. CHU Angers, Anat Pathol Lab, F-49033 Angers, France. CHU Angers, Serv Chirurg Viscerale, F-49033 Angers 01, France. RP Kaassis, M, CHU Angers, Serv Med A, 4 Rue Larrey, F-49033 Angers 01, France. TC 3 PD JAN 13 PY 2001 VL 30 IS 1 BP 24 EP 24 UT ISI:000166660300007 ER PT J AU Pourcher, AM Sutra, L Hebe, I Moguedet, G Bollet, C Simoneau, P Gardan, L TI Enumeration and characterization of cellulolytic bacteria from refuse of a landfill SO FEMS MICROBIOLOGY ECOLOGY DE cellulolytic bacteria; landfill site; phenotypic analysis; 16S rDNA sequence analysis ID METHANE PRODUCTION; MICROBIAL ACTIVITY; MUNICIPAL REFUSE; SOLID-WASTE; SP-NOV; PROPOSAL; CELLULOMONAS; AUREOBACTERIUM; CLOSTRIDIA; DIVERSITY AB Enumeration and phenotypic characterization of aerobic cellulolytic bacteria were performed on fresh. 1 year old and 5 years old refuse samples of a French landfill site. Numbers of cellulolytic bacteria ranged From 1.1 x 10(6) to 2.3 x 10(8) c.f.u. (g dry wt.)(-1) and were lower in 5 years old refuse samples. A numerical analysis of phenotypic data based on 80 biochemical tests and per-formed on 321 Gram-positive isolates fi om refuse, revealed a high phenotypic diversity of cellulolytic bacteria which were distributed into 21 clusters. Based on the phenotypic analysis and the sequencing of 16S rDNA of five representative strains of major clusters, the predominant cellulolytic groups could be assigned to the family of Bacillaceae and to the genera Cellulomonas,Microbacterium and Lactobacillus. Furthermore, chemical parameters such as pH, carbohydrates and volatile solid contents influenced the composition of the cellulolytic bacterial groups which were reduced essentially to the family of Bacillaceae in the oldest refuse samples. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. C1 Univ Angers, Lab Sci Environm & Amenagement, Fac Sci, UFR Sci, F-49045 Angers 01, France. Univ Beaucouze, UMR Pathol Vegetale, INRA, INH, F-49071 Beaucouze, France. Fac Med Marseille, F-13385 Marseille 05, France. RP Pourcher, AM, Univ Angers, Lab Sci Environm & Amenagement, Fac Sci, UFR Sci, 2 Blvd Lavoisier, F-49045 Angers 01, France. TC 3 PD JAN PY 2001 VL 34 IS 3 BP 229 EP 241 UT ISI:000166468400007 ER PT J AU Bonamy, L Buldyreva, JV TI Non-Markovian far-wing rotational Raman spectrum from translational modeling SO PHYSICAL REVIEW A ID GASEOUS NITROGEN; THEORETICAL-ANALYSIS; INELASTIC RATES; Q-BRANCH; TEMPERATURE; SCATTERING; ABSORPTION; COLLISIONS; RELAXATION; CO2 AB An improved version of the recently developed non-Markovian energy corrected sudden (ECS) model [J. V. Buldyreva and L. Bonamy, Phys. Rev. A 60, 370 (1999)] is proposed to ensure good accuracy of the quasiexponential behavior of the scattered light intensity vs frequency that is observed experimentally in the far wings of nitrogen spectra. To this end, the more refined translational spectrum proposed by Birnbaum and Cohen [Can. J. Phys. 54, 593 (1976)] is used as the adiabaticity factor. At low frequencies it reduces to the traditional one-parameter Lorentzian shape which describes the long-time translational dynamics and allows the basic ECS transition rates to be identified. At high frequencies it introduces an additional parameter which describes the short-time dynamics. The value of this parameter at room temperature and at low temperature (150 K) is obtained by fitting the far-wing theoretical intensities of the depolarized nitrogen spectra to their experimental values. This value compares favorably with its theoretical evaluation. C1 Univ Franche Comte, Phys Mol Lab, CNRS, UMR 6624, F-25030 Besancon, France. Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, UMR 6136, F-49045 Angers, France. RP Bonamy, L, Univ Franche Comte, Phys Mol Lab, CNRS, UMR 6624, 16 Route Gray, F-25030 Besancon, France. EM lionel.bonamy@univ-fcomte.fr TC 3 PD JAN PY 2001 VL 6301 IS 1 AR 012715 DI ARTN 012715 UT ISI:000166383000068 ER PT J AU Brunel, M Ameur, KA Sanchez, F TI Optimization of the transverse concentration profile in optical limiting devices SO OPTICS COMMUNICATIONS DE optical limiting; reverse saturable absorption ID REVERSE SATURABLE ABSORPTION AB A new design of optical limiters is presented. The transverse concentration profiles that allow to use the potentiality of reverse saturable absorbers to the best advantage are determined. The problem is formulated in a variational form and then solved analytically using the calculus of variations. Relatively high nonlinearities together with thick samples can be considered using a decomposition in Adomian polynomials. This method can be extended to other nonlinear optical problems where the transverse concentration profile of molecules has to be adjusted to best advantage. (C) 2001 Published by Elsevier Science B.V. C1 Univ Rouen, CORIA UMR 6614, Grp Opt & Optron, F-76821 Mt St Aignan, France. ISMRA, CIRIL, F-14050 Caen 4, France. Univ Angers, Lab POMA, F-49045 Angers 01, France. RP Brunel, M, Univ Rouen, CORIA UMR 6614, Grp Opt & Optron, Pl Emile Blondel, F-76821 Mt St Aignan, France. TC 3 PD JAN 1 PY 2001 VL 187 IS 1-3 BP 271 EP 276 UT ISI:000166371700030 ER PT J AU Berteloot, F Loeb, JJ TI A geometrical characterization of Lattes examples in P-k SO BULLETIN DE LA SOCIETE MATHEMATIQUE DE FRANCE ID COMPLEX DYNAMICS; RATIONAL MAPS; POINTS AB A Lattes example is an holomorphic self-map of the complex projective space which may be lift to some dilation of the affine space with same dimension by a ramified cover on which fibers a cristallographic group is acting transitively. We show that every holomorphic self-map of the complex projective space whose Green current is smooth and strictly positive on some non empty open set is a Lattes example. C1 Univ Toulouse 3, Lab E Picard, F-31062 Toulouse, France. Univ Angers, Fac Sci, Dept Math, F-49045 Angers, France. TC 2 PY 2001 VL 129 IS 2 BP 175 EP 188 UT ISI:000182062500003 ER PT J AU Vasquez, M Hao, JK TI A hybrid approach for the zero-one multidimensional knapsack problem SO RAIRO-RECHERCHE OPERATIONNELLE-OPERATIONS RESEARCH ID PROGRAMMING PROBLEMS; ALGORITHM AB We present, in this article, a hybrid approach for solving the, 0-1 multidimensional knapsack problem (MKP). This approach combines linear programming and Tabu search. The resulting algorithm improves on the best result on many well-known hard benchmarks. C1 LGI2P, F-30035 Nimes 1, France. Univ Angers, LERIA, F-49045 Angers, France. RP Vasquez, M, LGI2P, Parc Sci Georges Besse, F-30035 Nimes 1, France. TC 2 PD OCT-DEC PY 2001 VL 35 IS 4 BP 415 EP 438 UT ISI:000175135900004 ER PT J AU Rouge-Maillart, C Tracqui, A Tortel, MC Pessaux, P Penneau, M Ludes, B TI Fatal blunt pancreatic trauma secondary to assault and battery: a case report SO INTERNATIONAL JOURNAL OF LEGAL MEDICINE DE blunt abdominal trauma; pancreatic injury; violence; diagnosis ID ABDOMINAL-TRAUMA; CHILD-ABUSE; INJURIES; DIAGNOSIS AB The authors report on an fatal case of closed trauma of the pancreas in a context of violence. A 55-yearold man was found unconscious on the sidewalk and died a short time after being taken to the hospital. He had been hit with several punches to the face and abdomen 6 h before. The post-mortem examination showed numerous bruises over the whole body, a haemoperitoneum, a fissuration of the spleen and a massive peripancreatic haemorrhage associated with a complete dilaceration of the pancreas head. Histological examination of the pancreas revealed a massive necrosis associated with a subtotal disappearance of the acini, numerous sites of cytosteatonecrosis and a large haemorrhagic suffusion of the peripancreatic tissue. This case illustrates the possibilities of pancreatic injuries induced by blunt force aimed at the abdomen in a context of violence. Even if this occurs as an isolated injury it can result in rapid death because of the particular type of pancreatic fracture which is frequently involved. In postmortem situations, the pancreas should be systematically checked at necropsy and a histological examination should be carried out at the slightest doubt of a pancreatic lesion or suspicion of blunt force abdominal injuries. C1 Univ Hosp Angers, Emergency Dept, Unity Forens Med, F-49100 Angers, France. Fac Med Strasbourg, Inst Forens Med, Strasbourg, France. RP Rouge-Maillart, C, Univ Hosp Angers, Emergency Dept, Unity Forens Med, F-49100 Angers, France. TC 2 PD DEC PY 2001 VL 115 IS 3 BP 162 EP 164 UT ISI:000175091900007 ER PT J AU Devys, A Lortholary, A Audran, M TI PTHrP and breast cancer SO BULLETIN DU CANCER DE PTHrP; hypercalcemia; bone metastases; breast cancer ID HORMONE-RELATED PROTEIN; PARATHYROID-HORMONE; HUMORAL HYPERCALCEMIA; BONE METASTASES; MALIGNANCY; RECEPTOR; TUMORS; BISPHOSPHONATE; IDENTIFICATION; LOCALIZATION AB Parathyroid hormone-related peptide (PTHrP) has a high homology with the N-terminal portion of the parathyroid hormone (PTH). The gene of PTHrP is complex and can generate by alternative splicing at least three mature peptides containing 139, 141 and 173 amino acids. PTHrP acts via a common receptor with PTH but also via specific receptors. In physiological circumstances, PTHrP is produced locally in many paracrine functions, particularly during embryonic normal tissues where it has autocrine/ development, growth regulation and differentiation of many cellular types. PTHrP has endocrine action on bone and kidney. The humoral hypercalcemia of malignancy is mainly mediated by PTHrP, Most hypercalcemic patients with solid tumors have increased plasma PTHrP, whereas PTHrP is not detectable in healthy subjects. During treatment with bisphosphonates, elevated plasma levels of PTHrP are associated with a weak response. PTHrP has also a significant role in the pathophysiology of bone metastases. PTHrP can induce a local osteolysis near the bone metastases, which favours their progression and thus participates in the autocrine regulation of tumor growth. In breast cancer, PTHrP is detected in about 60% of primary tumors and in more than 70% of bone metastases, whereas only Coo of nonbone metastases express PTHrP. A higher expression of PTHrP and its mRNA 1-139, is positively correlated with an invasive tumor phenotype and the development of bone metastases. PTHrP is an effector of transforming growth factor (TGF beta) in the development and progression of osteolytic hone metastases. TGF beta, which is released in bone matrix during osteolytic resorption, enhances tumor cells PTHrP production. Then, PTHrP stimulates bone resorption and develops tumor cells metastatic potential. Thus a feedback loop exists between carcinoma cell and the bone microenvironment, leading to a vicious circle. C1 Ctr Paul Papin, F-49033 Angers 01, France. CHU Angers, Lab Rhumatol, F-49033 Angers, France. RP Devys, A, Ctr Paul Papin, 2 Rue Moll, F-49033 Angers 01, France. TC 2 PD NOV PY 2001 VL 88 IS 11 BP 1075 EP 1080 UT ISI:000172837800006 ER PT J AU Cales, P TI Hepatitis B vaccination and serious side-effects: sequence is not consequence SO GASTROENTEROLOGIE CLINIQUE ET BIOLOGIQUE ID NERVOUS-SYSTEM DEMYELINATION C1 CHU Angers, Serv Hepatogastroenterol, Angers, France. RP Cales, P, CHU Angers, Serv Hepatogastroenterol, Angers, France. TC 2 PD OCT PY 2001 VL 25 IS 10 BP 859 EP 862 UT ISI:000172956800002 ER PT J AU Benec'h, S Buldyreva, J Chrysos, A TI Pressure broadening and temperature dependence of microwave and far infrared rotational lines in OH perturbed by N-2, O-2, and Ar SO JOURNAL OF MOLECULAR SPECTROSCOPY DE pressure broadening; infrared rotational lines; microwave rotational lines; free hydroxyl radical OH; exact trajectory approach ID H-2; HE AB Absorption linewidths associated with microwave transitions across A-doublets and pure rotational transitions of the free hydroxyl radical OH perturbed by N-2, O-2, and Ar are computed for temperatures ranging from 194 to 300 K within the framework of the Robert-Bonamy semiclassical formalism using exact trajectories. The calculation is done for the Hund's coupling case (a) which is expected to be a good approximation for the low rotational quantum numbers so far experimentally studied. As intermolecular potential, the usual sum of electrostatic and atom-atom interactions is taken. Unlike previous theoretical predictions, our computed values are found to be in good agreement with measurements. (C) 2001 Elsevier Science. C1 Univ Angers, UMR CNRS 6136, Lab Proprietes Opt Mat & Applicat, F-49045 Angers, France. RP Benec'h, S, Univ Angers, UMR CNRS 6136, Lab Proprietes Opt Mat & Applicat, 2 Blvd Lavoisier, F-49045 Angers, France. TC 2 PD NOV PY 2001 VL 210 IS 1 BP 8 EP 17 UT ISI:000172726700002 ER PT J AU Azouvi, P Didic-Hamel, CM Fluchaire, I Godefroy, O Hoclet, E Le Gall, D Marie, RM Meulemans, T Naegele, B Peres, B Pillon, B CA GREFEX TI The assessment of executive functions in clinical practice SO REVUE DE NEUROPSYCHOLOGIE DE frontal lobe; executive functions; tests ID FRONTAL-LOBE LESIONS; DUAL-TASK PERFORMANCE; CARD SORTING TEST; RANDOM NUMBER GENERATION; TRAUMATIC BRAIN INJURY; CLOSED-HEAD-INJURY; PROGRESSIVE SUPRANUCLEAR PALSY; DELAYED-RESPONSE TASKS; VERBAL WORKING-MEMORY; SERIAL-ADDITION TASK AB The approach of executive functions began with the early description of behavioural disorders induced by damage to the frontal lobes. The development of experimental neuropsychology has led to the description of a large variety of cognitive disorders in patients with frontal lobe damage. Such approach has generated a large number of tests which form the basis of the present clinical assessment of executive functions. Despite the clear interest of tests derived from experimental studies, their relevance for clinical practice remains poorly examined. The aim of this study was to review the tests usually used in clinical practice and to examine the validation studies, We used data from our collaborative group, from the literature, and from a short questionnaire sent to several French-speaking University groups. Tests used to assess inhibition, set-shifting, rules deduction, concept formation, planning, information generation, nonspatial attention, central executive of working memory, strategic processes in episodic memory, cognitive estimation, ecological tests and batteries assessing executive function are reviewed. So far, only a few tests have benefited from more than one validation studies; diagnosis criteria, sensitivity, specificity, and reliability are rarely available. Most validation studies are based on the comparison of anterior and posterior brain-damaged patients. This review emphasises the need to reexamine the clinical accuracy of most tests assessing executive functions according to another gold standard such as patient incapacity, and to determine standardised criteria of the dysexecutive syndrome. C1 Hop R Poincare, Serv Reeduc Neurol, Garches, France. Serv Neurol & Neuropsychol, Marseille, France. INSERM, E 9926, F-13258 Marseille, France. Serv Neurol & Neuropsychol, Grenoble, France. Serv Neurol, EA 2691, Amiens, France. CMUDD, St Hilaire Du Touvet, France. Dept Neurol, Angers, France. Dept Psychol, EA 2646, Angers, France. Serv Neurol Dejerine, Caen, France. INSERM, U320, Caen, France. Fac Psychol, Serv Neuropsychol, Liege, Belgium. Serv Neurol, Grenoble, France. Hop La Pitie Salpetriere, INSERM, U007, Paris, France. TC 2 PD SEP PY 2001 VL 11 IS 3 BP 383 EP 433 UT ISI:000172464200001 ER PT J AU Roquelaure, Y Gabignon, Y Gillant, JC Delalieux, P Ferrari, C Mea, M Fanello, S Penneau-Fontbonne, D TI Transient hand paresthesias in Champagne vineyard workers SO AMERICAN JOURNAL OF INDUSTRIAL MEDICINE DE musculoskeletal disorders; work-related; agriculture; winegrowing; pruning shears; epidemiology ID CARPAL-TUNNEL-SYNDROME; MUSCULOSKELETAL DISORDERS; PREVALENCE; IMPACT; MODEL AB Background The aim of the study was to assess the prevalence of hand paresthesias (HP) and their relationship with pruning activities. Methods A cross-sectional study was conducted among 537 workers pruning grapevines in the region of Champagne. All workers completed a questionnaire about nocturnal HP and musculoskeletal pain during the preceding 12-month period. Results The 12-month prevalence of nocturnal HP and hand-wrist pain were 37 and 12%, respectively. HP, predominantly affecting the dominant hand, only began during the pruning period and ended after the pruning season in 90% of cases. HP were transient in most cases, with a mean duration of symptoms of 3.3 +/- 3.2 months. Risk factors associated with HP were: female gender (OR = 2.3 [1.3-3.0]), being overweight (OR = 1.6 [1.1-2.5]), payment on a piecework basis (OR = 2.0 [1.2-2.3]) and traditional blade sharpening method (OR = 1.7 [1.1-2.7]). HP were less frequent in employees who used electric pruning shears (OR = 0.5 [0.2-1.6], P = 0.09). Conclusions The development of HP, which affected a third of employees, was different from HP observed in industrial workers since most vineyard workers recovered without medical treatment after the pruning season. (C) 2001 Wiley-Liss, Inc. C1 CHU Angers, Consultat Pathol Profess & Ergon, Univ Hosp, F-49033 Angers, France. RP Roquelaure, Y, CHU Angers, Consultat Pathol Profess & Ergon, F-49033 Angers, France. TC 2 PD DEC PY 2001 VL 40 IS 6 BP 639 EP 645 UT ISI:000172480300003 ER PT J AU Beydon, L TI Do away with alarms or make them intelligent? SO ANNALES FRANCAISES D ANESTHESIE ET DE REANIMATION C1 CHU Angers, Dept Anesthesie, F-49033 Angers 01, France. RP Beydon, L, CHU Angers, Dept Anesthesie, F-49033 Angers 01, France. TC 2 PD OCT PY 2001 VL 20 IS 8 BP 667 EP 668 UT ISI:000171963300001 ER PT J AU Kityk, IV Sahraoui, B Ledoux-Rak, I Salle, M Migalska-Zalaz, A Kazu, T Gorgues, A TI Push-pull chromophores incorporated in 1,3-dithiol-2-ylidene moiety as new electrooptics materials SO MATERIALS SCIENCE AND ENGINEERING B-SOLID STATE MATERIALS FOR ADVANCED TECHNOLOGY DE push-pull chromophores; 1,3-dithiol-2-ylidene; electrooptical coefficients ID SINGLE-CRYSTALS AB Experimental and theoretical investigations of influence of the push-pull chromophores incorporated in the 1.3-dithiol-2-ylidene moiety as the electron donating part on linear electrooptic (Pockels) coefficient have been performed. The proposed theoretical approach is based on geometry optimization of the molecule and appropriate quantum chemical calculations. Intermolecular interactions are evaluated within a framework of long-range intermolecular interactions. Based on approach combining local density approximation (LDA) and Hartree-Fock method we have revealed good correlation between the experimental and theoretical values of linear electrooptical coefficients (EOC). Contribution of electronic UV and phonon modes to EOC tensor component r(111) (lambda = 633 mn) was evaluated. The phonon and UV modes have been found to be very sensible to substitution of the chromophores. Analysis of contributions of particular molecular group is done. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, CNRS EP 130, Lab Proprietes Opt Mat & Applicat, F-49045 Angers, France. Univ Angers, UMR CNRS 6501, Lab Ingn Mol & Mat Organ, F-49045 Angers, France. France Telecom, Ctr Natl Etud Telecommun, F-92220 Bagneux, France. Tohoku Univ, Hyndao Comp Sci Ctr, Tokyo, Japan. RP Kityk, IV, Univ Angers, CNRS EP 130, Lab Proprietes Opt Mat & Applicat, 2 Bd Lavoisier, F-49045 Angers, France. TC 2 PD NOV 15 PY 2001 VL 87 IS 2 BP 148 EP 159 UT ISI:000171821600007 ER PT J AU Genet, S Costalat, R Burger, J TI The influence of plasma membrane electrostatic properties on the stability of cell ionic composition SO BIOPHYSICAL JOURNAL ID ELECTROGENIC SODIUM-PUMP; SURFACE-CHARGES; CARDIAC MYOCYTES; DIVALENT IONS; GUINEA-PIGS; CALCIUM; CHANNEL; INHIBITION; NEURONS; PH AB An electro-osmotic model is developed to examine the influence of plasma membrane superficial charges on the regulation of cell ionic composition. Assuming membrane osmotic equilibrium, the ion distribution predicted by Gouy-Chapman-Grahame (GCG) theory is introduced into ion transport equations, which include a kinetic model of the Na/K-ATPase based on the stimulation of this ion pump by internal Na+ ions. The algebro-differential equation system describing dynamics of the cell model has a unique resting state, stable with respect to finite-sized perturbations of various types. Negative charges on the membrane are found to greatly enhance relaxation toward steady state following these perturbations. We show that this heightened stability stems from electrostatic interactions at the inner membrane side that shift resting state coordinates along the sigmoidal activation curve of the sodium pump, thereby increasing the pump sensitivity to internal Na+ fluctuations. The accuracy of electrostatic potential description with GCG theory is proved using an alternate formalism, based on irreversible thermodynamics, which shows that pressure contribution to ion potential energy is negligible in electrostatic double layers formed at the surfaces of biological membranes. We discuss implications of the results regarding a reliable operation of ionic process coupled to the transmembrane electrochemical gradient of Na+ ions. C1 Univ Paris 06, INSERM, U483, F-75252 Paris 05, France. Univ Angers, Lab Ingn Syst Automatises, F-49000 Angers, France. RP Genet, S, Univ Paris 06, INSERM, U483, Boite 23,9 Quai St Bernard, F-75005 Paris, France. TC 2 PD NOV PY 2001 VL 81 IS 5 BP 2442 EP 2457 UT ISI:000171755200002 ER PT J AU Coindre, JM Blanc-Vincent, MP Collin, F MacGrogan, G Balaton, A Voigt, JJ TI Standards, Options and Recommendations: practice guidelines for difficult diagnosis in surgical pathology or cytopathology in cancer patients SO BULLETIN DU CANCER DE surgical pathology; cytopathology; diagnosis; guidelines; neoplasms; cancer ID INTER-OBSERVER VARIATION; SOFT-TISSUE SARCOMAS; INTEROBSERVER VARIABILITY; HISTOPATHOLOGIC DIAGNOSIS; AMENDED REPORTS; BREAST-LESIONS; REPRODUCIBILITY; CLASSIFICATION; ONCOLOGY; QUALITY AB Context: The "Standards, Options and Recommendations" (SOR) project, started in 1993 is a collaboration between the Federation of the French Cancer Centres (FNCLCC), the 20 French Cancer Centres and specialists from French Public Universities, General Hospitals and Private Clinics. The main objective is the development of clinical practice guidelines to improve the quality of health care and outcome for cancer patients. The methodology is based on literature review and critical appraisal by a multidisciplinary group of experts, with feedback from specialists in cancer care delivery. Objectives: To develop clinical practice guidelines according to the definitions of the Standards, Options and Recommendations project for difficult diagnoses in surgical pathology or cytopathology in cancer patients. Methods: Data were identified by searching Medline (R) and using the personal reference lists of members of the expert groups. Once the guidelines were defined, the document was submitted for review to 71 independent reviewers. Results: The main recommendations to prevent and reduce the number of difficult diagnoses in surgical pathology or cytopathology are. 1) The development of quality insurance programs with use of written procedures in each pathology laboratory (standard). 2) The knowledge of clinical data in order to explain surgical pathology or cytopathology results (standard). 3) The availability of complementary patient informations (radiologic data...) can be useful to explain surgical pathology or cytopathology results (option). The main recommendations to detect lesions associated with difficult diagnosis in surgical pathology or cytopathology are: 1) Tumor types known as potential difficult diagnosis in surgical pathology or cytopathology shoud be reviewed by a second pathologist. 2) The systematic second reviewing for every case is expensive but has to be done when the difficulty is know (sarcoma, lymphoma...) by experienced pathologists. The main recommendations to solve difficult diagnosis in surgical pathology or cytopathology are: 1) Block recuts, use of special techniques (immunocytohistochemistry and molecular biology), additional data from clinicians, second opinion by a local pathologist, or new specimen can be required for establishing the diagnosis (options). 2) Outside second opinion by expert pathologist has to be considered once the other steps did not allow to establish surgical or cytopathology diagnosis (recommendations, expert agreement). C1 Inst Bergonie, Bordeaux, France. FNCLCC, Paris, France. Ctr Georges Francois Leclerc, Dijon, France. Ctr Pathol, Bievres, France. Inst Claudis Regaud, Toulouse, France. Ctr Reg Leon Berard, Lyon, France. Ctr Rene Huguenin, St Cloud, France. Ctr Val Aurelle, Montpellier, France. Ctr Paul Strauss, Strasbourg, France. Inst Gustave Roussy, Villejuif, France. CHU, St Etienne Du Rouvray, France. Inst Curie, Paris, France. Ctr Paul Papin, Angers, France. RP Coindre, JM, Inst Bergonie, Bordeaux, France. TC 2 PD AUG PY 2001 VL 88 IS 8 BP 765 EP 773 UT ISI:000171138300008 ER PT J AU Do Thanh, X Djebbar-Sid, S Duchene, S Benali-Baitich, O Bouet, G Khan, MA TI Chronotoxicity of a copper (II) complex with a linear tetradentate ligand in mice SO BIOLOGICAL RHYTHM RESEARCH DE Swiss mice; copper (II); complex; chronotoxicity ID RENAL ALKALINE-PHOSPHATASE; SPRING TIME; RATS; CHRONOBIOLOGY AB In vitro copper (II) complex presents antimitotic, effects. In this work, we have studied the in vivo seasonal toxic effects of copper (II), ligand (H2L) and the complex [Cu(H2L)(H2O)(2)]Cl-2. 4H(2)O in male Swiss mice. During spring, an i.p. injection of CuCl2 in aqueous NaCl (9 g(.)l(-1)) up to 0.05 mu mol(.)kg(-1) b.w. (body weight) killed 60% of the rodents after 6 days. LD100 was up to 0.3 mu mol(.)kg(-1); H2L was well tolerated, while the complex was 30% lethal with 50 mu mol(.)kg(-1). In autumn, mice were less sensitive to CUCl2, and both ligand and complex were equally tolerated and this leads to the conclusion that, in vivo, chronotoxicities of copper (II) and complex in NaCl aqueous solutions are quite different in spring and autumn seasons. C1 Fac Pharm Angers, Coordinat Lab, F-49100 Angers, France. CHU Angers, Pharmacol Lab, Angers, France. USTHB, Coordinat Lab, Algiers, Algeria. RP Do Thanh, X, Fac Pharm Angers, Coordinat Lab, 16 Blvd Daviers, F-49100 Angers, France. TC 2 PD OCT PY 2001 VL 32 IS 4 BP 423 EP 429 UT ISI:000170705800003 ER PT J AU Fondrinier, E Boisdron-Celle, M Chassevent, A Lorimier, G Gamelin, E TI Experimental assessment of tumor growth and dissemination of a microscopic peritoneal carcinomatosis after CO2 peritoneal insufflation or laparotomy - Clinical and biological results of a randomized animal study SO SURGICAL ENDOSCOPY-ULTRASOUND AND INTERVENTIONAL TECHNIQUES DE cancer; laparoscopy; laparotomy; peritoneal carcinomatosis ID PORT-SITE METASTASES; LAPAROSCOPIC SURGERY; RECTAL-CANCER; MURINE MODEL; RAT MODEL; PNEUMOPERITONEUM; IMPLANTATION; RECURRENCE; CELLS; GALLBLADDER AB Background: Based on clinical observations and previous animal studies, laparoscopic surgery for malignant disease is regarded as controversial. We used a rat model to measure and compare the tumor growth, proliferation, and dissemination of a microscopic peritoneal carcinomatosis after CO2 intraperitoneal insufflation or laparotomy. Methods: Peritoneal carcinomatosis was induced in three groups of 27 BD IX rats each with intraperitoneal injections of 10(6) DHD/K12 cells, an aneuploid tumor cell line. At 48 h after tumor cell injection, the animals were randomly divided into three groups to undergo different types of intervention. All animals were anesthetized for 20 min (Halothane). The control group had no surgical intervention (group C), group I had CO2 insufflation (7 mmHg),and group L had a midline laparotomy (5-cm). Neither bowel manipulation nor any other traumatic action was performed. Two weeks later, the rats were killed and the incidence, type, and dissemination of carcinomatosis were evaluated. We also measured the tumor's weight. Malignant omentum was sampled for flow cytometry analysis (DNA ploidy, S-phase fraction). Results: The incidence of carcinomatosis did not differ among the groups. The mean score of macroscopic characteristics of the carcinomatosis was 2.8 +/- 1.9 in group L. 2.9 +/- 1.9 in group I, and 3 +/- 1.9 in group C (NS). The location of the implants did not differ, except for parietal peritoneum location, which was more frequent in group L (p < 0.01). The tumor weight was 4.96 +/- 3.2 in group L, 5.55 +/- 3.2 in group C, and 5.75 +/- 3.4 in group I (NS). The percentage of aneuploid cells and S-phase fraction did not differ statistically among the groups. Conclusion: These results indicate that CO2 insufflation does not cause more effects than laparotomy when tumors cells are present before the beginning of the surgery. Further studies are needed to determine the influence of other steps in laparoscopic surgery on tumor growth and dissemination. C1 Ctr Reg Lutte Contre Canc Paul Papin, Dept Surg, F-49033 Angers, France. RP Fondrinier, E, Ctr Reg Lutte Contre Canc Paul Papin, Dept Surg, 2 Rue Moll, F-49033 Angers, France. TC 2 PD AUG PY 2001 VL 15 IS 8 BP 843 EP 848 UT ISI:000170319300015 ER PT J AU Meo, M TI Residues in the not necessarily complete intersection case SO COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE I-MATHEMATIQUE AB We get explicitly a decomposition of Serre-Grothendieck residue type for the integration current associated to an irreducible analytic subset of an open subset of the affine space. To this end we begin with proving a generalized King formula: by blowing zip we are led to the Poincare-Lelong formula. The formula that we obtain which also follows from the Siu decomposition formula for closed positive currents, allows us to extend to the general case formulas for the current of integration already known in the case of a complete intersection, such as the Berenstein-Yger formula using the Gelfand-Atiyah-Kashiwara meromorphic continuation and the Coleff-Herrera-Passare formula with residual integrals. (C) 2001 Academic des sciences/Editions scientifiques et medicales Elsevier SAS. C1 Univ Angers, Dept Math, F-49045 Angers 01, France. RP Meo, M, Univ Angers, Dept Math, 2 Blvd Lavoisier, F-49045 Angers 01, France. TC 2 PD JUL 1 PY 2001 VL 333 IS 1 BP 33 EP 38 UT ISI:000170225300007 ER PT J AU Aqad, E Leriche, P Mabon, G Gorgues, A Khodorkovsky, V TI Novel D-pi-A chromophores based on the fulvene accepting moiety SO ORGANIC LETTERS ID NONLINEAR OPTICAL-PROPERTIES AB [GRAPHICS] Novel D-pi -A chromophores based on the fulvene accepting moiety and p-dimethylamino phenyl and 1,3-dithiole-2-ylidene donor moieties have been prepared. The X-ray structures of two representative derivatives have been determined. Examination of the UV-visible spectra and cyclic voltamperometry data revealed remarkable sensitivity of the electronic structure of these derivatives to substituents at the cyclopentadiene ring. C1 Univ Angers, UFR Sci, F-49045 Angers, France. Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel. RP Leriche, P, Univ Angers, UFR Sci, F-49045 Angers, France. TC 2 PD JUL 26 PY 2001 VL 3 IS 15 BP 2329 EP 2332 UT ISI:000170059000019 ER PT J AU Tuech, JJ Pessaux, P Arnaud, JP TI Portal vein resection in pancreatic head carcinoma - Part 2: Clinical significance SO HEPATO-GASTROENTEROLOGY DE pancreas; portal vein; extended pancreatectomy; pancreaticoduodenectomy; surgery; portal vein resection ID DUCTAL ADENOCARCINOMA; EXTENDED PANCREATECTOMY; SURGICAL-TREATMENT; LYMPHATIC FLOW; CANCER; PANCREATICODUODENECTOMY; SURVIVAL; LYMPHADENECTOMY; EXPERIENCE; INVASION AB The prognosis of pancreatic carcinoma remains poor. Surgical resection provides the only chance for cure. Prognostic factors and survival are similar between patients who underwent portal vein resection compared with standard resection. Isolated portal vein involvement should not be a contraindication for pancreatic resection. C1 Angers Univ Hosp, Dept Digest Surg, F-49000 Angers, France. RP Tuech, JJ, Angers Univ Hosp, Dept Digest Surg, 4 Rue Larrey, F-49000 Angers, France. TC 2 PD MAY-JUN PY 2001 VL 48 IS 39 BP 888 EP 891 UT ISI:000169631600067 ER PT J AU Lupien, B Lauzon, E Desrochers, C TI PLS modelling of strength and optical properties of newsprint at Papier Masson Ltee - The technique helped to identify sources of variation SO PULP & PAPER-CANADA DE newsprint; strength properties; optical properties; models; variables; optimization AB A study was conducted on the long-term variation in paper strength and optical properties for Papier Masson Ltee, a newsprint mill, to identify the important variables and define optimum targets. A PLS technique was used by mill engineers to identify sources of variation in paper quality. It provided clear understanding that helped during quality upsets. C1 Papier Masson Ltee, Masson Angers, PQ, Canada. RP Lupien, B, Papier Masson Ltee, Masson Angers, PQ, Canada. TC 2 PD MAY PY 2001 VL 102 IS 5 BP 19 EP 21 UT ISI:000169182900004 ER PT J AU Roubtsov, VN Roulstone, I TI Holomorphic structures in hydrodynamical models of nearly geostrophic flow SO PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES DE holomorphic function; Hamiltonian structure; hydrodynamics; Kahler geometry; special Lagrangian coordinates; Monge-Ampere equations ID MONGE-AMPERE EQUATIONS; CONTACT TRANSFORMATIONS; SEMIGEOSTROPHIC THEORY; INTERMEDIATE MODELS; GEOMETRY AB We study complex structures arising in Hamiltonian models of nearly geostrophic flows in hydrodynamics. In many of these models an elliptic Monge Ampere equation defines the relationship between a 'balanced' velocity field, defined by a constraint in the Hamiltonian formalism, and the materially conserved potential vorticity. Elliptic Monge Ampere operators define an almost-complex structure, and in this paper we show that a natural extension of the so-called geostrophic momentum transformation of semi-geostrophic theory, which has a special importance in theoretical meteorology, defines Kahler and special Kahler structures on phase space. Furthermore, analogues of the 'geostrophic momentum coordinates' are shown to be special Lagrangian coordinates under conditions which depend upon the physical approximations under consideration. Certain duality properties of the operators are studied within the framework of the Kahler geometry. C1 Univ Angers, Dept Math, F-49045 Angers, France. ITEP, Moscow 117259, Russia. Univ Reading, Meteorol Off, Joint Ctr Mesoscale Meteorol, Reading RG6 6BB, Berks, England. RP Roubtsov, VN, Univ Angers, Dept Math, 2 Blvd Lavoisier, F-49045 Angers, France. TC 2 PD JUN 8 PY 2001 VL 457 IS 2010 BP 1519 EP 1531 UT ISI:000169195000015 ER PT J AU Hayek, G Mercier, P Fournier, HD Menei, P Pouplard, F Guy, G TI Dermal sinus and dermoid cyst revealed by abscess formation in posterior fossa. Report of two pediatric cases and review of the litterature SO NEUROCHIRURGIE DE dermal sinus; dermoid cyst; cerebellar abscess; computed tomography; magnetic resonance imaging; posterior fossa AB Cranial dermal sinus, usually associated with dermoid cyst, is the persistance of an abnormal embryonal communication between the skin and the central nervous system,. It may be the source of intracranial infection, most often a meningitis and rarely an abscess formation. Two cases of little girls (18 months and 2 years) having dermal sinus with dermoid cyst revealed by cerebellar abscess formations are reported. In the first case there were multiple cerebellar abscesses with hydrocephalus leading to a raised intracranial pressure. In the second case there was an abscess formation adjacent to the dermoid cyst CT scan showed cysts and abscesses but MRI, achieved in the second case, was useful in demonstrating the sinus tract as well as tire associated cyst and abscess. The two patients underwent a posterior fossa surgery with antibiotic therapy. In the first case abscess drainage and ventricular external drainage were necessary before sinus and cyst excision. Two months after surgery the two patients were neurologically intact and developping well. Surgery with total excision of dermal sinus and dermoid cyst, even sometimes difficult, must be preferred to the simple abscess drainage and antibiotic therapy. C1 CHU Angers, Serv Neurochirurg, F-49033 Angers, France. CHU Angers, Serv Neuropediatrie, F-49033 Angers, France. RP Hayek, G, CHU Angers, Serv Neurochirurg, F-49033 Angers, France. TC 2 PD MAY PY 2001 VL 47 IS 2-3 PN Part 1 BP 123 EP 127 UT ISI:000169112600006 ER PT J AU Resbeut, M Fondrinier, E Fervers, B Haie-Meder, C Bataillard, A Lhomme, C Asselain, B Basuyau, JP Bremond, A Castaigne, D Dubois, JB Houvenaeghel, G Lartigau, E Leblanc, E Sastre-Garaud, X Ternier, F Guastalla, JP Chauvergne, J TI Carcinoma of the cervix SO BRITISH JOURNAL OF CANCER C1 Inst J Paoli I Calmettes, F-13009 Marseille, France. Ctr Paul Papin, Angers, France. Ctr Leon Berard, F-69373 Lyon, France. FNCLCC, Paris, France. Inst Gustave Roussy, Villejuif, France. Inst Curie, Paris, France. Ctr Henri Becquerel, F-76038 Rouen, France. Ctr Val dAurelle Paul Lamarque, Montpellier, France. Ctr Oscar Lambret, F-59020 Lille, France. Inst Bergonie, Bordeaux, France. RP Resbeut, M, Inst J Paoli I Calmettes, F-13009 Marseille, France. TC 2 PD MAY PY 2001 VL 84 SU Suppl. 2 BP 24 EP 30 UT ISI:000169105700007 ER PT J AU Gallais, S de Crescenzo, MAP Laval-Martin, DL TI Characterization of soluble calcium calmodulin-dependent and -independent NAD(+) kinases from Avena sativa seeds SO AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY DE Avena sativa; calmodulin; dormancy; NAD(+) kinase ID ACHLOROPHYLLOUS ZC MUTANT; PYRIDINE-NUCLEOTIDES; ENHANCED ACTIVITY; EUGLENA-GRACILIS; GIBBERELLIC-ACID; DORMANT SEEDS; ROOT-TIPS; PYROPHOSPHATE; PLANTS; GERMINATION AB Two NAD(+) kinase isoforms were separated from Avena sativa L. embryo extracts from dormant and after-ripened seeds by size-exclusion chromatography: a 410-kDa Ca2+-calmodulin (CaCam)-independent NAD(+) kinase was observed in both dormant and after-ripened seeds and a 63-kDa CaCam-dependent isoform was found to be abundant only in embryos of after-ripened Avena seeds. The characterization of these two isoforms showed differences in Michaelis-Menten constant (K-m) values and enzymatic mechanisms, as well as distinct sensitivities to inhibitors, oxidants, reductants and CaCam inhibitors. It is therefore proposed that the 410-kDa isoform could be a `housekeeping' enzyme with K-m values corresponding to the intracellular concentrations of ATPMg and NAD(+). We speculate that the 63-kDa CaCam-dependent NAD(+) kinase, possessing a low K-m, would be mainly involved in the adaptation and response of A. sativa to environmental signals or stresses through changes of redox potential and/or calcium signalling pathways. C1 Univ Angers, UFR Sci, Grp Biochim & Biol Mol Vegetales, EA 917, F-49045 Angers 01, France. RP de Crescenzo, MAP, Univ Angers, UFR Sci, Grp Biochim & Biol Mol Vegetales, EA 917, 2 Blvd Lavoisier, F-49045 Angers 01, France. TC 2 PY 2001 VL 28 IS 5 BP 363 EP 371 UT ISI:000168995100003 ER PT J AU Rosato, R Gerland, K Jammes, H Bataille-Simoneau, N Segovia, B Mercier, L Groyer, A TI The IGFBP-3 mRNA and protein levels are IGF-I-dependent and GH-independent in MG-63 human osteosarcoma cells SO MOLECULAR AND CELLULAR ENDOCRINOLOGY DE IGFBP-3; osteosarcoma cells; gene expression; insulin-like growth factor-I; growth hormone; growth hormone receptor; actinomycin-D ID GROWTH-FACTOR-I; FACTOR-BINDING PROTEIN-3; HUMAN BONE-CELLS; OSTEOBLAST-ENRICHED CULTURES; OSTEOGENIC PROTEIN-1; HORMONE RESPONSE; GENE-EXPRESSION; RECEPTOR GENE; INSULIN; PROLIFERATION AB Growth Hormone (GH), Insulin-like Growth Factors (IGFs) and IGF-Binding Proteins which modulate the IGFs' bioavailability (e.g. IGFBP-3, -4, -5), are essential regulators of bone remodeling. In this study, MG-63 human osteosarcoma cells were used as a model system to investigate the mechanism(s) whereby IGF-I and GH control IGFBP-3 gene expression. Physiological concentrations of IGF-I (1-20 nM) induced a dose-dependent increase in the steady-state amount of IGFBP-3 mRNA (maximal stimulation: similar to 9-10-fold). This increase was delectable 3 h after the onset of IGF-I treatment, was enhanced over a 24 h period, then plateaued until at least 30 h. Consistently, a dose-dependent increase in IGFBP-3 secretion (similar to 40-50-fold at IGF-I concentrations greater than or equal to 16 nM) was observed by western ligand- and immune-blot analysis of MG-63 cells conditioned medium, and its time course was similar to that observed for IGFBP-3 transcripts. IGFBP-3 mRNA stability (t(1/2) similar to 20 h) was identical in the presence or absence of IGF-I treatment. By contrast, human (h) GH treatment (24-72 h) of MG-63 cells did not increase IGFBP-3 secretion in the conditioned medium. Ectopic expression of recombinant rat GH-R resulted in hGH-enhanced expression of GH-responsive reporter gene constructs, but did not increase endogenous IGFBP-3 gene expression, suggesting that the GH unresponsiveness was not only due to the very low level of GH binding sites at the plasma membrane level. Altogether, these results support the conclusions that in MG-63 cells (i) transcriptional rather post-transcriptional mechanisms are involved in the IGF-I-induced increase of IGFBP-3; (ii) the abundance of GH-R is very low at the plasma membrane level; (iii) the dowstream GH-signaling cascade is fully functional in this human osteosarcoma cell line; and (iv) the endogenous IGFBP-3 gene is not responsive to hGH in human MG-63 osteosarcoma cells. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved. C1 Hop St Antoine, INSERM, U142, F-75571 Paris 12, France. UFR Sci Med & Pharmaceut, LHEA, F-49045 Angers, France. INRA, Mol Endocrinol Lab, F-78352 Jouy En Josas, France. Fac Med Xavier Bichat, INSERM, U327, F-75870 Paris 18, France. RP Groyer, A, Fac Med Xavier Bichat, INSERM, U327, 16 Henri Huchard,BP416, F-75870 Paris 18, France. TC 2 PD APR 25 PY 2001 VL 175 IS 1-2 BP 15 EP 27 UT ISI:000168692000003 ER PT J AU Hambli, R Potiron, A Guerin, F Dumon, B TI Numerical pressure prediction algorithm of superplastic forming processes using 2D and 3D models SO JOURNAL OF MATERIALS PROCESSING TECHNOLOGY DE superplastic forming; pressure algorithm control; finite element; experiment AB The paper describes a finite element model allowing for the prediction in a rapid way the optimum pressure cycle law, the deformed shapes, the distributions of the strain rate and the evolution of the thickness during superplastic forming processes. During the calculation, a pressure-cycle control algorithm is used in the analysis which keeps track of the maximum strain rate sensitivity index m, and adapts the pressure law applied in order to approach as nearly as possible the optimal pressure time history law. The purpose is not to follow the target exactly but obtain a practical. pressure time history in a low computation cost time. To compare the performance of 2D and 3D approaches, two analyses have been performed using 2D and 3D finite element modeling. The numerical results are compared with the experimental ones to verify the validity of the pressure algorithm control. (C) 2001 Elsevier Science B.V. All rights reserved. C1 ISTIA, LASQUO, Upres JE 2039, F-49000 Angers, France. ENSAM, LPMI, F-49035 Angers, France. RP Hambli, R, ISTIA, LASQUO, Upres JE 2039, 62 Ave Notre Dame du Lac, F-49000 Angers, France. TC 2 PD MAY 3 PY 2001 VL 112 IS 1 BP 83 EP 90 UT ISI:000168575300013 ER PT J AU Vuillemin, E Oberti, F Cales, P TI Prevention of esophageal varicose vein rupture SO PRESSE MEDICALE ID ENDOSCOPIC VARICEAL LIGATION; CHRONIC LIVER-DISEASE; LONG-TERM SURVIVAL; PORTAL-HYPERTENSION; CIRRHOTIC-PATIENTS; BLOOD-FLOW; ALCOHOLIC CIRRHOSIS; RANDOMIZED TRIAL; PHARMACOLOGICAL TREATMENT; HEPATIC-ENCEPHALOPATHY AB Primary prevention: Preventive measures against esophageal varicose vein bleeding are needed for grade Z risk resulting from the presence of large varix. Medical or endoscopic methods can be used. Useful drugs: With beta-blockers the incidence of a first bleeding episode is significantly reduced and mortality is almost significantly reduced. Three controlled trials have demonstrated that a nitrate derivative beta-blocker combination improves the efficacy of beta-blockers in terms of reduced bleeding. Nitrates can be prescribed alone in case of a contraindication for beta-blockers but the efficacy is poorly demonstrated. Sclerotherapy: Both incidence of first bleeding episode and mortality are significantly reduced after sclerotherapy. Variable results have however been reported and sclerotherapy is probably only effective in high-risk patients; the consensus conference has not recommended its use. On the contrary, endoscopic ligature is probably useful since 5 preliminary studies have demonstrated a significant effect on first bleeding and mortality. Its relative contribution compared with propranolol remains to be demonstrated. Recommendations: The first intention therapy should rely on betablockers or nitrates in case of a contraindication or adverse effect. Endoscopic ligature can be discussed in high-risk patients. The beta-blocker-nitrate combination and the beta-blocker-endoscopic treatment combination cannot be recommended at the present time, Secondary prevention: Meta-analyses have shown that beta-blockers or sclerotherapy significantly reduce the incidence of recurrent bleeding and mortality Sclerotherapy significantly reduces the risk of recurrent bleeding compared with beta-blockers although there is no difference in mortality and the incidence of complications is significantly greater with sclerotherapy Associating the two methods can significantly reduce recurrent bleeding but has no effect on mortality. Elastic ligature is significantly more effective in terms of recurrent bleeding and mortality or complication rate. Transjugular intrahepatic portosystemic shunt (TIPS) significantly reduces the incidence of recurrent bleeding but has no effect on mortality and the risk of hepatic encephalopathy is increased. Practical attitude: Elastic ligature can be proposed as a first intention treatment. Sclerotherapy is useful to eradicate varix after size reduction by ligature and to prevent recurrence, in case of failure, a TIPS or surgical shunt can be discussed. Liver transplantation would be indicated in high-risk patients keeping in mind that digestive tract bleeding is a supplementary argument favoring transplantation. Recommendations: The international consensus group (CFEHTP) recommends elastic ligature or beta-blockers, endoscopic treatment being preferred. TIPS or surgical shunt may be discussed in case of failure. C1 CHU Angers, Serv HepatoGastroenterol, F-49033 Angers 01, France. RP Cales, P, CHU Angers, Serv HepatoGastroenterol, F-49033 Angers 01, France. TC 2 PD APR 14 PY 2001 VL 30 IS 14 BP 703 EP 710 UT ISI:000168397800009 ER PT J AU Prunier, F Delepine, S Victor, J de Gentile, L Moreau, C Laporte, J Dupuis, JM Geslin, P TI Loffler's fibroblastic endocarditis complicating toxocarosis. SO ARCHIVES DES MALADIES DU COEUR ET DES VAISSEAUX AB The authors report a case of Loffler's fibroblastic endocarditis complicating a toxocarosis infection. Parasitic infestation with toxocara canis is usually asymptomatic, but this was a very rare observation of cerebral involvement associated with symptomatic Loffler's endocarditis. This is an unusual form of restrictive cardiac disease constantly accompanied by prolonged hypereosinophilia. In addition to the classical signs of cardiac failure, an acute febrile illness imitating a connective tissue disease may be observed. Echocardiography helps diagnosis by showing endomyocardial fibrosis and adherent thrombosis at one or both ventricular apices. The management of cardiac failure should include, whenever possible, radical treatment of the hypereosinophilia. At an advanced stage, surgical endocardial decortication is the only means of improving symptoms and the prognosis of these patients. C1 CHU Angers, Serv Cardiol, F-49033 Angers 01, France. CHU Angers, Parasitol Lab, F-49033 Angers, France. CHU Angers, Neurol Serv, F-49033 Angers 01, France. RP Prunier, F, CHU Angers, Serv Cardiol, 4 Rue Larrey, F-49033 Angers 01, France. TC 2 PD MAR PY 2001 VL 94 IS 3 BP 226 EP 230 UT ISI:000168194700010 ER PT J AU Coste, F Ney, B Crozat, Y TI Seed development and seed physiological quality of field grown beans (Phaseolus vulgaris L.) SO SEED SCIENCE AND TECHNOLOGY ID SOYBEAN SEED; REPRODUCTIVE DEVELOPMENT; VIGOR; GERMINATION; VIABILITY AB In this paper, we tested how sequential anthesis and pod location in the canopy could be sources of heterogeneity of seed development and seed physiological quality for a determinate bean (Phaseolus vulgaris L.). In order to achieve various micro-climatic conditions during seed development different treatments were performed during two years under field conditions near Angers (France). Pods were chosen to differ either by anthesis date or by location in the canopy and were hand harvested at regular intervals. After moisture measurement on a sub-sample and slow drying, seed quality was assessed by standard germination, conductivity and controlled deterioration tests. During seed filling and desiccation, seed moisture was found to be a good indicator of seed development. During the end of seed filling and the beginning of seed desiccation, seed quality increased as seed moisture decreased and levelled off at about 0.4 g.g(-1) with no differences between years and treatments or between anthesis date and location of pods. Thus, it was concluded that during this phase, seed quality depended only on seed developmental stage. After the end of desiccation, seed Vigour decreased linearly with increasing time from the end of desiccation; rate of deterioration differed between years and treatments but not between anthesis date or location of pods. C1 ESA, Lab Ecophysiol & Agron, F-49007 Angers, France. RP Coste, F, ESA, Lab Ecophysiol & Agron, 55 Rue Rabelais,BP 748, F-49007 Angers, France. TC 2 PY 2001 VL 29 IS 1 BP 121 EP 136 UT ISI:000167962900014 ER PT J AU Teillet, L Gouraud, S Preisser, L Tordjmann, T Morel, A Corman, B TI Glucagon and vasopressin V1a receptor signaling in hepatocytes from aging rats SO MECHANISMS OF AGEING AND DEVELOPMENT DE V1a receptors; glucagon; hepatocytes; aging ID PROTEIN-KINASE-C; ADENYLATE-CYCLASE; LIVER; GLYCOGENOLYSIS; GLYCATION; AGE; DESENSITIZATION; ACCUMULATION; STIMULATION; EXPRESSION AB Glucose tolerance is reduced with age. The relationship between this change in glucose homeostasis and signaling of glucagon and vasopressin V1a receptors was investigated in hepatocytes isolated from 10- and 30-month-old female WAG/Rij rats. Binding capacity of hepatocytes for I-125 glucagon and H-3 vasopressin increased 2- and 1.8-fold, respectively, between 10 and 30 months. Intracellular cAMP accumulation induced by glucagon was 40% greater in,hepatocytes of aging rats than of adults, although EC,, were similar in the two groups. Conversely, phosphodiesterases activity and nucleotides leakage out of the cells were unchanged with age. The rise in intracellular calcium consecutive to the stimulation of Via receptor was comparable in adult and senescent animals. Finally, glucose release by hepatocyte suspensions was greater in senescent than in adult animals in absence as in presence of glucagon. These experiments suggest that increase in glucagon receptor expression and cAMP generation would contribute to the impaired glucose tolerance characteristic of the aging process. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved. C1 CEA Saclay, Serv Biol Cellulaire, F-91191 Gif Sur Yvette, France. Hop St Perine, APHP, F-75016 Paris, France. Univ Paris Sud, INSERM U442, F-91405 Orsay, France. Univ Angers, F-49000 Angers, France. RP Corman, B, CEA Saclay, Serv Biol Cellulaire, F-91191 Gif Sur Yvette, France. TC 2 PD APR 15 PY 2001 VL 122 IS 4 BP 385 EP 400 UT ISI:000167578000003 ER PT J AU Richir, S Taravel, B Samier, H TI Information networks and technological innovation for industrial products SO INTERNATIONAL JOURNAL OF TECHNOLOGY MANAGEMENT DE internet; engineering design; innovation; toy; electronic commerce AB This article is illustrated by factual industrial cases and shows how influential the generalization of information digital exchange is on the innovation process regarding companies, from engineering design to commercialization of industrial products. Computer networks, either internal (Intranet), 'private' external (Extranet) or 'public' external (Internet) make a 'digital chain' of information exchange which disrupts companies' innovation process. This 'digital engineering design process' allows quicker innovation in a more creative way and favours direct commercialization of industrial products which may be kept virtual (in a digital form) throughout the design process. It allows for design as well as marketing of these industrial products thanks to electronic commerce. Thus toy manufacturers are asking themselves about the relevance of selling their products directly through the internet without any commercial retailer. C1 Univ Angers, ISTIA Innovat Lab, F-49000 Angers, France. RP Richir, S, Univ Angers, ISTIA Innovat Lab, 62 Ave ND Du Lac, F-49000 Angers, France. TC 2 PY 2001 VL 21 IS 3-4 BP 420 EP 427 UT ISI:000167346300016 ER PT J AU Grandidier, B Nys, JP Stievenard, D Krzeminski, C Delerue, C Frere, P Blanchard, P Roncali, J TI Effect of alkyl substituents on the adsorption of thienylenevinylene oligomers on the Si(100) surface SO SURFACE SCIENCE DE molecule-solid reactions; surface chemical reaction; scanning tunneling microscopy; silicon ID SCANNING-TUNNELING-MICROSCOPY; SILICON(001) SURFACE; SI(001); SI AB The adsorption of thienylenevinylene oligomers on the Si(100) surface has been investigated using scanning tunneling microscopy. The mode of substitution of the thiophene ring exerts a strong influence on the adsorption configurations and the images of the oligomer based on 3,4-dihexyl thiophene are highly voltage dependent. We discuss the influence of the alkyl chains on the adsorption process and on the appearance of the molecules in the scanning tunneling microscopy images. (C) 2001 Elsevier Science B.V. All rights reserved. C1 CNRS, Inst Elect & Microelect Nord, Dept ISEN, UMR 8520, F-59046 Lille, France. Univ Angers, CNRS, UMR 6501, F-49045 Angers, France. RP Grandidier, B, CNRS, Inst Elect & Microelect Nord, Dept ISEN, UMR 8520, 41 Bd Vauban, F-59046 Lille, France. TC 2 PD FEB 10 PY 2001 VL 473 IS 1-2 BP 1 EP 7 UT ISI:000167083400001 ER PT J AU Hunault-Berger, M Rachieru, P Ternisien, C Jardel, H Zandecki, M Boasson, M Ifrah, N TI Acquired von Willebrand disease secondary to lymphoproliferative syndromes: 6 cases SO PRESSE MEDICALE ID VONWILLEBRAND-DISEASE; MONOCLONAL GAMMOPATHY; MULTIPLE-MYELOMA; FACTOR-VIII; INTRAVENOUS IMMUNOGLOBULIN; GLYCOPROTEIN IB; PLASMA-CELLS; PROTEOLYSIS; IMMUNOADSORPTION; DESMOPRESSIN AB OBJECTIVE: Acquired von Willebrand disease occurs in patients with or without cutaneous and mucosal bleeding who have no personal or family history of the disease. The clinical course of these patients is poorly known due to the rarity of acquired von Willebrand factor (vWF) deficit. We conducted this study to assess the clinical course of acquired vWF deficit secondary to lymphoproliferative syndromes. PATIENTS AND METHODS: We report the clinical course of acquired von Willebrand disease in 6 patients with monoclonal gammapathy of undetermined significance, multiple myeloma, chronic lymphoid leukemia, Wadenstom's macroglobulinemia, or lymphoma who were followed for 1 to 1 1 years. RESULTS: Acquired von Willebrand disease was suspected in nonthrombocytopenic patients with a lymphoproliferative syndrome who developed a hemorrhagic syndrome. The VWF anomaly was symptomatic in 4 of 6 patients at diagnosis. Patients were given symptomatic treatment with VWF replacement therapy as needed and specific treatment for their lymphoproliferative syndrome. Administration of DDAVP was sufficient in 3 out of 4 patients to allow invasive procedures but was unable to control digestive ulcer bleeding that required infusion of factor VIII-VWF concentrate. For 2 patients, chemotherapy was initiated due to threatening massive hemorrhage. The result was spectacular The 4 other patients have been asymptomatic without treatment for 3, 5, 6 and 11 years during which time their lymphoproliferative syndrome has been quiescent. CONCLUSION: The clinical features and laboratory findings are similar in patients with congenital or acquired von Willebrand disease, but specific and etiologic chemotherapy is indicated for patients with acquired disease. C1 CHU Angers, Serv Malad Sang, F-49033 Angers 01, France. CHU Angers, Hematol Lab, F-49033 Angers, France. CH Vannes, Serv Med Interne, Vannes, France. RP Ifrah, N, CHU Angers, Serv Malad Sang, 4 Rue Larrey, F-49033 Angers 01, France. TC 2 PD FEB 10 PY 2001 VL 30 IS 5 BP 209 EP 212 UT ISI:000167052900002 ER PT J AU Gillet, P TI Flabelligena amoureuxi new genus, new species (Polychaeta : Acrocirridae) from Crozet Islands (Indian Ocean) SO BULLETIN OF MARINE SCIENCE AB A new species of Annelida: Polychaeta, Flabelligena amoureuxi, family Acrocirridae Banse is described from the Crozet Islands (Indian Ocean). Specimens were collected during the Oceanographic Expedition Marion Dufesne MD/08 BENTHOS in 1974. Flabelligena amoureuxi is compared with the other species of the genus Flabelligena and the genus Flabelligera Hartman, 1965. C1 Inst Ecol Appl, Dept Sci Vie & Terre, UCO, F-49008 Angers 01, France. RP Gillet, P, Inst Ecol Appl, Dept Sci Vie & Terre, UCO, 3 Pl Andre Leroy,BP 808, F-49008 Angers 01, France. TC 2 PD JAN PY 2001 VL 68 IS 1 BP 125 EP 131 UT ISI:000166940200012 ER PT J AU Kityk, IV Jakubczyk, E Sahraoui, B Phu, XN TI Photoinduced optical second-harmonic generation in Fe-Co-Si-B metallic spin glasses SO JOURNAL OF OPTICS A-PURE AND APPLIED OPTICS DE photoinduced phenomena; optical second harmonic generation; spin glasses ID INDUCED 2ND-HARMONIC GENERATION; SPECTROSCOPY; ABSORPTION; INTERFACE; SURFACES; FILMS AB For the first time the second-order nonlinear optical effect has been observed in metallic spin glasses. Photoinduced second-harmonic generation (PISHG) in reflected light was used as a particular nonlinear optical method. We have varied the degree of long-range spin ordering detected by magnetoresistance measurements. PISHG is measured immediately after thermotreatment using thermoannealing (up to 823 K) of Fe18Co60Si9B13 glass. As a photoinducing beam a Q-switched nitrogen laser (lambda = 337 nm) was used. A YAG:Nd pulse laser (W = 30 MW, tau = 30 ps, and frequency repetition of about 12 Hz) was applied as a probe. Only the presence of the photoexcitation stimulates the appearance of the PISHG with a value up to 0.128 pm V-1. The influence of the surface, phase matching conditions, fluorescence etc, is discussed. We have revealed an almost linear correlation between the degree of spin ordering and the PISHG output signal for different gaseous media. Molecular dynamics geometry optimization and self-consistent quantum chemical simulations have been performed. The observed phenomenon can be explained by contributions of long-range-ordered spins to electronic dipole moments determining second-order nonlinear optical susceptibilities. C1 Czestochowa WSP, Inst Phys, PL-42201 Czestochowa, Poland. Univ Angers, Lab POMA, Angers, France. RP Kityk, IV, Czestochowa WSP, Inst Phys, Al Armii Krajowej 13-15, PL-42201 Czestochowa, Poland. TC 2 PD JAN PY 2001 VL 3 IS 1 BP 39 EP 44 UT ISI:000166879000008 ER PT J AU Rouge-Maillart, C Tuech, JJ Pessaux, P Riche, P Penneau, M TI Patient information: the current situation in France SO PRESSE MEDICALE AB The Conseil d'Etat, the supreme jurisdiction on legislative matters in France, rendered its decree on January 5, 2000, founding its decision on jurisprudence established in 1997 and 1998 by the supreme Court of Appeals. In accordance with this decision, physicians have a legal obligation to inform patients of all possible risks, including very exceptional risks. The information may be given to the patient in any appropriate form. Proof that information was delivered to the patient is incumbent upon the physician. When proof of information delivery is provided, any injury compensation can only be awarded on the grounds of ill-fate. We conducted an objective review of the jurisprudence on patient information and report the three basic aspects observed in the current situation in France. In application of the Court of Appeals judgments of February 25, 1997 and October 14, 1997, proof of delivery of information to the patient is incumbent upon the physician. The question is whether the physician must retain written documents as necessary proof against future claims. The answer to this question is not straightforward. A written document is not the only proof accepted by the court and could even be of debatable legal value if used inappropriately. The solution retained by the Conseil d'Etat is a good example. The real debate concerns the information content It now appear; that the physician is required to inform his/her patient of all risks susceptible of influencing the patient's decision, particularly serious or life-threatening risks, but also, and most certainly, risks that in the past have been considered frequent but benign. Finally, the judges recall that failure to provide information does not in itself assert the physician's civil responsibility, proof of real damage is also needed. But the reality of damage (ill-fate) depends on the reality of the choice open to the patient had he/she been informed. And the true nature of the choice open to the patient is simply the expression of the dispensable or indispensable nature of the envisaged act. One could say the old adage primum non nocere is making a comeback. C1 CHU Angers, Serv Chirurg Viscerale, F-49033 Angers 01, France. RP Rouge-Maillart, C, CHU Angers, Serv Urgences, 4 Rue Larrey, F-49033 Angers 01, France. TC 2 PD JAN 20 PY 2001 VL 30 IS 2 BP 68 EP 72 UT ISI:000166685900009 ER PT J AU Nail, S Robert, R Dromer, F Marot-Leblond, A Senet, JM TI Susceptibilities of Cryptococcus neoformans strains to platelet binding in vivo and to the fungicidal activity of thrombin-induced platelet microbicidal proteins in vitro SO INFECTION AND IMMUNITY ID ACQUIRED IMMUNODEFICIENCY SYNDROME; ALVEOLAR MACROPHAGES; IN-VITRO; CAPSULE; POLYSACCHARIDE; NEUTROPHILS; INFECTIONS; INHIBITION; MONOCYTES; PHASES AB In this study we investigated the interactions between capsular and acapsular strains of Cryptococcus neoformans and blood platelets. In vivo microscopic observation of blood samples from mice inoculated with C. neoformans yeast cells demonstrated that encapsulated and nonencapsulated yeast cells disappeared quickly from the bloodstream and that platelets were attached solely to yeast cells of the nonencapsulated strains. In vitro we observed that only the acapsular strains were susceptible to the fungicidal activity of thrombin-induced platelet microbicidal proteins. C1 Fac Pharm Angers, Lab Parisitol Mycol, Grp Etud Interact Hote Parasite, F-49000 Angers, France. Inst Pasteur, Unite Mycol, Ctr Natl Reference Mycoses Humaines & Antifong, F-75725 Paris, France. RP Robert, R, Fac Pharm Angers, Lab Parisitol Mycol, Grp Etud Interact Hote Parasite, 16 Blvd Daviers, F-49000 Angers, France. TC 2 PD FEB PY 2001 VL 69 IS 2 BP 1221 EP 1225 UT ISI:000166528700079 ER PT J AU Buldyreva, J Benec'h, S Chrysos, M TI Infrared nitrogen-perturbed NO linewidths in a temperature range of atmospheric interest: An extension of the exact trajectory model SO PHYSICAL REVIEW A ID NITRIC-OXIDE; ARBITRARY SYMMETRY; LINE PARAMETERS; COEFFICIENTS; MOLECULES; BAND AB Infrared NO-N-2 line-broadening coefficients for the fundamental 1-0 band an nb initio computed by means of the exact trajectory model extended to the case of a symmetric top active molecule. As intermolecular potential, the sum model composed of long-range dipole-quadrupole and quadropole-quadrupole interactions as well as short-range atom-atom interactions, is taken. Line-broadening coefficients for both R and P branches of diamagnetic (2)Pi (1/2) and paramagnetic (2)Pi (3/2) sub-bands are reported for various temperatures in the range 163-296 K, thus significantly improving available theoretical results in the common temperature domain, while proposing values for temperatures that as yet have not been studied. The computed results are found to be in good agreement with a bulk of the experimental data. C1 Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, UMR 6136, F-49045 Angers, France. RP Buldyreva, J, Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, UMR 6136, 2 Blvd Lavoisier, F-49045 Angers, France. EM jeanna.buldyreva@univ-angers.fr TC 2 PD JAN PY 2001 VL 6301 IS 1 AR 012708 DI ARTN 012708 UT ISI:000166383000061 ER PT J AU Portet, D Denizot, B Rump, E Hindre, F Le Jeune, JJ Jallet, P TI Comparative biodistribution of thin-coated iron oxide nanoparticles TCION: Effect of different bisphosphonate coatings SO DRUG DEVELOPMENT RESEARCH DE bone targeting; tissular diffusion; MPS; vascular remanence; MRI ID COMPLEMENT ACTIVATION; CONTRAST AGENTS; BONE-MARROW; ADSORPTION; RESONANCE; PARTICLES; LIPOSOMES; MECHANISM; POLYMERS; PROTEIN AB Because the nature of their coatings influences the biodistribution of nanocolloids, five different bisphosphonates bearing OH, NH2, NMe2, and N+Me-3 groups were evaluated in vivo. Fe-59-labeled iron cores were coated by the different molecules and tested by intravenous injection to healthy adult male Wistar rats. The initial phase was estimated with Fe-59- and Tc-99m-labeled nanoparticles biodistribution. The different coatings do not change hydrodynamic radius (similar to12 nm) and relaxivities. The negative surface charge is half for particles coated with bisphosphonates bearing quaternary ammonium compared to those bearing a hydroxyl function. Nanoparticle vascular initial half disappearance time range between 25 and 39 min, with hepatic capture between 50 and 80% ID at 18 h. Bones and muscles fix globally around 35 % ID at 18 h, with high concentrations in the mineral bones. Hydroxy-bisphosphonates and quaternary ammonium-bisphosphonate-coated nanoparticles are the most efficient for blood remanence, weak liver capture, and bone targeting. (C) 2002 Wiley-Liss, Inc. C1 Univ Angers, Inst Biol, Fac Med, UPRES EA 2169,INSERM,ERITM 0104, F-49045 Angers, France. Aventis CropSci, Lyon, France. RP Denizot, B, Univ Angers, Inst Biol, Fac Med, UPRES EA 2169,INSERM,ERITM 0104, 10 Rue Bocquel, F-49045 Angers, France. EM denizot@univ-angers.fr TC 1 PD DEC PY 2001 VL 54 IS 4 BP 173 EP 181 UT ISI:000174167800001 ER PT J AU Bruneau, S Bruhat, C Lagarce, L Laine-Cessac, P TI Retrospective study of adverse drug reactions in the elderly: experience of a regional centre of pharmacovigilance SO THERAPIE DE adverse drug reactions; elderly; pharmacovigilance ID IATROGENIC PATHOLOGY; HOSPITALIZATION AB Cases of adverse drug reactions which occurred in the elderly, and were notified to the Regional Centre of Phamacovigilance of Angers between 1995 and 1998, were analysed in two subgroups: from 75- to 84-years-old and older than 84 years. Among the 263 cases recorded in the elderly, there was no significant difference between the 75- to 84-year-old patients (180 cases) and the patients older than 84 years (83 cases) concerning past medical history, severity of effects or medication. Side-effects in patients over 74 were mainly cutaneous, haematological and neuropsychiatric, involving in decreasing order of frequency cardiovascular, neuropsychiatric, antibiotic, analgesic and anti-inflammatory drugs. In comparison with patient,, under 75, adverse drug reactions are more frequent and more serious, notably overdose and drug interactions, in elderly people. The occurrence of some avoidable side-effects justifies the strengthening of information about therapeutic safety in the elderly. C1 CHU Angers, Pharmacol Lab, Ctr Reg Pharmacovigilance Angers, F-49033 Angers 01, France. RP Laine-Cessac, P, CHU Angers, Pharmacol Lab, Ctr Reg Pharmacovigilance Angers, F-49033 Angers 01, France. TC 1 PD NOV-DEC PY 2001 VL 56 IS 6 BP 785 EP 791 UT ISI:000173877300026 ER PT J AU Regenet, N Tuech, JJ Pessaux, P Aube, C Rousselet, MC Arnaud, JP TI Pancreatic arteriovenous malformation associated with duodenal ulcer and H.Pylori infection SO INTERNATIONAL JOURNAL OF PANCREATOLOGY DE arteriovenous malformation; pancreas; duodenal ulcer; H.Pylori ID COLOR DOPPLER ULTRASONOGRAPHY; DIAGNOSIS; PYLORI AB Pancreatic arteriovenous malformation (PAVM) is a rare condition that may cause duodenal ulcer. A 36-yr-old man with PAVM associated with duodenal ulcer and H. Pylori infection is described. The patient had recurrent episode of upper abdominal pain despite healed ulcer and H. Pylori eradication. The preoperative diagnosis was confirmed by computed tomography and the patient was treated with a pancreatoduodenectomy. Histological examination of the resected pancreas revealed a pancreatic arteriovenous malformation involving the adjacent duodenal wall. C1 CHU Angers, Dept Visceral Surg, F-44033 Angers, France. CHU Angers, Dept Radiol B, F-44033 Angers, France. CHU Angers, Dept Histopathol, F-44033 Angers, France. RP Regenet, N, CHU Angers, Dept Visceral Surg, 4 Rue Larrey, F-44033 Angers, France. TC 1 PD MAR PY 2001 VL 29 IS 2 BP 113 EP 116 UT ISI:000173757400007 ER PT J AU Bellec, V Kaassis, M Burtin, P Arnaud, JP Aube, C Boyer, J TI Acute pancreatis by pancreaticogastric anastomosis stenosis three years after pancreaticoduodenectomy SO GASTROENTEROLOGIE CLINIQUE ET BIOLOGIQUE C1 CHU Angers, Serv Hepatogastroenterol, F-49033 Angers 01, France. CHU Angers, Serv Chirurg Digest, F-49033 Angers, France. CHU Angers, Serv Radiol, F-49033 Angers 01, France. RP Bellec, V, CHU Angers, Serv Hepatogastroenterol, 4 Rue Larrey, F-49033 Angers 01, France. TC 1 PD DEC PY 2001 VL 25 IS 12 BP 1119 EP 1119 UT ISI:000173627800015 ER PT J AU Tuech, JJ Faure, JP Pessaux, P Richert, JP Regenet, N Carretier, M Arnaud, JP TI Surgical management of pancreatic metastases from renal cell carcinoma SO GASTROENTEROLOGIE CLINIQUE ET BIOLOGIQUE C1 CHU Angers, Dept Chirurg Digest, F-49000 Angers, France. CHU Poitiers, Dept Chirurg Digest, F-86021 Poitiers, France. RP Tuech, JJ, CHU Angers, Dept Chirurg Digest, 4 Rue Larrey, F-49000 Angers, France. TC 1 PD DEC PY 2001 VL 25 IS 12 BP 1120 EP 1121 UT ISI:000173627800016 ER PT J AU Robert, JJ Ginies, JL Huet, F TI Diabetes, osteoporosis, articular manifestations SO ARCHIVES DE PEDIATRIE ID BONE-MINERAL DENSITY; CYSTIC-FIBROSIS; BODY-COMPOSITION; YOUNG-ADULTS; CHILDREN; PAMIDRONATE; ARTHROPATHY; FRACTURES; ARTHRITIS; KYPHOSIS C1 Hop Enfants Bocage, Serv Pediat 1, F-21034 Dijon, France. Hop Necker Enfants Malad, F-75015 Paris, France. CHU Angers, Dept Pediat, F-49100 Angers, France. RP Huet, F, Hop Enfants Bocage, Serv Pediat 1, F-21034 Dijon, France. TC 1 PD DEC PY 2001 VL 8 SU Suppl. 5 BP 894S EP 900S UT ISI:000173441200008 ER PT J AU Kityk, IV Bragiel, P Piasecki, M Sahraoui, B Hudhomme, P Gorgues, A TI Phase transition in C-60-2-thioxo-1,3-dithiole cycloadduct SO PHASE TRANSITIONS DE phase transition; fullerene derivatives; quadratic electrooptics effect AB Quadratic electrooptic coefficient (QEOC) measurements have been made for fullerene C-60 and its derivative C-60-2-thioxo-1,3-dithiole. An anomaly in C-p has been revealed at about -60degreesC that corresponds to the appearance of a structural: phase transition. Correlation with a temperature anomaly in the QEOC is revealed. This leads to the conclusion that the QEOC for fullerene depends mainly on vibrational and rotational contributions, The contribution arising from charge distribution asymmetry is most important in the fullerene derivative. The observed temperature anomalies of the QEOC result in the possibility of using the QEOC as a sensitive tool for observing low-temperature phase transitions in the fullerene derivatives. C1 Pedag Univ Czestochowa, Inst Phys, PL-42200 Czestochowa, Poland. Univ Angers, Lab POMA, CNRS, EP 130 2, F-49045 Angers, France. Univ Nantes, CNRS, UMR 6513, Organ Synth Lab, F-443322 Nantes, France. RP Kityk, IV, Pedag Univ Czestochowa, Inst Phys, Al Armii Krajowej 13-15, PL-42200 Czestochowa, Poland. TC 1 PY 2001 VL 74 IS 4 PN Part A BP 347 EP 352 UT ISI:000173365600002 ER PT J AU Mougel, C Teyssier, S D'Angelo, C Groud, K Neyra, M Sidi-Boumedine, K Cloeckaert, A Peloille, M Baucheron, S Chaslus-Dancla, E Jarraud, S Meugnier, H Forey, F Vandenesch, F Lina, G Etienne, J Thioulouse, J Manceau, C Robbe, P Nalin, R Briolay, J Nesme, X TI Experimental and theoretical evaluation of typing methods based upon random amplification of genomic restriction fragments (AFLP) for bacterial population genetics SO GENETICS SELECTION EVOLUTION DE AFLP; genomospecies; genetic distance; infraspecific diversity ID FIELD GEL-ELECTROPHORESIS; PSEUDOMONAS-SYRINGAE; ESCHERICHIA-COLI; DNA; STRAINS; IDENTIFICATION; RELATEDNESS; DIVERSITY; PCR; STENOTROPHOMONAS AB The reliability and the level of taxonomic resolution of the amplified fragment length polymorphism (AFLP) method were evaluated with species of pathogenic bacteria involved in human, animal and plant diseases. The method was found to be very versatile as it can be adapted to the individual genome constraints of all tested species. The calculation of a genetic distance d corresponding to the average dissimilarity between actual overall genome sequences was proposed for comparing AFLP data. Bacterial models showed clearly different patterns between strains belonging to different genomic species, while patterns were clearly similar within a given species. The threshold which distinguishes between inter and infra-specific distances indicates a critical overall genome diversity of about 14% (d = 0.14). AFLP had more resolution power than serology, phage typing, PFGE and restriction analysis of ribosomal intergenic spacers. In the latter case, regression analysis showed that PCR-RFLP of ribosomal intergenic spacers can only be used to differentiate bacteria which have at least 3.4% (d = 0.034) nucleotide differences between their respective genomes. Finally, an improved procedure using newly developed software was also proposed in order to standardize the capture of reliable data and their numeric treatment for the future development of AFLP data bases. C1 Univ Lyon 1, CNRS, UMR 5557, INRA, F-69622 Villeurbanne, France. IRD, F-69622 Villeurbanne, France. INRA, Stn Pathol Aviaire, F-37380 Nouzilly, France. Univ Lyon 1, Fac Med Laennec, EA1655, Dept Microbiol Mol & Med, F-69372 Lyon 08, France. Univ Lyon 1, CNRS, UMR 5558, Lab Biometr Genet & Biol Populat, F-69622 Villeurbanne, France. Univ Angers, INRA, INH, UMR PaVe, F-49071 Beaucouze, France. Univ Lyon 1, LIBRAGEN, F-69622 Villeurbanne, France. RP Nesme, X, Univ Lyon 1, CNRS, UMR 5557, INRA, 43 Bd 11 Novembre 1918, F-69622 Villeurbanne, France. TC 1 PY 2001 VL 33 SU Suppl. 1 BP S319 EP S338 UT ISI:000173270700019 ER PT J AU Barille, R Rivoire, G TI Spatial solitons in nonlinear liquid waveguides SO PRAMANA-JOURNAL OF PHYSICS DE nonlinear waveguide; stimulated Rayleigh wing scattering; soliton; polarization switch ID RAYLEIGH WING SCATTERING; PLANAR WAVE-GUIDE; LASER-BEAMS; PROPAGATION AB Spatial solitons are studied in a planar waveguide filled with nonlinear liquids. Spectral and spatial measurements for different geometries and input power of the laser beam show the influence of different nonlinear effects as stimulated scatterings on the soliton propagation and in particular on the beam polarization. The stimulated scattering can be used advantageously to couple the two polarization components. This effect can lead to multiple applications in optical switching. C1 Univ Angers, CNRS, Lab Proprietes Opt Mat & Applicat, F-49016 Angers, France. RP Barille, R, Univ Angers, CNRS, Lab Proprietes Opt Mat & Applicat, 4 Blvd Lavoisier,BP 2018, F-49016 Angers, France. TC 1 PD NOV-DEC PY 2001 VL 57 IS 5-6 BP 1139 EP 1161 UT ISI:000172826000018 ER PT J AU Maloisel, F Guerci, A Vigier, M Blanc, M Berger, M Gardembas, M Legros, L Vuillier, J Tertian, G Michallet, M Dorvaux, V Reiffers, J Djazouli, K Guilhot, J Guilhot, F TI Safety and efficacy of cytarabine ocfosfate (YNKO1) by continuous or intermittent administration, in combination with interferon alfa 2b (IFN) for patients with de novo chronic myeloid leukemia: A phase I study. SO BLOOD C1 Hop Civil, Strasbourg, France. Hop Brabois, Nancy, France. Hop Hotel Dieu, Nantes, France. CH, Chambery, France. CHU, Clermont Ferrand, France. CHU Angers, Angers, France. Hop Archet, Nice, France. Hop Minjo, Besancon, France. APHP, Le Kremlin Bicetre, France. Hop Edouard Herriot, Lyon, France. Hop Bon Secours, Metz, France. CHU Haut Leveque, Pessac, France. Hop Claude Bernard, Poitiers, France. TC 1 PD NOV 16 PY 2001 VL 98 IS 11 PN Part 1 BP 349A EP 349A UT ISI:000172134101477 ER PT J AU Hunault, M Harousseau, JL Delain, M Cahn, JY Pignon, B Witz, F Lamy, T Jouet, JP Desablens, B Caillot, D Berthou, C Guyotat, D Sadoun, A Truchan-Graczyk, M Ifrah, N CA GOELAMS Grp TI Improved outcome of high risk acute lymphoblastic leukemia (ALL) with late high dose therapy. A GOELAMS's trial. SO BLOOD C1 Dept Hematol, Angers, France. TC 1 PD NOV 16 PY 2001 VL 98 IS 11 PN Part 1 BP 803A EP 803A UT ISI:000172134103352 ER PT J AU Salaun, M Charpentier, S TI Rapid analysis of organic and amino acids by capillary electrophoresis: application to glutamine and arginine contents in an ornamental shrub SO JOURNAL OF PLANT PHYSIOLOGY DE amino acids; capillary electrophoresis; Ligustrum; nitrate; organic acids ID ZONE-ELECTROPHORESIS; STORED N; TREES; NITROGEN; REMOBILIZATION; CHROMATOGRAPHY; SEPARATION; EXTRACTS; TISSUE AB Soluble nitrogen-containing compounds in plant extracts are often measured using chromatographic techniques that require not only previous derivatisation and a long analysis time, but are also costly. Two rapid methods were developed for analysing NO3-, organic, and amino acids by capillary electrophoresis (CE). A mixture of NO3-, organic, and amino acids was separated within 7 min, directly from a plant extract with good repeatability and linearity. An alternative method, requiring the previous purification of a plant extract on resin, improved the identification of amino acids in samples with numerous peaks and a low amino acid content. These two methods were used to evaluate the soluble nitrogen-containing compounds in an ornamental shrub (Ligustrum ovalifolium) subjected to three fertigation timetables. Arginine and glutamine were the main compounds observed in the plants during N redistribution. Their contents increased dramatically in autumn. In December, arginine followed an increasing gradient from the roots to the trunk and decreased from the lower to the upper parts of stems. The more fertilisation the plants received, the higher the arginine content. Glutamine content was lowest in the three treatments. In March, arginine and glutamine contents returned to their minimal values. C1 Univ Angers, Inst Natl Hort, INH, SAGAH UMR INRA, F-49045 Angers 01, France. RP Salaun, M, Univ Angers, Inst Natl Hort, INH, SAGAH UMR INRA, 2 Rue Le Norte, F-49045 Angers 01, France. TC 1 PD NOV PY 2001 VL 158 IS 11 BP 1381 EP 1386 UT ISI:000172442500003 ER PT J AU Boucher, V Phu, XN TI Polarization field in optical spatial vector solitons SO OPTICS COMMUNICATIONS AB We report for the first time the experimental observation of polarization field in optical spatial solitons in nonlinear planar waveguide. The measurements have been checked by an improved local method (nonlinear ellipsometry method). The results confirm the uniformity of the polarization field for TE and TM solitons (in 1 + 1D propagation). In the case of mixed TE-TM input polarization, we have pointed out the existence of spatial vector solitons in such nonlinear waveguides. Their output polarization state distribution is uniform in the transverse section. (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, Lab Proprietes Opt Mat & Applicat, F-49045 Angers 01, France. RP Boucher, V, Univ Angers, Lab Proprietes Opt Mat & Applicat, 2 Blvd Lavoisier, F-49045 Angers 01, France. TC 1 PD NOV 15 PY 2001 VL 199 IS 1-4 BP 223 EP 229 UT ISI:000172176300027 ER PT J AU Brun, S Bouchara, JP Bocquel, A Basile, AM Contet-Audonneau, N Chabasse, D TI Evaluation of five commercial sabouraud gentamicin-chloramphenicol agar media SO EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES ID FUNGAL-INFECTIONS; CANDIDA; IDENTIFICATION; ONYCHOMYCOSIS; EPIDEMIOLOGY; PATHOGENS AB The diagnosis of fungal infections relies on the isolation of the causative agent by culture of clinical specimens. Among the different culture media, Sabouraud glucose agar remains the most widely used. The use of commercial culture media is highly recommended as good laboratory practice in clinical microbiology. Therefore, the comparative performance of five different Sabouraud gentamicin-chloramphenicol agar media, available commercially as plates, was investigated. A total of 124 strains encompassing 45 yeasts and 79 filamentous fungi were cultured. Colonies of the dermatophytes (28 strains) and some related keratinophilic fungi (6 strains) were of overall similar appearance or size on all five media. Conversely, all the Aspergillus strains tested (n=17) as well as a few other strains of Hyphomycetes (n=5/18) exhibited important differences in the colour of the colonies. Furthermore, growth of the members of Mucoraceae was also affected, with great differences in the diameter of the colonies observed. In addition, quantitative cultures of the yeasts revealed marked variations in the number of the colonies, or even no growth, for two Candida species, Cryptococcus species, and Trichosporon cutaneum. In conclusion, the only formulation that gave good results with all fungal types tested was the one from Becton Dickinson (France). C1 CHU Angers, Grp Etud Interact Hote Parasite, UPRES EA 3142, Lab Parasitol Mycol, F-49033 Angers 01, France. Fac Med Vandoeuvre Nancy, Lab Parasitol Mycol, F-54505 Vandoeuvre Les Nancy, France. RP Brun, S, CHU Angers, Grp Etud Interact Hote Parasite, UPRES EA 3142, Lab Parasitol Mycol, 4 Rue Larrey, F-49033 Angers 01, France. TC 1 PD OCT PY 2001 VL 20 IS 10 BP 718 EP 723 UT ISI:000172202900005 ER PT J AU Pouliquen, D Foussard, F Tanguy, G Roux, J Malthiery, Y TI Total and structured water in cancer: An NMR experimental study of serum and tissues in DMBA-induced OF1 mice SO CELLULAR AND MOLECULAR BIOLOGY DE water; cancer; NMP; serum; liver; lymphoma ID NUCLEAR MAGNETIC-RESONANCE; PHYTOCHEMICALS; COMPLEXES; VOLUME; STATE; RATS AB Total water and Structured water (fraction of total water which remains unfrozen below the transition point from the semisolid to solid state) were characterized by H-1 NMR relaxometry in the sera and tissues of 3 groups of 30 female mice (C, H and L) receiving a single administration of DMBA and different diets. Mice given the diet H, containing the highest proportion of saturated fatty acids and processed starch, and the lowest phytochemicals content, presented the highest tumor incidence (lymphoma). This allowed 3 subgroups to be defined: subnormal (SN), small (T+) and large tumor (T++). Spin-lattice relaxation times of total water (Tlobs) in the sera and tissues did not significantly differ between C, H and L groups, and SN, T+ and T++ subgroups. In T+ mice, a decrease in the relative amount of structured water was noticed in the serum, liver and heart, while changes in the temperature dependence of the T1 of structured water (T1sw) were observed between -21 degreesC and -42 degreesC. These results suggest a moderate increase in the rotational mobility of structured water molecules in the serum and the heart, and a pronounced decrease in the liver. Likewise, the modification of the T1sw temperature dependence curve's shape tends to confirm the existence of important conformational changes in the macromolecular assemblies, which markedly affect the properties of structured water, especially in the earliest stage of cancer development. C1 INSERM, EMI 00 18, Lab Biochim & Biol Mol, F-49045 Angers, France. Lab Biophys Med, UPRESEA 2169, F-49045 Angers, France. Animalerie Hosp Univ, Fac Med, F-49045 Angers, France. RP Pouliquen, D, INSERM, EMI 00 18, Lab Biochim & Biol Mol, F-49045 Angers, France. TC 1 PD JUL PY 2001 VL 47 IS 5 BP 947 EP 957 UT ISI:000171860500020 ER PT J AU Buldyreva, J Chrysos, M TI Semiclassical modeling of infrared pressure-broadened linewidths: A comparative analysis in CO2-Ar at various temperatures SO JOURNAL OF CHEMICAL PHYSICS ID POTENTIAL-ENERGY SURFACES; CARBON-DIOXIDE; NITROUS-OXIDE; LINE; N-2; BAND; AR; O-2; TRANSITIONS; RELAXATION AB A novel semiclassical approach, which makes use of the exact trajectory implemented within the Robert-Bonamy formalism, is employed for modeling infrared pressure-broadened linewidths. As a prototype, the carbon dioxide molecule perturbed by argon is examined in the temperature range 160-760 K, for which various measurements and computations are available. For a meaningful comparison with previous theoretical works done with both semiclassical and quantum approaches, the ab initio intermolecular potential surface of Parker [J. Chem. Phys. 64, 1668 (1976)] is used. Our values are found to be in agreement with up-to-date experimental data at all temperatures studied. (C) 2001 American Institute of Physics. C1 Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, Unite Mixte Rech UMR 6136, F-49045 Angers, France. RP Buldyreva, J, Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, Unite Mixte Rech UMR 6136, 2 Blvd Lavoisier, F-49045 Angers, France. TC 1 PD OCT 22 PY 2001 VL 115 IS 16 BP 7436 EP 7441 UT ISI:000171719200013 ER PT J AU Pessaux, P Tuech, JJ Arnaud, JP TI Prevention of pancreatic fistulae after surgical resection: a decade of clinical trials SO PRESSE MEDICALE ID RECONSTRUCTION FOLLOWING PANCREATICODUODENECTOMY; CONSECUTIVE PANCREATICODUODENECTOMIES; MALIGNANT DISEASE; RANDOMIZED TRIAL; COMPLICATIONS; OCTREOTIDE; PANCREATICOJEJUNOSTOMY; PANCREATICOGASTROSTOMY; SURGERY; PANCREATODUODENECTOMY AB Prevention of fistula formation Pancreatic fistulae occur in about 10-20% of patients undergoing pancreaticoduodenectomy and are a leading cause of morbidity. We reviewed trials devoted to prevention. Surgical procedures for reduction of pancreatic secretion and modification of the remnant pancreas could be useful as preventive measures. Innovating surgery Several surgical procedures have been proposed to decrease the rate of complications, but none have demonstrated efficacy in a prospective randomized trial. Pancreatogastrostomy (PG) or pancreatojejuneostomy (PJ) have been compared in nine studies: seven retrospective series and two prospective randomized trials. The one trial using a correct randomization method found equivalent early results for PC and PJ. Inhibitors of pancreas secretion Eleven randomized trials have assessed the use of somatostatin or octreotide for the prevention of fistulae after pancreaticoduodenectomy. There has been significant heterogenity in these trials concerning the definition of fistula, dosage of octreotide, starting time and duration of treatment. Six studies have concluded that these drugs are effective while 5 concluded that there is no significant difference. Perspectives There is no convincing argument to affirm the superiority of PC or PJ. For the present, the use of somatostatin or octreotide cannot be recommended in routine practice for the prevention of postoperative complications of pancreatic surgery. C1 CHU Angers, Dept Chirurg Viscerale, F-49033 Angers 01, France. RP Arnaud, JP, CHU Angers, Dept Chirurg Viscerale, 4 Rue Larrey, F-49033 Angers 01, France. TC 1 PD SEP 29 PY 2001 VL 30 IS 27 BP 1359 EP 1363 UT ISI:000171458200011 ER PT J AU Croquet, V Moal, F Oberti, F Roux, J Gallois, Y Cales, P TI Hemodynamic and antifibrotic effects of losartan in portal hypertensive rats. SO HEPATOLOGY C1 Fac Med, Angers, France. TC 1 PD OCT PY 2001 VL 34 IS 4 PN Part 2 Suppl. S BP 257A EP 257A UT ISI:000171224700336 ER PT J AU Hentati, N Pasco, A Fournier, HD Papon, X Mercier, P TI Double hepatomesenteric artery: a rare anatomical variant SO SURGICAL AND RADIOLOGIC ANATOMY DE hepatic pedicle; variants of hepatic arteries; vascular variants; variations ID PANCREATICODUODENAL ARTERY; PANCREAS AB A case of a "double hepatomesenteric artery", i.e. two persisting hepatic arteries arising from the superior mesenteric artery, is reported. Also observed was a lack of systematisation of the pancreaticoduodenal arteries. The variant, which does not appear to have been previously reported in the literature, was observed when dissecting the vessels of the duodenal bulb in a preserved male cadaver injected with neoprene latex. Its embryological origin, as well as the clinical, radiological (diagnostic or interventional) and surgical implications, are discussed. C1 Fac Med, Lab Anat, F-49045 Angers, France. CHU Angers, Hop Larrey, Serv Radiol C, F-49045 Angers, France. RP Mercier, P, Fac Med, Lab Anat, Rue Haute de Reculee, F-49045 Angers, France. TC 1 PD AUG PY 2001 VL 23 IS 4 BP 285 EP 289 UT ISI:000171246400015 ER PT J AU Vialle, R Pietin-Vialle, C Cronier, P Brillu, C Villapadierna, F Mercier, P TI Anatomic relations between the cephalic vein and the sensory branches of the radial nerve: How can nerve lesions during vein puncture be prevented? SO ANESTHESIA AND ANALGESIA AB The cephalic vein of the forearm is often used for IV catheters because of its ease of access for peripheral venous cannulation. But its close relation to the sensory branch of the radial nerve sometimes causes it to be damaged when the vein is cannulated. Our anatomic study conducted on 33 specimens confirmed the risk of nerve lesion. However, it is impossible to define a safe zone, because of the randomly located nerve and vein crossing zones, where the iatrogenic risk of damaging the radial nerve is maximum. We suggest that to avoid incidents, the cephalic vein should be punctured above the emergence of the sensory branch of the radial nerve, e.g., at least 12 cm above the level of the styloid process of the radius. C1 Fac Med, Lab Anat, F-49045 Angers, France. RP Mercier, P, Fac Med, Lab Anat, Rue Haute Reculee, F-49045 Angers, France. TC 1 PD OCT PY 2001 VL 93 IS 4 BP 1058 EP 1061 UT ISI:000171237900047 ER PT J AU Fressinaud, C Vigneron, I Letournel, F Jean, I TI Cytoskeleton abnormalities in axonopathies of unknown aetiology: resemblance with axotomy SO JOURNAL OF NEUROCHEMISTRY C1 Univ Hosp, Dept Neurol, F-49033 Angers, France. Univ Hosp, Cell Biol Lab, UPRES EA 3143, F-49033 Angers, France. TC 1 PD SEP PY 2001 VL 78 SU Suppl. 1 BP 93 EP 93 UT ISI:000170789800331 ER PT J AU Paulin, JP Ride, M Prunier, JP TI Discovery of plant pathogenic bacteria: dispute and controversy between America and Europe. SO COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES DE bacteria; phytopathology AB The demonstration of a bacterial cause of some plant diseases has been claimed few years after it was commonly recognized that bacteria were able to cause diseases of human and animal. Nevertheless. some sharp controversies took place, between German and American specialists (1897-1901). before the existence of bacterial diseases of plants was accepted by all phytopathologists. Nowadays. about 350 bacteria are described, which infect plants: they are pathovars, or subspecies, belonging to 21 genera, Bacterial diseases of plants can be classified into three major categories according to the type of symptoms shown by the infected plant: necrosis and wilt, soft-rot, tumour. The interaction between bacteria and plant cells is usually established from the apoplast, although SOI-ne bacteria are xylem or phloem limited, This interaction involves an original protein secretion system (which is also described in bacteria pathogenic for animals), hydrolytic enzymes (pectinases, cellulases), toxins and/or phytohormones, Bacteria of one group (Agrobacterium) modify the plant metabolism after gene transfer from a plasmid. on the economic and social point of view, these diseases may be limiting factors of some key-productions (rice, cassava). In addition, they play a role in reducing the quality of agricultural products (reduced growth, spots on leaves and fruits). Control of bacterial diseases is limited, It relies usually on a combination of prophylaxy, chemical applications, and use of resistant genotypes. (C) 2001 Academie des sciences/Editions scientifiques et medicales Elsevier SAS. C1 Univ Angers, INRA, INH, UMR,Ctr INRA Angers, F-49071 Beaucouze, France. INRA, DSPPV, F-75338 Paris 07, France. RP Paulin, JP, Univ Angers, INRA, INH, UMR,Ctr INRA Angers, 42 Rue Georges Morel,BP 57, F-49071 Beaucouze, France. TC 1 PD OCT PY 2001 VL 324 IS 10 BP 905 EP 914 UT ISI:000171000100005 ER PT J AU Fanello, S Blay, M Laine-Cessac, P Daryabin, M TI Hypothermia induced by neuroleptics SO REVUE DE MEDECINE INTERNE DE hypothermia; neuroleptics; iatrogenic C1 CHU Angers, Serv Med E, F-49045 Angers, France. RP Fanello, S, CHU Angers, Serv Med E, 1 Rue Larrey, F-49045 Angers, France. TC 1 PD AUG PY 2001 VL 22 IS 8 BP 764 EP 766 UT ISI:000170492500014 ER PT J AU Defaix, A Lapied, B TI Characterization of a novel low-voltage-activated tetrodotoxin-sensitive sodium channel permeable to calcium in insect pacemaker neurosecretory cells SO JOURNAL OF PHYSIOLOGY-LONDON C1 Univ Angers, Neurophysiol Lab, UPRES EA 2647 RCIM, UFR Sci, F-49045 Angers, France. TC 1 PD MAY PY 2001 VL 533 SU Suppl. S BP 54P EP 55P UT ISI:000169224300069 ER PT J AU Kaassis, M Moal, F Veal, N Oberti, F Cales, P TI Predictive factors of response to octreotide for the treatment of portal hypertensive rats SO JOURNAL OF HEPATOLOGY C1 Univ Angers, HIFIH Lab, Angers, France. TC 1 PD APR PY 2001 VL 34 SU Suppl. 1 BP 72 EP 73 UT ISI:000169243400252 ER PT J AU Bernard, C TI Essential oils of three Angelica L. species growing in France. Part II: Fruit oils SO JOURNAL OF ESSENTIAL OIL RESEARCH DE Angelico archangelica ssp archangelica; Angelico archangelica ssp litoralis; Angelico sylvestris var. vulgaris; Angelico heterocarpa; Apiaceae; essential oil composition; beta-phellandrene; limonene AB The essential oils obtained from fruits of three Angelica L. species growing in France were analyzed by, GC/MS. The dominant component in the fruit oils of Angelica archangelica L. and A. heterocarpa Lloyd was beta -phellandrene (64.9-76.0% and 39.0%, respectively), while the major component of A. sylvestris var. vulgaris Ave-Lallemant was limonene (56.1%). One result of this study, was that numerous volatile components found have never before been described as constituents of the three Angelico species examined. The chemical analyses present numerous systematic markers for each species and bring together Angelica archangelica and A. heterocarpa. This study has led to the distinction of two chemotypes and one ecotype of Angelico archangelica subsp.sativa. C1 Inst Univ Technol, Dept Biol Appl, F-49016 Angers, France. RP Bernard, C, Inst Univ Technol, Dept Biol Appl, 4,Bd Lavoisier BP 2018, F-49016 Angers, France. TC 1 PD JUL-AUG PY 2001 VL 13 IS 4 BP 260 EP 263 UT ISI:000170503900016 ER PT J AU Lortholary, A TI Tumor-induced hypercalcemia. A review of the treatment by bisphosphonates SO REVUE DE MEDECINE INTERNE DE hypercalcemia; PTHrP; bisphosphonates ID CANCER-ASSOCIATED HYPERCALCEMIA; HORMONE-RELATED PROTEIN; PARATHYROID-HORMONE; HUMORAL HYPERCALCEMIA; MALIGNANCY; PAMIDRONATE; MANAGEMENT; CLODRONATE; CARCINOMA; AHPRBP AB Introduction. - The incidence of tumor-induced hypercalcemia is between 10 to 20%. New treatments justify this review article. Current knowledge and key points. - Tumor-induced hypercalcemia (half of all hypercalcemia) is divided into two groups: hematological tumors (10%), and solid tumors (90%), with osteolytic hypercalcemia and humoral hypercalcemia of malignancy (HHM. mediators include PTHrP). The two most common causes of tumor-induced hypercalcemia are lung cancer and breast cancer. Tumor-induced hypercalcemia most commonly disturbs gastrointestinal, neurological, renal and cardiovascular functions. These symptoms may be erroneously attributed to the underlying malignancy or its therapy. Prognosis of tumor-induced hypercalcemia is very poor, with median survival being about 3 months. Bisphosphonates have emerged as the standard treatment of tumor-induced hypercalcemia. The intravenous administration of isotonic saline is the first step in the management of tumor-induced hypercalcemia. Specific treatment of cancer remains essential to prevent TIH relapse. Future prospects and projects. - New bisphosphonates have appeared, the most potent known bisphosphonate today is zoledronate. (C) 2001 Editions scientifiques et medicales Elsevier SAS. C1 Ctr Paul Papin, Dept Oncol, F-49000 Angers, France. RP Lortholary, A, Ctr Paul Papin, Dept Oncol, 2 Rue Moll, F-49000 Angers, France. TC 1 PD JUL PY 2001 VL 22 IS 7 BP 648 EP 652 UT ISI:000170218300006 ER PT J AU Demol, F De Backer, MG Levillain, E Sauvage, FX TI A spectroelectrochemical study of the reduction of a Schiff base cryptand SO SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY DE radical anion; spectroelectrochemistry; cryptand; Schiff base; visible and near infrared spectroscopy ID ELECTRIDES; ALKALIDES AB The electrochemical reduction of a bicyclic hexamino Schiff base cryptand 1 (N[(CH2)(2)N=CH (.) meta-C6H4-CH=N(CH2)(2)](3)N) and that of one of its strands 2 ((CH3)2CH (.) N=CH (.) meta-C6H4-CH=N-CH(CH3)(2)) has been studied by visible and near infrared in-situ spectroelectrochemical techniques. These results are in good agreement with those obtained using alkali metals, but in this case the effect of the formation of ion pairs is minimized through the use of tetrabutylammonium cations. It is confirmed that 1 and 1 have the same visible and near IR sptectrum. The spectrum of the products of the electrochemical reduction of 2 is similar to those of 1 or 1(-). (C) 2001 Elsevier Science B.V. All rights reserved. C1 CNRS, UMR 8516, LASIR, HEI, F-59046 Lille, France. CNRS, UMR 6501, IMMO, F-49045 Angers, France. RP De Backer, MG, CNRS, UMR 8516, LASIR, HEI, 13 Rue Toul, F-59046 Lille, France. EM marc.debacker@hei.fr TC 1 PD JUL PY 2001 VL 57 IS 8 BP 1611 EP 1619 UT ISI:000169725600008 ER PT J AU Tuech, JJ Pessaux, P Arnaud, JP TI Portal vein resection in pancreatic head carcinoma - Part 1: Technical considerations SO HEPATO-GASTROENTEROLOGY DE pancreas; portal vein; extended pancreatectomy; pancreaticoduodenectomy; surgery; portal vein resection ID SUPERIOR MESENTERIC VEIN; REGIONAL VASCULAR RESECTION; SURGICAL-TREATMENT; CATHETER BYPASS; CANCER; PANCREATICODUODENECTOMY; ADENOCARCINOMA; RECONSTRUCTION; CONFLUENCE; INVASION AB It has been reported that pancreatic carcinoma invading the superior mesenterico-portal vein confluence can be resected safely. Nunerous techniques have been described for portal vein reconstruction, these procedures are described and discussed by the authors. C1 Angers Univ Hosp, Dept Digest Surg, F-49000 Angers, France. RP Tuech, JJ, Angers Univ Hosp, Dept Digest Surg, 4 Rue Larrey, F-49000 Angers, France. TC 1 PD MAY-JUN PY 2001 VL 48 IS 39 BP 884 EP 887 UT ISI:000169631600066 ER PT J AU Fichou, G TI Group law on neutral component of the Jacobian of a real curve of genus 2 having many real components SO MANUSCRIPTA MATHEMATICA ID ALGEBRAIC-CURVES AB Let C be a real algebraic curve of genus 2 with at least two real components B-1 and B-2. We give an embedding of C into the projective plane, blown up in a point. It allows us to describe geometrically the neutral real component Pic(O)(C)(O) of the Jacobian of C thanks to an isomorphism with the product B-1 x B-2. This induices an explicit geometric description of Pic(O)(C)(O) in the projective plane, where the group law on. B is given by intersection with conics. C1 Univ Angers, Dept Math, F-49045 Angers, France. RP Fichou, G, Univ Angers, Dept Math, 2 Bd Lavoisier, F-49045 Angers, France. TC 1 PD APR PY 2001 VL 104 IS 4 BP 459 EP 466 UT ISI:000169272200005 ER PT J AU Benayoun, S Hantzpergue, JJ Bouteville, A TI Micro-scratch test study of TiN films grown on silicon by chemical vapor deposition SO THIN SOLID FILMS DE coatings; titanium nitride; silicon; surface stress ID STAINLESS-STEEL; NITRIDE LAYERS; ADHESION; COATINGS; TICL4-NH3-H-2; INDENTATION; BARRIER AB TiN thin films (0.095 mum) were deposited on silicon wafers at 800 degreesC by rapid thermal low-pressure chemical vapor deposition from a TiCl4 + NH3 + H-2 gaseous mixture. Micro-scratches were performed using a system equipped with an optical microscope, an acoustic emission sensor and a friction force sensor. Critical loads were determined to evaluate the adhesion of TiN to the substrate. The influence of the indenter state on the critical load was studied. Scanning electron micrographs, electron probe microanalyses and chemical etching of the film were performed to identify and interpret the damage mechanisms. Experimental results were analyzed using the Weibull statistic. It was shown that small particles of TiN were chipped off and formed a third body, which modified the indenter-him contact and contributed to an increase in the range of the critical load. Models previously proposed in the literature, which allow calculation of the work of adhesion from a critical load, are discussed with respect to the damage mechanisms observed. (C) 2001 Elsevier Science B.V. All rights reserved. C1 ENSAM, Lab Procedes Mat Instrumentat, F-49035 Angers, France. RP Benayoun, S, ENSAM, Lab Procedes Mat Instrumentat, 2 Blvd Ronceray,BP 3525, F-49035 Angers, France. TC 1 PD JUN 15 PY 2001 VL 389 IS 1-2 BP 187 EP 193 UT ISI:000169378200027 ER PT J AU Courant, B Hantzpergue, JJ Benayoun, S L'Huillier, JP TI Melting and solidification processes in a moving graphite-covered titanium surface subjected to multi-pulse laser irradiation SO JOURNAL OF PHYSICS D-APPLIED PHYSICS ID MODEL; CONVECTION; PREDICTION; ALLOYS; LAYER; DEPTH; BEAM AB Experimental results of surface melting by pulsed Nd-YAG laser irradiation of titanium covered with graphite powder were analysed in comparison with the results of numerical simulations. The simulations of the thermal events, such as the maximum of temperature gradient at the surface and the duration of the liquid state after irradiation, were found due to a semi-analytic model of the space-time temperature distributions which were induced by the irradiation treatments. These simulations were required in order to calculate a series of integrals by Simpson's numerical method. They have allowed us to explain the experimental results such as the incorporation of carbon in melted zone, which produces titanium carbide and possible graphite inclusions, as a function of the irradiation parameters. The perpetual absence in the thickness and the exceptional presence at the surface of the solidification structure resulting from the plane-front growth of titanium carbide have, moreover, been justified. C1 CRTT, Lab Applicat Mat Mecan, F-44602 St Nazaire, France. ENSAM, Lab Procedes Mat Instrumentat, F-49035 Angers, France. RP Courant, B, CRTT, Lab Applicat Mat Mecan, BP 406, F-44602 St Nazaire, France. TC 1 PD MAY 21 PY 2001 VL 34 IS 10 BP 1437 EP 1446 UT ISI:000169093500001 ER PT J AU Robert, P Peterson, AC Eyer, JL TI Neurofilament cytoskeleton disruption does not modify accumulation of trophic factor mRNA SO JOURNAL OF NEUROSCIENCE RESEARCH DE neurofilaments; microtubules; NFHLacZ transgenic mice; mRNA; neurotrophins ID NERVE GROWTH-FACTOR; CILIARY NEUROTROPHIC FACTOR; AMYOTROPHIC-LATERAL-SCLEROSIS; MESSENGER-RNA; AXONAL NEUROFILAMENTS; ALZHEIMERS-DISEASE; TRANSGENIC MICE; SPINAL-CORD; NF-H; EXPRESSION AB Previously we described a transgenic mouse model in which neurofilaments are sequestered in neuronal cell bodies and withheld from the axonal compartment. This model and other transgenic models with disrupted neurofilaments are used widely to investigate the role of the neurofilament cytoskeleton in normal neurons and in inherited or acquired diseases. To interpret such studies, it is important to establish whether the maldistribution of neurofilaments has major secondary consequences on the cell biology of the affected neurons. Notably, multiple perturbations of the nervous system simultaneously affect both the neuronal cytoskeleton and neurotrophin expression. To determine whether the expression of neurotrophic factors or their receptors is perturbed by a primary disruption in neurofilaments, we compared the accumulated mRNA levels for ciliary neuroptrophic factor (CNTF), nerve growth factor, neurotrophin 3, and the alpha CNTF receptor in mature transgenic mice and their littermate controls. Consistently with the prolonged survival of neurons expressing atypical or abnormally distributed neurofilaments, no obvious changes were observed for any of the mRNA species examined. (C) 2001 Wiley-Liss, Inc. C1 CHU Angers, INSERM,E9928, Lab Neurobiol & Transgenese, UPRES,EA3143, F-49033 Angers, France. McGill Univ, Royal Victoria Hosp, Mol Oncol Grp, Montreal, PQ, Canada. RP Eyer, JL, CHU Angers, INSERM,E9928, Lab Neurobiol & Transgenese, UPRES,EA3143, F-49033 Angers, France. TC 1 PD JUN 1 PY 2001 VL 64 IS 5 BP 487 EP 492 UT ISI:000169032700006 ER PT J AU Ohnuki, H Desbat, B Giffard, M Izumi, M Imakubo, T Mabon, G Delhaes, P TI The mechanism of charge transfer salt formation in the LB films of BEDO-TTF and fatty acid SO SYNTHETIC METALS DE infrared spectroscopy; metallic films; liquid/gas interfaces; Langmuir-Blodgett techniques AB The mixed valence state formation in a metallic Langmuir film on the water surface is reported. The mixing molecular system of BEDO-TTF (BO) and stearic acid (SA) forms a bilayer film, where a SA layer is lying on the BO layer, on the water surface. The polarization-modulated IR reflection absorption spectroscopy (PM-IRRAS) reveals a metallic optical property in the BO layer described by Drude model. Hydrogen bonding (R-COOHOOC-R)(-) anions in the SA layer are suggested by the comparison of IR spectrum of carboxylic salt of BEDT-TTF. C1 Ctr Rech Paul Pascal, F-33600 Pessac, France. Univ Bordeaux 1, Lab Phys Chim Mol, F-33405 Talence, France. Univ Angers, Lab Ingn Mol & Mat Organ, F-49045 Angers, France. Tokyo Univ Mercantile Marine, Appl Phys Lab, Koto Ku, Tokyo 1358533, Japan. RIKEN, Condensed Mol Mat Lab, Wako, Saitama 3510198, Japan. RP Ohnuki, H, Ctr Rech Paul Pascal, Ave A Schweitzer, F-33600 Pessac, France. TC 1 PD MAR 15 PY 2001 VL 120 IS 1-3 SI Sp. Iss. SI BP 889 EP 890 UT ISI:000168887500104 ER PT J AU Legrand, E TI Men with osteoporosis: are they being treated fairly? SO JOINT BONE SPINE ID BONE-MINERAL DENSITY; VERTEBRAL FRACTURES; ELDERLY MEN; AROMATASE DEFICIENCY; HIP-FRACTURES; RISK-FACTORS; OLDER MEN; WOMEN; PREVALENCE; ESTRADIOL C1 CHU Angers, Serv Rhumatol, F-49033 Angers, France. RP Legrand, E, CHU Angers, Serv Rhumatol, F-49033 Angers, France. TC 1 PD MAY PY 2001 VL 68 IS 3 BP 191 EP 193 UT ISI:000168946900001 ER PT J AU Alecu, C de Bray, JM Penisson-Besnier, I Pasco-Papon, A Dubas, F TI Trismus, pseudobulbar syndrome and cerebral deep venous thrombosis. SO REVUE NEUROLOGIQUE ID VEIN-THROMBOSIS; SINUS AB We report a case of cerebral deep venous thrombosis that manifested clinically by a pseudobulbar syndrome with major trismus, abnormal movements and static cerebellar syndrome. To our knowledge, only three other cases of deep cerebral venous thrombosis associated with cerebellar or pseudobulbar syndrome have been published since 1985. The relatively good prognosis in our patient could be explained by the partially intact internal cerebral veins as well as use of early anticoagulant therapy There was a spontaneous hyperdensity of the fair cerebri and the tentorium cerebelli on the brain CT scan, an aspect highly contributive to diagnosis. This hyperdensity of the fair cerebri was found in 19 out of 22 cases of deep venous thrombosis detailed in the literature. C1 CHU Angers, Serv Neurol A, F-49033 Angers 01, France. CHU Angers, Serv Radiol C, F-49033 Angers, France. RP Dubas, F, CHU Angers, Serv Neurol A, F-49033 Angers 01, France. TC 1 PD MAR PY 2001 VL 157 IS 3 BP 309 EP 314 UT ISI:000168432000008 ER PT J AU Leblond, H TI Direct derivation of a macroscopic NLS equation from the quantum theory SO JOURNAL OF PHYSICS A-MATHEMATICAL AND GENERAL ID ELECTROMAGNETIC-WAVES AB The use of the matrix density formalism, and of a multiscale expansion, allows the derivation of a macroscopic nonlinear evolution equation for a short light pulse (a nonlinear Schrodinger equation), directly from the microscopic quantum mechanics equations. We consider the simple case of a monochromatic plane wave, interacting with independent two-level atoms, to show that such a computation is possible. For their linear part, the results agree with that of the linear dispersion theory, but the obtained nonlinear coefficient differs appreciably from that derived from the computation of the the so-called nonlinear susceptibilities, except in one simple particular situation. C1 Univ Angers, Lab POMA, F-49045 Angers 01, France. RP Leblond, H, Univ Angers, Lab POMA, 2 Bd Lavoisier, F-49045 Angers 01, France. TC 1 PD APR 13 PY 2001 VL 34 IS 14 BP 3109 EP 3123 UT ISI:000168386400017 ER PT J AU Kityk, IV Sahraoui, B TI Infrared-induced nonlinear optical effects in chalcogenide glasses SO JOURNAL OF CHEMICAL PHYSICS ID INDUCED 2ND-HARMONIC GENERATION; PERIOD FIBER GRATINGS; 2-PHOTON ABSORPTION; LASER-PULSES; ORDER; POLYMERS AB The second-order nonlinear optical effects such as second harmonic generation (SHG) and linear electrooptics (LEO) in the middle infrared spectral range (5-15 mum) are experimentally observed for the first time. We have found that the novel As2Te3-BaBr2-BiCl2 chalcogenide glasses, possessing transmission windows within the 3-45 mum spectral range, have both photoinduced SHG equal to 0.0012 pm/V and comparable values of photoinduced electrooptics effect at wavelength of excitation (lambda =10.6 mum). The photoinduced anharmonic electron-phonon interaction play the major role in the observed phenomena. A good correlation between the IR-induced nonlinear optical susceptibilities and the photoinduced anharmonic electron-quasi-phonon interactions is demonstrated. The pump-probe experiment shows that the intensity-dependent maximum of the LEO is shifted towards the higher delaying times comparing with the SHG ones. Comparison of theoretical simulations with experimental measurements indicate dominant role of the electron-phonon anharmonic subsystem in the observed effects. (C) 2001 American Institute of Physics. C1 Inst Phys WSP, Czestochowa, Poland. Univ Angers, Fac Sci, Lab POMA, UMR CNRS, F-49045 Angers, France. RP Kityk, IV, Al Armii Krajowej 13-15, PL-42201 Czestochowa, Poland. TC 1 PD MAY 8 PY 2001 VL 114 IS 18 BP 8105 EP 8112 UT ISI:000168254000044 ER PT J AU Lascault, G Pansard, Y Scholl, JM Abraham, P Dupuis, JM Victor, J Copie, X Alonso, C Sarrazin, E TI Dual chamber rate responsive pacing and chronotropic insufficiency: Comparison of an activity sensor respiratory sensor and a double sensor during exercise and during normal everyday activity SO ARCHIVES DES MALADIES DU COEUR ET DES VAISSEAUX ID INITIAL CLINICAL-EXPERIENCE; QUALITY-OF-LIFE; CARDIAC-PACEMAKERS; LONG-TERM; 2 SENSORS AB Late responsive DDD pacemakers are the most technically advanced devices presently available. These pacemakers are particularly useful in patients with chronotropic insufficiency when the sinus node is incapable of accelerating during exercise. The latest pacemakers have two sensors to reproduce optimal physiological sinus acceleration. The aim of this study was to analyse the performances of a new rate responsive pacemaker with a double activity and respiratory sensor, the interaction of which is automatically controlled by a sophisticated algorithm, in 12 patients (8 men and 4 women) with a mean age of 75 +/- 7 years. Analysis was based on the performance of the sensors used singly or in association: -during three exercise stress tests with measurement of the VO2 max : - during everyday activities using the data archived by the pacemaker and the answers to a simplified questionnaire on quality of life. The results showed that during exercise stress testing with measurement of VO2 max, the best performances were obtained with the double sensor or the respiratory sensor compared with the activity sensor alone, suggesting that these two sensors are more effective in intense exercise. This tendency was also observed in the analysis of the memory bank of the pacemaker which showed that the total duration of the faster heart rates was greater with the two sensors. On the other hand, the quality of life was not significantly different, whichever sensor was studied. Longer scale trials are necessary to appreciate the real value of these new double sensor pacing devices and to identify the best indications for their usage. C1 Ctr Cardiol Nord, St Denis, Reunion. Hop Bichat, F-75877 Paris, France. CHU Angers, Angers, France. Medtron France, Boulogne, France. RP Lascault, G, Ctr Cardiol Nord, St Denis, Reunion. TC 1 PD MAR PY 2001 VL 94 IS 3 BP 190 EP 195 UT ISI:000168194700004 ER PT J AU Pagano, A Lode, T Crochet, PA TI New contact zone and assemblages among water frogs of Southern France SO JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH DE Rana radibunda; R. kl. esculenta; R. lessonae; R. perezi; R. kl. grafi; hybrid zone; hybridogenesis; LP tadpoles ID GERM-LINE EXCLUSION; INTROGRESSIVE HYBRIDIZATION; HYBRIDOGENETIC FROGS; RANA-RIDIBUNDA; EVOLUTION; HYBRIDS; ORIGIN; GENOME AB Water frogs are involved in several hybridogenetic complexes. However, investigations in Southern and Western France produced evidence of new hybridization events between R. lessonae and R. perezi (some new hybrids tadpoles were discovered), and the existence of new assemblages [R-G, L-P, R-P, P-R-G: (R, ridibunda; G, graft; L, lessonae; P, perezi)]. In respect to hybridogenesis, these assemblages offer opportunities for both primary and secondary hybridizations. Such peculiarities may be explained either by introductions or by relic populations and may influence water frog evolution. C1 Univ Angers, Lab Ecol Anim, F-49045 Angers, France. Stn Biol Tour Valat, F-13200 Arles, France. RP Pagano, A, Univ Angers, Lab Ecol Anim, Campus Belle Beille,2 Bvd Lavoisier, F-49045 Angers, France. TC 1 PD MAR PY 2001 VL 39 IS 1-2 BP 63 EP 67 UT ISI:000168090000006 ER PT J AU Di Martino, V Rosenheim, M Dorent, R Opolon, P Cadranel, JF Gandjbachkh, I Lunel, F TI Hepatitis B infection in heart transplant recipients - Reply SO GASTROENTEROLOGY ID VIRUS C1 CHU Pitie Salpetriere, Dept Hepatogastroenterol, F-75634 Paris, France. CHU Pitie Salpetriere, Dept Publ Hlth, F-75634 Paris, France. CHU Pitie Salpetriere, Dept Cardiovasc Surg, F-75634 Paris, France. CHU Angers, Dept Virol, F-49033 Angers, France. RP Di Martino, V, CHU Pitie Salpetriere, Dept Hepatogastroenterol, F-75634 Paris, France. TC 1 PD APR PY 2001 VL 120 IS 5 BP 1313 EP 1313 UT ISI:000167713700040 ER PT S AU Guerin, F Dumon, B Hambli, R TI Determining the shape parameter of a Weibull distribution from mechanical damage models SO ANNUAL RELIABILITY AND MAINTAINABILITY SYMPOSIUM, 2001 PROCEEDINGS SE PROCEEDINGS : ANNUAL RELIABILITY AND MAINTAINABILITY SYMPOSIUM DE Weibull distribution; log-normal distribution; mechanical damage; shape parameter; limited fatigue AB The aim of this paper is to show that it is possible to determine the shape factor of the weibull distribution relating to a mechanical damage model. In particular, we define the shape parameter linked to damage by limited fatigue. Also, the parameters of distribution of cycles number to failure is characterized using the limited fatigue approach by Basquin model. After, the Weibull parameters (beta and eta) are estimated by the method of moments. This work is currently used for other modes in order subsequently to obtain more precise conclusions when one analyses a Weibull sheet. C1 ISTIA, Qual & Reliabil Dept, F-49000 Angers, France. RP Guerin, F, ISTIA, Qual & Reliabil Dept, 62 Av Notre Dame Lac, F-49000 Angers, France. TC 1 PY 2001 BP 156 EP 160 UT ISI:000167729400027 ER PT J AU Boyer, J TI The place of ERCP and sphincterotomy in biliary acute pancreatitis SO GASTROENTEROLOGIE CLINIQUE ET BIOLOGIQUE DE biliary acute pancreatitis; gallstones; endoscopic sphincterotomy ID ACUTE GALLSTONE PANCREATITIS; ENDOSCOPIC SPHINCTEROTOMY; DUCT OBSTRUCTION; SURGERY; PAPILLOTOMY; MIGRATION; OPOSSUM; TRACT; CHOLECYSTECTOMY; STONES C1 CHU Angers, Serv Hepatogastroenterol, F-49033 Angers 01, France. RP Boyer, J, CHU Angers, Serv Hepatogastroenterol, F-49033 Angers 01, France. TC 1 PD JAN PY 2001 VL 25 IS 1 SU Suppl. S BP S122 EP S127 UT ISI:000167604600022 ER PT J AU Khassouani, CD Soulaymani, R Jana, M Mauras, Y Allain, P TI Blood mercury concentrations in the population of Rabat area, Morocco SO BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY ID FISH CONSUMPTION; METHYLMERCURY; EXPOSURE; AMALGAM; WOMEN; BASIN; LEAD C1 Poisoning Ctr Rabat, Rabat 11400, Morocco. CHU Angers, Lab Pharmacol & Toxicol, F-49033 Angers 01, France. Marrakech Univ Sci, Dept Biol, Marrakech, Morocco. RP Allain, P, CHU Angers, Lab Pharmacol & Toxicol, 4 Rue Larrey, F-49033 Angers 01, France. TC 1 PD APR PY 2001 VL 66 IS 4 BP 439 EP 442 UT ISI:000167518900005 ER PT J AU Darwich, AR TI About the absolute continuity and orthogonality for two probability measures SO STATISTICS & PROBABILITY LETTERS DE absolute continuity; orthogonality; Markov chain; product measures AB We give necessary and sufficient conditions for two probability measures to be absolute continuous or orthogonal. In many cases, these conditions can be easily checked and, in certain circumstances, it may be presented in terms of chi (2)-distances. This work extends results obtained in Dion and Ferland (J. Statist. Planning Inference 43 (1995) 235), Kabanov et al. (Math. USSR-Sb 33 (2) (1977) 203), and Skouras (J. Statist. Planning Inference 75 (1998) 1) (C) 2001 Elsevier Science B.V. All rights reserved. C1 Univ Angers, F-49045 Angers 01, France. TC 1 PD MAR 15 PY 2001 VL 52 IS 1 BP 1 EP 8 UT ISI:000167311000001 ER PT J AU Rouleau, F Baudusseau, O Dupuis, JM Victor, J Geslin, P TI Incidence and timing of thyroid dysfunction with long term amiodarone therapy. SO ARCHIVES DES MALADIES DU COEUR ET DES VAISSEAUX ID ANTITHYROID ANTIBODIES; DISEASE; HYPOTHYROIDISM AB The aim of this retrospective study was to assess the incidence, timing and classical presentations of thyroid dysfunction in patients treated long-term with amiodarone. Ninety patients (sex ratio M/W 67/33, mean age 71 years) were followed up for 48 months. The daily dose of amiodarone was 200 mg after loading doses during the hospital stay for atrial fibrillation. The average treatment period was 33 +/- 19 months. Hypothyroidism, observed in 5.5% of cases, occurred at variable intervals with few clinical manifestations. Hyperthyroidism was more common (12.2%), occurred increasingly with time, and was clinically symptomatic with recurrent supraventricular arrhythmias in 36% of cases. Long-term amiodarone therapy causes undesirable thyroid side-effects for nearly 1 out of 3 patients. Hyperthyroidism is more common than hypothyroidism and may be explained by increased iodine intake. The increasing incidence of hyperthyroidism with time suggests a cumulated dose relationship, a situation which is not observed with hypothyroidism. C1 CHU Angers, Serv Cardiol, F-49000 Angers, France. RP Rouleau, F, CHU Angers, Serv Cardiol, Rue Larrey, F-49000 Angers, France. TC 1 PD JAN PY 2001 VL 94 IS 1 BP 39 EP 43 UT ISI:000166966700006 ER PT J AU Hennekine, S Pessaux, P Regenet, N Fauvet, R Tuech, JJ Arnaud, JP TI Double appendicitis: a rare clinical presentation of appendix verriformes duplex SO PRESSE MEDICALE C1 CHU Angers, Serv Chirurg Viscerale, F-49033 Angers 01, France. RP Arnaud, JP, CHU Angers, Serv Chirurg Viscerale, F-49033 Angers 01, France. TC 1 PD JAN 13 PY 2001 VL 30 IS 1 BP 23 EP 24 UT ISI:000166660300006 ER PT J AU de Gentile, L TI Cercarian dermatitis, an emergent problem? SO MEDECINE ET MALADIES INFECTIEUSES C1 CHU Angers, Lab Parasitol Mycol, F-49033 Angers, France. RP de Gentile, L, CHU Angers, Lab Parasitol Mycol, 4 Rue Larrey, F-49033 Angers, France. TC 0 PD JUN PY 2001 VL 31 SU Suppl. 3 BP 356S EP 357S UT ISI:000181166600009 ER PT J AU Plaisant, O Chung, CS Uhl, JF Sourice, S Verdeille, S Martin-Bouyer, Y Gillot, C Mercier, P TI The origin of the azygos venous system, as deduced from an anatomical and radiological study employing a corrosion technique SO EUROPEAN JOURNAL OF MORPHOLOGY ID INJECTION; PLEXUS AB The aim of this study is not only to describe the origin of the human azygos venous system by performing a 3-D reconstruction of a CT scan but also to evaluate the value of the techniques employed in investigating the topographical anatomy of a venous system in the body. Following perfusion with saline to wash away the blood, we injected an ALTUFIX/MINIUM mixture into the azygos vein of a cadaver. The head and trunk were subsequently corroded with hydrochloric acid (HCl). A CT scan of the trunk was obtained both before and after corrosion. According to the spatial resolution of the CT scan, the thinnest identifiable detail was measurable as 0.5mm. The vertebral lumbar venous system was described, specifying the nomenclature of the lumbar veins (the lumbar veins being designated according to the vertebral body along which they run). On the right side, the lumbar veins at L2 and at L3 formed the lateral root of the azygos vein. On the left side, the vein at L2 formed the reno-azygo-lumbar arch (of Lejars). The lumbar veins, and the origin of the azygos system, were described and compared with previous studies. The 3-D reconstruction showed the importance of veins associated with the posterior paravertebral muscles. This description poses the problem of the metamerisation of the veins, but further evidence is required. Comparisons of the CT scans, 3-D reconstructions, and the ALTUFIX models of the veins obtained from the corrosion technique allowed verification of the 3-D reconstruction and correction of the errors inherent in a computer reconstruction. It is concluded that the description, and understanding, of such a complex system as the vertebral venous system is more valid when the results obtained using different techniques are compared. C1 Univ Paris 05, Inst Anat, F-75006 Paris, France. Lab Anat, Angers, France. Serv Radiol Clin Val dOr, St Cloud, France. Harvard Univ, Sch Med, Boston, MA USA. RP Plaisant, O, Univ Paris 05, Inst Anat, 45 Rue St Peres, F-75006 Paris, France. TC 0 PD OCT PY 2001 VL 39 IS 4 BP 193 EP 201 UT ISI:000179386100001 ER PT S AU Amghar, T Charnois, T Battistelli, D TI Aspecto-temporal data and lexical representations in French within Simple Conceptual Graphs on the basis of Semantico-Cognitive Schemes SO CONCEPTUAL STRUCTURES: BROADENING THE BASE, PROCEEDINGS SE LECTURE NOTES IN ARTIFICIAL INTELLIGENCE AB This paper deals with the modeling of time, aspect and verbal meanings in natural language processing within Simple Conceptual Graphs (SCG) by way of Semantico-Cognitive Schemes (SCS) and the aspecto-temporal theory. The expression of a semantico-cognitive representation within SCGs is automatically tractable. The SCS allows us to build a representation of a text taking into account the information about time and aspect. It allows us to represent fine subtleties of natural language. On the other hand, the Conceptual Graphs formalism provides a powerful inferential mechanism which makes it possible to reason from texts. Our work bears on Rench texts. A text is represented by two different structures both represented within the SCG model. The first structure models the semantico-cognitive representation while the second one is the temporal diagram representing the temporal constraints between the situations described in the text. Linking these structures leads us to slightly extend the original SCG model. C1 Univ Angers, LERIA, F-49045 Angers 01, France. Univ Paris 13, LIPN UPRES A 7030, F-93430 Villetaneuse, France. Univ Paris 04, Equipe La Lic, F-75006 Paris, France. RP Amghar, T, Univ Angers, LERIA, 2 Bd Lavoisier, F-49045 Angers 01, France. TC 0 PY 2001 VL 2120 BP 29 EP 43 UT ISI:000175011100003 ER PT S AU Dibie-Barthelemy, J Haemmerle, O Loiseau, S TI Refinement of conceptual graphs SO CONCEPTUAL STRUCTURES: BROADENING THE BASE, PROCEEDINGS SE LECTURE NOTES IN ARTIFICIAL INTELLIGENCE ID SYSTEMS AB The semantic validation of a knowledge base (KB) consists in checking its quality according to constraints given by an expert. The refinement of a KB consists in correcting the errors that are detected during the validation, in order to restore the KB validity. We propose to perform the semantic validation and refinement of a KB composed of conceptual graphs in two stages. First, we study the coherence of the KB with respect to negative constraints, which represent the knowledge that the KB must not contain. When the KB is not coherent, we propose a solution to correct all the errors of the KB. Second, we study the completeness of the KB with respect to positive constraints, which represent the knowledge that the KB must contain. When the KB is not complete, we propose an assistant, which helps the user to correct the errors of the KB one by one. C1 Inst Natl Agron Paris Grignon, F-75231 Paris 05, France. Univ Angers, UFR Sci, F-49045 Angers, France. RP Dibie-Barthelemy, J, Inst Natl Agron Paris Grignon, 16 Rue Claude Bernard, F-75231 Paris 05, France. TC 0 PY 2001 VL 2120 BP 216 EP 230 UT ISI:000175011100016 ER PT J AU Ritz, P Ryann, M Patureau-Mirand, P Legrand, P Couet, C Gueguen, L De Courcy, GP Guilland, JC Birlouez-Aragon, I Grolier, P Garabedian, M Azais-Braesco, V Sauvant, P Collet-Ribbing, C TI Nutritional Recommendations for the French Population SO SCIENCES DES ALIMENTS ID FATTY-ACID COMPOSITION; CORONARY-HEART-DISEASE; ALPHA-LINOLENIC ACID; INDISPENSABLE AMINO-ACIDS; ENDOGENOUS FECAL CALCIUM; PARATHYROID-HORMONE SECRETION; CARDIOVASCULAR RISK-FACTORS; SERUM CHOLESTERYL ESTERS; EPIDERMAL GROWTH-FACTOR; HIGH-CARBOHYDRATE DIETS C1 CHU Angers, Angers, France. INRA, Theix, France. INRA, Ensar, F-35042 Rennes, France. CHU Tours, Tours, France. INRA, Jouy En Josas, France. ISTNA, CNAM, Paris, France. Sch Med, Dijon, France. Ina PG, Paris, France. INRA, Clermont Ferrand, France. CNRS, Paris, France. Afssa, Paris, France. RP Ritz, P, CHU Angers, Angers, France. TC 0 PY 2001 VL 21 IS 4 BP 315 EP + UT ISI:000173517100001 ER PT J AU Ziani, M Tuech, JJ Chautard, D Regenet, N Pessaux, P Randriamananjo, S Arnaud, JP TI Palliative treatment of rectal carcinoma using a urologic resectoscope SO GASTROENTEROLOGIE CLINIQUE ET BIOLOGIQUE DE rectal carcinoma; palliative treatment; endoscopy; urologic resectoscope ID ENDOSCOPIC TRANSANAL RESECTION; METAL STENTS; TUMORS; CANCER; ADENOCARCINOMA; MANAGEMENT; SURGERY; LASER AB Objective - The aim of this study was to report our experience with endoscopic transanal resection (ETAR) using a urologic resectoscope for palliative treatment of rectal carcinoma. Methods - Outcome in patients who underwent ETAR with a urologic resectoscope between October 1992 and March 1999 are reported. Results - The 60 ETAR procedures were performed in 37 patients (19 men and 18 women, median age 82 years). Morbidity was 10% (6 patients) and mortality was 2.7% (1 patient). Median hospital stay for the procedure was 5 days. Symptom control was achieved in 86% of the patients (40% partial control, 46% complete control). Colostomy was performed in 8 patients 7 months after ETAR. At study end, 4 patients were alive. Median survival was 14 months (range 0 - 62), The 1-, 2-, and 5-year survival rates were 54, 32 and 5%, respectively. Conclusion - ETAR is a simple, minimally invasive and economical method for palliative treatment of patients with rectal carcinoma. ETAR is a useful addition to the surgeon's armamentarium. C1 CHU Angers, Dept Chirurg Digest, F-49033 Angers 01, France. RP Tuech, JJ, CHU Angers, Dept Chirurg Digest, 4 Rue Larrey, F-49033 Angers 01, France. TC 0 PD NOV PY 2001 VL 25 IS 11 BP 957 EP 961 UT ISI:000173537800003 ER PT J AU Dobo, I Mossuz, P Campos, L Girodon, F Allegraud, A Latger-Cannard, V Boiret, N Pineau, D Wunder, E Zandecki, M Praloran, V Hermouet, S TI Comparison of four serum-free, cytokine-free media for analysis of endogenous erythroid colony (EEC) growth in polycythemia vera (PV) and essential thrombocythemia (ET). SO BLOOD C1 CHU Angers, Hematol Lab, Angers, France. CHU Grenoble, Hematol Lab, F-38043 Grenoble, France. CHU St Etienne, Hematol Lab, St Etienne Du Rouvray, France. CHU Dijon, Hematol Lab, Dijon, France. CHU Limoges, Hematol Lab, Limoges, France. CHU Nancy, Hematol Lab, Nancy, France. CHU Clermont Ferrand, Hematol Lab, Clermont Ferrand, France. CHU Nantes, Hematol Lab, F-44035 Nantes 01, France. ETS Mulhouse, Hematol Lab, Mulhouse, France. TC 0 PD NOV 16 PY 2001 VL 98 IS 11 PN Part 2 BP 278B EP 278B UT ISI:000172134201263 ER PT J AU Dube, L Daenen, S Kouatchet, A Soltner, C Alquier, P TI Ectopic ACTH syndrome revealed by a severe metabolic alkalosis SO ANNALES FRANCAISES D ANESTHESIE ET DE REANIMATION DE ectopic ACTH syndrome; metabolic alkalosis; small cell lung carcinoma ID CUSHINGS-SYNDROME; DIAGNOSIS AB Metabolic alkalosis is frequently observed in critically ill patients. Etiologies are numerous but endocrinal causes are rare. We report a case of a patient with severe respiratory insufficiency, metabolic alkalosis and hypokalemia. The evolution was fatal. Further explorations revealed an ectopic Adrenocorticotropine Hormone syndrome. The initial tumor was probably a small cell lung carcinoma. (C) 2001 Editions scientifiques et medicales Elsevier SAS. C1 CHU Angers, Dept Anesthesie Reanimat, F-49035 Angers 01, France. RP Dube, L, CHU Angers, Dept Anesthesie Reanimat, 4 Rue Larrey, F-49035 Angers 01, France. TC 0 PD DEC PY 2001 VL 20 IS 10 BP 860 EP 864 UT ISI:000173365800007 ER PT J AU De Geyer, G Boucraut-Baralon, C TI Feline herpes virus type 1 and ocular diseases in the cat SO PRATIQUE MEDICALE ET CHIRURGICALE DE L ANIMAL DE COMPAGNIE DE feline herpes virus type 1; cat; conjunctivitis; keratitis; PCR ID POLYMERASE CHAIN-REACTION; CLINICALLY HEALTHY CATS; RESPIRATORY-TRACT DISEASE; VIRAL RHINOTRACHEITIS; CORNEAL SEQUESTRATION; HERPESVIRUS-1; CONJUNCTIVITIS; KERATITIS; INFECTION; REPLICATION AB The feline herpes virus type 1 (FHV-1), also know as the feline rhinotracheitis virus, is an infectious agent proper to cats. It is characterised not only by its cytolytic effect on certain epithelia, particulary on the conjonctiva and the cornea, but also by its possible recurrence. Ocular symptomes that tire secondary to FHV-1-1 may be typical, (conjunctivitis, superficial keratitis, stromal keratitis) or less typical (lacrymal deficiency eosinophil conjunctivitis, eosinophilic keratitis, corneal sequestra, uveitis). The presence of FHV-1-1 in the eye can be diagnosed within forty-eight hours using simple samples and the Polymerase Chain Reaction technique (PCR). Medical treatment does not enable the virus to be eliminated but it generally cares the current disorders. Surgery can be used to treat certain ocular complications related to FHV-1, such as symblepharon. C1 ENVT, Lab Scanelis, F-31076 Toulouse 03, France. RP De Geyer, G, 35 Ave Patton, F-49000 Angers, France. TC 0 PD SEP-OCT PY 2001 VL 36 IS 5 BP 461 EP 471 UT ISI:000172664800010 ER PT J AU Meslier, N Ouksel, H Racineux, JL TI Obstructive sleep apnea syndrome and cardiovascular risk. SO REVUE NEUROLOGIQUE ID CORONARY-ARTERY DISEASE; CARDIAC-ARRHYTHMIAS; CONDUCTION DISTURBANCES; ESSENTIAL-HYPERTENSION; ASSOCIATION; MEN; PREVALENCE; MORTALITY; POPULATION; PRESSURE AB Numerous studies have shown that obstructive sleep apnea syndrome (OSAS) is associated with an increased cardiovascular morbidity and mortality, Obstructive sleep apnea syndrome and cardiovascular disorders are frequent diseases. They share several risk factors such as age, gender, obesity, smoking, and alcohol. Therefore it is difficult to demonstrate that OSAS is a cardiovascular risk factor, independent of previously known factors. Recent epidemiological studies and trials, consistent with the results of previous studies, have demonstrated a strong association between OSAS and systemic hypertension. They also suggest that there is a possible cause-effect relation between OSAS and coronary artery disease or cardiac arrhythmias. Studies demonstrating that early recognition and treatment of OSAS may be effective in reducing these cardiovascular complications are still needed. C1 CHU Angers, Serv Pneumol, Unite Pathol Sommeil, F-49033 Angers, France. RP Meslier, N, CHU Angers, Serv Pneumol, Unite Pathol Sommeil, F-49033 Angers, France. TC 0 PD NOV PY 2001 VL 157 IS 11 PN Part 2 BP S42 EP S45 UT ISI:000172849600009 ER PT J AU Pessaux, P Tuech, JJ Hennekinne, S Regenet, N Arnaud, JP TI The use of occluder pin in severe presacral hemorrhage SO ANNALES DE CHIRURGIE DE occluder pin; presacral hemorrhage C1 CHU Angers, Serv Chirurg Viscerale, F-49033 Angers 01, France. RP Pessaux, P, CHU Angers, Serv Chirurg Viscerale, 4 Rue Larrey, F-49033 Angers 01, France. TC 0 PD DEC PY 2001 VL 126 IS 10 BP 1019 EP 1019 UT ISI:000172883300014 ER PT J AU Dayot, F Bataillard, A Kere, C Duces, F Bachmann, P Blanc-Vincent, MP Besnard, B Bonneteau, C Champetier, S Claude, M Combret, D Cometto, F Duguet, A Duval, N Finck, C Freby-Lehner, A Garabige, V Lallemand, Y Massoud, C Meuric, J Montane, C Poiree, B Puel, S Rossignol, G Roux-Bournay, P Simon, M Tran, M TI Standards, options and recommendations: Good clinical practice in the dietetic management of cancer patients: hospital catering SO BULLETIN DU CANCER DE hospital catering; dietetics; nutrition; guidelines; neoplasms; adult ID NUTRITIONAL MANAGEMENT; NECK-CANCER; HEAD AB Context. The "Standards, Options and Recommendations" (SOR) project, started in 1993, involves a collaboration between the Federation of the French Cancer Centres (FNCLCC), the 20 French Regional Cancer Centres, some French public university and general hospitals and private Clinics and medical scientific societies. Its main objective is the development of clinical practice guidelines to improve the quality of health care and outcome for cancer patients. The methodology is based on a literature review followed by a critical appraisal by a multidisciplinary group of experts to produce the draft guidelines which are then validated by specialists in cancer care delivery. Objectives. To develop clinical practice guidelines for hospital catering for cancer patient using the methodology developed by the Standards, Options and Recommendations project. Methods. Data were identified by a literature search of Medline (R) and the reference lists of experts in the groups. After the guidelines were drafted, they were validated by independent reviewers. Results. The main recommendations are: 1) While taking into consideration the specific needs of cancer patients, the dietician is responsible for the hygiene, the sanitary quality of alimentation, the equilibrium and nutritional quality of the hospital catering. 2) Ordering and distribution of meals, and clearing up afterwards contribute to the quality of hospital catering and the personnel who do this should have tune and be willing to listen to the patients. 3) The ordering of meals should be adapted to individual patient's requirements and must take into account the patient's medication. 4) The method of transporting the food chosen by the institution (cold or warm method) should be respected. The personnel responsible should receive regular and specific training to use the method correctly. 5) The intake of patients with nutritional followup should be reliably and reproducibly evaluated by the personnel after every meal. 6) Patient satisfaction should be assessed once a year and the results of this assessment used to improve the quality of hospital catering. 7) The dietician is the interface between the accounts department, the medical wards, the hospital catering department and the patients. C1 FNCLCC, Stand Opt Recommandat, F-75654 Paris 13, France. Ctr Rene Gauducheau, F-44035 Nantes, France. Ctr Val Aurelle, Montpellier, France. Ctr Paoli Calmette, Marseille, France. Ctr Leon Berard, F-69373 Lyon, France. Ctr Paul Papin, Angers, France. Inst Bergonie, Bordeaux, France. Inst Jean Godinoi, F-51056 Reims, France. Hosp Neurol Cardiol, Lyon, France. Ctr Jean Perrin, Clermont Ferrand, France. Ctr Oscar Lambret, F-59020 Lille, France. Ctr Francois Baclesse, F-14021 Caen, France. Ctr Paul Strauss, Strasbourg, France. Ctr Rene Huguenin, St Cloud, France. Inst Curie, Paris, France. Inst Claudius Regaud, Toulouse, France. Inst Gustave Roussy, Villejuif, France. Ctr Alexis Vautrin, Nancy, France. RP Dayot, F, FNCLCC, Stand Opt Recommandat, 101 Rue Tolbiac, F-75654 Paris 13, France. TC 0 PD OCT PY 2001 VL 88 IS 10 BP 1007 EP 1018 UT ISI:000172210600009 ER PT J AU Guichet, C Vuillemin, E Pilette, C Ternisien, C Cales, P TI Anemia: a new cause of portal vein thrombosis? SO GASTROENTEROLOGIE CLINIQUE ET BIOLOGIQUE C1 Ctr Hosp Univ, Serv Hepatogastroenterol, Angers, France. Ctr Hosp Univ, Hematol Lab, Angers, France. RP Guichet, C, Ctr Hosp Univ, Serv Hepatogastroenterol, Angers, France. TC 0 PD AUG-SEP PY 2001 VL 25 IS 8-9 BP 833 EP 834 UT ISI:000172332700021 ER PT J AU Bagwell, CB Clark, GM Spyratos, F Chassevent, A Bendahl, PO Stal, O Killander, D Jourdan, ML Romain, S Hunsberger, CB Wright, S Baldetorp, B TI Multivariate analyses of flow cytometric S-phase and ploidy as node-negative breast cancer prognostic factors: an international and multi-center study. SO BREAST CANCER RESEARCH AND TREATMENT C1 Ver Software House Inc, Software Dev, Topsham, ME USA. Baylor Coll Med, Breast Ctr, Houston, TX 77030 USA. Ctr Rene Huguenin, St Cloud, France. Ctr Paul Papin, Angers, France. Jubileum Inst, Dept Oncol, Lund, Sweden. Linkoping Univ, Fac Hlth Sci, Dept Oncol, Linkoping, Sweden. Hop Bretonneau, Tours, France. Assistance Publ Hop, Marseille, France. Esoterix Oncol, Brentwood, TN USA. TC 0 PY 2001 VL 69 IS 3 BP 260 EP 260 UT ISI:000172344800207 ER PT J AU Sylla, M Rivoire, G TI Degenerate two-beam coupling polarization properties in nonlinear materials SO JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS ID ABSORPTION; WAVE AB The nonlinear transmission change of a weak beam in the presence of an intense beam is measured and analyzed in various absorbing and scattering materials. Simultaneous contributions of different nonlinear processes are identified. Addition of the nonlinear refractive-index change and Rayleigh-wing scattering contributions yields particularly large transmission changes. (C) 2001 Optical Society of America. C1 Univ Angers, CNRS, UMR 6136, Lab Proprietes Opt Mat & Applicat, F-49045 Angers, France. RP Sylla, M, Univ Angers, CNRS, UMR 6136, Lab Proprietes Opt Mat & Applicat, 2 Bd Lavoisier, F-49045 Angers, France. TC 0 PD NOV PY 2001 VL 18 IS 11 BP 1612 EP 1619 UT ISI:000172089800009 ER PT J AU Buldyreva, J Rachet, F Chrysos, M TI Nitrogen isotropic Raman Q-branch profile in an extended frequency range SO PHYSICAL REVIEW A ID ROTATIONALLY INELASTIC RATES; INDUCED LIGHT-SCATTERING; THEORETICAL-ANALYSIS; ENERGY AB The room-temperature nitrogen fundamental isotropic Q-branch profile was both experimentally and theoretically studied in a large frequency interval of about 100 cm(-1) never explored before, and for densities ranging from 1 to 150 amagat. As regards experiment, this frequency interval could be probed thanks to a highly sensitive spontaneous Raman technique previously developed in our institute. As regards theory, such a large interval of frequencies made it possible to rigorously check. for the scalar relaxation case, the validity of the recently proposed and successfully applied to nitrogen anisotropic spectra energy-corrected sudden (ECS) formalism [J.V. Buldyreva and L. Bonamy, Phys. Rev. A 60, 370 (1999)]. For all the densities probed, an excellent agreement between calculated and measured profiles was attested within a frequency interval of 20 cm(-1) where the intensity decreases by more than three decimal orders from its peak value. The adequacy of the theory to experiment in such a wide frequency range confirmed the reliability of the used ECS modeling, especially for optical diagnostics purposes. C1 Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, UMR 6136, F-49045 Angers, France. RP Buldyreva, J, Univ Angers, Lab Proprietes Opt Mat & Applicat, CNRS, UMR 6136, 2 Blvd Lavoisier, F-49045 Angers, France. EM jeanna.buldyreva@univ-angers.fr TC 0 PD NOV PY 2001 VL 6405 IS 5 AR 052708 DI ARTN 052708 UT ISI:000172074200048 ER PT J AU Gardais, J Genevieve, F Foussard, G Delisle, V Zandecki, M TI Is there any significance for intracellular crystals in plasma cells from patients with monoclonal gammopathies? SO EUROPEAN JOURNAL OF HAEMATOLOGY DE multiple myeloma; monoclonal gammopathies; plasma cells; intracellular crystals; morphology ID MULTIPLE-MYELOMA; LIGHT-CHAIN; STORING HISTIOCYTOSIS; FANCONIS-SYNDROME; INCLUSIONS; MORPHOLOGY AB Several plasma cells morphological changes have been described in monoclonal gammopathies, including intracytoplasmic crystals. We report one case of indolent kappa -chain multiple myeloma with renal insufficiency, featuring plasma cells with Auer-rod-like intracytoplasmic inclusions. The relationship between such aberrations and those found in multiple-myeloma-associated adult Fanconi syndrome is discussed. The significance of intracellular storage and crystallisation of immunoglobulin within plasma cells remains partially unknown. C1 CHU Angers, Hematol Lab, F-49033 Angers 01, France. CHU Angers, Serv Med & Hematol D, F-49033 Angers, France. RP Gardais, J, CHU Angers, Hematol Lab, 4 Rue Larrey, F-49033 Angers 01, France. TC 0 PD AUG PY 2001 VL 67 IS 2 BP 119 EP 122 UT ISI:000171975900009 ER PT J AU Rouge-Maillart, C Pessaux, P Jousset, N Hubert, N Gosset, D Penneau, M TI Rights, responsibility, and results SO PRESSE MEDICALE AB Several important decisions were made in 2000 concerning the proof of malpractice and the fundamental principles of medical responsibility. in order to guarantee indemnities for victims of medical accidents, the French courts have facilitated the implication of medical responsibility for medical accidents. The notion of a "virtual fault" was developed allowing the courts to retain the responsibility of the surgeon for instance for injury to the sublingual nerve during extraction of a wisdom tooth or for injury to the popliteal artery (March 23, 2000). These decisions not only facilitate the demonstration of malpractice but also modify the definition of responsibility, all physicians being required to use all available means. Likewise, although jurisprudence asserts that a safe result is mandatory in certain areas, the essential obligation is the absence of "fault" and not the result despite the disquieting arguments put forward by the Paris appeals court in its January 15, 1999 decree. The patient's right to a result was sustained only in well defined areas. (C) 2001, Masson, Paris. C1 CHU Angers, Serv Urgences, F-49100 Angers, France. Inst Med Legale, Lille, France. RP Rouge-Maillart, C, CHU Angers, Serv Urgences, 4 Rue Larrey, F-49100 Angers, France. TC 0 PD OCT 6 PY 2001 VL 30 IS 28 BP 1380 EP 1383 UT ISI:000171746900001 ER PT J AU Changeux, B Lebrun, JL TI Microstructural modifications and material response in machining of steels SO JOURNAL DE PHYSIQUE IV ID SHEAR LOCALIZATION; CHIP FORMATION; MECHANICS AB In machining with a cutting tool (turning, milling, drilling....), the loading conditions for the cut material in the cutting zone are extremely severe, in terms of strain (some hundred %), strain rates (similar to 10(5) s(-1)), temperatures (some hundred degreesC) and temperature rates (similar to 10(6) Ks(-1)). The subsequent microstructural changes are analysed and the problem of the evaluation of the behaviour of the cut material (i.e. of the chip and the workpiece) is discussed, irk the view of building a predictive cutting model. X-ray diffraction, microscopy, as well as analytical modelling are used to investigate the small zone under solicitation ahead of the tool tip. Texture, hardness, phase transformation data inside the cutting products attest a very strong heterogeneity in the thermo-mechanical loading from places of the cutting zone to others, which makes cutting a problem difficult to treat under a unique behaviour view. C1 ENSAM Paris, F-75013 Paris, France. ENSAM Angers, LPMI, F-49035 Angers, France. RP Changeux, B, ENSAM Paris, 151 Blvd Hop, F-75013 Paris, France. TC 0 PD SEP PY 2001 VL 11 IS PR4 BP 213 EP 220 UT ISI:000171936600028 ER PT J AU Rochetaing, A Barbe, C Kreher, P TI Acute ischemic preconditioning and high subchronic co exposure independently increase myocardial tolerance to ischemia SO INHALATION TOXICOLOGY ID PROTEIN-KINASE-C; K-ATP CHANNELS; RAT HEARTS; GLOBAL-ISCHEMIA; VENTRICULAR-FIBRILLATION; REPERFUSION ARRHYTHMIAS; CORONARY FLOW; SMOOTH-MUSCLE; CONTRACTURE; PROTECTION AB This study was designed to investigate whether exposure to carbon monoxide (CO) could alter or raise the ischemic tolerance induced by preconditioning. To this end, isolated rat hearts were aerobically perfused for 20 min. Hearts were then randomized to two groups: (1) a further 20-min aerobic perfusion, and (2) ischemic preconditioning (2 cycles of 5 min of ischemia followed by 5 min of reperfusion). Hearts were then subjected to 25 min of low-flow (0.3 ml/min.) global ischemia (37 degreesC) and 30 min of reperfusion. In parallel studies, the same protocols were performed in hearts from rats previously exposed to subchronic CO (600 ppm for 2 wk). Ischemic preconditioning accelerated the development of ischemic contracture (onset = 6.0 +/- 0.3 vs. 8.6 +/- 0.9 min), increased the preischemic coronary flow (19.0 +/- 1.0 vs. 11.6 +/- 0.6 ml/min/g), improved contractile recovery (73.7 +/- 8.9 vs. 30.8 +/- 7.5%), but was without effect on reactive hyperemia (151.2 +/- 4.7 vs. 149.2 +/- 5.1%) and incidence of ventricular arrhythmia during reperfusion (55.6 vs. 60.0%) compared to a control group. CO exposure alone increased the baseline coronary flow (20.1 +/- 1.5 vs. 12.8 +/- 0.6 ml/min/g) and the contracture magnitude (54.8 +/- 6.8 vs. 37.1 +/- 4.8%), improved both contractile recovery (66.1 +/- 6.3 vs. 30.8 +/- 7.5%) and ventricular arrhythmia incidence (22.2 vs. 60.0%), and increased the hyperemic coronary flow (26.7 +/- 1.5 vs. 19.1 +/- 0.7%). Preconditioning after CO exposure exacerbated ischemic contracture ( shorter onset and higher magnitude), and increased the reactive hyperemia (29.8 +/- 1.4%), but raised the beneficial effects on contractile recovery (85.4 +/- 8.4%) without alteration of ventricular tachycardia prevention (22.2%). Thus, CO-exposed hearts could be preconditioned in the same way as normal myocardium. C1 UFR Sci, Lab Precondit & Remodelage Myocarde, F-49045 Angers, France. RP Rochetaing, A, UFR Sci, Lab Precondit & Remodelage Myocarde, 2 Blvd Lavoisier, F-49045 Angers, France. TC 0 PD NOV PY 2001 VL 13 IS 11 BP 1015 EP 1032 UT ISI:000171971400004 ER PT J AU Picquet, J Jousset, Y Papon, X Villapadiema, R Brillu, C Enon, B TI Surgery of the proximal vertebral artery. Indications and results SO JOURNAL DES MALADIES VASCULAIRES DE vertebral artery; surgery; vertebro-basilary insufficiency ID PERCUTANEOUS TRANSLUMINAL ANGIOPLASTY; POSTERIOR CIRCULATION; DISEASE; RECONSTRUCTION; STENOSIS AB Objectives : The purpose of this study was to: 1) detail the clinical presentations of diseases requiring revascularization of the vertebral artery and recall the guidelines of the 1975 Ad Hoc committee; 2) identify the explorations needed to detect vertebro-basilary insufficiency; 3) define operative indications since no consensus has been reached. Patients and methods : We reviewed retrospectively the files of 34 patients who underwent revascularization of the vertebral artery between January 1990 and December 2000. Results : Surgery of the vertebral artery accounted for 4.6% of our vascular surgery cases involving the brain. Fifty percent of the cases of vertebro-basilary insufficiency were caused by hemodynamic disorders and 26.5% by embolism; 23.5% of the patients had no neurological sign. The most frequent neurological signs were vertigo (44%), balance disorders (41%), long pathway involvement (32%). Vertebro-basilary reimplantation was performed in 76% of the cases. There were no cases of stroke and no deaths during the early postoperative period. There was one case of asymptomatic thrombosis. Mean follow-up was 33.6 months with no patients lost to follow-up. Overall survival was 85.29% at 3 years with patent arteries in 97.06% of the cases at 3 years. Conclusion : Our series is in agreement with others reported in the literature, emphasizing the good outcome achieved after vertebral artery surgery. Surgery helps improve signs of vertebro-basilary insufficiency with little operative risk. This surgery is rarely indicated but must be recognized. One must also resist the temptation to "treat images". C1 CHU Angers, Serv Chirurg Cardiovasc & Thorac, F-49033 Angers 01, France. RP Picquet, J, CHU Angers, Serv Chirurg Cardiovasc & Thorac, 4 Rue Larrey, F-49033 Angers 01, France. TC 0 PD OCT PY 2001 VL 26 IS 4 BP 237 EP 242 UT ISI:000171835600004 ER PT J AU Le Gall, S Francois, S Urier, N Genevieve, F d'Hermies, F Rachieru, P Ifrah, N TI Inaugural subretinal localization of recurrent acute myeoloblastic leucemia-5 SO PRESSE MEDICALE ID LEUKEMIA; MANIFESTATIONS; INFILTRATION AB Background Reduced visual acuity in patients with acute leucemia can result from many causes including an ocular localization. Case report A patient previously treated for acute myeloblastic leucemia-5 (AML5) developed bilateral vision impairment related to a subretinal localization of the leucemia. Meningeal and bone marrow relapse followed. The subretinal localization responded only to massive systemic steroid treatment. Discussion Although asymptomatic, ocular localizations are frequent in leucemia. Their prognostic impact depends on the ocular structure involved and on the chronology of onset-early or late in the leucemia course. The underlying pathophysiological mechanism of ocular involvement remains unexplained but hyperleucocytosis at presentation may be a risk factor and would justify at least systematic specialized examinations and discussion of prophylactic treatment (C) 2001, Masson, Paris. C1 CHU Angers, Hematol Serv, F-49033 Angers 1, France. CHU Angers, Serv Ophtalmol, F-49033 Angers, France. CHU Angers, Hematol Lab, F-49033 Angers 1, France. RP Ifrah, N, CHU Angers, Serv Med Malad Sang D, F-49033 Angers 1, France. TC 0 PD OCT 13 PY 2001 VL 30 IS 29 BP 1450 EP 1452 UT ISI:000171676700005 ER PT J AU Nalos, M Asfar, P Pittner, A Yang, Y Vassilev, D Georgieff, M Radermacher, P Froba, G TI Hemoxygenase (HO-1) inhibition is detrimental in hyperdynamic porcine endotoxaemia SO INTENSIVE CARE MEDICINE C1 Univ Klin Ulm, Abt Anasthesie, Ulm, Germany. CHU Angers, Angers, France. TC 0 PD SEP PY 2001 VL 27 SU Suppl. 2 BP S146 EP S146 UT ISI:000171249400045 ER PT J AU Asfar, P Nalos, M Pittner, A Theisen, M Ichai, C Ploner, F Georgieff, M Ince, C Bruckner, UB Leverve, XM Radermacher, P Froba, G TI ATP-MgCl2 effects on hepato-splanchnic haemodynamics and metabolism during hyperdynamic porcine endotoxaemia SO INTENSIVE CARE MEDICINE C1 CHU Angers, Angers, France. UJF, Lab Bioenerget Fond & Appl, Grenoble, France. CHU Nice, Nice, France. Univ Klin Ulm, Abt Anasthesie, Ulm, Germany. Univ Klin Ulm, Sekt Chir Forsch, Ulm, Germany. Univ Amsterdam, Acad Med Ctr, NL-1105 AZ Amsterdam, Netherlands. TC 0 PD SEP PY 2001 VL 27 SU Suppl. 2 BP S182 EP S182 UT ISI:000171249400184 ER PT J AU Beydon, L Uttman, L Jonson, B TI Dead space and respiratory mechanics to assess PEEP responders in patients ventilated for acute lung injury SO INTENSIVE CARE MEDICINE C1 CHU Angers, Dept Anesthesie, Angers, France. Univ Lund Hosp, Dept Clin Physiol, S-22185 Lund, Sweden. TC 0 PD SEP PY 2001 VL 27 SU Suppl. 2 BP S232 EP S232 UT ISI:000171249400378 ER PT J AU Pittner, A Nalos, M Asfar, P Yang, Y Ince, C Georgieff, M Radermacher, P Froba, G TI Selective iNOS inhibition corrects ileal mucosal acidosis without influencing villous microcirculation SO INTENSIVE CARE MEDICINE C1 Univ Ulm, Ulm, Germany. CHU Angers, Angers, France. Univ Amsterdam, Acad Med Ctr, NL-1105 AZ Amsterdam, Netherlands. TC 0 PD SEP PY 2001 VL 27 SU Suppl. 2 BP S234 EP S234 UT ISI:000171249400387 ER PT J AU Ter Minassian, A Desfontis, JC Gogny, M Beydon, L TI Cerebral of PO2+ pH and PCO2 at different levels of intracranial pressure: An animal study SO INTENSIVE CARE MEDICINE C1 CHU Angers, Dept Anesthesia, Angers, France. Sch Vet, Dept Physiol & Pharmacol, Nantes, France. TC 0 PD SEP PY 2001 VL 27 SU Suppl. 2 BP S260 EP S260 UT ISI:000171249400485 ER PT J AU Rouge-Maillart, C Pessaux, P Gosset, D Penneau, M TI Physician's civil responsibility: what's new in 2000? SO ANNALES DE CHIRURGIE DE information; fault; responsibility; obligation to achieve a result AB In France, during the last year, important jurisprudence was established by the French Supreme Court of Appeal concerning the physician's civil responsibility. On October 7, 1998, the Court decided that "the physician is not exempted for the obligation to provide information by the simple fact that these risks only materialize exceptionally". This means that from now, the physician must inform the patient of all risks that might influence the patient's decision, particularly information concerning life-threatening or severe consequences, but also, as in the past, concerning frequent even benign consequences. The limits of this jurisprudence and the completeness of the information, as established in 1998, are emergency, patient refusal and impossibility to inform the patient. In the decree of May 23, 2000, the Court gave its definition of impossibility to inform the patient, thus establishing the "therapeutic limits". But the judges recognized that the requirement for information delivery is independant of the necessary or unnecessary nature of the therapeutic act. However, in the decree of June 20, 2000, the Court established the conditions for awarding indemnities for defective information delivery. Defective information delivery is not sufficient in itself to constitute a civil offense. Real damage is also necessary. To be awarded with an indemnity, the patient must prove that the lack of information affected his/her decision to consent. If it appears that even if he/she had been well informed, the patient had consented to the care given, the physician would not be obliged to provide the patient with an indemnity. The judges want to find a compensation and make the proof easier for the patient. They accept the potential fault when an organ was injured in the course of an operation. But, these decisions concern the proof and they don't modify the medical responsibility. The physicians have got a duty to use reasonable skill and care and they don't have any obligation to achieve a certain result. Sometimes, the physician has got an obligation to achieve a certain result but it isn't a general rule (November 08, 2000). (C) 2001 Editions scientifiques et medicales Elsevier SAS. C1 CHU Angers, Consultat Med Legale, F-49100 Angers, France. Inst Medicolegal, F-59000 Lille, France. RP Rouge-Maillart, C, CHU Angers, Consultat Med Legale, 4 Rue Larrey, F-49100 Angers, France. TC 0 PD OCT PY 2001 VL 126 IS 8 BP 794 EP 800 UT ISI:000171617300013 ER PT J AU Payan, C Cottin, J Ramon, C Brault, C Lemarie, C TI Inactivation of the cultured hepatitis B virus with generally used household disinfectants SO ANNALES DE BIOLOGIE CLINIQUE C1 CHU Angers, Lab Bacteriol Virol Hyg, Angers, France. RP Payan, C, CHU Angers, Lab Bacteriol Virol Hyg, Angers, France. TC 0 PD OCT PY 2001 VL 59 SI Sp. Iss. SI BP 25 EP 26 UT ISI:000171485300008 ER PT J AU Roulot, D Degott, C Chazouilleres, O Castera, L Oberti, F Carbonell, N Benferhat, S Cales, P Valla, D TI Hepatic involvement in Turner syndrome: Idiopathic and heterogeneous lesions suggesting a role for vascular origin. SO HEPATOLOGY C1 Hop Avicenne, F-93009 Bobigny, France. Hop Beaujon, Beaujon, France. Hop St Antoine, F-75571 Paris, France. Jean Verdier Hosp, Bondy, France. Angers Hosp, Angers, France. TC 0 PD OCT PY 2001 VL 34 IS 4 PN Part 2 Suppl. S BP 507A EP 507A UT ISI:000171224701330 ER PT J AU Cales, P Vuillemin, E Chappard, D Cales, P TI Fractal dimension can distinguish models and pharmacological changes in liver fibrosis. SO HEPATOLOGY C1 Fac Med, Angers, France. TC 0 PD OCT PY 2001 VL 34 IS 4 PN Part 2 Suppl. S BP 547A EP 547A UT ISI:000171224701493 ER PT J AU Moal, F Aube, C Roux, J Croquet, V Oberti, F Torrisani, J Buscail, L Cales, P TI Treatment of hepatocellular carcinoma by octreotide in rat. SO HEPATOLOGY C1 Fac Med, Angers, France. INSERM, U531, Toulouse, France. TC 0 PD OCT PY 2001 VL 34 IS 4 PN Part 2 Suppl. S BP 675A EP 675A UT ISI:000171224702002 ER PT J AU Oberti, F Wang, J Moal, F Croquet, V Vuillemin, E Roux, J Gallois, Y Cales, P TI Hemodynamic and antifibrotic effects of the early and chronic administration of propranolol in two models of intrahepatic portal hypertensive rats. SO HEPATOLOGY C1 Fac Med, Angers, France. TC 0 PD OCT PY 2001 VL 34 IS 4 PN Part 2 Suppl. S BP 693A EP 693A UT ISI:000171224702075 ER PT J AU Baufreton, C Binuani, P Etcharry-Bouyx, F de Brux, JL TI Long-term neuropsychological outcome after retrograde cerebral perfusion SO EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY DE aorta; thoracic surgery; cardiopulmonary bypass; perfusion methods; brain/blood supply; neuropsychological tests; cognition disorders C1 Univ Hosp, Dept Cardiovasc Surg, F-49033 Angers 01, France. Univ Hosp, Dept Neurol, F-49033 Angers, France. RP Baufreton, C, Univ Hosp, Dept Cardiovasc Surg, 4 Rue Larrey, F-49033 Angers 01, France. TC 0 PD OCT PY 2001 VL 20 IS 4 BP 891 EP 892 UT ISI:000171527900056 ER PT J AU Sahraoui, B Derkowska, B Kryza, A Kityk, IV TI The IR photoinduced changes in the Y-Ba-Cu-O thin films SO OPTICA APPLICATA ID OXYGEN-DEFICIENT YBA2CU3OX; PERSISTENT PHOTOCONDUCTIVITY; RELAXATION AB The Y-Ba-Cu-O thin films are promising materials because they have not only superconducting properties but also good optical properties, particularly in the IR spectral range. We investigate the photoinduced optical changes in the IR region for oriented Y-Ba-Cu-O thin films deposited on <110> surface of ZnSe single crystals, using differential IR spectroscopy Fourier technique in the spectral region between 100 cm(-1) and 450 cm(-1). We have also found the photoinduccd photodarkening effect under the influence of the CO2 Pulse laser (lambda = 10.6 mum). The possibility of using the above-mentioned materials as promising materials for IR optoelectronics and nonlinear optics is discussed. C1 Univ Angers, Lab POMA, UFR Sci, UMR CNRS, F-49045 Angers, France. Inst Phys WSP, PL-42201 Czestochowa, Poland. RP Sahraoui, B, Univ Angers, Lab POMA, UFR Sci, UMR CNRS, 2 Bd Lavoisier, F-49045 Angers, France. TC 0 PY 2001 VL 31 IS 2 BP 445 EP 451 UT ISI:000171414900015 ER PT J AU Penisson-Besnier, I Dumez, C Saint-Cast, Y Jeanpierre, M Dubas, F TI Autosomal dominant scapulofacial myopathy not linked to 4q SO NEUROMUSCULAR DISORDERS DE facioscapulohumeral muscular dystrophy; 4q35 rearrangements; autosomal dominant muscular disorder C1 CHU Angers, Dept Neurol, Angers, France. Hop Cochin, Biochim Genet Lab, F-75674 Paris, France. TC 0 PD SEP PY 2001 VL 11 IS 6-7 BP 620 EP 620 UT ISI:000170822100033 ER PT J AU Delepine, S Furber, A Balzer, P Prunier, F Lejeune, JJ Geslin, P Beygui, F TI Distribution of the left ventricular systolic wall stress in patients with reperfused myocardial infarction SO EUROPEAN HEART JOURNAL C1 CHU Angers, Dept Cardiol, Angers, France. CHU Angers, Angers, France. TC 0 PD SEP PY 2001 VL 22 SU Suppl. S BP 179 EP 179 UT ISI:000170988300691 ER PT J AU Benard, T Pezard, P Baufreton, C Furber, A Merheb, M Lhoste, P Geslin, P TI Usefulness of stress TI-201 SPECT in the follow-up of asymptomatic patients after a coronary angioplasty SO EUROPEAN HEART JOURNAL C1 CHU Angers, Dept Cardiol, Angers, France. TC 0 PD SEP PY 2001 VL 22 SU Suppl. S BP 234 EP 234 UT ISI:000170988300890 ER PT J AU Dupouy, P Gilard, M Furber, A Aptecar, E Lafont, A Morelle, JF Slama, M Cazaux, P Boschat, J Rande, JLD TI Fractional flow reserve to guide coronary stent placement in routine: no efficacy of very high in-stent inflation pressure SO EUROPEAN HEART JOURNAL C1 Hop Henri Mondor, Unit Cardiol Intervent, Creteil, France. Hop De La Cavale Blanche, Dept Cardiol, Brest, France. Ctr Hosp Univ, Serv Radiol, Angers, France. Clin Fontaines, Pole Cardiovasc Intervent, Melun, France. Hop Georges Pompidou, Serv Cardiol, Paris, France. CHP St Martin, Unit Cardiol Intervent, Caen, France. Hop Antoine Beclere, Serv Cardiol, Clamart, France. Ctr Hosp Bretagne Sud, Serv Cardiol, Lorient, France. TC 0 PD SEP PY 2001 VL 22 SU Suppl. S BP 286 EP 286 UT ISI:000170988301090 ER PT J AU Prunier, F Furber, A Delepine, S Geslin, P TI Does intra-aortic balloon counterpulsation influence coronary blood flow velocity spectrum in patients with AMI without cardiogenic shock? SO EUROPEAN HEART JOURNAL C1 Univ Hosp Angers, Dept Cardiol, Angers, France. TC 0 PD SEP PY 2001 VL 22 SU Suppl. S BP 513 EP 513 UT ISI:000170988301967 ER PT J AU Beygui, F Furber, A Delepine, S Helft, G Da Costa, E Metzger, JP Geslin, P Le Jeune, JJ TI Evaluation of right ventricular volumes and function after primary angioplasty for acute myocardial infarction with fast cine magnetic resonance imaging SO EUROPEAN HEART JOURNAL C1 Hop Necker Enfants Malad, Paris, France. Angers Univ Hosp, Dept Cardiol, Angers, France. TC 0 PD SEP PY 2001 VL 22 SU Suppl. S BP 578 EP 578 UT ISI:000170988302215 ER PT J AU Levesque, A Bouteville, A TI Investigation of the tantalum chlorination with hydrogen chloride for LPCVD tantalum elaboration SO JOURNAL DE PHYSIQUE IV ID CHEMICAL-VAPOR-DEPOSITION; METALLIZATION; DIFFUSION; NITRIDE; COPPER; TA AB A chlorination process is presented in order to produce tantalum chloride. Solid tantalum is used as started material whereas the reducing agent is hydrogen chloride. Previous thermodynamic calculations allow to determine that the more convenient chlorination reaction temperature is 670 degreesC. Hydrogen chloride flow rate is varied from 10 to 40 sccm whereas the chlorination chamber is maintained at a total pressure ranging from 10 to 40 mbar. This process has been evaluated for depositing tantalum films by LPCVD through hydrogen reduction of the just formed tantalum chloride. Chlorination parameters such as HCl residence time, HCl partial pressure and chlorination chamber total pressure are investigated with respect to the tantalum weight deposited by LPCVD. By using the optimum set of parameters : HCl flow rate of 10 sccm, and total pressure of the chlorination chamber of 36 mbar, tantalum films are deposited at 800 degreesC and under a total pressure of 3.3 mbar at a deposition rate of 32.6 +/-2.9 mg/h that corresponds to 1.42 +/-0.09 mum/h. C1 LPMI, ENSAM, F-49035 Angers, France. RP Levesque, A, LPMI, ENSAM, 2 Blvd Ronceray, F-49035 Angers, France. TC 0 PD AUG PY 2001 VL 11 IS PR3 BP 907 EP 913 UT ISI:000171140300114 ER PT J AU Levesque, A Bouteville, A TI Evaluation of corrosion behaviour of tantalum coating obtained by low pressure chemical vapor deposition using electrochemical polarization SO JOURNAL DE PHYSIQUE IV AB Tantalum coatings are elaborated on titanium substrates through Low Pressure Chemical Vapor Deposition from tantalum pentachloride-hydrogen gaseous phase at a deposition temperature of 800 degreesC and a total pressure of 3.3 mbar. The aim of this paper is to evaluate the effectiveness of this tantalum coating in corrosive solution. Optical Microscopy and Scanning Electron Microscopy observations reveal that deposits are of 1.7 mum in thickness and conformal. The corrosion resistance of tantalum coated titanium substrates is quantified through standard potentiodynamic polarization method. Even for tantalum coatings exhibiting some defects as pores, the corrosion current density is as low as 0.25 mA/CM2. in very agressive solutions like kroll reagent (HNO3/HF). C1 LPMI, ENSAM, F-49035 Angers, France. RP Levesque, A, LPMI, ENSAM, 2 Blvd Ronceray, F-49035 Angers, France. TC 0 PD AUG PY 2001 VL 11 IS PR3 BP 915 EP 920 UT ISI:000171140300115 ER PT J AU Boisdron-Celle, N Lebouil, A Allain, P Gamelin, E TI Pharmacokinetic properties of platinium derivatives SO BULLETIN DU CANCER DE platinum derivatives; pharmacokinetics; oxaliplatin ID IMPAIRED RENAL-FUNCTION; CANCER-PATIENTS; ULTRAFILTERABLE PLATINUM; REPEATED COURSES; PHASE-I; CISPLATIN; OXALIPLATIN; CARBOPLATIN; DOCETAXEL; TOXICITY AB The three platinum derivatives currently available share many pharmacokinetic and pharmacodynamic (PK-PD) properties but present also some distinct characteristics, due to their structural differences, They result in different systemic PK-PD and metabolic behaviour and toxicity profile. Oxaliplatin is quickly, transformed into dach-platinum, the active metabolite, by loosing oxalate chain. Eighty to eighty-eight per cent of platinum are bound to proteins, as for cisplatin, whereas carboplatin is less reactive. Cisplatin and oxaliplatin active metabolites, i.e. monoaquo platin and dach-platin quickly react with small proteins with sulfhydryl groups, such as glutathione, cysteine and methionine, and then with high molecular weight proteins, such as albumin and gammaglobulins through covalent link. Thus, their terminal half lives are long, about ten days, but no platinum accumulation has been reported in plasma with oxaliplatin, whereas after cisplatin administration, both total and ultrafiltrable platinum progressively accumulate in plasma. This difference may play a role in the lack of oxaliplatin nephrotoxicity and its more delayed and reversible neurotoxicity. On the other hand, carboplatin is more stable, less bound to proteins and is largely excreted inchanged in urine. This can explain that it passes more easily through the blood brain barrier. Erythrocytes represent an important deep compartment, especially for oxaliplatin, a little bit less for cisplatin. Oxaliplatin is trapped in erythrocytes through a covalent binding to globin. There, its half life is identical to that of erythrocytes. According to certain authors, this trapping would be involved in the incidence of anemia. On the contrary, carboplatin is quickly extruded from erythrocytes. The three derivatives kinetics in plasma present a wide interindividual variability, resulting in differences in term of toxicity and efficacy. For the three of them, plasma clearance is correlated to creatinine clearance, but only carboplatin dosage can be individually adjusted, based on creatinine clearance measurement, thanks to its simple renal excretion, due to exclusive glomerular filtration, and after Calvert's, Egorin's and Chatelut's population kinetics studies. Cisplatin renal excretion is more complex, combining reabsorption and secretion processes. Therefore, individual dosage adjustment needs platinum concentration measurement in plasma, but there is no general agreement on the platinum species to measure, ultrafiltrable or bound. Oxaliplatin is too recent in clinical practice and still lacks of PK-PD data. These characteristics can help us for a better knowledge of the three platinum derivatives clinical properties, both in term of kinetics, behaviour and toxicity. C1 Ctr Paul Papin, Med Oncol Serv, F-49033 Angers 01, France. Ctr Paul Papin, Lab Oncopharmacol, F-49033 Angers, France. CHU Larrey, Pharmacol Lab, F-49033 Angers, France. RP Boisdron-Celle, N, Ctr Paul Papin, Med Oncol Serv, 2 Rue Moll, F-49033 Angers 01, France. TC 0 PD AUG PY 2001 VL 88 SI Sp. Iss. SI BP S14 EP S19 UT ISI:000171200800003 ER PT J AU Legrand, ER Chappard, D Basle, M Audran, M TI Trabecular bone microarchitecture is related to clinical risk factors in osteoporosis in men. SO JOURNAL OF BONE AND MINERAL RESEARCH C1 CHU Angers, Angers, France. TC 0 PD SEP PY 2001 VL 16 SU Suppl. 1 BP S217 EP S217 UT ISI:000170709000326 ER PT J AU Filmon, R Grizon, F Basle, MF Chappard, D TI Effect of charged groups (carboxymethyl) or alkaline phosphatase on the calcification of poly (2-hydroxyethyl) methacrylate. SO JOURNAL OF BONE AND MINERAL RESEARCH C1 LHEA, Fac Med, Angers, France. TC 0 PD SEP PY 2001 VL 16 SU Suppl. 1 BP S236 EP S236 UT ISI:000170709000408 ER PT J AU Libouban, H Moreau, M Basle, M Duquenne, M Bataille, R Chappard, D TI Increased bone remodeling due to ovariectomy dramatically increases tumoral growth in the 5T2 multiple myeloma mouse model. SO JOURNAL OF BONE AND MINERAL RESEARCH C1 INSERM, Nantes, France. LHEA, Fac Med, Angers, France. TC 0 PD SEP PY 2001 VL 16 SU Suppl. 1 BP S242 EP S242 UT ISI:000170709000431 ER PT J AU Lerouxel, E Libouban, H Moreau, MF Basle, MF Audran, M Chappard, D TI Preventive effects of a bisphosphonate (risedronate) on mandibular bone loss in an animal model of male osteoporosis (the orchidectomized rat). SO JOURNAL OF BONE AND MINERAL RESEARCH C1 Fac Med, LHEA, Angers, France. TC 0 PD SEP PY 2001 VL 16 SU Suppl. 1 BP S405 EP S405 UT ISI:000170709001121 ER PT J AU Jean, I Eyer, J Barthelaix-Pouplard, A Fressinaud, C TI Remyelination and axono-glial interactions in a demyelinating model using lysolecithin. SO JOURNAL OF NEUROCHEMISTRY C1 Univ Hosp, Cell Biol Lab, Angers, France. Univ Hosp, Dept Neurol, UPRES EA 3143, Angers, France. TC 0 PD SEP PY 2001 VL 78 SU Suppl. 1 BP 86 EP 86 UT ISI:000170789800304 ER PT J AU Walter, E Jaulin, L Kieffer, M TI Interval analysis for guaranteed and robust non-linear estimation in robotics SO NONLINEAR ANALYSIS-THEORY METHODS & APPLICATIONS DE interval analysis; outliers; robotics; robust estimation; state estimation; Stewart-Gough platform AB One of the challenges of interval analysis is to explore and bridge the gap between trivial illustrative examples for which it is not really needed and actual complicated applications for which it is still powerless. Two examples of applications pertaining to this gap are presented in this paper. The first one corresponds to the forward kinematic problem for a Stewart-Gough platform, a benchmark for numerical and symbolical computations. All real solutions are isolated in a guaranteed manner. The second example is relative to the localization and tracking of a vehicle in a partially known environment from distance measurements provided by sonars. The unavoidable presence of outliers is taken into account, which makes the method actually applicable. None of these problems can be solved satisfactorily by the usual local numerical methods based on iterative refinements, and the advantages provided by an approach based on interval analysis are evidenced. C1 UPS, SUPELEC, CNRS, Signaux & Syst Lab, F-91192 Gif Sur Yvette, France. Univ Angers, LISA, F-49045 Angers, France. RP Walter, E, UPS, SUPELEC, CNRS, Signaux & Syst Lab, F-91192 Gif Sur Yvette, France. TC 0 PD AUG PY 2001 VL 47 IS 1 PN Part 1 BP 191 EP 202 UT ISI:000170625400019 ER PT J AU Moal, F Vuillemin, E Wang, J Veal, N Oberti, E Gallois, Y Barriere, E Rousselot, D Cales, P TI Anti-fibrotic effects of a new liver specific NO donor in bile duct ligated rats SO JOURNAL OF HEPATOLOGY C1 Univ Angers, HIFIH Lab, Angers, France. TC 0 PD APR PY 2001 VL 34 SU Suppl. 1 BP 5 EP 6 UT ISI:000169243400016 ER PT J AU Croquet, V Oberti, F Moal, F Wang, J Roux, J Veal, N Cales, P TI Systemic and splanchnic effects of acute administration of losartan - A specific angiotensin II receptor antagonist - In normal and portal hypertensive rats SO JOURNAL OF HEPATOLOGY C1 Univ Angers, HIFIH Lab, Angers, France. TC 0 PD APR PY 2001 VL 34 SU Suppl. 1 BP 64 EP 64 UT ISI:000169243400218 ER PT J AU Veal, N Moal, F Oberti, F Vuillemin, E Cales, P TI Acute hemodynamic effects of vapreotide in cirrhotic rats SO JOURNAL OF HEPATOLOGY C1 Univ Angers, HIFIH Lab, Angers, France. TC 0 PD APR PY 2001 VL 34 SU Suppl. 1 BP 69 EP 69 UT ISI:000169243400238 ER PT J AU Aube, C Moal, F Croquet, V Thouveny, F Vuillemin, E Oberti, F Caron, C Cales, P TI Non invasive evaluation or liver fibrosis by in vivo MRI in rat SO JOURNAL OF HEPATOLOGY C1 Univ Angers, HIFIH Lab, Angers, France. TC 0 PD APR PY 2001 VL 34 SU Suppl. 1 BP 73 EP 73 UT ISI:000169243400253 ER PT J AU Croquet, V Moal, F Oberti, F Wang, J Roux, J Veal, N Vuillemin, E Gallois, Y Douay, O Cales, P TI Chronic hemodynamic and antifibrotic effects of a specific angiotensin II receptor antagonist (losartan) in bile duct ligated (BDL) rats and in portal vein ligated (PVL) rats SO JOURNAL OF HEPATOLOGY C1 Univ Angers, HIFIH Lab, Angers, France. TC 0 PD APR PY 2001 VL 34 SU Suppl. 1 BP 88 EP 88 UT ISI:000169243400312 ER PT J AU Wang, J Oberti, F Moal, F Croquet, V Vuillemin, E Roux, J Douay, O Gallois, Y Cales, P TI Amiloride prevents the development of splanchnic collateral circulation and liver fibrosis in rats with secondary biliary cirrhosis SO JOURNAL OF HEPATOLOGY C1 Univ Angers, HIFIH Lab, Angers, France. TC 0 PD APR PY 2001 VL 34 SU Suppl. 1 BP 104 EP 104 UT ISI:000169243400374 ER PT J AU Rodien, P TI Progressive disassembly of genes implicated in different forms of familial paraganglioma of the carotid glomus: is the mitochondria implicated in tumorigenesis? SO ANNALES D ENDOCRINOLOGIE ID MUTATIONS C1 CHU Angers, Serv Endocrinol, F-49033 Angers 01, France. RP Rodien, P, CHU Angers, Serv Endocrinol, 4 Rue Larrey, F-49033 Angers 01, France. TC 0 PD JUN PY 2001 VL 62 IS 4 BP 219 EP 219 UT ISI:000170212300001 ER PT J AU Le Gall, D Allain, P Aubin, G TI Associations and dissociations in frontal lobe syndrome: about arithmetical word problem solving and script arrangement deficits SO REVUE DE NEUROPSYCHOLOGIE DE frontal lobe syndrome; arithmetical word problem solving; script arrangement ID MENTAL REPRESENTATION; LESIONS; IMPAIRMENTS; DAMAGE AB In this work, we report three studies dealing with arithmetical word problem solving and script sorting and ordering in patients with frontal lobe damage. Following current theoretical models of frontal functioning, we hypothesized that these two types of tasks, which involved different cognitive processes, could reveal equivalent impairments of planning and selectivity. Dual-dissociations were observed in the two tasks. In the first experiment using arithmetical word problems, some patients had few planning disorders in problem solving but solved most of the unsolvable problems. We also identified patients which showed planning disorders but attacked the unsolvable problems. In the second experiment using script sorting and ordering, some patients performed correctly in sequencing the chronology of actions but failed to eliminate aberrant items. Other patients made errors in sequencing scripts but were able to inhibit aberrant actions. In the third study, we report the results of fifteen patients who participated to the first two studies. Data analyses revealed that their behavioral patterns of performances joined together, especially for selectivity impairment. Most of the patients who solved unsolvable problems also failed to eliminate aberrant items, and those who showed planning difficulties in problems also made errors in sequencing scripts. Some dissociations were also observed in classical executive tasks. While the dissociations validate a possible fractionation of some of the frontal lobe symptoms, the associations support the idea that tasks, which may tap distinct cognitive abilities, may involve comparable executive processes. C1 CHU Angers, Unite Neuropsychol, Dept Neurol, F-49033 Angers 01, France. Univ Angers, UFR Lettres Langues & Sci Humaines, Dept Psychol, Lab Psychol EA 2646, Angers, France. TC 0 PD JUN PY 2001 VL 11 IS 2 BP 299 EP 322 UT ISI:000170257000012 ER PT J AU Darniere, L TI Pseudo-algebraically closed rings SO MANUSCRIPTA MATHEMATICA ID FIELDS; INTEGERS AB We use a variant of the nonsingular Hasse Principle to define a class of rings analogeous to PAC fields, and prove that this class has nice model theoretical properties. Since there are a lot of examples of such rings which are of interest for arithmetical purpose we apply our study to those examples, obtaining several decidability results and one purely algebraic application. C1 Univ Angers, F-49045 Angers 01, France. RP Darniere, L, Univ Angers, 2 Bd Lavoisier, F-49045 Angers 01, France. TC 0 PD MAY PY 2001 VL 105 IS 1 BP 13 EP 46 UT ISI:000170339300002 ER PT J AU Cousseau, J Hudhomme, P Allard, E Gallego-Planas, N Cheng, FY TI New organo-C60 fullerene derivatives from dianion C60 2-. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY C1 Univ Angers, UMR CNRS 6501, IMMO Lab, F-49045 Angers, France. TC 0 PD APR 1 PY 2001 VL 221 PN Part 1 BP U589 EP U589 UT ISI:000168824703837 ER PT J AU Rocher, M TI Agrice and surfactants SO OCL-OLEAGINEUX CORPS GRAS LIPIDES DE non food; research and development; surfactant AB The scientific interest group AGRICE - Agriculture for Chemicals and Energy - focuses an new uses and enhanced value for agricultural products and byproducts as energy, chemical (surfactants) and materials feedstocks. AGRICE is committed to coordinating, funding, monitoring and evaluating research and development programmes that further these goals. Today surfactant's research-development programmes have been strengthened and fine-tuned, with a more direct focus on industry and markets. C1 Direct Agr & Bioenergies, F-49004 Angers 01, France. RP Rocher, M, Direct Agr & Bioenergies, 2 Sq Fayette,BP 406, F-49004 Angers 01, France. TC 0 PD MAR-APR PY 2001 VL 8 IS 2 BP 160 EP 160 UT ISI:000170174000012 ER PT J AU Genevieve, F Delisle, V Gardembas, M Foussard, C Gardais, J Zandecki, M TI Chronic (mature) lymphoid disorders in adult patients: chronic lymphocytic leukaemia and leukaemic phase of non-Hodgkin's lymphomas SO ANNALES DE BIOLOGIE CLINIQUE DE chronic lymphocytic leukaemia; non-Hodgkin's lymphoma; lymphocyte; morphology; diagnosis ID MANTLE-CELL LYMPHOMA; PROGNOSTIC FACTORS; PROLYMPHOCYTIC LEUKEMIA; VILLOUS LYMPHOCYTES; SPLENIC LYMPHOMA; INDOLENT COURSE; SCORING SYSTEM; CLASSIFICATION; MACROGLOBULINEMIA; DISEASES AB Chronic lymphocytic leukaemia is the most frequent haematological cancer in adult patients, and its incidence raises with aging. Diagnosis needs several clinical and biological data, but hemogram together with the morphological and immunophenotypic analysis of the lymphoid cells take the major place. If the diagnosis is performed easily in about 65% of the patients, various clinicobiological entities were reported in the past few years that must be identified, at least because some are of adverse prognosis. Moreover, the other chronic lymphoid neoplasms, corresponding to the various low and intermediate grade non-Hodgkin's lymphomas (mainly of follicular type, marginal zone, mantle cell zone), may disseminate within the blood and the bone marrow. Those circulating lymphoma cells must be identified at diagnosis in order to perforin the accurate diagnosis and to avoid an erroneous diagnosis of atypical chronic lymphocytic leukaemia. Up to 90% of lymphoid malignancies are B cell disorders, contrasting with only a few cases of T cell origin: some of those latter cases cannot be neglected however, as they may be observed in Western countries. Most recent classifications (REAL and WHO) defined all hematological malignancies: each entity referred to clinical, morphological, immunological, cytogenetic, and molecular findings. The basis of these classifications is pathophysiological, trying in each disorder to define a normal counterpart to, the pathological clone. Reviewing main steps of the immune response in the normal patient, corresponding to those involving B cells. it is possible indeed to localize and demonstrate a function for many of the cells that expand in lymphoid malignancies. C1 CHU Angers, Hematol Lab, F-49033 Angers 01, France. CHU Angers, Serv Malad Sang, F-49033 Angers, France. RP Genevieve, F, CHU Angers, Hematol Lab, 4 Rue Larrey, F-49033 Angers 01, France. TC 0 PD JUL-AUG PY 2001 VL 59 IS 4 BP 403 EP 415 UT ISI:000170087900004 ER PT J AU Helesbeux, JJ Seraphin, D Duval, O Richomme, P Bruneton, J TI Studies on the reactivity of a tertiary allylic alcohol in an acetophenonic series, a model for natural products synthesis SO JOURNAL OF PHARMACY AND PHARMACOLOGY ID COUMARINS; PLANTS AB The synthesis of benzopyranic simplified analogues of dibenzopyranic natural compounds is described, together with the access to a precursor of a new furobenzopyranic natural product. These natural products have anti-cancer activity. The 1,3-diacetoxy-2-acetyl-4-(3-hydroxy-3-methylbut-1-enyl)benzene synthone is used as a common precursor to these structures. C1 UFR Sci Pharmaceut & Ingn Sante, SONAS, F-49100 Angers, France. RP Duval, O, UFR Sci Pharmaceut & Ingn Sante, SONAS, 16 Blvd Daviers, F-49100 Angers, France. TC 0 PD JUL PY 2001 VL 53 IS 7 BP 955 EP 958 UT ISI:000170018700008 ER PT J AU Coutant, R Limal, M TI Complications of insulin-dependent diabetes in children - State of the art SO ARCHIVES DE PEDIATRIE ID GLYCOSYLATED HEMOGLOBIN; MICROALBUMINURIA; RETINOPATHY; MELLITUS; RISK C1 CHU Angers, Serv Pediat, F-49033 Angers 01, France. RP Coutant, R, CHU Angers, Serv Pediat, 4 Rue Larrey, F-49033 Angers 01, France. TC 0 PD MAY PY 2001 VL 8 SU Suppl. 2 BP 337S EP 339S UT ISI:000168997900043 ER PT J AU Regenet, N Pessaux, P Tuech, JJ Burtin, P du Plessis, R Arnaud, JP TI Abdominoperineal resection for locally recurrent rectal adenocarcinoma following low anterior resection. SO ANNALES DE CHIRURGIE DE abdominoperineal resection; local recurrence of rectal cancer; prognosis factors after local recurrence of rectal cancer; rectum ID INTRAOPERATIVE RADIATION-THERAPY; COLORECTAL-CANCER; PREOPERATIVE RADIOTHERAPY; REGIONAL RECURRENCES; SURGICAL-MANAGEMENT; CURATIVE RESECTION; ADJUVANT THERAPY; CARCINOMA; SURGERY; EXPERIENCE AB Study aim: The aim of this retrospective study was to evaluate the short and long term results of abdominoperineal resection for local recurrence following low anterior resection of a rectal adenocarcinoma and to determine the prognostic factors. Patients and methods: From January 1978 to December 1996, 35 patients (17 women, 18 men) with a mean age of 59.4 years, underwent an abdominoperineal resection for local recurrence after low anterior resection of a rectal adenocarcinoma. The primary tumor was below the peritoneum in 29 cases, and the mean security marge was 3 cm under the tumor. Tumor staging at the time of primary surgery included 23 Dukes B, 11 Dukes C, and 1 Dukes D. The mean time elapsed between low anterior resection and local recurrence was 16.4 months. The histological diagnosis of recurrence was obtained preoperatively in 29 cases (82.8%). Results: Resection was curative in 12 patients and palliative only in 23 patients. The recurrence was intramural in 3 cases, extramural in 10 cases, and mixed in 22 cases. Ten patients had an extended "en bloc" resection including one or several adjacent organs, and a synchronous metastasis was resected in 2 cases. The mortality rate was 2.8% (n=1) and the morbidity rate was 23% (n=8). The 1-year and 5-year survival rates were respectively 77 and 30.2% with the univariate analysis of prognosis factors of survival, there were four pretherapeutic factors (age, staging of the primary tumor, delay of the recurrence, CEA rate) and four therapeutic factors (curative resection, extramural recurrence, staging of the recurrence, postoperative radiotherapy). The curative or not curative type of resection was the only independent predictor of survival with multivariate analysis. Conclusion: The results of this study seem to justify an abdominoperinal resection for local recurrence after low anterior resection whenever possible. Long-term results may possibly be improved by using adjuvant treatment. (C) 2001 Editions scientifiques et medicales Elsevier SAS. C1 CHU Angers, Dept Chirurg Viscerale, F-49033 Angers 01, France. CHU Angers, Dept Gastroenterol, F-49033 Angers, France. RP Arnaud, JP, CHU Angers, Dept Chirurg Viscerale, 4 Rue Larrey, F-49033 Angers 01, France. TC 0 PD JUL PY 2001 VL 126 IS 6 BP 541 EP 548 UT ISI:000169750200006 ER PT J AU Eyer, J TI Investigating the role of neurofilaments in neurodegenerative processes using transgenic strategies SO JOURNAL OF NEUROCHEMISTRY C1 CHU Angers, UPRES EA3143, F-49033 Angers, France. TC 0 PD JUN PY 2001 VL 77 SU Suppl. 1 BP 13 EP 13 UT ISI:000169243300046 ER PT J AU Bouhanick, B Plun-Favreau, J Hadjadj, S Laboureau, S Laine-Cessac, P TI Inquiry into the distribution of drugs in a diabetic clinic SO THERAPIE DE diabetes; pharmacovigilance; drug prescription; drug delivery; medication error ID HOSPITALIZED-PATIENTS; EVENTS; ERRORS AB The aim of this study was to investigate possible discrepancies between the drug prescribed and that recorded in the patient's file. A prospective open blind study was conducted with 178 patients included consecutively. We analysed 1011 prescriptions (the median (range) number of drugs per patient was 5 (1-37)) and identified 49 discrepancies (5 per cent of cases). In 18 cases, the drug given to the patient by a nurse was not the drug initially prescribed but the drug recorded in the patient's file was the drug actually given to the patient. In another 31 cases, the drug given to the patient was not the drug initially prescribed, but the drug recorded was that prescribed. This inquiry shows that there may be a discrepancy between the drug initially prescribed and that actually administrated and suggests that poor traceability may affect pharmacological surveillance surveys. C1 CHU Angers, Serv Med B, F-49033 Angers 01, France. CHU Angers, Ctr Reg Pharmacovigilance, Angers, France. RP Bouhanick, B, CHU Angers, Serv Med B, F-49033 Angers 01, France. TC 0 PD MAY-JUN PY 2001 VL 56 IS 3 BP 315 EP 319 UT ISI:000169504300020 ER PT J AU Legrand, E Audran, M TI Bone microarchitecture in osteoporotic men: Information incomplete - Reply SO JOURNAL OF BONE AND MINERAL RESEARCH ID VERTEBRAL FRACTURES; MINERAL DENSITY C1 CHU Angers, Serv Rhumatol, F-49033 Angers, France. RP Legrand, E, CHU Angers, Serv Rhumatol, F-49033 Angers, France. TC 0 PD JUL PY 2001 VL 16 IS 7 BP 1375 EP 1375 UT ISI:000169491100024 ER PT J AU Regenet, N Kahn, X Leborgne, J Lehur, PA TI Colonic metastasis from renal cell carcinoma SO ANNALES DE CHIRURGIE DE colonic metastasis; renal cell carcinoma C1 CHU Nantes, Hotel Dieu, Clin Chirurg 2, F-44093 Nantes, France. RP Regenet, N, Inter CHU, Serv Chirurg Viscerale, 4 Rue Larrey, F-49033 Angers, France. TC 0 PD JUN PY 2001 VL 126 IS 5 BP 482 EP 483 UT ISI:000169312900016 ER PT J AU Derail, C Reveillere, M TI Correlation between sensorial analysis and color measurement. SO SCIENCES DES ALIMENTS DE colour; bread; flour; sensory analysis; colorimeter ID DELICIOUS APPLE STRAINS; CHROMATICITY AB Colour measurement is becoming increasingly important in quality control in the food industry and the technology is making it simpler to perform. Commercialy, it is very important to measure this parameter. But today, the colour is appreciated by sensorial analysis by the bakers without a proper background and without reference to the parameters of the colours. We propose a method to measure the colour and to compare its parameters to the results of sensory analysis by the bakers. The results obtained allow us to propose a reliable experimental method of the measure of the colour. A good relation between the sensory analysis of the bakers and the results of the colour measurement has been found. One of the principal aim of this work is to control better the measure of the colour in order to use this parameter in the choice of the flour for a given application. C1 Ecole Super Agr Angers, Grp Rech Agroind Prod Proc & Environm, F-49007 Angers, France. RP Derail, C, Univ Pau & Pays Adour, Lab Phys Mat Ind, F-64013 Pau, France. TC 0 PY 2001 VL 21 IS 1 BP 49 EP 64 UT ISI:000169225400004 ER PT J AU Felix, Y Halperin, S Thomas, JC TI The Serre spectral sequence of a multiplicative fibration SO TRANSACTIONS OF THE AMERICAN MATHEMATICAL SOCIETY DE multiplicative fibration; loop space; Hopf algebra; Serre spectral sequences; elliptic space ID LOOP SPACE HOMOLOGY; HOPF-ALGEBRAS; CATEGORY; TORSION; MODULE AB In a fibration OmegaF (Omegaj)under right arrow OmegaX (Omega pi )under right arrow OmegaB we show that finiteness conditions on F force the homology Serre spectral sequence with F-p-coefficients to collapse at some finite term. This in particular implies that as graded vector spaces, H-*(OmegaX) is "almost" isomorphic to H-*(OmegaB) circle times H-*(OmegaF). One consequence is the conclusion that X is elliptic if and only if B and F are. C1 Univ Louvain, Inst Math, B-1348 Louvain, Belgium. Univ Maryland, Coll Comp Math & Phys Sci, College Pk, MD 20742 USA. Univ Angers, Fac Sci, F-49045 Angers, France. RP Felix, Y, Univ Louvain, Inst Math, B-1348 Louvain, Belgium. TC 0 PY 2001 VL 353 IS 9 BP 3803 EP 3831 UT ISI:000168998400018 ER PT J AU Rouge-Maillart, C Boasson, M Riche, P Penneau, M TI Organ donation between family members: an ethical dilemma imposed by conflicting regulations SO PRESSE MEDICALE AB Under specific conditions, French law authorizes organ donation despite the donor's seropositivity for certain infectious conditions. The recipient must however be informed of the potential risk of graft-related infection. Anonymous donation being the rule, the rights of the donor are respected since his/her serological status remains a medical secret. However, an exception to the rule of anonymous donation is allowed for organ donation between family members. In such a situation-often justified by the urgent nature of the transplantation-the donor's serological status would have to be revealed to the recipient, breaking the rule of medical secrecy. The physician who breaks the rule is simply implementing legal regulations (with the subsequent protection against any penal or disciplinary measures) but nevertheless performs an ethically questionable act. The recompense for donation would be an incongruent violation of personal rights. At the present rime, there does not appear to be a satisfactory solution to this dilemma. The only solution that could be put forward would be to ask the donor to voluntarily inform the recipient of his/her seropositivity. C1 CHU Angers, Serv Urgences, F-49033 Angers 01, France. CHU Angers, Serv Greffes Moelle & Malad Sang, F-49033 Angers, France. RP Rouge-Maillart, C, CHU Angers, Serv Urgences, 4 Rue Larrey, F-49033 Angers 01, France. TC 0 PD MAY 19 PY 2001 VL 30 IS 18 BP 909 EP 910 UT ISI:000168920700011 ER PT J AU Menichi, L TI p-th powers in mod p cohomology of fibers SO COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE I-MATHEMATIQUE AB Let k be a non-negative integer. Let F hooked right arrow E --> B be a fibration whose base space B is a finite simply-connected CW-complex of dimension less than or equal to p(k) and whose total space E is a path-connected CW-complex of dimension less than or equal to p(k) - 1. If alpha is an element of H+ (F; F-P,) then alpha (p k) = 0. (C) 2001 Academie des sciences/Editions scientifiques et medicales Elsevier SAS. C1 Univ Angers, Fac Sci, F-49045 Angers, France. RP Menichi, L, Univ Angers, Fac Sci, 2 Blvd Lavoisier, F-49045 Angers, France. TC 0 PD MAR 15 PY 2001 VL 332 IS 6 BP 537 EP 540 UT ISI:000168953300010 ER PT J AU Lerouxel, E Libouban, H Moreau, MF Legrand, E Basle, MF Audran, M Chappard, D TI Mandibular bone loss in an animal model of male osteoporosis (the orchidectomized rat): A radiographic and densiometric study SO BONE C1 CHU Angers, Angers, France. Univ Angers, Angers, France. TC 0 PD MAY PY 2001 VL 28 IS 5 SU Suppl. S BP S248 EP S248 UT ISI:000168825000797 ER PT J AU Guy, I Charles, B Guinaudeau, H Fournet, A Ferreira, ME de Arias, AR TI Essential oils from leaves of two Paraguayan Rutaceae: Zanthoxylum hyemale A. St. Hil. and Z-naranjillo Griseb. SO JOURNAL OF ESSENTIAL OIL RESEARCH DE Zanthoxylum hyemale; Zanthoxylum naranjillo; Rutaceae; essential oil composition; linalool; beta-elemene; bicyclogermacrene; spathulenol; beta-caryophyllene; germacrene D; caryophyllene oxide AB Aerial parts of the species Zanthoxylum hyemale and Zanthoxylum naranjillo which present the same botanical apparence were subjected to hydrodistillation. Twenty nine compounds have been identified by GC/MS analysis of the essential oils. Sesquiterpenes such as spathulenol and bicyclogermacrene are the main components. C1 Dept Soc & Sante, Inst Rech Dev, F-75480 Paris 10, France. Univ Angers, Fac Pharm, F-49045 Angers, France. Inst Invest Ciencias Salud, Dept Trop Med, Asuncion, Paraguay. RP Fournet, A, Dept Soc & Sante, Inst Rech Dev, 213 Rue La Fayette, F-75480 Paris 10, France. TC 0 PD MAY-JUN PY 2001 VL 13 IS 3 BP 200 EP 201 UT ISI:000168774600019 ER PT J AU Chapeau-Blondeau, F Monir, A TI Generation of signals with long-range correlations SO ELECTRONICS LETTERS AB The authors propose a model in the Term of a first-order recurrence which is capable of generating, over potentially unlimited ranges, long-range correlations of power-law decay with a controllable exponent. C1 Univ Angers, LISA, F-49000 Angers, France. RP Chapeau-Blondeau, F, Univ Angers, LISA, 62 Ave Notre Dame Lac, F-49000 Angers, France. TC 0 PD APR 26 PY 2001 VL 37 IS 9 BP 599 EP 600 UT ISI:000168646600037 ER PT J AU Gaci, M Morel, O Sultan, AM Giraud, P Muratet, JP Gamelin, E Jallet, P TI False-positive suspicion of a thyroid metastatic lesion by In-111 pentetreotide uptake in a toxic nodular goiter SO CLINICAL NUCLEAR MEDICINE DE carcinoid tumor; false-positive uptake; indium-111 pentetreotide; toxic nodular goiter ID SOMATOSTATIN-RECEPTOR SCINTIGRAPHY; TUMOR C1 Ctr Paul Papin, F-49033 Angers 01, France. RP Gaci, M, Ctr Paul Papin, 2 Rue Moll, F-49033 Angers 01, France. TC 0 PD JUN PY 2001 VL 26 IS 6 BP 579 EP 581 UT ISI:000168615100032 ER PT J AU Kuribayashi, K TI The S-1-equivariant cohomology of homogeneous spaces as deformations of algebras SO BULLETIN OF THE LONDON MATHEMATICAL SOCIETY AB Let G be a compact connected Lie group and K a maximal rank subgroup of G. The homogeneous space G/K has the S-1-action defined by left translations induced from a homomorphism from S-1 to G. In this paper, we study a problem on the realization of some deformation of the cohomology algebra H-*(G/K;F-p) by the S-1-equivariant cohomology of G/K. In consequence, for the case where G is a classical Lie group, it follows that there exists at most one essentially different homomorphism from S-1 to G which realizes a given deformation, and that the homomorphism is controlled by an appropriate equation in one indeterminate. C1 Okayama Univ Sci, Dept Math Appl, Okayama 7000005, Japan. RP Kuribayashi, K, Univ Angers, Unite Algebre & Geometrie, Blvd Lavoisier, F-49045 Angers, France. TC 0 PD JAN PY 2001 VL 33 PN Part 1 BP 89 EP 99 UT ISI:000168366900013 ER PT J AU Guy, I Charles, B Guinaudeau, H Ferreira, ME de Arias, AR Fournet, A TI Essential oils from Zanthoxylum chiloperone and Z-riedelianum growing in Paraguay SO PHARMACEUTICAL BIOLOGY DE Rutaceae; Zanthoxylum chiloperone var. angustifolium Engl.; Z. riedelianum Engl.; essential oil; GC/MS method; Paraguay AB Essential oils from aerial parts of Zanthoxylum chiloperone var. angustifolium Engl. and Z. riedelianum Engl. have been analyzed by GC/MS. Twenty-four compounds were identified, representing 80% of essential oils. The main components of these essential oils are, respectively, cis-nerolidol (71.0%) and spathulenol (65.2%). C1 Dept Soc & Sante, Inst Rech Dev, F-75480 Paris 10, France. Univ Angers, Fac Pharm, Angers, France. Inst Invest Ciencias Salud, Dept Trop Med, Asuncion, Paraguay. RP Fournet, A, Dept Soc & Sante, Inst Rech Dev, 213 Rue La Fayette, F-75480 Paris 10, France. TC 0 PY 2001 VL 39 IS 2 BP 152 EP 154 UT ISI:000168326000010 ER PT J AU Duveau, E Pavie, F Douay, O Ginies, JL TI Benign transient hyperphosphatasemia of early chilhood SO ARCHIVES DE PEDIATRIE DE alkaline phosphatase; child ID CHILDHOOD; INFANCY C1 CHU Angers, Dept Pediat, F-49033 Angers, France. CHU Angers, Biochim Lab, F-49033 Angers, France. RP Duveau, E, CHU Angers, Dept Pediat, F-49033 Angers, France. TC 0 PD APR PY 2001 VL 8 IS 4 BP 445 EP 445 UT ISI:000168255500016 ER PT J AU Picquet, J Jousset, Y Papon, X Brillu, C Villapadierna, F Enon, B TI Bypass to the descending artery of the knee for critical limb ischaemia SO EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY DE critical limb ischaemia; bypass; descending artery of the knee ID CRITICAL LEG ISCHEMIA C1 Angers Univ Hosp, Dept Cardiovasc & Thorac Surg, F-49033 Angers 01, France. RP Picquet, J, Angers Univ Hosp, Dept Cardiovasc & Thorac Surg, 4 Rue Larrey, F-49033 Angers 01, France. TC 0 PD MAR PY 2001 VL 21 IS 3 BP 276 EP 278 UT ISI:000168100200015 ER PT S AU Guerin, F Dumon, B Hambli, R Tebbi, O TI Accelerated testing based on a mechanical-damage model SO ANNUAL RELIABILITY AND MAINTAINABILITY SYMPOSIUM, 2001 PROCEEDINGS SE PROCEEDINGS : ANNUAL RELIABILITY AND MAINTAINABILITY SYMPOSIUM DE accelerated testing; crack propagation; mechanical damage; loading stress; acceleration law AB This article presents a method of accelerated testing for products under stress in order to estimate their level of reliability under normal conditions; To this end, we suggest an accelerated testing law testing based on a mechanical model of damage in order to be close to the failure mechanism. In particular, we propose a method based on a model of crack propagation under stress. C1 ISTIA, Qual & Reliabil Dept, F-49000 Angers, France. RP Guerin, F, ISTIA, Qual & Reliabil Dept, 62 Av Notre Dame du Lac, F-49000 Angers, France. TC 0 PY 2001 BP 372 EP 376 UT ISI:000167729400063 ER PT J AU Le Normand, L Glemain, P Cordonnier, JP Brunel, P Karam, G Boullanger, P Buzelin, JM TI Accuracy of pressure measurements obtained by a new rectal balloon catheter SO PROGRES EN UROLOGIE DE urodynamic; rectal catheter; abdominal pressure; urodynamic equipment ID INTRA-ABDOMINAL PRESSURE AB The accuracy of measurements performed by a balloon catheter used to record abdominal pressure during urodynamic investigations was verified on a test bench. Objective: To study the accuracy and precision of the pressure measurements obtained with a new rectal balloon catheter (ref. 95018 Laboratoires Vermed (R)), designed to record abdominal pressure during urodynamic assessments. The clinical value of this catheter, using air for pressure transmission, is its simplicity, as there is no contamination of the perfusion circuit or transducer, no purging of the circuit and artefacts related to movements of the tubing are eliminated Method:: The catheter was placed in a pressure chamber fitted with a precise, calibrated regulation system allowing programmed pressure variations from 10 to 150 cmH2O. Pressures recorded by the test catheter were compared to reference pressures applied to the chamber The frequency of acquisition of pressure measurements was 100 Hertz and the resolution was 10 Hertz. This model was used to study the accuracy of pressure measurements and the response times of the catheter. Measurements were performed with a volume of 2 ml of air introduced into the catheter (volume recommended by the manufacturer), and the optimal volume was investigated by inflating the catheter until the best result was obtained. The evaluation rr as based on calculation of the mean difference observed between the two measurements and the scatter of the differences observed. Results: When the catheter was filled with 2 ml of air, pressure measured by the catheter were overestimated an average of 1.1 cmH2O (standard deviation = 1), and 95% of the differences between the two measurements were within +/- 2.15 cmH2O. The optimal air volume was found to be 1.5 ml. With this air volume, no significant difference was observed between the two measurements. The mean observed difference was 0.2 cmH2O (SD = 1.2), which means that 95% of the differences were situated within the range of +/- 2.35 cmH2O. Conclusion: The pressure recording method with this new catheter is validated in terms of physical parameters. C1 CHU Nantes, Urol Clin, F-44093 Nantes 01, France. Ecole Cent, Lab Mecan Fluides, Nantes, France. CHU Angers, Lab Urodynam, Angers, France. RP Le Normand, L, CHU Nantes, Urol Clin, F-44093 Nantes 01, France. TC 0 PD FEB PY 2001 VL 11 IS 1 BP 127 EP 131 UT ISI:000167584800033 ER PT J AU Saumet, JL TI Involvement of CGRP but not tachykinins in local pressure-induced vasodilation SO FASEB JOURNAL C1 Univ Angers, Physiol Lab, F-49045 Angers, France. TC 0 PD MAR 7 PY 2001 VL 15 IS 4 PN Part 1 BP A475 EP A475 UT ISI:000167438102714 ER PT J AU Granry, JC Le Rolle, T TI Is autologous transfusion still a current topic? SO ANNALES FRANCAISES D ANESTHESIE ET DE REANIMATION C1 CHU Angers, Dept Anesthesie Reanimat, F-49033 Angers, France. RP Granry, JC, CHU Angers, Dept Anesthesie Reanimat, 4 Rue Larrey, F-49033 Angers, France. TC 0 PD JAN PY 2001 VL 20 IS 1 BP 7 EP 8 UT ISI:000167124800001 ER PT J AU Derkowska, B Sahraoui, B Phu, XN Andraud, C TI The third-order nonlinear optical susceptibility in octupolar molecule and its dipolar subunit SO ACTA PHYSICA POLONICA A ID TETRATHIAFULVALENE DERIVATIVES AB The third-order nonlinear optical susceptibility chi ((3)) of octupolar molecule with three double bonds was measured using degenerate four wave mixing technique at 532 nm in tetrahydrofuran solution. For comparison, we also measured its analogous dipolar subunit. We found that the chi ((3)) value for octupolar molecule is about twice larger than its dipolar subunit. C1 Univ Angers, Lab POMA, CNRS, EP 130, F-49045 Angers 01, France. Ecole Normale Super Lyon, CNRS, Stereochim & Interact Mol UMR, F-69364 Lyon, France. Nicholas Copernicus Univ, Inst Phys, PL-87100 Torun, Poland. RP Sahraoui, B, Univ Angers, Lab POMA, CNRS, EP 130, 2 Bd Lavoisier, F-49045 Angers 01, France. TC 0 PD JAN PY 2001 VL 99 IS 1 BP 155 EP 160 UT ISI:000166996800017 ER PT J AU Goumy, L TI Idiopathic juvenile polyarthritis SO PRESSE MEDICALE ID RHEUMATOID-ARTHRITIS; TRIAMCINOLONE HEXACETONIDE; CHILDREN; METHOTREXATE; MULTICENTER; ANTIBODIES; UVEITIS AB Definition: Idiopathic juvenile polyarthritis includes a group of inflammatory diseases that affect at least five joints, either from onset or within the first six months of the disease course in children under 16 years of age. Diagnosis is arrived at by elimination. Besides malignant disease, always to be considered as a differential diagnosis, idiopathic juvenile polyarthritis can be divided into rheumatoid factor (RF)-positive and RF-negative polyarthritis and extended forms of oligoarthritis. RF-negative polyarthritis: Antinuclear antibody (ANA)-positive polyarthritis must be distinguished from extended forms of oligoarthritis which are also ANA-positive. ANA-positive polyarthritis generally begins early, at the age of 2 or 3 years, predominantly in girls. Characteristic torpid uveitis is frequent, requiring regular systematic screening. Joints are minimally painful with symmetrical involvement usually of the knees and wrists. Progressive joint destruction and/or growth disorders are common. The disease progresses by acute episodes. Nonsteroidal antiinflammatory drugs are used, associated with a specific treatment and local care as needed. General corticosteroids may be required in certain cases but should be avoided if possible. Seronegative forms: Certain patients have no detectable antibodies. These patients generally have fewer ocular problems and less severe joint disease. The treatment is the same as in ANA-positive forms. RF-positive polyarthritis: RF-positive polyarthritis is exceptional and occurs early in young girls. Specialized care: Irrespective of the type of disease, all children with idiopathic juvenile polyarthritis require multidisciplinary specialized care for their chronic and severe, potentially invalidating disease. C1 CHU Angers, Serv Rhumatol, F-49033 Angers 01, France. CHU Angers, Serv Pediat, F-49033 Angers, France. RP Goumy, L, CHU Angers, Serv Rhumatol, F-49033 Angers 01, France. TC 0 PD JAN 27 PY 2001 VL 30 IS 3 BP 137 EP 142 UT ISI:000166781200012 ER PT J AU Duly-Bouhanick, B Menard, S Hadjadj, S Soares-Barbosa, S Plun-Favreau, J Guilloteau, G TI Prevention of cardiovascular risk in patients with type 2 diabetes mellitus with aspirin SO PRESSE MEDICALE ID DISEASE AB Theory and reality: Diabetes mellitus is known to be associated with excess cardiovascular risk. Prescription of antiplatelet agents such as acetylsalicylic acid would thus appear to be warranted. That is the theory, but the reality is much different. A review of the literature provides evidence on the use of acetylsalicylic acid for primary and secondary preventive care, but conclusions are often extrapolated from studies conducted in the general population. Evidence of a beneficial effect in diabetics: The HOT study, conducted in hypertensive patients) demonstrated that acetylsalicylic acid at the dose of 75 mg a day, reduced the rate of major cardiovascular events by 15% (p = 0.03) and of myocardial infarction by 36% (p = 0.02) with no effect on stroke. In diabetic patients (n = 1500), the benefit was even more pronounced. Risks: The risk of bleeding must be balanced against the beneficial cardiovascular effect. Diabetic retinopathy is not aggravated by aspirin. The data reported in the literature do not however enable any evidenced-based decision on dosing for the diabetic population with numerous cardiovascular risks. C1 CHU Angers, Serv Med B, F-49033 Angers 01, France. RP Duly-Bouhanick, B, CHU Angers, Serv Med B, F-49033 Angers 01, France. TC 0 PD JAN 20 PY 2001 VL 30 IS 2 BP 87 EP 91 UT ISI:000166685900013 ER EF